猪营养与饲料 SWINE NUTRITION AND FEED

梅山猪和杜长大新生仔猪肝脏脂代谢功能差异比较

  • 张永胜 ,
  • 喻礼怀 ,
  • 毛俊舟 ,
  • 彭众 ,
  • 仲召鑫 ,
  • 汪天龙 ,
  • 董丽
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  • 扬州大学动物科学与技术学院, 扬州 225009
张永胜(1994-),男,山东东营人,硕士研究生,从事仔猪的断奶应激及营养调控研究。E-mail:1215520238@qq.com

收稿日期: 2019-09-17

  网络出版日期: 2020-03-13

Difference Comparison of Liver Lipid Metabolism between Meishan and Duroc×Landrace×Yorkshire Newborn Piglets

  • ZHANG Yongsheng ,
  • YU Lihuai ,
  • MAO Junzhou ,
  • PENG Zhong ,
  • ZHONG Zhaoxin ,
  • WANG Tianlong ,
  • DONG Li
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2019-09-17

  Online published: 2020-03-13

摘要

本试验旨在比较不同品种新生仔猪肝脏脂代谢功能的差异。选取同胎次新生梅山仔猪和杜长大仔猪各6头,不喂食母乳,出生6 h内颈动脉放血致死,屠宰取其样品。结果表明:1)新生梅山仔猪血清中甘油三酯(TG)含量极显著高于新生杜长大仔猪(P<0.01),2组新生仔猪血清中总胆固醇(TC)、低密度脂蛋白(LDL)含量均没有显著差异(P>0.05)。2)新生杜长大仔猪肝脏指数极显著高于新生梅山仔猪(P<0.01),肝脏细胞面积及粒径均显著低于新生梅山仔猪(P<0.05)。3)新生梅山仔猪肝脏中TC、TG、高密度脂蛋白胆固醇(HDL-C)含量均极显著高于新生杜长大仔猪(P<0.01),肝脏中脂蛋白脂酶(LPL)活性显著低于新生杜长大仔猪(P<0.05)。4)新生梅山仔猪肝脏中LPL mRNA表达水平极显著低于新生杜长大仔猪(P<0.01),脂肪酸合成酶(FAS)mRNA表达水平极显著高于新生杜长大仔猪(P<0.01)。综上所述,新生梅山仔猪肝脏脂质合成代谢功能强于新生杜长大仔猪,这与两者肝脏脂质合成及分解代谢基因表达差异有关。

本文引用格式

张永胜 , 喻礼怀 , 毛俊舟 , 彭众 , 仲召鑫 , 汪天龙 , 董丽 . 梅山猪和杜长大新生仔猪肝脏脂代谢功能差异比较[J]. 动物营养学报, 2020 , 32(3) : 1143 -1150 . DOI: 10.3969/j.issn.1006-267x.2020.03.021

Abstract

This experiment aimed to compare the differences in liver lipid metabolism between newborn piglets of different breeds. Every six Meishan and Duroc×Landrace×Yorkshire (DLY) piglets with the same parity were selected without feeding breast milk and were slaughtered by internal carotid artery bleeding in 6 h after birth for taking samples. The results showed as follows:1) the serum triglyceride (TG) content of newborn Meishan piglets was extremely significantly higher than that of newborn DLY piglets (P<0.01). The serum contents of total cholesterol (TC) and low density lipoprotein (LDL) of the two breeds of newborn piglets were not significantly different (P>0.05). 2) The liver index of newborn DLY piglets was extremely significantly higher than that of newborn Meishan piglets (P<0.01), and the liver cell area and particle size of newborn DLY piglets were significantly higher than that of newborn Meishan piglets (P<0.05). 3) The contents of total TC, TG and high density lipoprotein cholesterol (HDL-C) in liver of newborn Meishan piglets were extremely significantly higher than that of newborn DLY piglets (P<0.01), and the liver lipoprotein lipase (LPL) activity of newborn Meishan piglets was significantly lower than that of newborn DLY piglets (P<0.05). 4) The liver LPL mRNA expression level of newborn Meishan piglets was extremely significantly lower than that of newborn DLY piglets (P<0.01), and the liver fatty acid synthase (FAS) mRNA expression level of newborn Meishan piglets was extremely significantly higher than that of newborn DLY piglets (P<0.01). In summary, the liver lipid anabolic function of the newborn Meishan piglets is stronger than that of newborn DLY piglets, which is related to the differences in liver lipid synthesis and catabolism gene expression between them.

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