动物营养学报 >

2020 , Vol. 32 >Issue 6: 2638 - 2649

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.023

禽营养与饲料 POULTRY NUTRITION AND FEED

饲粮添加25-羟基维生素D3、植物精油和苯甲酸对蛋鸡生产性能、蛋品质和肠道形态的影响

  • 弓浩杰 ,
  • 丁雪梅 ,
  • 白世平 ,
  • 曾秋凤 ,
  • 张克英 ,
  • 申攀 ,
  • 王建萍
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  • 四川农业大学动物营养研究所, 教育部动物抗病营养重点实验室, 动物抗病营养与饲料农业农村部重点实验室, 动物抗病营养四川省重点实验室, 成都 611130
弓浩杰(1997-),男,山西太原人,硕士研究生,动物营养与饲料科学专业。E-mail:ghjie2904@163.com

收稿日期: 2019-12-13

  网络出版日期: 2020-06-16

基金资助

国家十三五重点研发计划（2017YFD0500500）；国家自然科学基金项目（31872792）；四川省科技厅国际合作项目（2019YFH0062）；四川省蛋鸡产业链项目（2018NZ0009，2014NZ0002，2014NZ0043，2013NZ0054）

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Effects of 25-Hydroxyvitamin D3, Plant Essential Oil and Benzoic Acid on Performance, Egg Quality and Intestinal Morphology of Laying Hens

  • GONG Haojie ,
  • DING Xuemei ,
  • BAI Shiping ,
  • ZENG Qiufeng ,
  • ZHANG Keying ,
  • SHEN Pan ,
  • WANG Jianping
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  • Sichuan Key Laboratory of Animal Disease Resistance Nutrition, Key Laboratory of Animal Disease-Resistance Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2019-12-13

  Online published: 2020-06-16

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摘要

本试验旨在研究饲粮添加25-羟基维生素D3（HyD）、植物精油和苯甲酸对蛋鸡生产性能、蛋品质和肠道形态的影响。试验采用单因子试验设计，选取45周龄1 200只产蛋后期罗曼粉壳蛋鸡，随机分为5个组，每组10个重复，每个重复24只鸡。对照组饲喂玉米-豆粕型基础饲粮，试验组分别饲喂在基础饲粮中添加0.069 mg/kg HyD （HYD组）、0.069 mg/kg HyD+150 mg/kg植物精油（PEO组）、1 000 mg/kg苯甲酸（BA1组）和2 000 mg/kg苯甲酸（BA2组）的饲粮。试验预试期4周，正试期16周。结果表明：1）与对照组相比，PEO组、BA1组和BA2组蛋鸡第1~4周平均日采食量显著降低（P<0.05），同时料蛋比也显著降低（P<0.05）；BA2组蛋鸡第1~4周和第13~16周平均蛋重显著降低（P<0.05）。2）与对照组相比，PEO组第4周蛋壳厚度显著提高（P<0.05），试验组第8周蛋壳厚度均显著提高（P<0.05），PEO组、BA1组和BA2组第16周蛋白高度和哈氏单位显著提高（P<0.05）。3）与对照组相比，试验组鸡蛋破壳蛋率和暗斑蛋发生率降低。4）与对照组相比，HYD组、PEO组和BA1组蛋鸡十二指肠绒毛高度显著增加（P<0.05），PEO组蛋鸡十二指肠绒毛高度/隐窝深度值显著增加（P<0.05）；PEO组蛋鸡空肠绒毛高度显著高于对照组（P<0.05）。5）与对照组相比，HYD组和PEO组蛋鸡胫骨强度显著提高（P<0.05）。6）与对照组相比，HYD组和PEO组蛋鸡血清促卵泡素和钙含量显著增加（P<0.05）。结果提示，蛋鸡饲粮中添加HyD或同时添加HyD和植物精油可以提高产蛋后期生产性能、蛋品质和胫骨质量并能改善肠道形态，而HyD、HyD+植物精油和低剂量的苯甲酸可以降低破壳蛋率和暗斑蛋的发生率，但短期（前4周）高剂量的苯甲酸会降低蛋鸡的采食量，从而影响蛋鸡产蛋性能。

