动物营养学报 >

2020 , Vol. 32 >Issue 3: 1109 - 1117

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.017

猪营养与饲料 SWINE NUTRITION AND FEED

饲喂人工乳对14日龄断奶仔猪生长性能、肠道抗氧化能力及屏障功能的影响

  • 辛怀璐 ,
  • 蒋俊劼 ,
  • 陈代文 ,
  • 何健 ,
  • 李元凤 ,
  • 余冰 ,
  • 何军 ,
  • 黄志清 ,
  • 毛湘冰 ,
  • 虞洁 ,
  • 罗玉衡 ,
  • 罗钧秋 ,
  • 郑萍
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  • 1. 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 雅安 625014;
    2. 西南科技大学生命科学与工程学院, 绵阳 621010;
    3. 四川铁骑力士集团冯光德实验室, 绵阳 610000
辛怀璐(1994-),男,河北邯郸人,硕士研究生,从事猪营养研究。E-mail:2047591915@qq.com

收稿日期: 2019-08-21

  网络出版日期: 2020-03-13

基金资助

十三五国家重点研发项目（2016YFD0501204）；四川省重点研发项目（2018NZ0094）；国家生猪产业体系（CARS-35）；四川农业大学铁骑力士7070项目

收起

Effects of Feeding Artificial Milk on Growth Performance, Intestinal Antioxidant Capacity and Barrier Function of Piglets Weaning at 14 Days of Age

  • XIN Huailu ,
  • JIANG Junjie ,
  • CHEN Daiwen ,
  • HE Jian ,
  • LI Yuanfeng ,
  • YU Bing ,
  • HE Jun ,
  • HUANG Zhiqing ,
  • MAO Xiangbing ,
  • YU Jie ,
  • LUO Yuheng ,
  • LUO Junqiu ,
  • ZHENG Ping
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  • 1. Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China;
    2. College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;
    3. Feng Guang De Laboratory of Tols Tie Qi Li Shi Group, Mianyang 610000, China

Received date: 2019-08-21

  Online published: 2020-03-13

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摘要

本试验旨在探究饲喂人工乳对14日龄断奶仔猪生长性能、肠道抗氧化能力及屏障功能的影响。选取14窝共112头平均体重为（4.79±0.56）kg的14日龄"长×大"二元杂交仔猪，随机分为21日龄断奶组和14日龄断奶组，每组7个重复，每个重复8头仔猪。21日龄断奶组仔猪14~21日龄时由原圈母猪饲喂母乳，14日龄断奶组仔猪断奶后饲喂人工乳至21日龄，2组均于22日龄时开始饲喂相同的教槽料，试验期14 d。结果表明：与21日龄断奶相比，1）14日龄断奶极显著降低仔猪15~21日龄时的平均日增重（ADG）（P<0.01），显著提高22~28日龄时的平均日采食量（ADFI）（P<0.05），显著降低21日龄体重和腹泻率（P<0.05）；2）14日龄断奶极显著降低仔猪的空肠隐窝深度（P<0.01），极显著提高空肠绒毛高度/隐窝深度（P<0.01）；3）14日龄断奶极显著提高仔猪回肠NLR家族Pyrin域蛋白3（NLRP3）的mRNA相对表达量（P<0.01），显著降低空肠黏膜过氧化氢酶（CAT）活性（P<0.05）。综上所述，与21日龄断奶相比，人工乳饲喂14日龄断奶仔猪降低28日龄时仔猪的体重、空肠抗氧化能力和屏障功能，但对仔猪28日龄时的肠道形态有改善作用，并降低其腹泻率。

关键词： 人工乳; 断奶日龄; 仔猪; 生长性能; 肠道健康

本文引用格式

辛怀璐 , 蒋俊劼 , 陈代文 , 何健 , 李元凤 , 余冰 , 何军 , 黄志清 , 毛湘冰 , 虞洁 , 罗玉衡 , 罗钧秋 , 郑萍 . 饲喂人工乳对14日龄断奶仔猪生长性能、肠道抗氧化能力及屏障功能的影响[J]. 动物营养学报, 2020 , 32(3) : 1109 -1117 . DOI: 10.3969/j.issn.1006-267x.2020.03.017

