动物营养学报 >

2020 , Vol. 32 >Issue 6: 2567 - 2574

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.016

猪营养与饲料 SWINE NUTRITION AND FEED

亚麻油对宁乡猪不同组织长链脂肪酸谱的影响

  • 邢月腾 ,
  • 郁元年 ,
  • 谢春艳 ,
  • 吴信 ,
  • 肖定福 ,
  • 杨志武 ,
  • 张彬
展开
  • 1. 湖南农业大学动物科学技术学院, 长沙 410128;
    2. 湖南农业大学资源环境学院, 长沙 410128;
    3. 中国科学院亚热带农业生态研究所, 长沙 410125;
    4. 宁乡市畜牧兽医水产局, 长沙 410600
邢月腾(1989-),男,河南郸城人,博士研究生,从事动物营养生理与代谢调控研究。E-mail:xingyuet@126.com

收稿日期: 2019-12-15

  网络出版日期: 2020-06-16

基金资助

国家自然科学基金项目（31872991，31902196）；湖南省重大专项（2015NK1002）；湖南省研究生科研创新项目（CX2016B277）；宁乡市人民政府与湖南农业大学战略性合作项目（宁乡猪基础研究）

收起

Effects of Linseed Oil on Long-Chain Fatty Acid Profile in Different Tissues of Ningxiang Pigs

  • XING Yueteng ,
  • YU Yuannian ,
  • XIE Chunyan ,
  • WU Xin ,
  • XIAO Dingfu ,
  • YANG Zhiwu ,
  • ZHANG Bin
Expand
  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China;
    3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    4. Ningxiang Animal Husbandry and Veterinary Bureau, Changsha 410600, China

Received date: 2019-12-15

  Online published: 2020-06-16

Fold

摘要

本试验旨在研究亚麻油对宁乡猪背肌和3种不同脂肪组织中长链脂肪酸谱的影响，并探究脂肪酸生成的潜在机制。选取日龄相近、平均体重（43.21±0.64） kg的宁乡阉母猪30头，随机分为2组，每组5个重复，每个重复3头。对照组饲喂基础饲粮，试验组在基础饲粮中添加2%亚麻油，试验期56 d。结果显示：与对照组相比，饲粮中添加2%亚麻油后，宁乡猪背肌中花生一烯酸（C20：1）、γ-亚麻酸（C18：3n-6）、α-亚麻酸（C18：3n-3）含量显著提高（P<0.05），花生四烯酸（C20：4n-6）含量显著降低（P<0.05）；饲粮中添加2%亚麻油对3个脂肪组织中脂肪酸含量的影响与背肌趋于一致，如能显著提高背脂、腹脂和肾周脂中亚油酸（C18：2n-6c）、C18：3n-6、C18：3n-3、二十碳三烯酸（C20：3n-6）含量（P<0.05），降低背脂和腹脂中油酸（C18：1n-9c）（P<0.05）、C20：4n-6（0.05≤P<0.10）及肾周脂中硬脂酸（C18：0）含量（P<0.05）；同时，饲粮中添加2%亚麻油还能显著降低3种脂肪组织中n-6/n-3多不饱和脂肪酸（PUFA）比值（P<0.05），使3种脂肪组织中平均n-6/n-3 PUFA比值降到5.92。此外，饲粮中添加2%亚麻油能显著下调背脂中乙酰辅酶A羧化酶α（ACCα）、γ-过氧化酶活化增生受体（PPARγ）和脂肪酸去饱和酶2（FADS2）基因的表达（P<0.05），对α-过氧化酶活化增生受体（PPARα）基因的表达也有抑制趋势（0.05≤P<0.10）。上述结果表明，饲粮中添加2%亚麻油能显著提高宁乡猪背肌、背脂、腹脂和肾周脂中C18：3n-3含量，使3种脂肪组织中平均n-6/n-3 PUFA比值降至《中国居民膳食指南（2016）》推荐范围（4~6）内；这可能是通过调控细胞分化及抑制脂肪酸从头合成途径实现的。

关键词： 亚麻油; 长链脂肪酸; 脂代谢; 宁乡猪

本文引用格式

邢月腾 , 郁元年 , 谢春艳 , 吴信 , 肖定福 , 杨志武 , 张彬 . 亚麻油对宁乡猪不同组织长链脂肪酸谱的影响[J]. 动物营养学报, 2020 , 32(6) : 2567 -2574 . DOI: 10.3969/j.issn.1006-267x.2020.06.016

