猪营养与饲料 SWINE NUTRITION AND FEED

山竹醇对生长育肥猪生长性能、肌肉抗氧化能力和肌纤维类型组成的影响

  • 邵亚飞 ,
  • 贺琼玉 ,
  • 姚卫磊 ,
  • 王同心 ,
  • 夏军 ,
  • 黄飞若
展开
  • 华中农业大学动物科学技术学院, 武汉 430070
邵亚飞(1994-),男,河南商丘人,硕士研究生,动物营养与饲料科学专业。E-mail:ya1506443020@qq.com

收稿日期: 2019-09-05

  网络出版日期: 2020-03-13

基金资助

国家重点研发计划(2018YFD0500600);湖北省青年科学基金(2018CFA071)

Effects of Garcinol on Growth Performance, Muscle Antioxidant Capacity and Muscle Fiber Type of Growing-Finishing Pigs

  • SHAO Yafei ,
  • HE Qiongyu ,
  • YAO Weilei ,
  • WANG Tongxin ,
  • XIA Jun ,
  • HUANG Feiruo
Expand
  • College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2019-09-05

  Online published: 2020-03-13

摘要

本试验旨在研究饲粮中添加山竹醇对生长育肥猪生长性能、肌肉抗氧化能力和肌纤维类型组成的影响。试验选取80日龄左右、健康状况良好且体重[(35.50±1.50)kg]相近的"杜×长×大"三元杂交公猪48头,随机分为4组,每组12个重复,每个重复1头猪。试验期90 d,试验期间,对照组全程饲喂基础饲粮,试验Ⅰ、Ⅱ、Ⅲ组分别在屠宰前30、60、90 d持续饲喂在基础饲粮中添加400 mg/kg山竹醇的试验饲粮。试验结束时,每个重复随机选择6头试验猪进行屠宰,取其背最长肌样品,检测其抗氧化指标、肌纤维类型组成、过氧化物酶体增殖物激活受体γ辅激活因子-1α(PGC-1α)与p300活性以及肌纤维类型和肌肉生长相关基因的mRNA表达量。结果显示:1)与对照组相比,各试验组生长育肥猪全程平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)均无显著影响(P>0.05)。2)与对照组相比,生长育肥猪屠宰前60和90 d饲喂添加400 mg/kg山竹醇的饲粮均可以显著提高背最长肌中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性(P<0.05),显著降低背最长肌中丙二醛(MDA)含量(P<0.05)。3)与对照组相比,生长育肥猪屠宰前60和90 d饲喂添加400 mg/kg山竹醇的饲粮均可以显著提高背最长肌中Ⅰ型肌纤维的比例(P<0.05),显著降低背最长肌中Ⅱ型肌纤维的比例(P<0.05)。4)各组生长育肥猪背最长肌中肌球蛋白重链(MyHC)-Ⅱx的mRNA表达量无显著差异(P>0.05);与对照组相比,生长育肥猪屠宰前60和90 d饲喂添加400 mg/kg山竹醇的饲粮均可以显著提高背最长肌中MyHC-Ⅰ和MyHC-Ⅱa的mRNA表达量(P<0.05),显著降低背最长肌中MyHC-Ⅱb的mRNA表达量(P<0.05)。5)与对照组相比,生长育肥猪屠宰前60和90 d饲喂添加400 mg/kg山竹醇的饲粮均可以显著降低背最长肌中p300活性(P<0.05),显著提高背最长肌中PGC-1α活性(P<0.05)。综合以上结果得出,生长育肥猪屠宰前60或90 d饲喂添加400 mg/kg山竹醇的饲粮可以提高肌肉的抗氧化能力,增加背最长肌中Ⅰ型肌纤维的比例,且具有生理阶段效应。

本文引用格式

邵亚飞 , 贺琼玉 , 姚卫磊 , 王同心 , 夏军 , 黄飞若 . 山竹醇对生长育肥猪生长性能、肌肉抗氧化能力和肌纤维类型组成的影响[J]. 动物营养学报, 2020 , 32(3) : 1151 -1160 . DOI: 10.3969/j.issn.1006-267x.2020.03.022

