动物营养学报 >

2020 , Vol. 32 >Issue 7: 3089 - 3098

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.07.018

猪营养与饲料 SWINE NUTRITION AND FEED

乳铁蛋白对大河乌猪繁殖性能及母猪与仔猪铁营养、血细胞参数、免疫指标和猪β防御素基因表达的影响

  • 车丽涛 ,
  • 李岑曦 ,
  • 徐娜娜 ,
  • 张春勇 ,
  • 刘水灵 ,
  • 安清聪 ,
  • 郭荣富
展开
  • 云南农业大学, 云南省动物营养与饲料重点实验室, 昆明 650201
车丽涛(1979-),女,云南昆明人,博士研究生,从事猪的营养与免疫研究。E-mail:272320726@qq.com

收稿日期: 2020-01-22

  网络出版日期: 2020-07-15

基金资助

云南省科技厅重大科技专项子课题"种猪生产营养饲料技术集成与示范"（2012ZAO18-3）与"乳铁蛋白对新生期仔猪肠道健康的影响"（A3007495）

收起

Effects of Lactoferrin on Reproductive Performance of Dahe Black Sows and Iron Nutrition, Blood Cell Parameters, Immune Indexes and Porcine β-Defensin Gene Expression of Sows and Piglets

  • CHE Litao ,
  • LI Cenxi ,
  • XU Nana ,
  • ZHANG Chunyong ,
  • LIU Shuiling ,
  • AN Qingcong ,
  • GUO Rongfu
Expand
  • Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China

Received date: 2020-01-22

  Online published: 2020-07-15

Fold

摘要

本试验旨在研究乳铁蛋白（LF）对大河乌猪繁殖性能及母猪与仔猪铁营养、血细胞参数、免疫指标和猪β防御素（pBD）基因表达的影响。选用经产（3~4胎）、产仔数和体重相近的大河乌猪母猪60头，随机分为5组，每组12个重复，每重复1头母猪。从妊娠第80天至分娩，对照组饲喂基础饲粮，LF1组、LF2组、LF3组分别在基础饲粮中添加100、200、300 mg/kg LF，甘氨酸螯合铁（Fe-Gly）组在基础饲粮中添加500 mg/kg Fe-Gly。考察母猪繁殖性能，检测母猪和仔猪血液红细胞计数（RBC）、血红蛋白（HGB）含量、血清免疫指标以及母猪血清和乳汁铁含量，并检测仔猪肝脏、脾脏和十二指肠pBD基因表达量。结果表明：1）与对照组相比，LF2组仔猪初生重及初生窝重显著提高（P<0.05），LF1和LF3组仔猪初生窝重显著提高（P<0.05）。2）与对照组相比，LF2、LF3和Fe-Gly组母猪血液RBC及HGB含量显著提高（P<0.05），LF1、LF2、LF3和Fe-Gly组仔猪血液RBC及HGB含量显著提高（P<0.05）。3）与对照组相比，LF1、LF2和LF3组母猪血清和乳汁铁含量显著提高（P<0.05）。4）与对照组相比，LF1、LF2和LF3和Fe-Gly组仔猪血清乳酸脱氢酶（LDH）活性显著降低（P<0.05），血清一氧化氮合酶（NOS）活性显著提高（P<0.05）；LF1、LF2组仔猪血清免疫球蛋白G（IgG）含量和溶菌酶（LSZ）活性显著提高（P<0.05）。5）与对照组相比，LF2组仔猪肝脏、脾脏、十二指肠pBD-1基因，肝脏、脾脏pBD-2基因以及脾脏pBD-3基因表达量显著提高（P<0.05）；LF1组仔猪肝脏pBD-1、pBD-2基因以及LF3组仔猪肝脏、脾脏pBD-1基因表达量显著提高（P<0.05）；LF1、LF2、LF3和Fe-Gly组仔猪十二指肠pBD-2基因以及肝脏、十二指肠pBD-3基因表达量显著降低（P<0.05）。由此可见，在大河乌猪妊娠后期饲粮中添加适宜剂量（200 mg/kg）LF可提高仔猪初生重及初生窝重，改善仔猪铁营养和提高血清免疫指标，增加仔猪肝脏、脾脏和十二指肠pBD-1基因表达量，有利于提高仔猪的抗病能力。

关键词： 乳铁蛋白; 大河乌猪; 繁殖性能; 血红蛋白; 猪&beta; 防御素

本文引用格式

车丽涛 , 李岑曦 , 徐娜娜 , 张春勇 , 刘水灵 , 安清聪 , 郭荣富 . 乳铁蛋白对大河乌猪繁殖性能及母猪与仔猪铁营养、血细胞参数、免疫指标和猪β防御素基因表达的影响[J]. 动物营养学报, 2020 , 32(7) : 3089 -3098 . DOI: 10.3969/j.issn.1006-267x.2020.07.018

