本试验旨在研究饲粮中添加枯草芽孢杆菌和复合酶制剂对獭兔生长性能、养分表观消化率、屠宰性能、免疫器官指数及毛皮质量的影响。采用单因子设计,选取体重相近的健康42日龄獭兔126只,随机分为3组,每组42个重复,每个重复1只兔。对照组饲喂基础饲粮+300 mg/kg金霉素,试验Ⅰ组饲喂基础饲粮+200 mg/kg枯草芽孢杆菌+200 mg/kg复合酶制剂,试验Ⅱ组饲喂基础饲粮+400 mg/kg枯草芽孢杆菌+400 mg/kg复合酶制剂。预试期5 d,正试期41 d。结果表明:1)各组之间獭兔的末重、平均日采食量、平均日增重、料重比、腹泻率和死亡率无显著差异(P>0.05),腹泻率和死亡率均以试验Ⅰ组最低。2)对照组獭兔的粗纤维表观消化率极显著高于试验Ⅱ组(P<0.05),对照组和试验Ⅱ组獭兔的钙表观消化率极显著高于试验Ⅰ组(P<0.01)。各组之间獭兔的干物质、粗蛋白质、粗灰分、磷、中性洗涤纤维和酸性洗涤纤维表观消化率无显著差异(P>0.05)。3)各组之间獭兔的宰前活重、胴体重、半净膛率和全净膛率无显著差异(P>0.05)。4)各组之间獭兔的胸腺指数、脾脏指数和圆小囊指数无显著差异(P>0.05)。5)对照组和试验Ⅰ组獭兔的臀部被毛密度极显著高于试验Ⅱ组(P<0.01),对照组和试验Ⅰ组獭兔的肩部被毛密度显著高于试验Ⅱ组(P<0.05);对照组獭兔的腹部被毛密度极显著低于试验Ⅰ组和试验Ⅱ组(P<0.01),试验Ⅰ组极显著低于试验Ⅱ组(P<0.01)。对照组獭兔的臀部皮张厚度极显著低于试验Ⅱ组(P<0.01)。由此可见,饲粮中添加枯草芽孢杆菌和复合酶制剂对獭兔生长性能、屠宰性能、免疫器官指数无显著影响,对粗纤维和钙表观消化率及被毛密度、臀部皮张厚度有显著影响。综合考虑以上指标,獭兔饲粮中添加200 mg/kg枯草芽孢杆菌+200 mg/kg复合酶制剂效果较好。
This experiment was conducted to study the effects of dietary Bacillus subtilis and complex enzyme preparation on growth performance, nutrient apparent digestibilities, slaughter performance, immune organ indexes and fur quality of Rex rabbits. A single factor design was adopted, a total of 126 healthy 42-day-old Rex rabbits with similar body weight were randomly divided into 3 groups with 42 replicates per group and 1 rabbit per replicate. Rabbits in the control group were fed a basal diet+300 mg/kg aureomycin, rabbits in the experimental group Ⅰ were fed a basal diet+200 mg/kg Bacillus subtilis+200 mg/kg complex enzyme preparation, and rabbits in the experimental group Ⅱ were fed a basal diet+400 mg/kg Bacillus subtilis+400 mg/kg complex enzyme preparation. The pre-experimental period lasted for 5 days, and the experimental period lasted for 41 days. The results showed as follows: 1) there were no significant effects on the final weight, average daily feed intake, average daily gain, feed to gain ratio, diarrhea rate and mortality rate of Rex rabbits among all groups (P>0.05), and the diarrhea rate and mortality rate were the lowest in experimental group Ⅰ. 2) The crude fiber apparent digestibility of Rex rabbits of control group was significantly higher than that of experimental group Ⅱ (P<0.01), and the calcium apparent digestibility of Rex rabbits of control group and experimental group Ⅱ was significantly higher than that of experimental group Ⅰ (P<0.01). There were no significant effects on the apparent digestibilities of dry matter, crude protein, crude ash, phosphorus, neutral detergent fiber and acid detergent fiber of Rex rabbits among all groups (P>0.05). 3) There were no significant effects on the live weight before slaughter, carcass weight, semi-eviscerated ratio and eviscerated ratio of Rex rabbits among all groups (P>0.05). 4) There were no significant effects on the thymus index, spleen index and sacculus rotundus index of Rex rabbits among all groups (P>0.05). 5) The hair density in buttocks of Rex rabbits of control group and experimental group Ⅰ was significantly higher than that of experimental group Ⅱ (P<0.01), the hair density in shoulder of Rex rabbits of control group and experimental group Ⅰ was significantly higher than that of experimental group Ⅱ (P<0.05); the hair density in belly of Rex rabbits of control group was significantly lower than that of experimental group Ⅰ and experimental group Ⅱ (P<0.01), and experimental group Ⅰ was significantly lower than experimental group Ⅱ (P<0.01). The pelt thickness in buttocks of Rex rabbits of control group was significantly lower than that of experimental group Ⅱ (P<0.01). In conclusion, dietary Bacillus subtilis and complex enzyme preparation have no significant effects on growth performance, slaughter performance and immune organ indexes of Rex rabbits, but have significant effects on apparent digestibilities of crude fiber and calcium and hair density and pelt thickness in buttocks. Considering the above indices, dietary supplemented 200 mg/kg Bacillus subtilis+200 mg/kg complex enzyme preparation for Rex rabbits has better effects.