Abstract

This experiment was conducted to investigate the effects of feeding artificial milk on growth performance, intestinal antioxidant capacity and barrier function of piglets weaning at 14 days of age. One hundred and twelve "Landrace×Yorkshire" crossbred piglets at 14 days of age with average body weight of (4.79±0.56) kg from 14 litters were randomly assigned to weaning at 21 days of age group and weaning at 14 days of age group, with 7 replicates per group and 8 piglets per replicate. Piglets in weaning at 21 days of age group were fed breast milk by original sows from 14 to 21 days of age, and the others in weaning at 14 days of age group were fed artificial milk after weaning until 21 days of age. Piglets in two groups were fed same creep feed from 22 days of age, and the experiment lasted for 14 days. The results showed as follows:compared with weaning at 21 days of age, 1) weaning at 14 days of age extremely significantly decreased average daily gain (ADG) of piglets from 15 to 21 days of age (P<0.01), significantly increased average daily feed intake (ADFI) from 22 to 28 days of age (P<0.05), and significantly decreased body weight at 21 days of age and diarrhea rate (P<0.05). 2) Weaning at 14 days of age extremely significantly decreased jejunal crypt depth of piglets (P<0.01), and extremely significantly increased villus height to crypt depth ratio (P<0.01). 3) Weaning at 14 days of age extremely significantly increased mRNA relative expression level of ileal NACHT, LRR and PYD domains-containing protein 3 (NLRP3) of piglets (P<0.01), and significantly decreased jejunal mucosa catalase (CAT) activity (P<0.05). In summary, compared with weaning at 21 days of age, weaning at 14 days of age and feeding artificial milk decrease body weight at 28 days of age, jejunal antioxidant capacity and barrier function of piglets, but improve intestinal morphology and reduce diarrhea rate of piglets at 28 days of age.

Key words： artificial milk; weaning day; piglets; growth performance; intestinal health