Abstract

This experiment was conducted to investigate the effects of effects of lactoferrin(LF) on reproductive performance of Dahe black sow and iron nutrition, blood cell parameters, immune indexes and porcine β-defensin (pBD) gene expression of sows and piglets. Sixty multiparity (3 to 4 fetuses) Dahe black sows with similar litter size and body weight were randomly divided into 5 groups with 12 replicates in each group and 1 sow in each replicate. From the 80th day of gestation to the delivery, sows the control group were fed a basal diet, sows in the LF1, LF2 and LF3 groups were fed the basal diets supplemented with 100, 200 and 300 mg/kg LF, respectively, and sows in the iron glycine chelate (Fe-Gly) were fed the basal diet supplemented with 500 mg/kg Fe-Gly. The reproductive performance of sows was examined, the red blood cell count (RBC) and hemoglobin (HGB) content in blood, serum immune indexes, sow serum and milk iron content were measured, and the expression level of pBD gene in liver, spleen and duodenal of piglets were detected. The results showed as follows:1) compared with the control group, the weight at birth and litter weight at birth of piglets of LF2 group were significantly increased (P<0.05), the litter weight at birth of piglets of LF1 and LF3 groups was significantly increased (P<0.05). 2) Compared with the control group, the blood RBC and HGB content of sows of LF2, LF3 and Fe-Gly groups were significantly increased (P<0.05), and the blood RBC and HGB content of piglets of LF1, LF2, LF3 and Fe-Gly groups were significantly increased (P<0.05). 3) Compared with the control group, the contents of iron in serum and milk of sows of LF1, LF2 and LF3 groups were significantly increased (P<0.05). 4) Compared with the control group, the serum lactate dehydrogenase (LDH) activity of piglets of LF1, LF2 and LF3 groups and Fe-Gly groups was significantly decreased (P<0.05), and the serum nitric oxide synthase (NOS) activity was significantly increased (P<0.05); the serum immunoglobulin G (IgG) content and lysozyme (LSZ) activity of piglets of LF1 and LF2 groups were significantly increased (P<0.05). 5) Compared with the control group, the expression levels of pBD-1 gene in liver, spleen and duodenum, pBD-2 gene in liver and spleen and pBD-3 gene in spleen of piglets of LF2 group were significantly increased (P<0.05); the expression levels of pBD-1 and pBD-2 genes in liver of piglets of LF1 group and pBD-1 in liver and spleen of piglets of LF3 group were significantly increased (P<0.05); and the expression levels of pBD-2 gene in duodenum and pBD-3 gene in liver and duodenum of piglets of LF1, LF2, LF3 and Fe-Gly groups were significantly decreased (P<0.05). In conclusion, dietary adding appropriate dose (200 mg/kg) LF of Dahe black sow in the late pregnancy can increase the weight at birth and litter weight at birth of piglets, improve the iron nutrition and serum immune indexes of piglets, increase the expression levels of pBD-1 gene in liver, spleen and duodenum, which is beneficial to improve the disease resistance of piglets.

Key words： lactoferrin; Dahe black sows; reproductive performance; hemoglobin; porcine β-defensin

