动物营养学报 >

2020 , Vol. 32 >Issue 6: 2904 - 2910

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.051

研究简报 SHORT COMMUNICATION

酵母β-葡聚糖对脂多糖诱导的绵羊淋巴细胞氧化应激损伤的保护作用

  • 于春微 ,
  • 李冬芳 ,
  • 刘世雄 ,
  • 郝凌魁 ,
  • 高民 ,
  • 胡红莲 ,
  • 刘大程
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  • 1. 内蒙古农业大学兽医学院, 呼和浩特 010018;
    2. 内蒙古农牧业科学院动物营养与饲料研究所, 呼和浩特 010031
于春微(1987-),女,内蒙古赤峰人,博士研究生,从事兽医微生物学与免疫学研究。E-mail:yuchunweicf@163.com

收稿日期: 2019-12-11

  网络出版日期: 2020-06-16

基金资助

国家自然科学基金项目（31260560）；现代农业（奶牛）产业技术体系建设专项（CARS-36）

收起

Protective Effects of β-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte

  • YU Chunwei ,
  • LI Dongfang ,
  • LIU Shixiong ,
  • HAO Lingkui ,
  • GAO Min ,
  • HU Honglian ,
  • LIU Dacheng
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  • 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Research Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Animal Sciences, Hohhot 010031, China

Received date: 2019-12-11

  Online published: 2020-06-16

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摘要

本试验旨在研究酵母β-葡聚糖对绵羊淋巴细胞抗氧化能力的影响。在建立体外脂多糖（LPS）诱导绵羊淋巴细胞氧化应激模型的基础上，通过在培养液中添加不同浓度[0（对照）、5、10、20、50、100 μg/mL]的酵母β-葡聚糖，运用四甲基偶氮唑盐（MTT）比色法测定酵母β-葡聚糖对细胞活性的影响，用生物化学法测定酵母β-葡聚糖对绵羊淋巴细胞抗氧化指标变化的影响。结果表明：0~20 μg/mL酵母β-葡聚糖作用于淋巴细胞，细胞存活率无显著变化（P>0.05），当使用50 μg/mL酵母β-葡聚糖处理淋巴细胞后，细胞存活率显著降低（P<0.05）；在LPS作用于淋巴细胞后，与对照组相比较，丙二醛（MDA）含量极显著增多（P<0.01），超氧化物歧化酶（SOD）和氧化氢酶（CAT）活性显著下降（P<0.05），谷胱甘肽过氧化物酶（GSH-Px）活性极显著下降（P<0.01）；添加10 μg/mL酵母β-葡聚糖作用6 h后，与LPS组相比较，淋巴细胞内SOD和CAT活性极显著增强（P<0.01），GSH-Px活性显著增强（P<0.05），MDA和ROS含量极显著降低（P<0.01）。综上，LPS可诱导绵羊淋巴细胞氧化应激，酵母β-葡聚糖可抑制LPS诱导的淋巴细胞氧化损伤，对LPS诱导的绵羊淋巴细胞氧化损伤有良好的保护作用，存在剂量依赖效应。

关键词： 酵母&beta; -葡聚糖; 淋巴细胞; 脂多糖; 活性氧; 抗氧化

本文引用格式

于春微 , 李冬芳 , 刘世雄 , 郝凌魁 , 高民 , 胡红莲 , 刘大程 . 酵母β-葡聚糖对脂多糖诱导的绵羊淋巴细胞氧化应激损伤的保护作用[J]. 动物营养学报, 2020 , 32(6) : 2904 -2910 . DOI: 10.3969/j.issn.1006-267x.2020.06.051

Abstract

This experiment was conducted to study the effects of β-glucan from Saccharomyces cerevisiae on the antioxidant capacity of sheep lymphocytes. Based on the establishment of lipopolysaccharide (LPS)-induced oxidative stress in sheep lymphocytes, the effects of β-glucan on cell viability were determined by MTT assay by adding different concentrations [0(control), 5, 10, 20, 50 and 100 μg/mL] of β-glucan to the culture medium. The biochemical method was used to determine the effects of β-glucan on the changes of antioxidant indexes in sheep lymphocytes. The results showed as follows: β-glucan showed no cytotoxicity after being applied to lymphocytes at a concentration of 0 to 20 μg/mL, when the concentration of β-glucan was 50 μg/mL, the cell viability decreased significantly (P<0.05); compared with the control group, the malonaldehyde (MDA) content extremely significantly increased (P<0.01), superoxide dismutase (ROS) and catalase (CAT) activities extremely significantly decreased (P<0.05), and glutathion peroxidase (GSH-Px) activity decreased extremely significantly (P<0.01) in lymphocytes treated by LPS. After adding 10 μg/mL β-glucan for 6 h, compared with the LPS group, the activity of SOD and CAT in lymphocytes extremely significantly increased (P<0.01), the activity of GSH-Px significantly increased (P<0.05), and the contents of MDA and ROS extremely significantly decreased (P<0.01). In conclusions, LPS can induce oxidative stress in sheep lymphocytes. β-glucan can inhibit LPS-induced oxidative injury of lymphocytes and has a good protective effect on LPS-induced oxidative injury of sheep lymphocytes, and there is a dose-dependent effect.

Key words： yeast β-glucan; lymphocyte; lipopolysaccharide; reactive oxygen species; antioxidant

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