本试验旨在研究发酵银杏叶对斜带石斑鱼生长性能、血浆生化指标与肝脏抗氧化指标的影响。配制添加不同水平[0（对照）、0.25%、0.50%]发酵银杏叶的饲料，投喂初始体重为12.6 g的斜带石斑鱼8周，每种饲料投喂3个网箱（重复），每个网箱投放30尾鱼。结果显示：1）生长性能指标各组间均没有显著差异（P>0.05）。2）与对照组相比，饲料中添加0.25%或0.50%发酵银杏叶均可显著降低血浆谷丙转氨酶（ALT）活性（P<0.05）；饲料中添加0.50%发酵银杏叶还可显著降低血浆谷草转氨酶（AST）活性（P<0.05）。3）与对照组相比，饲料中添加0.25%或0.50%发酵银杏叶均可显著降低肝脏丙二醛（MDA）含量（P<0.05），显著提高肝脏超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性（P<0.05）；饲料中添加0.50%发酵银杏叶还可显著提高肝脏过氧化氢酶（CAT）活性（P<0.05）。由此可见，饲料中添加0.25%或0.50%发酵银杏叶对斜带石斑鱼的生长性能无负面影响，还可提高斜带石斑鱼的肝脏抗氧化能力，且添加水平为0.50%时效果优于添加水平为0.25%时。
This experiment was conducted to study the effects of fermented Ginkgo biloba leaves on growth performance, plasma biochemical parameters and liver antioxidant parameters of orange-spotted grouper (Epinephelus coioides). Orange-spotted grouper with the initial body weight of 12.6 g were cultured for 8 weeks by adding different levels[0 (control), 0.25% and 0.50%] of fermented Ginkgo biloba leaves to the diet. Each diet designed 3 net cages (replicates) and each net cage cultured 30 fish. The results showed as follows:1) there were no differences in the growth indexes such as weight gain rate, specific growth rate and hepatosomatic index among groups (P>0.05). 2) Compared with the control group, adding 0.25% or 0.50% fermented Ginkgo biloba leaves in diets could significantly reduce the activity of alanine aminotransferase (ALT) in serum (P<0.05), and adding 0.50% fermented Ginkgo biloba leaves in diets also could significantly reduce the activity of aspartate aminotransferase (AST) in serum (P<0.05). 3) Compared with the control group, adding 0.25% or 0.50% fermented Ginkgo biloba leaves in diets could significantly reduce the liver malondialdehyde (MDA) content and significantly increase the activities of liver superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.05), and adding 0.50% fermented Ginkgo biloba leaves in diets also could significantly increase the activity of liver catalase (CAT) (P<0.05). Therefore, adding 0.25% or 0.50% fermented Ginkgo biloba leaves in diet has no negative effect on the growth performance of orange-spotted grouper, but it can improve the liver antioxidant capacity of orange-spotted grouper, and the effect is better than 0.25% when the supplemental level is 0.50%.
[1] 林超辉.斜带石斑鱼的生物学特性及养殖技术[J].齐鲁渔业,2008(2):22-23.
[2] 罗鸣,陈傅晓,刘龙龙,等.我国石斑鱼养殖疾病的研究进展[J].水产科学,2013,32(9):549-554.
[3] 柳闽生,陈晔,徐常龙.银杏叶有效成分的研究与资源的开发利用[J].江西林业科技,2006(2):28-31.
[4] 王雁,杨义芳.银杏叶的药理作用及其机制的研究进展[J].中国现代应用药学,2001,18(1):1-4.
[5] 梁灵君,王洪新,孙雪芳,等.Toll样受体4/NF-κB信号通路参与黄芪多糖对肿瘤坏死因子α诱导的乳大鼠心肌细胞肥大的抑制作用[J].中国药理学与毒理学杂志,2013,27(2):168-173.
[6] 周怡,耿越.多糖与细胞凋亡关系探讨[J].中草药,2007,38(4):623-626.
[7] 成喜雨,崔馨,刘春朝,等.中草药抗氧化活性研究进展[J].天然产物研究与开发,2006,18(3):514-518.
[8] 李羿,刘忠荣,吴洽庆,等.发酵中药——拓展中药新药研究开发的新空间[J].天然产物研究与开发,2004,16(2):179-181,184.
[9] 杨芳,赵崇妍,屈青松,等.银杏叶发酵研究进展[J].中国现代中药,2018,20(8):1034-1038.
[10] 逯岩.发酵银杏叶在团头鲂幼鱼饲料中的应用研究[D].硕士学位论文.南京:南京林业大学,2015.
[11] 廖石勇,邹坪珈,李琦,等.银杏叶-辣木叶发酵物对异育银鲫生长发育、消化酶活性以及抗氧化能力的影响[J].黑龙江畜牧兽医,2018(14):176-179.
[12] 李泽鑫,宋凯,鲁康乐,等.发酵中药制剂对石斑鱼肝细胞氧化损伤的保护作用[J].黑龙江畜牧兽医,2018(14):171-175.
[13] TAN X H,SUN Z Z,LIU Q Y,et al.Effects of dietary ginkgo biloba leaf extract on growth performance,plasma biochemical parameters,fish composition,immune responses,liver histology,and immune and apoptosis-related genes expression of hybrid grouper (Epinephelus lanceolatus×Epinephelus fuscoguttatus ♀) fed high lipid diets[J].Fish & Shellfish Immunology,2018,72:399-409.
[14] YAN Z,FAN R,YIN S,et al.Protective effects of Ginkgo biloba leaf polysaccharide on nonalcoholic fatty liver disease and its mechanisms[J].International Journal of Biological Macromolecules,2015,80:573-580.
[15] JEONG H S,KIM K H,LEE I S,et al.Ginkgolide A ameliorates non-alcoholic fatty liver diseases on high fat diet mice[J].Biomedicine & Pharmacotherapy,2017,88:625-634.
[16] 杜磊,王昌源,张立华,等.肝功指标判断汉坦病毒所致肝损害的临床价值[J].中国病原生物学杂志,2008,3(10):740-742.
[17] 张硕,洪波,王庆林,等.4种黄酮类化合物对CCl4致小鼠肝纤维化的防治作用[J].华西药学杂志,2012,27(2):141-143.
[18] 刘存歧,王伟伟,张亚娟.水生生物超氧化物歧化酶的酶学研究进展[J].水产科学,2005,24(11):49-52.
[19] 吴迪,刘平平,李萌,等.葛根水提液及葛根发酵液的体外抗氧化及抗衰老功效评价[J].食品工业科技,2019,40(12):285-290,294.
[20] 瞿春莹,李定国,汪余勤,等.壳聚糖对胃溃疡大鼠血清中MDA、SOD及GSH-PX的影响[J].上海医药,2008,29(5):219-222.
[21] 曹建,邓美玲,梁应平,等.银杏叶提取物经门静脉预处理对肝脏手术中氧化应激的影响[J].临床麻醉学杂志,2017,33(7):652-655.
[22] 太海燕,张善玉,朴莲荀.银杏叶提取物对小鼠急性酒精性肝损伤的保护作用[J].延边大学医学学报,2013,36(4):268-270.
[23] NIU Y,WAN X Y,ZHANG L L,et al.Effect of different doses of fermented Ginkgo biloba leaves on serum biochemistry,antioxidant capacity hepatic gene expression in broilers[J].Animal Feed Science and Technology,2019,248:132-140.
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