动物营养学报 >

2020 , Vol. 32 >Issue 6: 2861 - 2868

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.046

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

羊源解淀粉芽孢杆菌fsznc-06对小鼠生长和免疫功能的影响

  • 张楠驰 ,
  • 李娟 ,
  • 王利 ,
  • 魏勇
展开
  • 1. 西南民族大学, 青藏高原动物遗传资源保护与利用教育部和四川省重点实验室, 成都 610041;
    2. 四川省畜牧科学研究院, 动物遗传育种四川省重点实验室, 成都 610066
张楠驰(1993-),男,辽宁抚顺人,硕士研究生,研究方向为动物免疫学。E-mail:zhangnanchi@126.com

收稿日期: 2019-12-08

  网络出版日期: 2020-06-16

基金资助

四川省肉羊创新团队防疫岗位；农业农村部农业重大技术协同推广计划试点四川省肉羊高效生产配套技术推广应用

收起

Effects of Bacillus amyloliquefaciens fsznc-06 Isolated from Goats on Growth and Immune Function of Mice

  • ZHANG Nanchi ,
  • LI Juan ,
  • WANG Li ,
  • WEI Yong
Expand
  • 1. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610041, China;
    2. Animal Genetics and Breeding Key Laboratory of Sichuan Province, Animal Science Academy of Sichuan Province, Chengdu 610066, China

Received date: 2019-12-08

  Online published: 2020-06-16

Fold

摘要

本试验旨在探究羊源解淀粉芽孢杆菌fsznc-06对小鼠生长和免疫功能的影响。选取36只健康的小鼠，随机分为3组，每组3个重复，每个重复4只。低剂量组（JD1组）和高剂量组（JD2组）每2 d灌胃1次浓度分别为1×108和1×1010 CFU/mL的羊源解淀粉芽孢杆菌fsznc-06菌悬液，灌胃量为0.3 mL/只；对照组（DZ组）每2 d灌胃1次0.9%生理盐水，灌胃量为0.3 mL/只。试验期为28 d。测定各组小鼠生长性能、血清生化指标、生长和免疫相关基因表达量以及肠道菌群多样性。结果表明：1）与DZ组相比，JD1组第7、14、21和28天体增重显著或极显著提高（P<0.05或P<0.01），JD2组第7和14天体增重显著或极显著提高（P<0.05或P<0.01）。与DZ组相比，JD1组第1~7天、第8~14天、第15~21天和第22~28天料重比显著或极显著降低（P<0.05或P<0.01），JD2组第1~7天、第15~21天和第22~28天料重比显著或极显著降低（P<0.05或P<0.01）。2）与DZ组相比，JD1组心脏、小肠指数以及JD2组心脏、脾脏、胸腺、大肠指数显著或极显著提高（P<0.05或P<0.01），JD1组血清总超氧化物歧化酶（T-SOD）、碱性磷酸酶（AKP）活性以及JD2组血清AKP活性显著或极显著提高（P<0.05或P<0.01）。3）与DZ组相比，JD1组脾脏胰岛素样生长因子-1（IGF-1）基因表达量和JD2组脾脏IGF-1和干扰素α11（IFNα11）基因表达量显著或极显著提高（P<0.05或P<0.01），JD1组脾脏白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和JD2组IL-1β、白细胞介素-6（IL-6）、一氧化氮合酶（iNOS）和TNF-α基因表达量显著或极显著降低（P<0.05或P<0.01）。4）与DZ组相比，JD1和JD2组小鼠肠道菌群多样性和双歧杆菌属的相对丰度均明显提高，优势物种相对丰度也明显提高。由此可见，羊源解淀粉芽孢杆菌fsznc-06可促进小鼠生长，提高免疫功能，且JD2组效果相对更佳。

关键词： 解淀粉芽孢杆菌; 小鼠; 生长; 免疫; 肠道菌群

本文引用格式

张楠驰 , 李娟 , 王利 , 魏勇 . 羊源解淀粉芽孢杆菌fsznc-06对小鼠生长和免疫功能的影响[J]. 动物营养学报, 2020 , 32(6) : 2861 -2868 . DOI: 10.3969/j.issn.1006-267x.2020.06.046

