阿司匹林诱导大鼠肠道损伤模型的构建

陈勇江; 王金荣; 苏兰利; 高温婷; 赵银丽; 段二珍; 王晋晋; 娄鹏

doi:10.3969/j.issn.1006-267x.2020.02.045

动物营养学报 >

2020 , Vol. 32 >Issue 2: 898 - 904

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.045

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

阿司匹林诱导大鼠肠道损伤模型的构建

陈勇江 ,
王金荣 ,
苏兰利 ,
高温婷 ,
赵银丽 ,
段二珍 ,
王晋晋 ,
娄鹏

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1. 河南工业大学生物工程学院, 郑州 450001;
2. 周口市动物卫生监督所, 周口 466000

陈勇江(1995-),男,河南新乡人,硕士研究生,研究方向为动物营养和饲料科学。E-mail:1559899363@qq.com

收稿日期: 2019-07-29

网络出版日期: 2020-02-21

基金资助

国家自然科学基金项目（U1604106，31702235）

收起

Establishment of Intestinal Injury Model Induced by Aspirin in Rats

CHEN Yongjiang ,
WANG Jinrong ,
SU Lanli ,
GAO Wenting ,
ZHAO Yinli ,
DUAN Erzhen ,
WANG Jinjin ,
LOU Peng

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1. College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China;
2. Zhoukou Animal Health Supervision, Zhoukou 466000, China

Received date: 2019-07-29

Online published: 2020-02-21

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摘要

本试验旨在建立阿司匹林诱导的大鼠肠道损伤模型。试验采用单因素试验设计，选用6周龄SD大鼠18只，经过7 d的适应性饲养后随机分为3个组，每组6只，单笼饲喂。模型1组和模型2组阿司匹林的灌胃剂量分别为50和200 mg/kg，空白对照组灌胃等量生理盐水，持续灌胃14 d。结果表明：与空白对照组相比，灌服50 mg/kg阿司匹林显著降低了大鼠的体增重、血清白细胞介素-2（IL-2）含量、肠道黏膜分泌性免疫球蛋白A（sIgA）含量、肠道绒毛高度和绒毛高度与隐窝深度的比值（P<0.05），但对肝脏指数、脾脏指数和血清溶菌酶（LZM）活性没有显著影响（P>0.05）；灌服200 mg/kg阿司匹林显著降低了大鼠的体增重、肝脏指数、血清IL-2含量与LZM活性、肠道黏膜sIgA含量、肠道绒毛高度和隐窝深度及二者的比值（P<0.05）。在本试验条件下，采用200 mg/kg剂量的阿司匹林连续灌胃14 d可以成功建立大鼠的肠道损伤模型。

关键词： 阿司匹林; 大鼠; 肠道损伤; 模型建立

本文引用格式

陈勇江 , 王金荣 , 苏兰利 , 高温婷 , 赵银丽 , 段二珍 , 王晋晋 , 娄鹏 . 阿司匹林诱导大鼠肠道损伤模型的构建[J]. 动物营养学报, 2020 , 32(2) : 898 -904 . DOI: 10.3969/j.issn.1006-267x.2020.02.045

Abstract

The purpose of this study was to establish an intestinal injury model of rats which induced by aspirin. Single factor test was designed in the experiment, eighteen 6-week-old SD rats were randomly divided into 3 groups with 6 rats in each group, and the rats were feed separately. The dosage of aspirin was 50 and 200 mg/kg by intragastric injection administration in model 1 group and model 2 group, respectively, and the control group was given normal saline of the same quantity. The adjusted period was 7 days, and the formal period was 14 days. The results showed as follows:compared with the blank control group, orally administered 50 mg/kg aspirin significantly decreased body weight gain (BWG), serum interleukin-2 (IL-2) content, intestinal mucosa secretory immunoglobulin A (sIgA) content, intestinal villi height (VH), VH to crypt depth (CD) ratio (VH/CD) (P<0.05), but had no significant effects on liver index, spleen index and serum lysozyme (LZM) activity (P>0.05); orally administered 200 mg/kg aspirin significantly decreased significantly decreased BWG, serum IL-2 content and LZM activity, intestinal mucosa sIgA content, intestinal VH, CD and VH/CD (P<0.05). In conclusion, the intestinal injury model of rats is successfully established by 200 mg/kg aspirin continued gavage for 14 d under this experimental condition.

Key words： aspirin; rats; intestinal injury; model establishment

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