禽营养与饲料 POULTRY NUTRITION AND FEED

胚蛋给养N-乙酰-L-谷氨酸对拉萨白鸡种蛋孵化性能和鸡胚肠道形态的影响

  • 王继光 ,
  • 宋志刚 ,
  • 武书庚 ,
  • 王晶 ,
  • 齐广海 ,
  • 张海军 ,
  • 冯静 ,
  • 马雪英
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  • 1. 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 生物饲料开发国家工程研究中心, 北京 100081;
    2. 山东农业大学动物科技学院, 泰安 271018;
    3. 西藏自治区农牧科学院畜牧兽医研究所, 拉萨 850000
王继光(1993-),男,山东临沂人,硕士研究生,从事动物营养与饲料科学研究。E-mail:wangjiguang20@163.com

收稿日期: 2020-01-10

  网络出版日期: 2020-07-15

基金资助

现代农业产业技术体系建设专项资金(CARS-40-K12);北京市自然科学基金(6202029);国家重点研发计划(2018YFD0500403)

Effects of in Ovo Feeding of N-Ncetyl-L-Glutamate on Hatching Performance of Hatching Eggs and Intestinal Morphology of Chicken Embryos for Lhasa White Chickens

  • WANG Jiguang ,
  • SONG Zhigang ,
  • WU Shugeng ,
  • WANG Jing ,
  • QI Guanghai ,
  • ZHANG Haijun ,
  • FENG Jing ,
  • MA Xueying
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  • 1. Key Laboratory of Feed Biotechnology of Ministry of Agriculture&Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
    3. Institute of Animal Husbandry and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850000, China

Received date: 2020-01-10

  Online published: 2020-07-15

摘要

本试验旨在研究胚蛋给养N-乙酰-L-谷氨酸(NAG)对拉萨白鸡种蛋孵化性能以及鸡胚生长、肠道形态的影响。选取17.0胚龄拉萨白鸡活胚蛋240枚,随机分为对照组和NAG给养组,每个组6个重复,每个重复20枚活胚蛋。17.5胚龄时,NAG给养组经羊膜腔无菌注射1.5% NAG营养液,注射剂量为0.1 mL,即NAG补给量为1.5 mg/枚;对照组种蛋不做处理。在19.5胚龄观测鸡胚生长指标、肠道形态,出壳当天统计孵化性能。结果表明:1)孵化后期胚蛋给养NAG对拉萨白鸡鸡胚生长无显著影响(P>0.05)。2)NAG给养组种蛋孵化率显著高于对照组(P<0.05),健雏率2组间无显著差异(P>0.05)。3)与对照组相比,胚蛋给养NAG显著降低了19.5胚龄鸡胚十二指肠火箭形绒毛比例(P<0.05),对空肠和回肠各类型绒毛比例无显著影响(P>0.05)。4)与对照组相比,胚蛋给养NAG显著提高了19.5胚龄鸡胚回肠手指形绒毛高度(P<0.05),对回肠手指形绒毛宽度无显著影响(P>0.05);NAG给养组19.5胚龄鸡胚十二指肠、空肠手指形绒毛高度和宽度较对照组无显著差异(P>0.05)。由此可见,孵化后期(17.5胚龄)经由羊膜腔给养1.5 mg/枚NAG可提高拉萨白鸡种蛋的孵化率,对鸡胚生长未产生显著影响,但可促进鸡胚肠道发育。

本文引用格式

王继光 , 宋志刚 , 武书庚 , 王晶 , 齐广海 , 张海军 , 冯静 , 马雪英 . 胚蛋给养N-乙酰-L-谷氨酸对拉萨白鸡种蛋孵化性能和鸡胚肠道形态的影响[J]. 动物营养学报, 2020 , 32(7) : 3135 -3142 . DOI: 10.3969/j.issn.1006-267x.2020.07.023

Abstract

The current study was conducted to investigate the effects of in ovo feeding (IOF) of N-acetyl-L-glutamate (NAG) on hatching performance of hatching eggs, growth and intestinal morphology of chicken embryos for Lhasa white chickens. A total of 240 Lhasa white chicken embryo eggs aged 17 d of embryogenesis were randomly allotted to control group and NAG-injection group. Each group contained 6 replicates with 20 embryo eggs each. Embryo eggs in the control group were not injected. Embryo eggs in the NAG-injection group were injected with 0.1 mL of 1.5% NAG nutrient solution at 17.5 d of embryogenesis, that was NAG supplementary amount was 1.5 mg/egg. The growth and intestinal morphology of chicken embryos at 19.5 d of embryogenesis were determined, and the hatching performance was calculated when eggs hatched out. The results showed as follows:1) IOF of NAG did not affect the growth of chicken embryos (P>0.05). 2) The hatchability of hatching eggs of NAG-injected group was significantly higher than that of control group (P<0.05), but the rate of healthy chicks had no significant difference between NAG-injected group and control group (P>0.05). 3) Compared with the control group, IOF of NAG significantly decreased the proportion of rocket-shaped villi in duodenum of chicken embryos at 19.5 d of embryogenesis (P<0.05), but had no significant effects on the proportions of different types of villus in jejunum and ileum (P>0.05). 4) Compared with the control group, IOF of NAG significantly increased the height but not the width of finger-shaped villi in ileum of chicken embryos at 19.5 d of embryogenesis (P<0.05), and had no significant effects on the height and width of finger-shaped villi in duodenum and jejunum (P>0.05). It is concluded that IOF of NAG (1.5 mg/egg) via amniotic injection at the late stage of incubation (17.5 d of embryogenesis) can increase the hatchability of hatching eggs for Lhasa white chickens, and promote the intestinal development without affecting the growth of chicken embryos.

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