[1] 王清吉,王世成,唐世洪,等.添加粗酶制剂对法系獭兔生产性能的影响[J].中国养兔杂志,2001(2):5-6.
[2] 谷子林,秦应和,任克良.中国养兔学[M].北京:中国农业出版社,2013.
[3] 谷子林.断乳仔兔腹泻发生机制及生物活性物质的调控研究[D].博士学位论文.哈尔滨:东北农业大学,2004.
[4] 谷子林,顾时贵,任文社,等.力克斯兔被毛密度研究[J].中国养兔杂志,1999(4):18-21.
[5] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.
[6] 张南斌,孙瑛超,赵晓宇,等.饲粮不同粗纤维水平对生长肉兔生长性能、屠宰性能、肌肉品质及胃肠道和免疫器官发育的影响[J].动物营养学报,2018,30(5):1789-1797.
[7] 杨丽杰,李新民,谷子林,等.不同营养类型日粮添加外源酶制剂对生长獭兔生产性能的影响[J].河北农业大学学报,2004,27(3):93-96.
[8] 郭肖兰.复合酶、益生素、纳米硒、维生素E对断奶獭兔生长性能及免疫的影响[D].硕士学位论文.杨凌:西北农林科技大学,2011.
[9] 蔡海路,苏加义.酶制剂对獭兔生产性能影响的研究[J].安徽农学通报,2007,13(12):152-153.
[10] 张福勋,梁丽霞.獭兔日粮中添加酶制剂的效果[J].当代畜牧,2004(2):35-36.
[11] 邵乐,潘孝青,李强,等.非粮型日粮中添加饲用酶制剂对5月龄獭兔生产性能及营养物质消化的影响[J].江苏农业科学,2016,44(9):267-269.
[12] 武静龙.复合酶制剂对獭兔生产性能和血清生理生化指标的影响[D].硕士学位论文.长沙:湖南农业大学,2006.
[13] 史钧,徐汉涛.酶制剂对肉兔生长性能和消化机能的影响[J].中国养兔杂志,2001(3):20-23.
[14] 康健,吴娟.复合酶制剂对断乳肉兔生长性能的影响[J].安徽技术师范学院学报,2003,17(3):217-219.
[15] 李建民,张光勤,侯玉泽.日粮中添加饲用酶制剂对肉兔生长性能的影响[J].黑龙江畜牧兽医,2005(12):72-73.
[16] 韩正康.大麦日粮添加酶制剂影响家禽营养生理及改善生产性能的研究[J].畜牧与兽医,2000,32(1):1-4.
[17] 任永军,雷岷,邝良德.复合芽孢杆菌制剂对肉兔生产性能、免疫器官指数及肉质的影响[J].中国畜牧杂志,2014,50(3):73-77.
[18] JØRGENSEN J N,LAGUNA J S,MILLÁN C,et al.Effects of a Bacillus-based probiotic and dietary energy content on the performance and nutrient digestibility of wean to finish pigs[J].Animal Feed Science and Technology,2016,221:54-61.
[19] WANG H,KIM KP,KIM I H.Influence of Bacillus subtilis GCB-13-001 on growth performance,nutrient digestibility,blood characteristics,faecal microbiota and faecal score in weanling pigs[J].Journal of Animal Physiology and Animal Nutrition,2019,103(11/12):1919-1925.
[20] KIM K,HE Y J,XIOMG X,et al.Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E.coli[J].Journal of Animal Science and Biotechnology,2019,10:52,doi:10.1186/s40104-019-0364-3.
[21] 朱瑾,匡佑华,陈继发,等.枯草芽孢杆菌对肥育猪生长性能、肉品质和抗氧化能力的影响[J].动物营养学报,2019,31(6):2572-2578.
[22] 宋强.芽孢杆菌制剂对肉兔生产性能的影响[D].硕士学位论文.成都:四川农业大学,2015.
[23] 张爱武,董斌,左璐雅,等.不同水平枯草芽孢杆菌对鹌鹑内脏器官及小肠发育的影响[J].经济动物学报,2010,14(2):98-101.
[24] 张学科.刍议枯草芽孢杆菌在动物饲料中的应用[J].畜牧兽医科技信息,2019(6):161.
[25] 淡瑞芳,张海涛.酶制剂在国内兔营养中的作用及机理的研究进展[J].中国养兔,2012(5):23-26.
[26] 谷子林.酶制剂对育肥兔饲料利用率的影响[J].河北科技报,2013(8):1.
[27] 高振华,何瑞国,谷子林,等.日粮中添加复合酶制剂对不同日龄獭兔生产性能及营养物质消化率的影响[J].黑龙江畜牧兽医,2006(9):102-103.
[28] 高振华,张宝庆,赵博伟,等.獭兔日粮中添加酶制剂的效果[J].中国养兔杂志,1998(6):19-23.