动物营养学报 >

2020 , Vol. 32 >Issue 6: 2611 - 2623

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.020

禽营养与饲料 POULTRY NUTRITION AND FEED

饲粮添加L-谷氨酰胺对脂多糖刺激肉鸡血浆生化指标、免疫性能、肠道炎症因子表达及黏膜免疫的影响

  • 张柏林 ,
  • 杨乾 ,
  • 刘宁 ,
  • 宋培勇 ,
  • 谢宇潇 ,
  • 王庆容 ,
  • 孙泽威
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  • 1. 遵义师范学院生物与农业科技学院, 赤水河流域特色动物资源保护与利用重点实验室, 遵义 523006;
    2. 吉林农业大学动物科技学院, 动物生产及产品质量安全教育部重点实验室, 长春 130118
张柏林(1981-),男,山东烟台人,副教授,博士,研究方向为动物营养与生理调控。E-mail:zhangbolin@zync.edu.cn

收稿日期: 2020-01-31

  网络出版日期: 2020-06-16

基金资助

贵州省教育厅项目（黔教合KY字[2016]258）；贵州省科技厅项目（黔科合基础2017[1205]）；贵州省高层次创新型人才（遵市科合人才[2017]10号）；遵义市校联合基金（遵市科合计[2018]08号）；遵义市"15851"人才项目（2050020213#）；贵州省服务农村产业革命项目（遵师合农村产业字201905）

收起

Effects of Dietary L-Glutamine Supplementation on Plasma Biochemical Parameters, Immune Performance, Intestinal Inflammatory Factors Expression and Mucosal Immune of Broilers Challenged by Lipopolysaccharide

  • ZHANG Bolin ,
  • YANG Qian ,
  • LIU Ning ,
  • SONG Peiyong ,
  • XIE Yuxiao ,
  • WANG Qingrong ,
  • SUN Zewei
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  • 1. Key Laboratory of Conservation and Utilization of Characteristic Animal Resources in Chishui River Basin, Department of Biology and Agriculture, Zunyi Normal College, Zunyi 523006, China;
    2. Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China

Received date: 2020-01-31

  Online published: 2020-06-16

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摘要

本试验旨在研究饲粮添加L-谷氨酰胺（L-Gln）对脂多糖（LPS）刺激肉鸡血浆生化指标、免疫性能、肠道炎症因子表达及黏膜免疫的影响。选择健康状况良好、体重相近的1日龄爱拔益加（AA）肉鸡120只，按2×2试验设计，随机分为4个组（A、B、C及D组），每组5个重复，每个重复6只鸡。A、C组饲喂对照饲粮（基础饲粮中添加1.22%L-丙氨酸），B、D组饲喂L-Gln饲粮（基础饲粮中添加1%L-Gln）。第16天及第21天，B、D组肉鸡分别腹腔注射LPS （500 μg/kg BW），A、C组肉鸡腹腔注射等量的生理盐水，注射前12 h停料不停水。试验期21 d。结果表明：1） LPS刺激显著增加了肉鸡血浆中总蛋白（TP）、尿酸（UA）含量及谷丙转氨酶（ALT）、谷草转氨酶（AST）活性（P<0.05），显著降低了胸腺指数、脾脏指数及法氏囊指数（P<0.05），显著降低了十二指肠相对长度、十二指肠相对重量，空肠相对长度、空肠相对重量、回肠相对长度及回肠相对重量（P<0.05），显著增加了十二指肠、空肠及回肠肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）及白细胞介素-6（IL-6） mRNA表达水平（P<0.05），显著增加了空肠黏膜分泌型免疫球蛋白A含量（P<0.05），显著增加了空肠黏膜黏蛋白1（MUC1）及黏蛋白2（MUC2） mRNA表达水平（P<0.05）。2）饲粮添加L-Gln显著降低了肉鸡TP、UA含量及ALT、AST活性（P<0.05），显著增加了胸腺指数、脾脏指数及法氏囊指数（P<0.05），显著增加了十二指肠相对长度、十二指肠相对重量，空肠相对长度、空肠相对重量、回肠相对长度及回肠相对重量（P<0.05），显著降低了十二指肠TNF-α及IL-6 mRNA表达水平（P<0.05），显著降低了空肠及回肠TNF-α、IL-1β mRNA表达水平（P<0.05），显著增加了空肠黏膜sIgA含量（P<0.05），显著降低了空肠黏膜MUC1及MUC2 mRNA表达水平（P<0.05）。综上所述，饲粮中添加1%L-Gln能够提高肉鸡免疫性能，增加肠道黏膜免疫机能，从而在一定程度上有效缓解LPS刺激引起的肉鸡肠道炎症反应。