关键词： 蛋鸡; 生产性能; 蛋品质; 肠道形态; 胫骨质量; 25-羟基维生素D3; 苯甲酸

本文引用格式

弓浩杰 , 丁雪梅 , 白世平 , 曾秋凤 , 张克英 , 申攀 , 王建萍 . 饲粮添加25-羟基维生素D3、植物精油和苯甲酸对蛋鸡生产性能、蛋品质和肠道形态的影响[J]. 动物营养学报, 2020 , 32(6) : 2638 -2649 . DOI: 10.3969/j.issn.1006-267x.2020.06.023

Abstract

The experiment was conducted to study the effects of 25-hydroxyvitamin D3 (HyD), plant essential oil and benzoic acid on performance, egg quality and intestinal morphology of laying hens. Using a single-factor design, a total of 1 200 Lohmann Pink-eggshell layers during late-laying phase (45-week-old) were randomly allotted into 5 groups with 10 replicates for each group and 24 chickens for each replicate. Chicks in the control group were fed a corn-soybean meal basal diet, while those in the experimental groups were fed the basal diet supplemented with 0.069 mg/kg HyD (HYD group), 0.069 mg/kg HyD+150 mg/kg plant essential oil (PEO group), 1 000 mg/kg benzoic acid (BA1 group) and 2 000 mg/kg benzoic acid (BA2 group), respectively. The pre-trial period was 4 weeks and the trial period was 16 weeks. The results showed as follows: 1) compared with the control group, the average daily feed intake of laying hens from 1 to 4 weeks in PEO group, BA1 group and BA2 group was significantly decreased (P<0.05), and the feed-to-egg ratio was also significantly decreased (P<0.05). The average egg weight of laying hens from 1 to 4 weeks and 13 to 16 weeks in BA2 group was significantly decreased (P<0.05). 2) Compared with the control group, the egg shell thickness in PEO group was significantly increased at week 4 (P<0.05), the egg shell thickness in the experimental groups was significantly increased at week 8 (P<0.05), and the albumen height and Haugh unit in PEO group, BA1 group and BA2 group were significantly increased at week 16 (P<0.05). 3) Compared with the control group, the breaking egg rate and the incidence of translucent eggs in the experimental groups were decreased. 4) Compared with the control group, the villus height in duodenum of layers in HYD group, PEO group and BA1 group was significantly increased (P<0.05), the villus height to crypt depth ratio in duodenum of layers in PEO group was significantly increased (P<0.05), and the villus height in jejunum of layers in PEO group was significantly increased (P<0.05). 5) Compared with the control group, the tibia strength of layers in HYD group and PEO group was significantly improved (P<0.05). 6) Compared with the control group, the serum contents of follicle-stimulating hormone and calcium of layers in HYD group and PEO group were significantly increased (P<0.05). The results indicate that dietary HyD or dietary HyD and plant essential oil can improve the performance, egg and tibia quality and intestinal morphology of laying hens during late laying period, while dietary HyD, HyD+plant essential oil and low dose of benzoic acid can reduce the breaking egg rate and the incidence of translucent eggs, but the high dose of benzoic acid can reduce the feed intake of laying hens in the first 4 weeks, thus affecting the egg production performance.

Key words： laying hens; performance; egg quality; intestinal morphology; tibia quality; 25-hydroxyvitamin D3; benzoic acid

