动物营养学报 >

2020 , Vol. 32 >Issue 3: 1293 - 1304

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.037

水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

饲料蛋氨酸水平对松浦镜鲤生长性能、肌肉品质及肌肉合成通路相关基因表达的影响

  • 程龙 ,
  • 王连生 ,
  • 徐奇友
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  • 1. 上海海洋大学水产与生命学院, 上海 201306;
    2. 中国水产科学研究院黑龙江水产研究所, 哈尔滨 150070;
    3. 湖州师范学院生命科学学院, 浙江省水生生物资源养护与开发技术研究重点实验室, 湖州 313000
程龙(1992-),男,山东济宁人,硕士研究生,研究方向为水生动物营养。E-mail:cheng_long93@163.com

收稿日期: 2019-09-23

  网络出版日期: 2020-03-13

基金资助

国家自然科学基金（31972800）；国家大宗淡水鱼产业技术体系（CARS-46）

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Effects of Dietary Methionine Level on Growth Performance, Muscle Quality and Muscle Synthesis Pathway Related Gene Expression of Songpu Mirror Carp

  • CHENG Long ,
  • WANG Liansheng ,
  • XU Qiyou
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  • 1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
    2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
    3. Zhejiang Provincial Key Laboratory of Aquatic Bioresource Conservation and Development Technology, College of Life Sciences, Huzhou Normal University, Huzhou 313000, China

Received date: 2019-09-23

  Online published: 2020-03-13

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摘要

本试验旨在研究饲料蛋氨酸（Met）水平对松浦镜鲤生长性能、肌肉品质及肌肉合成通路相关基因表达的影响。选取540尾初始体质量为（24.42±0.19）g的松浦镜鲤幼鱼，随机分为6组，每组3个重复，每个重复30尾鱼。各组分别饲喂Met水平分别为0.56%（对照）、0.66%、0.75%、0.83%、0.92%和1.01%的等氮等能饲料。试验期8周。结果表明：1）0.83%、0.92%和1.01%组的增重率、特定生长率、蛋白质效率及肥满度均显著高于对照组（P<0.05），饲料系数显著低于对照组（P<0.05）。2）0.83%、0.92%和1.01%组的肌肉水分含量显著低于对照组（P<0.05），0.83%、0.92%和1.01%组的肌肉粗蛋白质含量显著高于对照组（P<0.05），0.92%和1.01%组的肌肉超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性均显著低于对照组（P<0.05）。3）0.83%和0.92%组的快肌和慢肌胰岛素样生长因子-Ⅰ（IGF-Ⅰ）基因表达量显著高于对照组（P<0.05），0.83%、0.92%和1.01%组的快肌和慢肌雷帕霉素靶蛋白（mTOR）基因表达量显著高于对照组（P<0.05），0.66%、0.75%、0.83%、0.92%和1.01%组的慢肌丝氨酸/苏氨酸蛋白激酶11（STK11）基因表达量均显著高于对照组（P<0.05），0.92%和1.01%组的快肌磷脂酰肌醇-3激酶调节亚基3（PIK3R3）基因表达量显著高于对照组（P<0.05），0.66%、0.75%、0.83%、0.92%和1.01%组的快肌和慢肌肌球蛋白重链（MHC）基因表达量均显著高于对照组（P<0.05），0.83%、0.92%和1.01%组快肌和慢肌核因子E2相关因子2（Nrf2）基因表达量均显著高于对照组（P<0.05）。由此可见，饲料添加Met可以促进鲤鱼生长，提高鲤鱼肌肉品质并促进肌肉合成相关基因的表达。当饲料半胱氨酸水平为0.64%时，以特定生长率和蛋白质效率为评价指标，通过折线模型分析得到松浦镜鲤幼鱼Met需要量为0.90%。

关键词： 蛋氨酸; 松浦镜鲤; 肌肉; 品质; 合成通路; 基因表达

本文引用格式

程龙 , 王连生 , 徐奇友 . 饲料蛋氨酸水平对松浦镜鲤生长性能、肌肉品质及肌肉合成通路相关基因表达的影响[J]. 动物营养学报, 2020 , 32(3) : 1293 -1304 . DOI: 10.3969/j.issn.1006-267x.2020.03.037

