水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

饥饿胁迫对彭泽鲫幼鱼形体指标、肌肉脂肪酸组成和肝脏脂蛋白脂酶基因表达的影响

  • 陈文静 ,
  • 丁立云 ,
  • 邓勇辉 ,
  • 肖俊 ,
  • 章海鑫 ,
  • 王生 ,
  • 巫伟华
展开
  • 江西省水产科学研究所, 南昌 330039
陈文静(1965-),女,江西九江人,研究员,硕士,从事水产养殖和营养研究。E-mail:418215117@qq.com

收稿日期: 2019-11-19

  网络出版日期: 2020-06-16

基金资助

江西省重点研发计划(20181BBF60016,20161ACF60020);江西省现代农业产业技术体系(JXARS-03)

Effects of Starvation Stress on Physical Indices, Muscle Fatty Acid Composition and Liver Lipoprotein Lipase Gene Expression of Juvenile Crucian Carp (Carassius auratus var. Pengze)

  • CHEN Wenjing ,
  • DING Liyun ,
  • DENG Yonghui ,
  • XIAO Jun ,
  • ZHANG Haixin ,
  • WANG Sheng ,
  • WU Weihua
Expand
  • Jiangxi Fisheries Research Institute, Nanchang 330039, China

Received date: 2019-11-19

  Online published: 2020-06-16

摘要

本试验旨在研究饥饿胁迫对彭泽鲫幼鱼形体指标、肌肉脂肪酸组成和肝脏脂蛋白脂酶基因表达的影响。选用初始体质量为(14.35±0.59) g的彭泽鲫180尾,随机分为3个组,每组3个重复,每个重复20尾鱼。设定3组试验鱼的饥饿时间分别为0(对照组)、14(S14组)和28 d (S28组)。结果表明:随着饥饿时间的延长,彭泽鲫幼鱼的体质量、肝体比(HSI)、脏体比(VSI)和肥满度(CF)均呈下降趋势,S28组幼鱼的体质量、HSI、VSI和CF显著低于对照组(P<0.05),S14组幼鱼的CF显著低于对照组(P<0.05)。S28组幼鱼的肌肉粗脂肪和粗蛋白质含量显著低于对照组(P<0.05)。S28组幼鱼肌肉中C16:0、C18:1n-9、总饱和脂肪酸(SFA)和总单不饱和脂肪酸(MUFA)相对含量显著低于对照组(P<0.05),C22:6n-3(DHA)、C20:4n-6(ARA)、总多不饱和脂肪酸(PUFA)和总高不饱和脂肪酸(HUFA)相对含量则显著高于对照组(P<0.05)。随着饥饿时间的延长,肝脏中脂蛋白脂酶(LPL) mRNA相对表达量呈先增后降的趋势,S14组显著高于对照组和S28组(P<0.05)。综上所述,饥饿胁迫使彭泽鲫幼鱼的体质量、HSI、VSI和CF均降低。饥饿过程中彭泽鲫幼鱼可同时消耗体内的脂肪和蛋白质维持代谢,以利用脂肪为主。饥饿期间彭泽鲫幼鱼主要利用SFA和MUFA氧化供能,对PUFA尤其是DHA和ARA则选择性保留。

本文引用格式

陈文静 , 丁立云 , 邓勇辉 , 肖俊 , 章海鑫 , 王生 , 巫伟华 . 饥饿胁迫对彭泽鲫幼鱼形体指标、肌肉脂肪酸组成和肝脏脂蛋白脂酶基因表达的影响[J]. 动物营养学报, 2020 , 32(6) : 2782 -2790 . DOI: 10.3969/j.issn.1006-267x.2020.06.038