Abstract

This experiment was conducted to investigate the effects of Bacillus amyloliquefaciens fsznc-06 isolated from goats on growth and immune function of mice. Thirty-six healthy mice were randomly divided into 3 groups with 3 replicates per group and 4 mice per replicate. Mice in low dose group (JD1 group) and high dose group (JD2 group) were given 1×108 and 1×1010 CFU/mL Bacillus amyloliquefaciens fsznc-06 isolated from goats by gavage once every 2 days, and the gavage volume was 0.3 mL per mouse; mice in the control group (DZ group) were given 0.9% normal saline by gavage once every 2 days, and the gavage volume was 0.3 mL per mouse. The experiment lasted for 28 days. The growth performance, serum biochemical index, expression levels of growth and immune related genes and intestinal flora diversity of mice were measured. The results showed as follows: 1) compared with the DZ group, the body weight gain on days 7, 14, 21 and 28 of JD1 group was significantly increased (P<0.05 or P<0.01), and the body weight gain on days 7 and 14 of JD2 group was significantly increased (P<0.05 or P<0.01). Compared with the DZ group, the feed to gain ratio on days 1 to 7, days 8 to 14, days 15 to 21 and days 22 to 28 of JD1 group was significantly decreased (P<0.05 or P<0.01), and the feed to gain ratio on days 1 to 7, days 15 to 21 and days 22 to 28 of JD2 group was significantly decreased (P<0.05 or P<0.01). 2) Compared with the DZ group, the indexes of heart and small intestine of JD1 group and indexes of heart, spleen, thymus and large intestine of JD2 group were significantly increased (P<0.05 or P<0.01), the activities of total superoxide dismutase (T-SOD), alkaline phosphatase (AKP) in serum of JD1 group and serum AKP activity of JD2 group were significantly increased (P<0.05 or P<0.01). 3) Compared with the DZ group, the spleen insulin-like growth factor-1 (IGF-1) gene expression level of JD1 group and expression levels of IGF-1 and interferon alpha 11 (IFNα11) in spleen of JD2 group were significantly increased (P<0.05 or P<0.01), and the expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) in spleen of JD1 group and expression levels of IL-1β, interleukin-6 (IL-6), nitric oxide synthase (iNOS) and TNF-α in spleen of JD2 group were significantly decreased (P<0.05 or P<0.01). 4) Compared with the DZ group, the intestinal flora diversity and relative abundance of Bifidobacteria of JD1 and JD2 groups were increased, and the relative abundance of dominant species was also increased. In conclusion, Bacillus amyloliquefaciens fsznc-06 isolated from goats can promote the growth and improve the immune funtion of mice, and JD2 group has a relatively better effect.