参考文献

[1] 李明丽,罗会琴,鲁绍雄,等.从种猪繁育角度提高母猪年生产力[J].家畜生态学报,2019,40(2):46-49,55.
[2] SKAAR E P.The battle for iron between bacterial pathogens and their vertebrate hosts[J].PLoS Pathogens,2010,6(8):e1000949.
[3] 卓钊.不同铁源对机体铁代谢的影响及其在肠道中的吸收机制研究[D].博士学位论文.杭州:浙江大学,2017:14-17.
[4] 陈婉如,曾丽莉,郭庆,等.氨基酸Fe络合物对妊娠母猪Fe营养状况、繁殖性能及新生仔猪Fe营养状况的影响[J].福建农林大学学报(自然科学版),2003,32(2):230-233.
[5] DRAGO-SERRANNO M E,CAMPOS-RODRIGUEZ R,CARRERO J C,et al.Lactoferrin and peptide-derivatives:antimicrobial agents with potential use in nonspecific immunity modulation[J].Current Pharmaceutical Design,2018,24(10):1067-1078.  
[6] 梁钰,张坤,王梦云,等.猪β防御素研究进展[J].中国畜牧兽医,2019(8):2346-2353.
[7] 安清聪,徐娜娜,张春勇,等.不同水平乳铁蛋白对滇撒配套系仔猪生产性能、小肠形态学和机体抗病能力的影响[J].畜牧兽医学报,2015,46(12):2206-2217.
[8] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408.  
[9] 李尚,曹洪战,芦春莲.妊娠母猪日粮添加铁对仔猪的影响[J].猪业科学,2018,35(1):137-139.
[10] WAN D,ZHANG Y M,WU X,et al.Maternal dietary supplementation with ferrous N-carbamylglycinate chelate affects sowreproductive performance and iron status of neonatal piglets[J].Animal,2018,12(7):1372-1379.  
[11] BUFFLER M,BECKER C,WINDISCH W M.Effects of different iron supply to pregnant sows (Sus scrofa domestica L.) on reproductive performance as well as iron status of new-born piglets[J].Archives of Animal Nutrition,2017,71(3):219-230.  
[12] 董冬华.不同铁源及添加水平对母猪和仔猪铁营养状况影响的研究[D].硕士学位论文.泰安:山东农业大学,2014:20-49.
[13] 袁明贵,高彪,陈文露,等.甘氨酸铁络合物对杜长大仔猪血常规指标的影响[J].广东畜牧兽医科技,2017,42(2):41-44.
[14] LI Y,YANG W R,DONG D H,et al.Effect of different sources and levels of iron in the diet of sows on iron status in neonatal pigs[J].Animal Nutrion,2018,4(2):197-202.  
[15] 李妍.不同铁源及添加水平对断奶仔猪铁营养影响的研究[D].硕士学位论文.泰安:山东农业大学,2016:19-20.
[16] HU P,ZHAO D Y,ZHAO F Z,et al.The effects of the combination of oral lactoferrin and iron injection on iron homestasis,antioxidative abilities and cytokines activities of suckling piglets[J].Animals,2019,9(7):438.
[17] HENTZE M W,MUCKENTHALER M U,GALY B,et al.Two to tango:regulation of mammalian iron metabolism[J].Cell,2010,142(1):24-38.  
[18] 李美荃.血红素铁对妊娠母猪和新生仔猪生产成绩、血红蛋白及Hepcidin铁调作用的BMP-SMAD信号通路研究[D].博士学位论文.昆明:云南农业大学,2017:5-27.
[19] 刘杰.免疫增强物质对母仔猪的健康调控研究[D].硕士学位论文.南京:南京农业大学,2011:23-42.
[20] 吴飞.妊娠后期饲粮添加共轭亚油酸影响母猪初乳IgG的作用机理[D].博士学位论文.北京:中国农业大学,2017:13-20.
[21] 陈玲.妊娠后期营养水平对初产母猪繁殖性能及免疫机能的影响[D].硕士学位论文.大庆:黑龙江八一农垦大学,2018:18-40.
[22] 徐奇友,单安山,李建平.乳铁蛋白对早期断奶仔猪血清生化指标影响的研究[J].中国畜牧杂志,2006,42(3):34-36.
[23] 皮冰冰,赵晓,吕萍萍,等.不同来源乳铁蛋白及乳铁蛋白素对小鼠脾淋巴细胞增殖影响的比较[J].食品科学,2018,39(13):184-189.
[24] SHAN T,WANG Y,WANG Y,et al.Effect of dietary lactoferrin on the immune functions and serum iron level of weanling piglets[J].Journal of Animal Science,2007,85(9):2140-2146.  
[25] JANG Y S,SEO G Y,LEE J M,et al.Lactoferrin causes IgA and IgG2b isotype switching through betaglycan binding and activation of canonical TGF-β signaling[J].Mucosal Immunology,2015,8(4):906-917.  
[26] TAKEUCHI T,HAYASHIDA K I,INAGAKI H,et al.Opioid mediated suppressive effect of milk-derived lactoferrin on distress induced by maternal separation in rat pups[J].Brain Research,2003,979(1/2):216-224.
[27] 方升林.过量铁对机体损伤效应及其诱导细胞死亡的机制研究[D].博士学位论文.杭州:浙江大学,2018:6-10.
[28] 周联,俞瑜.防御素与先天性免疫及获得性免疫[J].国际免疫学杂志,2005,28(2):68-72.
[29] 杨东亮,刘嘉,吴珺,等.肝脏免疫学研究若干进展与挑战[J].实用肝脏病杂志,2019,22(5):609-612.
[30] 黄佳铭,杨冬雪,李晓曦,等.肠道黏膜免疫与炎症小体的研究进展[J].微生物与感染,2019,14(2):113-123.
[31] 苏丹萍,蒋爱翔,贺东生.猪的免疫器官及其生理作用[J].猪业科学,2010,27(10):26-29.
[32] WANG J,HUANG N N,XIONG J,et al.Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase inhibition[J].International immunopharmacology,2018,65:303-311.
[33] ZENG X F,SUNKARA L R,JIANG W Y,et al.Induction of porcine host defense peptide gene expression by short-chain fatty acids and their analogs[J].PLoS One,2013,8(8):e72922.
[34] KUSUMANINGSIH T,SUBIJANTO M S,INDRAWATI R,et al.The level of beta defensin-2 in saliva and its expression in parotid gland epithelial cells after probiotic (Lactobacillus reuteri) induction to inhibit Streptococcus mutansin caries[J].European Journal of Dentistry,2016,10(4):556-560.  
[35] KOBAYASHI R,KOBAYASHI T,SAKAI F et al.Oral administration of Lactobacillus gasseri SBT2055 is effective in preventing Porphyromonas gingivalis-accelerated periodontal disease[J].Scientific Reports,2014,7:545.
[36] 刘璨颖,陈志胜,林裕锋,等.猪源β-防御素2和3的研究进展[J].中国畜牧杂志,2019,55(8):30-34.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2