Abstract

This experiment was conducted to evaluate the effects of starvation stress on physical indices, muscle fatty acid composition and liver lipoprotein lipase gene expression of juvenile crucian carp (Carassius auratus var. Pengze). A total of 180 Carassius auratus var. Pengze with an initial body weight of (14.35±0.59) g were randomly divided into 3 groups with 3 replicates per group and 20 fish per replicate. The starvation time of 3 groups was 0 (control group), 14 (S14 group) and 28 days (S28 group), respectively. The results showed that the body weight, hepatosomatic index, viserosomatic index and condition factor of juvenile Carassius auratus var. Pengze exhibited a declining trend with starvation time lengthening. Compared with the control group, the body weight, hepatosomatic index, viserosomatic index and condition factor in the S28 group were significantly decreased (P<0.05), and the condition factor in the S14 group was significantly decreased (P<0.05). The contents of crude fat and crude protein in muscle in the S28 group were significantly lower than those in the control group (P<0.05). Compared with the control group, the relative contents of C16:0, C18:1n-9, total saturated fatty acids (SFA) and total monounsaturated fatty acids (MUFA) in muscle were significantly decreased, while the relative contents of C22:6n-3 (DHA), C20:4n-6 (ARA), total polyunsaturated fatty acids (PUFA) and total highly unsaturated fatty acids (HUFA) were significantly increased in the S28 group (P<0.05). With the starvation time lengthening, the mRNA relative expression level of lipoprotein lipase gene in liver was firstly increased and then decreased. The mRNA relative expression level of lipoprotein lipase gene in the S14 group was significantly higher than that in the control group and S28 group (P<0.05). In conclusion, the body weight, hepatosomatic index, viserosomatic index and condition factor of juvenile Carassius auratus var. Pengze were decreased under starvation stress. Carassius auratus var. Pengze can consume both body fat and protein to maintain metabolism during starvation, mainly using fat. Carassius auratus var. Pengze gets energy by oxidation of SFA and MUFA mainly, while PUFA, especially DHA and ARA are selectively retained during starvation.

