不同品种乳鸽肠道消化酶活性及回肠闭合小环蛋白-1、闭锁蛋白基因表达的差异研究

马晓汶; 田勇; 李国勤; 徐倩倩; 陈晓燕; 陶争荣; 温积辉; 崔世豪; 卢立志; 邹晓庭

doi:10.3969/j.issn.1006-267x.2020.03.041

动物营养学报 >

2020 , Vol. 32 >Issue 3: 1334 - 1343

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.041

分子与细胞营养 MOLECULAR AND CELLULAR NUTRITION

不同品种乳鸽肠道消化酶活性及回肠闭合小环蛋白-1、闭锁蛋白基因表达的差异研究

马晓汶 ,
田勇 ,
李国勤 ,
徐倩倩 ,
陈晓燕 ,
陶争荣 ,
温积辉 ,
崔世豪 ,
卢立志 ,
邹晓庭

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1. 浙江大学动物科学学院, 浙江省饲料与动物营养重点实验室, 农业部(华东)动物营养与饲料重点实验室, 浙江大学动物分子营养学教育部重点实验室, 杭州 310058;
2. 浙江省农业科学院畜牧兽医研究所, 杭州 310021;
3. 平阳县敖峰鸽业有限公司, 温州 325400;
4. 中南百草原集团有限公司, 南太湖精英计划领军型创新团队, 湖州 313300

马晓汶(1994-),女,江苏南通人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:maxiaowen0319@163.com

收稿日期: 2019-09-23

网络出版日期: 2020-03-13

基金资助

浙江省农业（畜禽）新品种选育重大科技专项（2016C02054-16）；浙江省农业科学院地方科技合作项目（WZ2017002）

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Differences of Intestinal Digestive Enzyme Activities and Ileal Zonula Occludens-1 and Occludin Gene Expression of Different Breeds of Pigeon Squabs

MA Xiaowen ,
TIAN Yong ,
LI Guoqin ,
XU Qianqian ,
CHEN Xiaoyan ,
TAO Zhengrong ,
WEN Jihui ,
CUI Shihao ,
LU Lizhi ,
ZOU Xiaoting

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1. College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Key Laboratory of Animal Nutrition and Feed Sciences in East China of Ministry of Agriculture, Key Laboratory of Molecular Animal Nutrition of Ministry of Education, Hangzhou 310058, China;
2. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Pingyang County Aofeng Pigeon Industry Co., Ltd., Wenzhou 325400, China;
4. Zhongnan Bai Grassland Group Co., Ltd., Leading Innovation Team Project of South Taihu Elite Program, Huzhou 313300, China

Received date: 2019-09-23

Online published: 2020-03-13

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摘要

本研究旨在比较不同品种乳鸽肠道消化酶活性及回肠闭合小环蛋白-1（ZO-1）、闭锁蛋白（Occludin）基因表达的差异。试验选取1日龄深王鸽、卡奴鸽和敖峰鸽各120羽，3个品种即为3组，每组6个重复，每个重复20只乳鸽。试验期21 d。检测1、7、14、21日龄3个品种乳鸽的体重，十二指肠和空肠脂肪酶、蔗糖酶、胰蛋白酶、糜蛋白酶、麦芽糖酶活性以及回肠紧密连接蛋白ZO-1、Occludin的基因表达量。结果表明：1）不同品种间乳鸽体重差异不显著（P>0.05）。2）1日龄卡奴鸽的十二指肠糜蛋白酶、胰蛋白酶活性显著高于深王鸽（P<0.05），7日龄深王鸽、敖峰鸽的十二指肠脂肪酶活性显著高于卡奴鸽（P<0.05），14日龄敖峰鸽的十二指肠蔗糖酶活性以及深王鸽的十二指肠蔗糖酶、麦芽糖酶活性显著高于卡奴鸽（P<0.05）。3）1日龄卡奴鸽的空肠糜蛋白酶活性显著高于深王鸽（P<0.05），空肠蔗糖酶活性显著高于敖峰鸽（P<0.05），空肠麦芽糖酶、脂肪酶活性显著高于深王鸽和敖峰鸽（P<0.05）；14日龄深王鸽的空肠胰蛋白酶、蔗糖酶、麦芽糖酶活性显著高于卡奴鸽（P<0.05）；21日龄深王鸽的空肠糜蛋白酶、蔗糖酶、脂肪酶活性显著高于卡奴鸽（P<0.05）。4）不同品种间乳鸽的回肠ZO-1、Occludin的基因表达量差异不显著（P>0.05）。5）不同品种乳鸽的体重随日龄增加呈线性升高（P<0.05），十二指肠和空肠蔗糖酶、麦芽糖酶、脂肪酶活性随日龄增加呈线性增加（P<0.05），回肠ZO-1、Occlidin的基因表达量随日龄增加呈线性增加（P<0.05）。综上所述，深王鸽、敖峰鸽和卡奴鸽的体重均随日龄的增加线性增加，但相同日龄不同品种间无显著差异。不同品种的乳鸽在不同发育阶段具有不同的消化特性。不同品种乳鸽回肠紧密连接蛋白ZO-1、Occludin的基因表达量随日龄增加呈线性增加，但相同日龄不同品种间无显著差异。