Abstract

This study was aimed to investigate the effects of dietary garcinol on growth performance, muscle antioxidant capacity and muscle fiber type of growing-finishing pigs. Forty-eight Duroc×Large White×Landrace ternary hybrid boars with healthy condition and similar body weight[(35.50±1.50) kg] were selected and randomly divided into 4 groups with 12 replicates per group and 1 pig per replicate. The trial period was 90 days. During the experimental period, the pigs in control group were fed a basal diet throughout the whole process, while those in trial groups Ⅰ, Ⅱ, and Ⅲ were continuously fed the basal diet supplemented 400 mg/kg garcinol at 30, 60 and 90 days before slaughter, respectively. At the end of the experiment, six pigs were randomly selected from each group for slaughter. The samples of longissimus dorsi were collected and antioxidant indexes, the activities of p300 and peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), muscle fiber type composition, and the mRNA expression levels of muscle fiber type and muscle growth related genes were measured. The results showed as follows:1) compared with the control group, no significant differences were observed in average body weight (ADG), average daily feed intake (ADFI) and feed/gain (F/G) during the whole process of growing-finishing pigs in three trial groups (P>0.05). 2) Compared with the control group, diet contained 400 mg/kg garcinol feeding for 60 and 90 days before slaughter significantly increased the activities of total superoxide dismutase (T-SOD), glutathion peroxidase (GSH-Px) and catalase (CAT) in longissimus dorsi (P<0.05), and significantly decreased the malondialdehyde (MDA) content in longissimus dorsi (P<0.05). 3) Compared with the control group, diet contained 400 mg/kg garcinol feeding for 60 and 90 days before slaughter significantly increased the proportion of Ⅰ type muscle fiber in longissimus dorsi (P<0.05), and significantly decreased the proportion of Ⅱ type muscle fiber in longissimus dorsi (P<0.05). 4) No significant differences were observed in the mRNA expression level of myosin heavy chain (MyHC)-Ⅱx gene in longissimus dorsi among groups (P>0.05). Compared with the control group, diet contained 400 mg/kg garcinol feeding for 60 and 90 days before slaughter significantly increased the mRNA expression levels of MyHC-Ⅰ and MyHC-Ⅱa genes in longissimus dorsi (P<0.05), and significantly decreased the mRNA expression level of MyHC-Ⅱb gene in longissimus dorsi (P<0.05). 5) Compared with the control group, diet contained 400 mg/kg garcinol feeding for 60 and 90 days before slaughter significantly decreased the activity of p300 in longissimus dorsi (P<0.05), and significantly increased the activity of PGC-1α (P<0.05). Above all, these results indicate that diet contained 400 mg/kg garcinol feeding for 60 or 90 days before slaughter can improve the antioxidant capacity of muscle, increase the proportion of Ⅰ type muscle fiber in the longissimus dorsi of growing-finishing pigs, and have a physiological stage effect.

