水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

饲料脂肪源对珍珠龙胆石斑鱼生长性能、血清生化指标及肝脏脂肪酸组成、脂肪代谢相关指标的影响

  • 吴美焕 ,
  • 安文强 ,
  • 董晓慧 ,
  • 谭北平 ,
  • 章双 ,
  • 欧光海 ,
  • 苏泳霖 ,
  • 李雨春 ,
  • 梁胜凯
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  • 1. 广东海洋大学水产学院, 湛江 524088;
    2. 广东海洋大学水产动物营养与饲料实验室, 湛江 524088
吴美焕(1997-),女,广东茂名人,本科生,研究方向为水产动物营养与饲料。E-mail:873163708@qq.com

收稿日期: 2019-08-27

  网络出版日期: 2020-03-13

基金资助

国家自然科学基金项目(31972808);广东大学生科技创新培育专项基金(pdjh2019a0228);广东海洋大学"冲一流"省财政专项(231419011);现代农业产业技术体系专项(CARS-47)

Effects of Dietary Lipid Sources on Growth Performance, Serum Biochemical Indexes and Liver Fatty Acids Composition, Lipid Metabolism Related Indexes of Epinephelus lanceolatus×Epinephelus fuscoguttatus

  • WU Meihuan ,
  • AN Wenqiang ,
  • DONG Xiaohui ,
  • TAN Beiping ,
  • ZHANG Shuang ,
  • OU Guanghai ,
  • SU Yonglin ,
  • LI Yuchun ,
  • LIANG Shengkai
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  • 1. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
    2. Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China

Received date: 2019-08-27

  Online published: 2020-03-13

摘要

本试验旨在研究饲料脂肪源对珍珠龙胆石斑鱼生长性能、血清生化指标及肝脏脂肪酸组成、脂肪代谢相关指标的影响。挑选450尾体格健壮、大小均匀、平均体重为(17.67±0.03)g的珍珠龙胆石斑鱼鱼苗,随机分为5组,每组3个重复,每个重复30尾鱼。各组分别投喂以鱼油(FO)、豆油(SO)、亚麻籽油(LO)、菜籽油(RO)、花生油(PO)为脂肪源配制的5种等氮(粗蛋白质约49%)、等脂(粗脂肪约11%)试验饲料,试验期8周。结果表明:1)FO组和LO组的增重率(WGR)、特定生长率(SGR)显著高于PO组(P<0.05)。2)RO组的肝脏C18:1含量最高,显著高于其他各组(P<0.05);SO组的肝脏C18:2n-6含量显著高于除PO组外的其他各组(P<0.05);FO组的肝脏C20:5n-3、C22:6n-3含量显著高于其他各组(P<0.05)。3)PO组的血清甘油三酯(TG)含量显著高于其他各组(P<0.05);FO组的血清超氧化物酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性最高,显著高于其他各组(P<0.05)。4)LO组的肝脏谷草转氨酶(AST)活性显著低于SO组(P<0.05),谷丙转氨酶(ALT)活性显著高于FO组和RO组(P<0.05),总抗氧化能力(T-AOC)显著低于FO组和PO组(P<0.05)。FO组的肝脏脂肪酸合成酶(FAS)活性显著高于除LO组外的其他各组(P<0.05),LO组的肝脏肉碱脂酰转移酶1(CPT-1)活性显著高于PO组(P<0.05)。综上所述,饲料脂肪源对珍珠龙胆石斑鱼的生长性能、血清生化指标及肝脏脂肪酸组成、脂肪代谢相关指标均有一定的影响。FO组和LO组的生长性能相对较好,而SO组和RO组次之,SO、LO、RO可以作为珍珠龙胆石斑鱼饲料中良好的植物油源。

本文引用格式

吴美焕 , 安文强 , 董晓慧 , 谭北平 , 章双 , 欧光海 , 苏泳霖 , 李雨春 , 梁胜凯 . 饲料脂肪源对珍珠龙胆石斑鱼生长性能、血清生化指标及肝脏脂肪酸组成、脂肪代谢相关指标的影响[J]. 动物营养学报, 2020 , 32(3) : 1315 -1326 . DOI: 10.3969/j.issn.1006-267x.2020.03.039

Abstract

This experiment was conducted to investigate the effects of dietary lipid sources on growth performance, serum biochemical indexes and liver fatty acids composition, lipid metabolism related indexes of Epinephelus lanceolatus×Epinephelus fuscoguttatus. A total of 450 athletic, uniform juvenile Epinephelus lanceolatus×Epinephelus fuscoguttatus with an average body weight of (17.67±0.03) g were randomly divided into 5 groups with 3 replicates per group and 30 fish per replicate. Fish in each group were fed five isonitrogenous (about 49% crude protein) and isolipidic (about 11% crude lipid) experimental diets which were formulated by fish oil (FO), soybean oil (SO), linseed oil (LO), rapeseed oil (RO) and peanut oil (PO) as lipid sources, and the experiment lasted for 8 weeks. The results showed as follows:1) the weight gain rate (WGR) and specific growth rate (SGR) of FO group and LO group were significantly higher than those of PO group (P<0.05). 2) The liver C18:1 content of RO group was the highest, and significantly higher than that of the other groups (P<0.05); the liver C18:2n-6 content of SO group was significantly higher than that of other groups except PO group (P<0.05); and the contents of C20:5n-3 and C22:6n-3 in liver of FO group were significantly higher than those of the other groups (P<0.05). 3) The serum triglycerides (TG) content of PO group was significantly higher than that of the other groups (P<0.05); the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in serum of FO group were the highest, and significantly higher than those of the other groups (P<0.05). 4) The liver aspartate aminotransferase (AST) activity of LO group was significantly lower than that of SO group (P<0.05), and the alanine aminotransferase (ALT) activity was significantly higher than that of FO group and RO group (P<0.05), but the total antioxidant capacity (T-AOC) was significantly lower than that of FO group and PO group (P<0.05). The liver fatty acid synthase (FAS) activity of FO group was significantly higher than that of the other groups except LO group (P<0.05), and the liver carnitine palmitoyltransferase-1 (CPT-1) activity of LO group was significantly higher than that of PO group (P<0.05). To sum up, dietary lipid sources have certain influence on growth performance, serum biochemical indexes and liver fatty acids composition, lipid metabolism related indexes of Epinephelus lanceolatus×Epinephelus fuscoguttatus. The growth performance of FO group and LO group is relatively good, while SO group and RO group take second place, SO, LO and RO can be used as a good vegetable oil source in the diet of Epinephelus lanceolatus×Epinephelus fuscoguttatus.

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