Abstract

This experiment was conducted to study the linseed oil (LO) on fatty acid profile in longissimus dorsi muscle (LDM) and three different adipose tissues of Ningxiang pigs, and then investigate the underlying lipogenesis mechanisms. Thirty castrated female pigs with a similar age and average body weight of (43.21±0.64) kg were randomly allocated into 2 groups with 5 repetitions per group and 3 pigs per repetition. Pigs in control group were fed a basal diet, and those in experimental group were fed the basal diet supplemented with 2% LO for 56 days. The results showed that dietary supplemented with 2% LO significantly increased the contents of eicosenoic acid (C20:1), γ-linolenic acid (C18:3n-6) and α-linolenic acid (C18:3n-3) in LDM (P<0.05), and significantly decreased the content of arachidonic acid (C20:4n-6) in LDM compared with control group (P<0.05). The fatty acid contents in 3 adipose tissues influenced by 2% LO were analogous to those found in the LDM. The contents of linoleic acid (C18:2n-6c), C18:3n-6, C18:3n-3 and epoxyeicosatrienoic acid (C20:3n-6) in dorsal subcutaneous adipose (DSA), abdominal subcutaneous adipose (ASA) and perirenal adipose (PA) were significantly increased (P<0.05) accompanied by a corresponding decrease in oleic acid (C18:1n-9c) (P<0.05), C20:4n-6 (0.05≤P<0.10) in DSA and APA, as well as stearic acid (C18:0) in PA (P<0.05). Meanwhile, dietary supplemented with 2% LO could significantly the ratio of n-6/n-3 polyunsaturated fatty acids (PUFA) in 3 adipose tissues (P<0.05), and make the average n-6/n-3 PUFA ratio reduce to 5.92. Furthermore, dietary supplemented with 2% LO could down-regulate the expression of acetyl coenzyme A carboxylase α (ACCα), peroxisome proliferators-activated receptor γ (PPARγ), fatty acid desaturase 2 (FADS2) genes in DSA (P<0.05), and had an inhibitory tendency on the expression of peroxisome proliferators-activated receptor α (PPARα) gene in DSA (0.05≤P<0.10). These results indicate that dietary supplemented with 2% LO can significantly increase the content of C18:3n-3 in LDM, DSA, ASA and PA of Ningxiang pigs, and make the average n-6/n-3 PUFA ratio in three adipose tissues down to 5.92, which in the recommended range (4 to 6) of Dietary Guidelines for Chinese Residents (2016). The regulation of LO on lipid metabolism may be achieved through regulating the de novo synthesis of fatty acids and cell differentiation.