参考文献

[1] 刘培峰,冯佳炜,段晓雪,等.肌纤维类型及其转化对畜禽肉品质的影响[J].中国饲料,2015(16):16-19.
[2] KIM N K,LIM J H,SONG M J,et al.Comparisons of longissimus muscle metabolic enzymes and muscle fiber types in Korean and Western pig breeds[J].Meat Science,2008,78(4):455-460.  
[3] 王继英,王怀中,张印.猪肌纤维特性的研究进展[J].养猪,2004(3):46-48.
[4] JOO S T,KIM G D,HWANG Y H,et al.Control of fresh meat quality through manipulation of muscle fiber characteristics[J].Meat Science,2013,95(4):828-836.  
[5] ZHAO R Q,YANG X J,XU Q F,et al.Expression of GHR and PGC-lα in association with changes of MyHC isoform types in longissimus muscle of Erhualian and Large White pigs (Sus scrofa) during postnatal growth[J].Animal Science,2004,79(2):203-211.  
[6] PETTE D,STARON R S.Myosin isoforms,muscle fiber types,and transitions[J].Microscopy Research and Technique,2000,50(6):500-509.  
[7] LIN J D,WU H,TARR P T,et al.Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibres[J].Nature,2002,418(6899):797-801.  
[8] SCHULER M,ALI F,CHAMBON C,et al.PGC1α expression is controlled in skeletal muscles by PPARβ,whose ablation results in fiber-type switching,obesity,and type 2 diabetes[J].Cell Metabolism,2006,4(5):407-414.  
[9] CHALKIADAKI A,IGARASHI M,NASAMU A S,et al.Muscle-specific SIRT1 gain-of-function increases slow-twitch fibers and ameliorates pathophysiology in a mouse model of duchenne muscular dystrophy[J].PLoS Genetics,2014,10(7):e1004490.
[10] HANDSCHIN C,CHIN S,LI P,et al.Skeletal muscle fiber-type switching,exercise intolerance,and myopathy in PGC-1α muscle-specific knock-out animals[J].The Journal of Biological Chemistry,2007,282(41):30014-30021.  
[11] WALLBERG A E,YAMAMURA S,MALIK S,et al.Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1α[J].Molecular Cell,2003,12(5):1137-1149.  
[12] SAADAT N,GUPTA S V.Potential role of garcinol as an anticancer agent[J].Journal of Oncology,2012,2012:647206.
[13] PANDA V,ASHAR H,SRINATH S.Antioxidant and hepatoprotective effect of Garcinia indica fruit rind in ethanolinduced hepatic damage in rodents[J].Interdisciplinary Toxicology,2012,5(4):207-213.  
[14] KIRANA H,SRINIVASAN B P.Aqueous extract of Garcinia indica choisy restores glutathione in type 2 diabetic rats[J].Journal of Young Pharmacists,2010,2(3):265-268.  
[15] DU T F,NAGAI Y,XIAO Y,et al.Lysosome-dependent p300/FOXP3 degradation and limits Treg cell functions and enhances targeted therapy against cancers[J].Experimental and Molecular Pathology,2013,95(1):38-45.  
[16] HO C T,PAN M H,HO Y S.The molecular targets of garcinol confer antitumor effects[M]//HO C T,MUSSINAN C,SHAHIDIF,et al.Nutrition,functional and sensory properties of foods.Cambridge:RSC Publishing,2013:251-255.
[17] BEHERA A K,SWAMY M M,NATESH N,et al.Garcinol and its role in chronic diseases[M]//GUPTA S C,PRASAD S,AGGARWAL B B.Anti-inflammatory nutraceuticals and chronic diseases.Cham:Springer,2016:435-452.
[18] 章涛,张潜,胡锡阶,等.改良骨骼肌肌纤维分型ATP酶染色方法[J].西安交通大学学报(医学版),2009,30(2):254-256.
[19] 万伟.mTORC1调控乙酰转移酶p300活性的机制及功能研究[D].博士学位论文.杭州:浙江大学,2017.
[20] LEE P S,TENG C Y,KALYANAM N,et al.Garcinol reduces obesity in high-fat-diet-fed mice by modulating gut microbiota composition[J].Molecular Nutrition & Food Research,2019,63(2):1800390.
[21] 杜金芳.猪CuZnSOD基因的克隆、mRNA表达及其对肉质抗氧化性能的影响[D].硕士学位论文.泰安:山东农业大学,2011.
[22] NEGI P S,JAYAPRAKASHA G K.Control of foodborne pathogenic and spoilage bacteria by garcinol and Garcinia indica extracts,and their antioxidant activity[J].Journal of Food Science,2006,69(3):FMS61-FMS65.
[23] PRIYADARSINI K I,MAITY D K,NAIK G H,et al.Role of phenolic O-H and methylene hydrogen on the free radical reactions and antioxidant activity of curcumin[J].Free Radical Biology and Medicine,2003,35(5):475-484.  
[24] 田刚,黄琳惠,宋晓华,等.壳寡糖对氧化应激仔猪生长性能、抗氧化能力及空肠养分消化和转运能力的影响[J].动物营养学报,2018,30(7):2652-2661.
[25] 田春庄.鱼油上调断奶仔猪肌纤维类型及相关基因表达促进肌肉生长的研究[D].硕士学位论文.武汉:华中农业大学,2008.
[26] 韩丽,潘杰,解培峰,等.低聚木糖对生长肥育猪血浆生化参数、肌肉氨基酸含量和肌纤维类型组成的影响[J].动物营养学报,2018,30(5):1880-1886.
[27] ZHANG C,LUO J Q,YU B,et al.Dietary resveratrol supplementation improves meat quality of finishing pigs through changing muscle fiber characteristics and antioxidative status[J].Meat Science,2015,102:15-21.
[28] 李飞,许厚强,陈伟,等.关岭牛MyoD Ⅰ基因启动子报告质粒的构建及活性验证[J].基因组学与应用生物学,2013,32(4):459-466.
[29] 项露颉.生肌决定因子MyoD在不同品种肉羊肌肉中表达差异的研究[D].硕士学位论文.长春:吉林农业大学,2017.
[30] 赵地.乳酸脱氢酶A和醛脱氢酶1A1的乙酰化在肿瘤发生发展中的作用及其分子机制[D].博士学位论文.上海:复旦大学,2014.
[31] 闫春雷,黄欣,苏乐群.PGC-1α的转录调节和翻译后修饰[J].中国生物化学与分子生物学报,2015,31(1):12-19.
[32] LJUBICIC V,BURT M,LUNDE J A,et al.Resveratrol induces expression of the slow,oxidative phenotype in mdx mouse muscle together with enhanced activity of the SIRT1-PGC-1α axis[J].American Journal of Physiology:Cell Physiology,2014,307(1):C66-C82.
[33] CHAU M D L,GAO J P,YANG Q,et al.Fibroblast growth factor 21 regulates energy metabolism by activating the AMPK-SIRT1-PGC-1α pathway[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(28):12553-12558.  
文章导航

/