Abstract

This experiment was conducted to study the effects of dietary methionine (Met) level on growth performance, muscle quality and muscle synthesis pathway related gene expression of Songpu mirror carp. A total of 540 juvenile Songpu mirror carp with the initial body weight of (24.42±0.19) g were randomly divided into 6 groups with 3 replicates per group and 30 fish per replicate. Fish in 6 groups were fed the isonitrogenous and isoenergetic diets with the Met levels of 0.56% (control), 0.66%, 0.75%, 0.83%, 0.92% and 1.01%, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) the weight gain rate, specific growth rate, protein efficiency ratio and condition factor of 0.83%, 0.92% and 1.01% groups were significantly higher than those of the control group (P<0.05), and the feed conversion ratio was significantly lower than that of the control group (P<0.05). 2) The muscle moisture content of 0.83%, 0.92% and 1.01% groups was significantly lower than that of the control group (P<0.05), the muscle crude protein content of 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in muscle of 0.92% and 1.01% groups were significantly lower than those of the control group (P<0.05). 3) The gene expression of insulin-like growth factor (IGF-Ⅰ) in fast-twitch muscle and slow-twitch muscle of 0.83% and 0.92% groups was significantly higher than that of the control group (P<0.05), the gene expression of mammalian target of rapamycin (mTOR) in fast-twitch muscle and slow-twitch muscle of 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), the gene expression of serine/threonine protein kinase 11 (STK11) in slow-twitch muscle of 0.66%, 0.75%, 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), the gene expression of phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) in fast-twitch muscle of 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), the gene expression of myosin heavy chain (MHC) in fast-twitch muscle and slow-twitch muscle of 0.66%, 0.75%, 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), and the gene expression of nuclear factor E2-related factor 2 (Nrf2) in fast-twitch muscle and slow-twitch muscle of 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05). In conclusion, the addition of Met in diet can improve the growth and muscle quality of carp, and promote expression of muscle synthesis pathway related gene. When dietary cystine level is 0.64%, based on specific growth rate and protein efficiency ratio, using the broken-line model analysis indicate that the Met requirement of juvenile Songpu mirror carp is 0.90%.

Key words： methionine; Songpu mirror carp; muscle; quality; synthesis pathway; gene expression