参考文献

[1] WANG T,HUNG C C Y,RANDALL D J.The comparative physiology of food deprivation:from feast to famine[J].Annual Review of Physiology,2006,68:223-251.
[2] ZENG L Q,LI F J,LI X M,et al.The effects of starvation on digestive tract function and structure in juvenile southern catfish (Silurus meridionalis Chen)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2012,162(3):200-211.  
[3] SUN Y F,WANG F,DONG S L.A comparative study of the effect of starvation regimes on the foraging behavior of Portunus trituberculatus and Charybdis japonica[J].Physiology & Behavior,2015,151:168-177.
[4] ANTONOPOULOU E,KENTEPOZIDOU E,FEIDANTSIS K,et al.Starvation and re-feeding affect Hsp expression,MAPK activation and antioxidant enzymes activity of European Sea Bass (Dicentrarchus labrax)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2013,165(1):79-88.  
[5] OH S Y,NOH C H,KANG R S,et al.Compensatory growth and body composition of juvenile black rockfish Sebastes schlegeli following feed deprivation[J].Fisheries Science,2008,74(4):846-852.  
[6] 覃川杰,邵婷,杨洁萍,等.饥饿胁迫对瓦氏黄颡鱼脂肪代谢的影响[J].水生生物学报,2015,39(1):58-65.
[7] WEN X B,CHEN L Q,KU Y M,et al.Effect of feeding and lack of food on the growth,gross biochemical and fatty acid composition of juvenile crab,Eriocheir sinensis[J].Aquaculture,2006,252(2/3/4):598-607.
[8] ZAMAL H,OLLEVIER F.Effect of feeding and lack of food on the growth,gross biochemical and fatty acid composition of juvenile catfish[J].Journal of Fish Biology,1995,46(3):404-414.  
[9] WATHNE E.Strategies for directing slaughter quality of farmed Atlantic salmon (Salmo salar) with emphasis on diet composition and fat deposition[M].Norges Landbruks Høgskole:[s.n.],1995.
[10] DE SILVA S S,GUNASEKERA R M,AUSTIN C M.Changes in the fatty acid profiles of hybrid red tilapia,Oreochromis mossambicus×O.niloticus,subjected to short-term starvation,and a comparison with changes in seawater raised fish[J].Aquaculture,1997,153(3/4):273-290.
[11] 柳敏海,罗海忠,傅荣兵,等.短期饥饿胁迫对鮸鱼生化组成、脂肪酸和氨基酸组成的影响[J].水生生物学报,2009,33(2):230-235.
[12] 丁立云,饶毅,陈文静,等.投喂频率对彭泽鲫幼鱼生长性能、形体指标和肌肉品质的影响[J].江苏农业科学,2017,45(19):228-231.
[13] AOAC.Official methods of analysis of AOAC international[S].20th ed. Rockville,MD:AOAC,2016.
[14] ZUO R T,AI Q H,MAI K S,et al.Effects of dietary n-3 highly unsaturated fatty acids on growth,nonspecific immunity,expression of some immune related genes and disease resistance of large yellow croaker (Larmichthys crocea) following natural infestation of parasites (Cryptocaryon irritans)[J].Fish & Shellfish Immunology,2012,32(2):249-258.  
[15] CHENG H L,WANG X,PENG Y X,et al.Molecular cloning and tissue distribution of lipoprotein lipase full-length cDNA from Pengze crucian carp (Carassius auratus var.Pengze)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2009,153(1):109-115.  
[16] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408.  
[17] NAVARRO I,GUTIÉRREZ J.Fasting and starvation[J].Biochemistry and Molecular Biology of Fishes,1995,4:393-434.
[18] LI H Y,XU W J,JIN J Y,et al.Effects of starvation on glucose and lipid metabolism in gibel carp (Carassius auratus gibelio,var.CAS Ⅲ)[J].Aquaculture,2018,496:166-175.
[19] 刘波,何庆国,唐永凯,等.饥饿胁迫对吉富罗非鱼生长及生理生化指标的影响[J].中国水产科学,2009,16(2):230-237.
[20] BOY C C,VANELLA F A,LATTUCA M E,et al.Effect of starvation on growth rate,muscle growth and energy density of puyen,Galaxias maculatus[J].Journal of Applied Ichthyology,2013,29(5):1001-1007.  
[21] TA?BOZAN O,EMRE Y,GÖKÇE M A,et al.The effects of different cycles of starvation and re-feeding on growth and body composition in rainbow trout (Oncorhynchus mykiss,Walbaum,1792)[J].Journal of Applied Ichthyology,2016,32(3):583-588.  
[22] 周凡,线婷,贝亦江,等.周期性"饥饿-再投喂"对大口黑鲈幼鱼补偿生长的影响[J].水产学杂志,2019,32(3):27-33.
[23] FOSTER A R,HOULIHAN D F,HALL S I.Effects of nutritional regime on correlates of growth rate in juvenile atlantic cod (Gadus morhua):comparison of morphological and biochemical measurements[J].Canadian Journal of Fisheries and Aquatic Sciences,1993,50(3):502-512.  
[24] 王腾飞,郭晓鸽,谷江稳,等.饥饿及过量投喂下银鲳幼鱼形态学指标及体成分变化[J].海洋学研究,2015,33(4):83-89.