关键词： 品种; 日龄; 乳鸽; 体重; 消化酶; 紧密连接蛋白

本文引用格式

马晓汶 , 田勇 , 李国勤 , 徐倩倩 , 陈晓燕 , 陶争荣 , 温积辉 , 崔世豪 , 卢立志 , 邹晓庭 . 不同品种乳鸽肠道消化酶活性及回肠闭合小环蛋白-1、闭锁蛋白基因表达的差异研究[J]. 动物营养学报, 2020 , 32(3) : 1334 -1343 . DOI: 10.3969/j.issn.1006-267x.2020.03.041

Abstract

The purpose of this study was to compare the differences of intestinal digestive enzyme activities and ileum zonula occludens-1 (ZO-1) and Occludin gene expression of different breeds of pigeon squabs. Each breeds of white king pigeons, carneau pigeons and Aofeng pigeons at 1 day of age were selected and contained 120 pigeon squabs, three breeds were three groups, each group contained 6 replicates and 20 pigeon squabs per replicate. The experiment lasted for 21 days. The body weight, activities of lipase, sucrase, trypsin, chymotrypsin, maltase in duodenum and jejunum, as well as the gene expressions of ileal tight junction protein ZO-1, Occludin of three breeds of pigeon squabs at 1, 7, 14 and 21 days of age were detected. The results showed as follows:1) there was no significant difference in body weight of pigeon squabs among breeds (P>0.05). 2) The activities of chymotrypsin and trypsin in duodenum of carneau pigeons at 1 day of age were significantly higher than those of white king pigeons (P<0.05), the duodenum lipase activity of white king pigeons and Aofeng pigeons at 7 days of age was significantly higher than that of carneau pigeons (P<0.05), and the duodenum sucrase activity of Aofeng pigeons and duodenum sucrose and maltase activities of white king pigeons at 14 days of age were significantly higher than those of carneau pigeons (P<0.05). 3) The jejunum chymotrypsin activity of carneau pigeons at 1 day of age was significantly higher than that of white king pigeons (P<0.05), the jejunum sucrase activity was significantly higher than that of Aofeng pigeons (P<0.05), and the activities of lipase and maltase in jejunum were significantly higher than those of white king pigeons and Aofeng pigeons (P<0.05). The activities of trypsin, sucrase and maltase in jejunum of white king pigeons at 14 days of age were significantly higher than those of carneau pigeons (P<0.05), and the activities of chymotrypsin, sucrase and lipase in jejunum of white king pigeons at 21 days of age were significantly higher than those of carneau pigeons (P<0.05). 4) There was no significant difference in gene expression levels of ZO-1 and Occludin in ileum of pigeon squabs between breeds (P>0.05). 5) The body weight of different breeds of pigeon squabs was linearly increased with the day of age increased (P<0.05), the activities of sucrase, maltase and lipase in duodenum and jejunum were linearly increased with the day of age increased (P<0.05), and the gene expression levels of ZO-1 and Occludin in ileum were linearly increased with the day of age increased (P<0.05). In conclusion, the body weight of white king pigeons, carneau pigeons and Aofeng pigeons are linearly increased with the day of age increased, but there was no significant difference among different breeds at the same day of age. Different breeds of pigeon squabs have different digestive characteristics at different developmental stages. The gene expression levels of ZO-1 and Occludin in ileum of different breeds of pigeon squabs are linearly increased with the day of age increased, but there was no significant difference among different breeds at the same day of age.

Key words： breeds; days of age; pigeon squabs; body weight; digestive enzyme; tight junction protein

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