动物营养学报 >

2020 , Vol. 32 >Issue 3: 1327 - 1333

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.040

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

香菇多糖对脂多糖刺激断奶仔猪肌肉组织炎症反应及蛋白质降解相关基因表达的影响

  • 万志成 ,
  • 汪龙梅 ,
  • 张琳 ,
  • 秦琴 ,
  • 王秀英 ,
  • 朱惠玲 ,
  • 刘玉兰 ,
  • 康萍
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  • 武汉轻工大学, 动物营养与饲料科学湖北省重点实验室, 武汉 430023
万志成(1994-),男,湖北应城人,硕士研究生,从事动物营养研究。E-mail:991294573@qq.com

收稿日期: 2019-09-23

  网络出版日期: 2020-03-13

基金资助

武汉市应用基础前沿项目（2018020401011304）

收起

Effects of Lentinan on Expression of Inflammation and Protein Degradation-Related Genes in Muscle of Weaned Piglets Challenged by Lipopolysaccharide

  • WAN Zhicheng ,
  • WANG Longmei ,
  • ZHANG Lin ,
  • QIN Qin ,
  • WANG Xiuying ,
  • ZHU Huiling ,
  • LIU Yulan ,
  • KANG Ping
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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2019-09-23

  Online published: 2020-03-13

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摘要

本试验的目的是探究香菇多糖（LNT）对脂多糖（LPS）刺激断奶仔猪肌肉组织炎症反应及蛋白质降解相关基因表达的影响。试验选取24头体重（7.84±0.21）kg的健康三元杂交断奶仔猪，按照体重近似原则随机分为2组：对照组（12头）和香菇多糖组（12头）。对照组饲喂基础饲粮，香菇多糖组饲喂基础饲粮+0.02%香菇多糖。饲喂28 d后，每组选6头猪腹膜注射100 μg/kg BW的LPS，剩下的6头则等量注射0.9%生理盐水，4 h后将仔猪麻醉、屠宰，取肌肉样品检测Toll样受体4（TLR4）、核苷酸结合寡聚化结构域（NOD）以及丝氨酸/苏氨酸蛋白激酶（Akt）/叉头转录因子（FOXO）信号通路相关基因mRNA表达量。结果显示：1）注射LPS后，仔猪TLR4、骨髓分化因子88（MyD88）、NOD2、受体相互作用丝氨酸/苏氨酸蛋白激酶2（RIPK2）和肿瘤坏死因子-α（TNF-α）在背最长肌和腓肠肌中的mRNA表达量均显著上调（P<0.05），且核转录因子-κB（NF-κB）在腓肠肌中的mRNA表达量显著上调（P<0.05）。而添加香菇多糖后，背最长肌中MyD88、TNF-α和腓肠肌中TLR4、RIPK2、NF-κB的mRNA表达量显著上调（P<0.05）。2）注射LPS后，仔猪背最长肌和腓肠肌中FOXO1和肌肉环指蛋白1（MuRF1）的mRNA表达量显著上调（P<0.05），Akt1的mRNA表达量显著下调（P<0.05）。而添加香菇多糖后，腓肠肌中肌萎缩F-box（MAFbx）的mRNA表达量显著下调（P<0.05）。以上结果表明，在断奶仔猪饲粮中添加0.02%香菇多糖可能在应激急性期通过进一步激活LPS刺激导致的断奶仔猪肌肉组织中的TLR4和NOD信号通路来加强机体免疫力，同时通过调节Akt/FOXO信号通路相关基因表达来减缓肌肉蛋白质的降解。

关键词： 香菇多糖; 脂多糖; 断奶仔猪; 肌肉; TLR4信号通路; NOD信号通路; Akt/FOXO信号通路

本文引用格式

万志成 , 汪龙梅 , 张琳 , 秦琴 , 王秀英 , 朱惠玲 , 刘玉兰 , 康萍 . 香菇多糖对脂多糖刺激断奶仔猪肌肉组织炎症反应及蛋白质降解相关基因表达的影响[J]. 动物营养学报, 2020 , 32(3) : 1327 -1333 . DOI: 10.3969/j.issn.1006-267x.2020.03.040

Abstract

This experiment was conducted to investigate the effects of lentinan (LNT) on the expression of inflammation and protein degradation-related genes in muscle of weaned piglets challenged by lipopolysaccharide (LPS). Twenty-four healthy weaned piglets with the body weight of (7.84±0.21) kg were randomly divided into 2 groups According to the similar body weight principle:control group (n=12) and LNT group (n=12). Weaned piglets in the control group were fed a basal diet, and those in LNT group were fed the basal diet+0.02% LNT. After feeding 28 days, six piglets in each group were selected and injected intraperitoneally with LPS at 100 μg/kg BW, and the others in each group were injected intraperitoneally with 0.9% saline at the same dosage. At 4 h after injection, piglets were sacrificed for muscle samples collection. The muscle samples were used to detected the mRNA expression levels of key genes in Toll-like receptor 4 (TLR4), nucleotide binding oligomerization domain (NOD) and serine-threonine kinase (Akt)/forkhead transcription factor (FOXO) signaling pathways. The results showed as follows:1) after LPS injection, the mRNA expression levels of TLR4, bone marrow differentiation factor 88 (MyD88), NOD2, receptor-interacting serine/threonine protein kinase 2 (RIPK2) and tumor necrosis factor-α (TNF-α) were significantly up-regulated in longissimus dorsi and gastrocnemius muscles (P<0.05), and the mRNA expression level of nuclear factor-kappa B (NF-κB) in gastrocnemius muscle was significantly up-regulated (P<0.05). The mRNA expression levels of MyD88, TNF-α in longissimus dorsi muscle and TLR4, RIPK2 and NF-κB in gastrocnemius muscle were significantly up-regulated after adding lentinan (P<0.05). 2) After LPS injection, the mRNA expression levels of FOXO1 and muscle ring finger protein 1 (MuRF1) in longissimus dorsi and gastrocnemius muscles were significantly up-regulated (P<0.05), while the mRNA expression level of Akt1 was significantly down regulated (P<0.05). When LNT was added, the mRNA expression level of muscle atrophy F-box (MAFbx) in gastrocnemius muscle was significantly down regulated (P<0.05). These results suggest that adding 0.02% LNT to the diet of weaned piglets can enhance immunity by further activating TLR4 and NOD signaling pathways in muscle tissue of weaned piglets induced by LPS stimulation in acute phase of stress, and regulate the expression of Akt/FOXO signaling pathway-related genes, therefore slow down the degradation of muscle protein.

Key words： lentinan; lipopolysaccharide; weaned piglet; muscle; TLR4 signaling pathway; NOD signaling pathway; Akt/FOXO signaling pathway

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