参考文献

[1] 杨建国.养殖密度对蛋鸡生产性能及影响[J].现代农业研究,2017(7):29.
[2] RATH N C,HUFF G R,HUFF W E,et al.Factors regulating bone maturity and strength in poultry[J].Poultry Science,2000,79(7):1024-1032.  
[3] LIN H,JIAO H C,BUYSE J,et al.Strategies for preventing heat stress in poultry[J].World’s Poultry Science Journal,2006,62(1):71-86.
[4] HOLICK M F,CHEN T C.Vitamin D deficiency:a worldwide problem with health consequences[J].The American Journal of Clinical Nutrition,2008,87(4):1080S-1086S.
[5] 朱建军,袁建敏,田科雄,等.25-羟胆钙化醇与维生素D3对北京鸭生产性能和骨骼发育的影响[J].中国畜牧杂志,2011,47(3):30-35.
[6] 韩进诚,姚军虎,郑永祥.25-羟基维生素D3在动物营养中的应用[J].饲料工业,2013,34(10):56-59.
[7] 翁江来,马长伟.苯甲酸和苯甲酸钠在肉制品中应用的探讨[J].肉类研究,2005(5):48-50.
[8] EVANS W C,FUCHS G.Anaerobic degradation of aromatic compounds[J].Annual Review of Microbiology,1988,42:289-317.
[9] MROZ Z.Organic acids as potential Alternatives to antibiotic growth promoters for pigs[J].Advances in Pork Production,2005,16:169-182.
[10] 张波,孙得发,袁磊,等.包被苯甲酸对肉鸡生长性能及器官发育的影响[J].中国家禽,2017,39(8):增页2-增页5.
[11] GIANNENAS I A,PAPANEOPHYTOU C P,TSALIE E,et al.The effects of benzoic acid and essential oil compounds in combination with protease on the performance of chickens[J].Journal of Animal and Feed Sciences,2014,23(1):73-81.  
[12] ABDEL-WARETH A A A,LOHAKARE J D.Effect of dietary supplementation of peppermint on performance,egg quality,and serum metabolic profile of Hy-Line Brown hens during the late laying period[J].Animal Feed Science and Technology,2014,197:114-120.
[13] ABDEL-WARETH A A A.Effect of dietary supplementation of thymol,synbiotic and their combination on performance,egg quality and serum metabolic profile of Hy-Line Brown hens[J].British Poultry Science,2016,57(1):114-122.  
[14] ALÇIÇEK A,BOZKURT M,CABUK M.The effect of a mixture of herbal essential oils,an organic acid or a probiotic on broiler performance[J].South African Journal of Animal Science,2004,34(4):217-222.
[15] LEE K W,EVERTS H,KAPPERT H J,et al.Growth performance of broiler chickens fed a carboxymethyl cellulose containing diet with supplemental carvacrol and/or cinnamaldehyde[J].International Journal of Poultry Science,2004,3(9):619-622.  
[16] 袁萍,王国亮,龚复俊,等.3种植物精油对霉菌的抑制作用[J].武汉植物学研究,2001,19(6):521-523.
[17] BOTSOGLOU N A,FLOROU-PANERI P,CHRISTAKI E,et al.Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast,thigh and abdominal fat tissues[J].British Poultry Science,2002,43(2):223-230.  
[18] KESHAVARZ K.The effect of different levels of vitamin C and cholecalciferol with adequate or marginal levels of dietary calcium on performance and eggshell quality of laying hens[J].Poultry Science,1996,75(10):1227-1235.  
[19] KESHAVARZ K.A comparison between cholecalciferol and 25-OH-cholecalciferol on performance and eggshell quality of hens fed different levels of calcium and phosphorus[J].Poultry Science,2003,82(9):1415-1422.  
[20] 贾洪阁,魏时来,杨德志,等.日粮中25-OH-D3、VD3不同水平组合对肉仔鸡生长性能的影响[J].中国畜牧兽医,2007,34(8):10-13.
[21] 梁彦明,王浩.饮水添加25-OH-D3对肉仔鸡生产性能的影响[J].饲料与畜牧,2009(1):48-50.
[22] 王光富,梁赛赛,李雪媛,等.植物精油和有机酸对21至35周龄蛋鸡生产性能及蛋品质的影响[J].家畜生态学报,2018,39(4):22-26.
[23] BÖLÜKBA?I? C,ERHAN M K.Effect of dietary thyme (Thymus vulgaris) on laying hens performance and Escherichia coli (E.coli) concentration in feces[J].International Journal of Natural & Engineering Sciences,2007,1(2):55-58.
[24] JÓZEFIAK D,KACZMAREK S,BOCHENEK M,et al.A note on effect of benzoic acid supplementation on the performance and microbiota population of broiler chickens[J].Journal of Animal and Feed Sciences,2007,16(2):252-256.  