Key words： Bacillus amyloliquefaciens; mice; growth; immune; intestinal flora

参考文献

[1] 李晓宇.解淀粉芽孢杆菌LXY-6-2筛选、鉴定、诱变与发酵条件优化及其对辣椒根腐病的生物防治研究[D].硕士学位论文.咸阳:西北农林科技大学,2018.
[2] 孙淑琴,杨秀荣,孙冰冰,等.解淀粉芽孢杆菌Bs-18生防功能相关基因的初步鉴定[J].山东农业科学,2019,51(3):13-15,23.
[3] 张彤.两株鹿源芽孢杆菌的分离鉴定及生物学特性研究[D].硕士学位论文.沈阳:沈阳农业大学,2017.
[4] CAO G T,ZHAN X A,ZHANG L L,et al.Modulation of broilers' caecal microflora and metabolites in response to a potential probiotic Bacillus amyloliquefaciens[J].Journal of Animal Physiology and Animal Nutrition,2018,102(2):e909-e917.
[5] BINDIYA E S,TINA K J,SASIDHARAN R S,et al.BaCf3:highly thermostable bacteriocin from Bacillus amyloliquefaciens BTSS3 antagonistic on food-borne pathogens[J].3 Biotech,2019,9(4):136.
[6] 姚佳明,田亚平.解淀粉芽孢杆菌抑菌肽的分离鉴定及其抑菌谱表征[J/OL].食品科学,2019:1-12[2019-10-24].http://kns.cnki.net/kcms/detail/11.2206.TS.20190827.0912.002.html.
[7] 熊美玲.沸石粉基芽孢杆菌剂对污水处理的研究[D].硕士学位论文.哈尔滨:哈尔滨工业大学,2017.
[8] 柴庆凯,张斌,常若葵,等.解淀粉芽孢杆菌LJ02对黄瓜抗灰霉病菌的生防效果及其诱导抗性机理的初步研究[J].植物病理学报,2019,49(6):825-835.
[9] 杨乔乔,安贤惠,韩迎亚,等.大鳞副泥鳅腐皮病病原菌及其拮抗菌的分离筛选[J].水产科学,2019,38(5):666-675.
[10] 宁豫昌,张晓静,乔宏兴.猪肠道中产蛋白酶解淀粉芽孢杆菌的筛选、鉴定及其酶学性质的初步研究[J].中国畜牧兽医,2017,44(8):2398-2407.
[11] 程英显.解淀粉芽孢杆菌TL对肉鸡血清代谢组学影响[D].硕士学位论文.武汉:华中农业大学,2018.
[12] 朱博.解淀粉芽孢杆菌对仔猪生长性能、抗氧化能力、免疫功能和肠道菌群的影响[D].硕士学位论文.南京:南京农业大学,2016.
[13] 郭政宏.藏猪源益生芽孢杆菌的分离鉴定及候选益生菌株的筛选[D].硕士学位论文.成都:西南民族大学,2016.
[14] 王岩,陈楠楠,侯美如,等.解淀粉芽孢杆菌SSY1株的安全性试验[J].中国畜牧兽医,2017,44(3):928-934.
[15] 张李荣.三种益生菌对雏鸡生长、小肠发育及盲肠微生物区系的影响[D].硕士学位论文.扬州:扬州大学,2018.
[16] 徐毓琴.产γ-氨基丁酸益生菌的筛选及对小鼠微生态效应的影响研究[D].硕士学位论文.雅安:四川农业大学,2016.
[17] 李凤格.分枝杆菌LpqT蛋白可抑制巨噬细胞炎性因子的表达及凋亡以促进细菌在体内生存[D].硕士学位论文.苏州:苏州大学,2017.
[18] 张雪.一株解淀粉芽孢杆菌的特性及抑菌活性研究[D].硕士学位论文.大连:大连工业大学,2018.
[19] 夏京津,陈建武,宋怿,等.解淀粉芽孢杆菌HE活性成分鉴定及抗菌特性分析[J].南方水产科学,2019,15(3):41-49.
[20] 周银丽,郭建伟,杨伟,等.石榴枯萎病菌拮抗菌B110的分离鉴定[J].中国南方果树,2018,47(6):1-6.
[21] 王静,宁燕夏,糟雪云,等.解淀粉芽孢杆菌B6防治西瓜枯萎病及对西瓜幼苗抗氧化酶的诱导作用[J].中国植保导刊,2018,38(6):5-10.
[22] 覃初斌,朱海燕,梅小强,等.解淀粉芽孢杆菌对黑鲷幼鱼生长和肝脏功能的影响[J].中国农业科技导报,2017,19(12):110-118.
[23] 杨敏馨.解淀粉芽孢杆菌替代饲用抗生素在肉鸡上的应用效果研究[D].硕士学位论文.南京:南京农业大学,2016.
[24] 李仕成.拮抗罗非鱼无乳链球菌的芽孢杆菌筛选与研究[D].硕士学位论文.长沙:湖南农业大学,2016.
[25] 徐函,曹雪芳,刘容容,等.解淀粉芽孢杆菌SC06与肠毒性大肠杆菌K88对IPEC-1细胞转运载体、紧密连接、凋亡和免疫相关基因表达的影响[J].动物营养学报,2019,31(5):2267-2277.
[26] 王阳.解淀粉芽孢杆菌对动物抗氧化功能的影响及其作用机制的研究[D].博士学位论文.杭州:浙江大学,2018.
[27] 汤红燕,肖斌,王利平,等.胰岛素样生长因子家族与皮肤病[J].中国医学科学院学报,2019,41(3):415-418.
[28] 李红伟,钟淡龙,陈圆,等.惠阳胡须鸡IGF-Ⅰ和GHRL基因多态性研究[J].河南农业科学,2017,46(9):152-155.
[29] 朱菡,姚颖.肠道菌群及益生菌干预:慢性肾脏病治疗的新视角[J].科学通报,2019,64(3):291-297.
[30] DE OLIVEIRA M J K,SAKOMURA N K,DE PAULA DORIGAM J C,et al.Bacillus amyloliquefaciens CECT 5940 alone or in combination with antibiotic growth promoters improves performance in broilers under enteric pathogen challenge[J].Poultry Science,2019,98(10):4391-4400.  
[31] HÜTT P,SHCHEPETOVA J,LÕIVUKENE K,et al.Antagonistic activity of probiotic Lactobacilli and Bifidobacteria against entero- and uropathogens[J].Journal of Applied Microbiology,2006,100(6):1324-1332.  
[32] 堵雅芳,石羽杰,刘彪,等.乳双歧杆菌HN019与健康[J].卫生研究,2019,48(5):850-852.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2