Key words： linseed oil; long-chain fatty acid; lipid metabolism; Ningxiang pigs

参考文献

[1] 周晶晶,李家速,王奇金.Omega-3多不饱和脂肪酸对脂肪代谢调节作用研究进展[J].第二军医大学学报,2019,40(1):68-73.
[2] SAINI R K,KEUM Y S.Omega-3 and omega-6 polyunsaturated fatty acids:dietary sources,metabolism,and significance-a review[J].Life Sciences,2018,203:255-267.
[3] HE Q,REN P,KONG X,et al.Comparison of serum metabolite compositions between obese and lean growing pigs using an NMR-based metabonomic approach[J].The Journal of Nutritional Biochemistry,2012,23(2):133-139.  
[4] JIANG Q,LI C,YU Y,et al.Comparison of fatty acid profile of three adipose tissues in Ningxiang pigs[J].Animal Nutrition,2018,4(3):256-259.  
[5] RAJ S,SKIBA G,WEREMKO D,et al.The relationship between the chemical composition of the carcass and the fatty acid composition of intramuscular fat and backfat of several pig breeds slaughtered at different weights[J].Meat Science,2010,86(2):324-330.  
[6] SOBOL M,SKIBA G,RAJ S.Effect of n-3 polyunsaturated fatty acid intake on its deposition in the body of growing-finishing pigs[J].Animal Feed Science and Technology,2015,208:107-118.
[7] 蔡传江,车向荣,赵克斌,等.亚麻油对育肥猪肉品质及脂肪组织脂肪酸组成的影响[J].畜牧兽医学报,2009,40(12):1755-1760.
[8] CORINO C,ROSSI R,CANNATA S,et al.Effect of dietary linseed on the nutritional value and quality of pork and pork products:systematic review and Meta-analysis[J].Meat Science,2014,98(4):679-688.  
[9] 邓波,门小明,吴杰,等.亚麻籽对生长育肥猪生长性能、胴体性状、肉质和脂肪酸组成的影响[J].动物营养学报,2019,31(9):4024-4032.
[10] XING Y,WU X,XIE C,et al.Meat quality and fatty acid profiles of Chinese Ningxiang pigs following supplementation with N-carbamylglutamate[J].Animals,2020,10(1):88.
[11] 喻文娟,侯静文,朱邦尚.外标-气相色谱-质谱法准确测定猪肉中的14种脂肪酸[J].分析仪器,2012(3):10-16.
[12] APPLE J K,MAXWELL C V,GALLOWAY D L,et al.Interactive effects of dietary fat source and slaughter weight in growing-finishing swine:Ⅲ.Carcass and fatty acid compositions[J].Journal of Animal Science,2009,87(4):1441-1454.  
[13] WOOD J D,ENSER M,FISHER A V,et al.Fat deposition,fatty acid composition and meat quality:a review[J].Meat Science,2008,78(4):343-358.  
[14] 中国营养学会.中国居民膳食指南2016[M].北京:人民卫生出版社,2016.
[15] HUANG F R,ZHAN Z P,LUO J,et al.Duration of dietary linseed feeding affects the intramuscular fat,muscle mass and fatty acid composition in pig muscle[J].Livestock Science,2008,118(1/2):132-139.
[16] DANNENBERGER D,NUERNBERG G,NUERNBERG K,et al.Effects of diets supplemented with n-3 or n-6 PUFA on pig muscle lipid metabolites measured by non-targeted LC-MS lipidomic profiling[J].Journal of Food Composition and Analysis,2017,56:47-54.
[17] NUERNBERG K,FISCHER K,NUERNBERG G,et al.Effects of dietary olive and linseed oil on lipid composition, meat quality, sensory characteristics and muscle structure in pigs[J].Meat Science,2005,70(1):63-74.  
[18] GUILLEVIC M,KOUBA M,MOUROT J.Effect of a linseed diet or a sunflower diet on performances,fatty acid composition,lipogenic enzyme activities and stearoyl-CoA-desaturase activity in the pig[J].Livestock Science,2009,124(1/2/3):289-294.
[19] KOUBA M,ENSER M,WHITTINGTON F M,et al.Effect of a high-linolenic acid diet on lipogenic enzyme activities,fatty acid composition,and meat quality in the growing pig[J].Journal of Animal Science,2003,81(8):1967-1979.  
[20] MOUROT J,KOUBA M,PEINIAU P.Comparative study of in vitro lipogenesis in various adipose tissues in the growing domestic pig (Sus domesticus)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1995,111(3):379-384.  
[21] KIM J S,INGALE S L,LEE S H,et al.Impact of dietary fat sources and feeding level on adipose tissue fatty acids composition and lipid metabolism related gene expression in finisher pigs[J].Animal Feed Science and Technology,2014,196:60-67.
[22] REY A I,LOPEZ-BOTE C J,KERRY J P,et al.Modification of lipid composition and oxidation in porcine muscle and muscle microsomes as affected by dietary supplementation of n-3 with either n-9 or n-6 fatty acids and α-tocopheryl acetate[J].Animal Feed Science and Technology,2004,113(1/2/3/4):223-238.
[23] REALINI C E,DURAN-MONTGÉ P,LIZARDO R,et al.Effect of source of dietary fat on pig performance,carcass characteristics and carcass fat content,distribution and fatty acid composition[J].Meat Science,2010,85(4):606-612.  
[24] DE TONNAC A,KARIM-LUISSET S,MOUROT J.Effect of different dietary linseed sources on fatty acid composition in pig tissues[J].Livestock Science,2017,203:124-131.
[25] MONZIOLS M,BONNEAU M,DAVENEL A,et al.Comparison of the lipid content and fatty acid composition of intermuscular and subcutaneous adipose tissues in pig carcasses[J].Meat Science,2007,76(1):54-60.  
[26] DE TONNAC A,LABUSSIÈRE E,VINCENT A,et al.Effect of α-linolenic acid and DHA intake on lipogenesis and gene expression involved in fatty acid metabolism in growing-finishing pigs[J].British Journal of Nutrition,2016,116(1):7-18.  
[27] HILLER B,HERDMANN A,NUERNBERG K.Dietary n-3 fatty acids significantly suppress lipogenesis in bovine muscle and adipose tissue:a functional genomics approach[J].Lipids,2011,46(7):557-567.  
[28] LIU C Y,GRANT A L,KIM K H,et al.Porcine somatotropin decreases acetyl-CoA carboxylase gene expression in porcine adipose tissue[J].Domestic Animal Endocrinology,1994,11(1):125-132.  
[29] SMITH S,WITKOWSKI A,JOSHI A K.Structural and functional organization of the animal fatty acid synthase[J].Progress in Lipid Research,2003,42(4):289-317.  
[30] SPURLOCK M E,HOUSEKNECHT K L,PORTOCARRERO C P,et al.Regulation of PPARγ but not obese gene expression by dietary fat supplementation[J].Journal of Nutritional Biochemistry,2000,11(5):260-266.  
[31] DING S T,SCHINCKEL A P,WEBER T E,et al.Expression of porcine transcription factors and genes related to fatty acid metabolism in different tissues and genetic populations[J].Journal of Animal Science,2000,78(8):2127-2134.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2