[25] JÓZEFIAK D,KACZMAREK S,RUTKOWSKI A.The effects of benzoic acid supplementation on the performance of broiler chickens[J].Journal of Animal Physiology and Animal Nutrition,2010,94(1):29-34.  
[26] AMAECHI N,ANUEYIAGU C F.The effect of dietary benzoic acid supplementation on growth performance and intestinal wall morphology of broilers[J].Online Journal of Animal and Feed Research,2012,1(5):401-404.
[27] 陈杰,沈一茹,张珊,等.酸化剂对蛋鸡产蛋性能、蛋品质和经济效益的影响[J].中国家禽,2015,37(8):58-60.
[28] KIM E J,AHN B K,KANG C W.Dietary effects of varying levels of calcium and 25-hydroxycholecalciferol on hatching egg production and eggshell quality in aged egg-type breeder hens[J].Journal of Animal Science and Technology,2009,51(4):295-306.  
[29] MCLOUGHLIN C P,SOARES JR J H.A study of the effects of 25 hydroxycholecalciferol and calcium source on egg shell quality[J].Poultry Science,1976,55(4):1400-1410.  
[30] 赵守亮.破骨细胞形成和骨吸收机理[J].牙体牙髓牙周病学杂志,1996,6(1):53-55.
[31] ?WIATKIEWICZ S,KORELESKI J,KOPOWSKI J.Effect of phytase and 25-hydroxycholecalciferol on performance and bone quality in broiler chickens[J].Medycyna Weterynaryjna,2006,62(1):81-84.
[32] 韩进诚,王建国,陈冠华,等.肉鸡日粮中1α-羟基维生素D3与25-羟基维生素D3生物学效价比较研究[J].中国畜牧兽医,2015,42(12):3246-3252.
[33] MORRIS A,SHANMUGASUNDARAM R,MCDONALD J,et al.Effect of in vitro and in vivo 25-hydroxyvitamin D treatment on macrophages,T cells,and layer chickens during a coccidia challenge[J].Journal of Animal Science,2015,93(6):2894-2903.  
[34] SAMIULLAH S,ROBERTS J R,CHOUSALKAR K.Eggshell color in brown-egg laying hens—a review[J].Poultry Science,2015,94(10):2566-2575.  
[35] BOZKURT M,KÜÇÜKYILMAZ K,PAMUKÇU M,et al.Long-term effects of dietary supplementation with an essential oil mixture on the growth and laying performance of two layer strains[J].Italian Journal of Animal Science,2012,11(1):e5.
[36] 张安,王来娣,彭艳,等.植物精油混合物对海兰褐蛋鸡生产性能、蛋品质、卵泡发育及血清生殖激素指标的影响[J].动物营养学报,2018,30(4):1489-1494.
[37] 王文杰,穆淑琴,张敬,等.木醋酸对鸡产蛋性能及蛋品质影响研究[J].中国饲料,2004(9):32-33.
[38] 赵旭,迟强伟,沈一茹,等.饮水型酸化剂对蛋鸡生产性能、蛋品质及血清生化指标的影响[J].中国家禽,2018(12):30-33.
[39] ZHANG A W,LEE B D,LEE S K,et al.Effects of yeast (Saccharomyces cerevisiae) cell components on growth performance,meat quality,and ileal mucosa development of broiler chicks[J].Poultry Science,2005,84(7):1015-1021.  
[40] CHOU S H,CHUNG T K,YU B.Effects of supplemental 25-hydroxycholecalciferol on growth performance,small intestinal morphology,and immune response of broiler chickens[J].Poultry Science,2009,88(11):2333-2341.  
[41] JANG I S,KO Y H,KANG S Y,et al.Effect of a commercial essential oil on growth performance,digestive enzyme activity and intestinal microflora population in broiler chickens[J].Animal Feed Science and Technology,2007,134(3/4):304-315.
[42] YANG Y F,ZHAO L L,SHAO Y X,et al.Effects of dietary graded levels of cinnamon essential oil and its combination with bamboo leaf flavonoid on immune function,antioxidative ability and intestinal microbiota of broilers[J].Journal of Integrative Agriculture,2019,18(9):2123-2132.  
[43] 宋凡春.包被苯甲酸和木聚糖酶对肉鸡生产性能和肠道健康的影响[D].硕士学位论文.泰安:山东农业大学,2016.
[44] GIANNENAS I,PAPANEOPHYTOU C P,TSALIE E,et al.Dietary supplementation of benzoic acid and essential oil compounds affects buffering capacity of the feeds,performance of turkey poults and their antioxidant status,pH in the digestive tract,intestinal microbiota and morphology[J].Asian-Australasian Journal of Animal Sciences,2014,27(2):225-236.  
[45] 黄灵杰,张克英,白世平,等.苯甲酸对1-21日龄肉鸡生长性能和肠道健康的影响[J].动物营养学报,2019,31(6):2816-2822.
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