参考文献

[1] MICHELATO M,ZAMINHAN M,BOSCOLO W R,et al.Dietary histidine requirement of Nile tilapia juveniles based on growth performance,expression of muscle-growth-related genes and haematological responses[J].Aquaculture,2016,467:63-70.
[2] OVERTURF K,BARROWS F T,HARDY R W,et al.Energy composition of diet affects muscle fiber recruitment,body composition,and growth trajectory in rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2016,457:1-14.
[3] KASPER C S,WHITE M R,BROWN P B.Choline is required by tilapia when methionine is not in excess[J].The Journal of Nutrition,2000,130(2):238-242.  
[4] GOLDSPINK G.5-postembryonic growth and differentiation of striated muscle[M]//BOURNE G H.The structure and function of muscle.New York:Elsevier,1972,179:179-236.
[5] ROWLERSON A,VEGGETTI A.5-cellular mechanisms of post-embryonic muscle growth in aquaculture species[J].Fish Physiology,2001,18:103-140.
[6] LUO Z,LIU Y J,MAI K S,et al.Dietary L-methionine requirement of juvenile grouper Epinephelus coioides at a constant dietary cystine level[J].Aquaculture,2005,249(1/2/3/4):409-418.
[7] GAYLORD T G,BARROWS F T,TEAGUE A M,et al.Supplementation of taurine and methionine to all-plant protein diets for rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2007,269(1/2/3/4):514-524.
[8] CLAY L T A,WANG S Y,WOLTERS W R,et al.Molecular characterization of the insulin-like growth factor-Ⅰ (IGF-Ⅰ) gene in channel catfish (Ictalurus punctatus)[J].Biochimica et Biophysica Acta:Gene Structure and Expression,2005,1731(3):139-148.  
[9] SHAN L L,LI X Q,ZHENG X M,et al.Effects of feed processing and forms of dietary methionine on growth and IGF-1 expression in Jian carp[J].Aquaculture Research,2017,48(1):56-67.  
[10] 徐奇友.鲤鱼营养需要研究进展[J].饲料工业,2019,40(14):1-10.
[11] RESOURCES N.Nutrient requirements of fish and shrimp[J].Nutrient Requirements of Fish & Shrimp,2011,23(10):2-8.
[12] BONE Q.6-locomotor muscle[J].Fish Physiology,1979,7:361-424.
[13] YANG H J,LIU Y J,TIAN L X,et al.Effects of supplemental lysine and methionine on growth performance and body composition for grass carp (Ctenopharyngodon idella)[J].American Journal of Agricultural and Biological Sciences,2010,5(2):222-227.  
[14] SARDAR P,ABID M,RANDHAWA H S,et al.Effect of dietary lysine and methionine supplementation on growth,nutrient utilization,carcass compositions and haemato-biochemical status in Indian major carp,Rohu (Labeo rohita H.) fed soy protein-based diet[J].Aquaculture Nutrition,2009,15(4):33-346.
[15] ABIMORAD E G,FAVERO G C,CASTELLANI D,et al.Dietary supplementation of lysine and/or methionine on performance,nitrogen retention and excretion in pacu Piaractus mesopotamicus reared in cages[J].Aquaculture,2009,295(3/4):266-270.
[16] SCHWARZ F J,KIRCHGESSNER M,DEURINGER U.Studies on the methionine requirement of carp (Cyprinus carpio L.)[J].Aquaculture,1998,161(1/2/3/4):121-129.
[17] TANG L,WANG G X,JIANG J,et al.Effect of methionine on intestinal enzymes activities,microflora and humoral immune of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition,2009,15(5):477-483.  
[18] BELGHIT I,SKIBA-CASSY S,GEURDEN I,et al.Dietary methionine availability affects the main factors involved in muscle protein turnover in rainbow trout (Oncorhynchus mykiss)[J].British Journal of Nutrition,2014,112(4):493-503.  
[19] CASTILLO J,CODINA M,MART?NEZ M L,et al.Metabolic and mitogenic effects of IGF-Ⅰ and insulin on muscle cells of rainbow trout[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2004,286(5):R935-R941.
[20] ESPE M,VEISETH-KENT E,ZERRAHN J E,et al.Juvenile Atlantic salmon decrease white trunk muscle IGF-1 expression and reduce muscle and plasma free sulphur amino acids when methionine availability is low while liver sulphur metabolites mostly is unaffected by treatment[J].Aquaculture Nutrition,2016,22(4):801-812.  
[21] CASTILLO J,AMMENDRUP-JOHNSEN I,CODINA M,et al.IGF-Ⅰ and insulin receptor signal transduction in trout muscle cells[J].American Journal of Physiology-Regulatory,Integrative and Comparative Physiology,2006,290(6):R1683-R1690.
[22] SNIJDERS T,NEDERVEEN J P,MCKAY B R,et al.Satellite cells in human skeletal muscle plasticity[J].Frontiers in Physiology,2015,6:283.
[23] KIM E.Mechanisms of amino acid sensing in mTOR signaling pathway[J].Nutrition Research and Practice,2009,3(1):64-71.  
[24] DESTEFANO M,JACINTO E.Regulation of insulin receptor substrate-1 by mTORC2(mammalian target of rapamycin complex 2)[J].Biochemical Society Transactions,2013,41(4):896-901.  
[25] GLASS D J.PI3 kinase regulation of skeletal muscle hypertrophy and atrophy[M]//ROMMEL C,VANHAESEBROECK B,VOGT P.Phosphoinositide 3-kinase in health and disease.Berlin,Heidelberg:Springer,2010,346:267.
[26] AZIZI S,ALI NEMATOLLAHI M,AMIRI B M,et al.IGF-Ⅰ and IGF-Ⅱ effects on local IGF system and signaling pathways in gilthead sea bream (Sparus aurata) cultured myocytes[J].General and Comparative Endocrinology,2016,232:7-16.
[27] NANKERVIS L,MATTHEWS S J,APPLEFORD P.Effect of dietary non-protein energy source on growth,nutrient retention and circulating insulin-like growth factor Ⅰ and triiodothyronine levels in juvenile barramundi,Lates calcarifer[J].Aquaculture,2001,191(4):323-335.
[28] DYER A R,BARLOW C G,BRANSDEN M P,et al.Correlation of plasma IGF-Ⅰ concentrations and growth rate in aquacultured finfish:a tool for assessing the potential of new diets[J].Aquaculture,2004,236(1/2/3/4):583-592.
[29] 寇涛,胡志萍,董丽,等.二甲基甘氨酸钠对肉鸡屠宰性能、肉品质及肌肉抗氧化能力的影响[J].食品科学,2015,36(5):179-184.
[30] 周光宏.肉品学[M].北京:中国农业科技出版社,1999:221-262.
[31] 蒋雪樱,张相伦,陆鹏,等.蛋氨酸对肉鸡屠宰性能、肉品质及肌肉抗氧化的影响[J].食品科学,2016,37(21):114-118.
[32] MA Q.Role of Nrf2 in oxidative stress and toxicity[J].Annual Review of Pharmacology and Toxicology,2013,53:401-426.
[33] LUO J B,FENG L,JIANG W D,et al.Physical and flavor characteristics,fatty acid profile,antioxidant status and Nrf2-dependent antioxidant enzyme gene expression changes in young grass carp (Ctenopharyngodon idella) fillets fed dietary valine[J].PLoS One,2017,12(1):e0169270.
[34] DENG Y P,JIANG W D,LIU Y,et al.Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp (Ctenopharyngodon idella)[J].Aquaculture,2016,452:380-387.
[35] XIAO W W,FENG L,KUANG S Y,et al.Lipid peroxidation,protein oxidant and antioxidant status of muscle and serum for juvenile Jian carp (Cyprinus carpio var.Jian) fed grade levels of methionine hydroxy analogue[J].Aquaculture Nutrition,2012,18(1):90-97.  
[36] ELMADA C Z,HUANG W,JIN M,et al.The effect of dietary methionine on growth,antioxidant capacity,innate immune response and disease resistance of juvenile yellow catfish (Pelteobagrus fulvidraco)[J].Aquaculture Nutrition,2016,22(6):1163-1173.  
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