参考文献

[1] 王恬.仔猪断奶应激及营养调控措施的应用[J].畜牧与兽医,2009,41(5):1-4.
[2] 张苗苗,李晓季.仔猪超早断奶与正常断奶的对比试验[J].中国畜禽种业,2012,8(8):56-58.
[3] 雷小文,苏州,钟云平,等.浅谈利用早期断奶技术控制猪场传染病[J].江西畜牧兽医杂志,2011(6):26-27.
[4] CAMPBELL J M,CRENSHAW J D,POLO J.The biological stress of early weaned piglets[J].Journal of Animal Science and Biotechnology,2013,4:19.
[5] MCLAMB B L,GIBSON A J,OVERMAN E L,et al.Early weaning stress in pigs impairs innate mucosal immune responses to enterotoxigenic E. coli challenge and exacerbates intestinal injury and clinical disease[J].PLoS One,2013,8(4):e59838.
[6] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2010,298(3):G352-G363.
[7] HODGE R W.Efficiency of food conversion and body composition of the preruminant lamb and the young pig[J].British Journal of Nutrition,1974,32(1):113-126.  
[8] ZHENG P,SONG Y,TIAN Y H,et al.Dietary arginine supplementation affects intestinal function by enhancing antioxidant capacity of a nitric oxide-independent pathway in low-birth-weight piglets[J].The Journal of Nutrition,2018,148(11):1751-1759.  
[9] REALE T A.Supplemental liquid diets and feed flavours for young pigs[D].Master's Thesis.Melbourne:The University of Melbourne,1987.
[10] DUNSHEA F R,KERTON D J,EASON P J,et al.Supplemental skim milk before and after weaning improves growth performance of pigs[J].Australian Journal of Agricultural Research,1999,50(7):1165-1170.  
[11] KIM J H,HEO K N,ODLE J,et al.Liquid diets accelerate the growth of early-weaned pigs and the effects are maintained to market weight[J].Journal of Animal Science,2001,79(2):427-434.  
[12] HEO K N,ODLE J,OLIVER W,et al.Effects of milk replacer and ambient temperature on growth performance of 14-day-old early-weaned pigs[J].Asian-Australasian Journal of Animal Sciences,1999,12(6):908-913.  
[13] 田一航,陈代文,余冰,等.低出生重对仔猪免疫功能的影响及精氨酸的营养效应[J].动物营养学报,2018,30(12):125-136.
[14] JIANG J J,CHEN D W,YU B,et al.Improvement of growth performance and parameters of intestinal function in liquid fed early weanling pigs[J].Journal of Animal Science,2019,97:2725-238.
[15] 周芬,冯培刚,张莉莉,等.断奶日龄对仔猪生产性能和消化酶活性的影响[J].畜牧与兽医,2009(6):31-33.
[16] 郑心力,魏立民,晁哲,等.断奶日龄对临高仔猪生产性能的影响[J].广东农业科学,2013,40(1):114-115.
[17] DUNSHEA F R,KERTON D K,CRANWELL P D,et al.Interactions between weaning age,weaning weight,sex,and enzyme supplementation on growth performance of pigs[J].Australian Journal of Agricultural Research,2002,53(8):939-945.  
[18] DE VOS M,HUYGELEN V,WILLEMEN S,et al.Artificial rearing of piglets:effects on small intestinal morphology and digestion capacity[J].Livestock Science,2014,159:165-173.
[19] HU C H,XIAO K,LUAN Z S,et al.Early weaning increases intestinal permeability,alters expression of cytokine and tight junction proteins,and activates mitogen-activated protein kinases in pigs[J].Journal of Animal Science,2013,91(3):1094-1101.  
[20] MILLER B G,JAMES P S,SMITH M W,et al.Effect of weaning on the capacity of pig intestinal villi to digest and absorb nutrients[J].The Journal of Agricultural Science,1986,107(3):579-590.  
[21] MIR H,MEENA A S,CHAUDHRY K K,et al.Occludin deficiency promotes ethanol-induced disruption of colonic epithelial junctions,gut barrier dysfunction and liver damage in mice[J].Biochimica et Biophysica Acta (BBA):General Subjects,2016,1860(4):765-774.  
[22] 张影超,王希彪,崔世泉,等.肠上皮紧密连接蛋白mRNA的表达与仔猪断奶腹泻关系的研究[J].中国畜牧兽医,2014,41(9):221-224.
[23] WEILER F,MARBE T,SCHEPPACH W,et al.Influence of protein kinase C on transcription of the tight junction elements ZO-1 and occludin[J].Journal of Cellular Physiology,2005,204(1):83-86.  
[24] PEREZ-LOPEZ A,BEHNSEN J,NUCCIO S P,et al.Mucosal immunity to pathogenic intestinal bacteria[J].Nature Reviews Immunology,2016,16(3):135-148.  
[25] RATHINAM V A K,VANAJA S K,FITZGERALD K A.Regulation of inflammasome signaling[J].Nature Immunology,2012,13(4):333-342.  
[26] COLSON V,ORGEUR P,FOURY A,et al.Consequences of weaning piglets at 21 and 28 days on growth,behaviour and hormonal responses[J].Applied Animal Behaviour Science,2006,98(1/2):70-88.
[27] WOROBEC E K,DUNCAN I J H,WIDOWSKI T M.The effects of weaning at 7,14 and 28 days on piglet behaviour[J].Applied Animal Behaviour Science,1999,62(2/3):173-182.
[28] YIN J,WU M M,XIAO H,et al.Development of an antioxidant system after early weaning in piglets[J].Journal of Animal Science,2014,92(2):612-619.  
[29] 徐建雄.不同断奶日龄仔猪的氧化应激损伤及其机理研究[D].博士学位论文.南京:南京农业大学,2014.
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