[25] PERES H,SANTOS S,OLIVA-TELES A.Lack of compensatory growth response in gilthead seabream (Sparus aurata) juveniles following starvation and subsequent refeeding[J].Aquaculture,2011,318(3/4):384-388.
[26] 金鑫,徐钢春,杜富宽,等.饥饿胁迫对刀鲚形体、体成分及血液生化指标的影响[J].动物学杂志,2014,49(6):897-903.
[27] WANG Y,LI C,QIN J G,et al.Cyclical feed deprivation and refeeding fails to enhance compensatory growth in Nile tilapia,Oreochromis niloticus L[J].Aquaculture Research,2009,40(2):204-210.  
[28] BABAEI S,KENARI A A,HEDAYATI M,et al.Effect of diet composition on growth performance,hepatic metabolism and antioxidant activities in Siberian sturgeon (Acipenser baerii,Brandt,1869) submitted to starvation and refeeding[J].Fish Physiology and Biochemistry,2016,42(6):1509-1520.  
[29] EINEN O,WAAGAN B,THOMASSEN M S.Starvation prior to slaughter in Atlantic salmon (Salmo salar):Ⅰ.Effects on weight loss,body shape,slaughter-and fillet-yield,proximate and fatty acid composition[J].Aquaculture,1998,166(1/2):85-104.
[30] 周爱国,茅沈丽,王超,等.饥饿胁迫对杂交鳢生长及生化组成的影响[J].水生态学杂志,2012,33(5):78-82.
[31] HUNG S S O,LIU W,LI H B,et al.Effect of starvation on some morphological and biochemical parameters in white sturgeon,Acipenser transmontanus[J].Aquaculture,1997,151(1/2/3/4):357-363.
[32] HALVER J E.Fish nutrition[M].2nd ed.New York:Academic Press,1989:239-345.
[33] 谢小军,邓利,张波.饥饿对鱼类生理生态学影响的研究进展[J].水生生物学报,1998,22(2):181-188.
[34] SARGENT J R,TOCHER D R,BELL J G.The lipids[M]//HALVER J E,HARDY R W.Fish nutrition.San Diego,CA,USA:Academic Press,2002:181-257.
[35] SIDELL B D,CROCKETT E L,DRIEDZIC W R.Antarctic fish tissues preferentially catabolize monoenoic fatty acids[J].Journal of Experimental Zoology,1995,271(2):73-81.  
[36] MCKENZIE D J,HIGGS D A,DOSANJH B S,et al.Dietary fatty acid composition influences swimming performance in Atlantic salmon (Salmo salar) in seawater[J].Fish Physiology and Biochemistry,1998,19(2):111-122.  
[37] MOURENTE G,TOCHER D R.Effects of weaning onto a pelleted diet on docosahexaenoic acid (22∶6n-3) levels in brain of developing turbot (Scophthalmus maximus L.)[J].Aquaculture,1992,105(3/4):363-377.
[38] SARGENT J R,BELL J G,BELL M V,et al.Requirement criteria for essential fatty acids[J].Journal of Applied Ichthyology,1995,11(3/4):183-198.
[39] ZABELINSKII S A,CHEBOTAREVA M A,KOSTKIN V B,et al.Phospholipids and their fatty acids in mitochondria,synaptosomes and myelin from the liver and brain of trout and rat:a new view on the role of fatty acids in membranes[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1999,124(2):187-193.  
[40] 刘峰,吕小康,刘阳阳,等.饥饿对大黄鱼幼鱼肌肉中氨基酸和脂肪酸组成的影响[J].渔业科学进展,2018,39(5):58-65.
[41] LIAO K,MENG R,RAN Z S,et al.Short-term starvation in silver pomfret (Pampus argenteus):molecular effects on lipid mobilization and utilization[J].Aquaculture Research,2017,48(9):4874-4885.  
[42] 刘建业,陈华谱,江东能,等.饥饿及复投喂对金钱鱼肠型脂肪酸结合蛋白基因表达的影响[J].水生生物学报,2019,43(4):701-707.
[43] ZAPPATERRA M,DESERTI M,MAZZA R,et al.A gene and protein expression study on four porcine genes related to intramuscular fat deposition[J].Meat Science,2016,121:27-32.
[44] GELDENHUYS W J,LIN L,DARVESH A S,et al.Emerging strategies of targeting lipoprotein lipase for metabolic and cardiovascular diseases[J].Drug Discovery Today,2017,22(2):352-365.  
[45] WARNKE I,GORALCZYK R,FUHRER E,et al.Dietary constituents reduce lipid accumulation in murine C3H10 T1/2 adipocytes:a novel fluorescent method to quantify fat droplets[J].Nutrition & Metabolism,2011,8(1):30.
[46] WANG H,ECKEL R H.Lipoprotein lipase:from gene to obesity[J].American Journal of Physiology:Endocrinology and Metabolism,2009,297(2):E271-E288.
[47] HUANG H L,ZHANG Y,CAO M Y,et al.Effects of fasting on the activities and mRNA expression levels of lipoprotein lipase (LPL),hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) in spotted seabass Lateolabrax maculatus[J].Fish Physiology and Biochemistry,2018,44(1):387-400.  
[48] 张斌,李福昌,王诚,等.短期限饲对家兔肝脏和骨骼肌脂质代谢的影响[J].动物营养学报,2019,31(1):243-250.
[49] 王贞杰,叶保民,常青,等.维生素C对圆斑星鲽早期发育的影响[J].渔业科学进展,2018,39(2):96-103.
文章导航

/