关键词： L-谷氨酰胺; 脂多糖刺激; 免疫性能; 炎症反应; 肠道黏膜免疫

本文引用格式

张柏林 , 杨乾 , 刘宁 , 宋培勇 , 谢宇潇 , 王庆容 , 孙泽威 . 饲粮添加L-谷氨酰胺对脂多糖刺激肉鸡血浆生化指标、免疫性能、肠道炎症因子表达及黏膜免疫的影响[J]. 动物营养学报, 2020 , 32(6) : 2611 -2623 . DOI: 10.3969/j.issn.1006-267x.2020.06.020

Abstract

The present study aimed to investigate the effects of dietary L-glutamine (L-Gln)supplementation on plasma biochemical parameters, immune performance, intestinal inflammatory factors expression and mucosal immune of broilers by lipopolysaccharide (LPS) challenge. A total of 120 one-day-old Arbor Acres (AA) broilers with good health and similar initial body weight were arranged in a 2×2 factorial design to 4 groups (groups A, B, C and D) with 5 replicates per group and 6 broilers per replicate. Broilers in groups A and C were fed the control diets (basal diet supplemented with 1.22% alanine), and broilers in groups B and D were fed the L-Gln diets (basal diet supplemented with 1% L-Gln). At day 16 and day 21, broilers in groups B and D were administered with LPS (500 μg/kg BW) by intraperitoneal injection, and broilers in groups B and D were administered with equivalent saline by intraperitoneal injection. The diet rather than water was cut off before 12 h of injection. The experiment lasted for 21 days. The results were as follows: 1) LPS challenge significantly increased the total protein (TP), uric acid (UA) contents and alanine aminotransferase (ALT), aspartate transaminase (AST) activities in plasma of broilers (P<0.05), significantly decreased the thymus index, spleen index and bursa of Fabricius index (P<0.05), significantly decreased the relative length of duodenum, relative weight of duodenum, relative length of jejunum, relative weight of jejunum, relative length of ileum and relative weight of ileum (P<0.05), significantly increased the mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in duodenum, jejunum and ileum (P<0.05), significantly increased the content of secretory immunoglobulin A (sIgA) in jejunal mucosa (P<0.05), and significantly increased the mRNA expression levels of mucin 1 (MUC1) and mucin 2 (MUC2) in jejunal mucosa (P<0.05). 2) Dietary L-Gln supplementation significantly decreased the TP, UA contents and ALT, AST activities in plasma of broilers (P<0.05), significantly increased the thymus index, spleen index and bursa of Fabricius index (P<0.05), significantly increased the relative length of duodenum, relative weight of duodenum, relative length of jejunum, relative weight of jejunum, relative length of ileum and relative weight of ileum (P<0.05), significantly decreased the mRNA expression levels of TNF-α and IL-6 in duodenum (P<0.05), significantly decreased the mRNA expression levels of TNF-α and IL-1β in jejunum and ileum (P<0.05), significantly decreased the content of sIgA in jejunal mucosa (P<0.05), and significantly decreased the mRNA expression levels of MUC1 and MUC2 in jejunal mucosa (P<0.05). In conclusion, dietary 1% L-Gln supplementation can improve the immune performance of broilers, enhance the intestinal mucosal immune function, and can partly effectively alleviate intestinal inflammatory responses of broilers induced by LPS.

Key words： L-glutamine; LPS challenge; immune performance; inflammatory response; intestinal mucosal immune

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