反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

N-氨甲酰谷氨酸对荷斯坦奶公牛氮代谢的影响

  • 孙雪丽 ,
  • 李秋凤 ,
  • 曹玉凤 ,
  • 李树静 ,
  • 张秀江 ,
  • 沈宜钊 ,
  • 李建国 ,
  • 高艳霞 ,
  • 王美美
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  • 1. 河北农业大学动物科技学院, 保定 071001;
    2. 河北天和肉牛养殖有限公司, 石家庄 050200;
    3. 保定市畜牧工作站, 保定 071051
孙雪丽(1992-),女,河北保定人,硕士研究生,从事反刍动物营养与饲料研究。E-mail:1277512374@qq.com

收稿日期: 2020-02-03

  网络出版日期: 2020-07-15

基金资助

国家现代农业产业技术体系(CARS-37);河北省二期现代农业产业技术体系肉牛创新团队高效养殖岗位建设项目(HBCT2018130202)

Effects of N-Carbamylglutamate on Nitrogen Metabolism of Holstein Bulls

  • SUN Xueli ,
  • LI Qiufeng ,
  • CAO Yufeng ,
  • LI Shujing ,
  • ZHANG Xiujiang ,
  • SHEN Yizhao ,
  • LI Jianguo ,
  • GAO Yanxia ,
  • WANG Meimei
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  • 1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China;
    2. Hebei Tianhe Beef Cattle Breeding Co., Ltd., Shijiazhuang 050200, China;
    3. Animal Husbandry Workstation of Baoding, Baoding 071051, China

Received date: 2020-02-03

  Online published: 2020-07-15

摘要

本试验旨在研究饲粮中添加不同水平N-氨甲酰谷氨酸(NCG)对荷斯坦奶公牛氮代谢的影响。选择45头健康的荷斯坦奶公牛[初始体重为(478.00±13.66)kg,12~13月龄],随机分成3组,每组15头,分别添加0(对照组)、15、25 g/d的NCG。预试期7 d,正试期90 d。结果表明:1)与对照组相比,饲粮中添加15与25 g/d的NCG显著提高血浆中精氨酸的含量(P<0.05),分别提高了14.05%、8.30%。2)与对照组相比,饲粮中添加15 g/d的NCG显著降低血清中尿素氮含量(P<0.05)。3)饲粮中添加NCG对可消化有机物质的采食量以及尿中尿囊素、尿酸、嘌呤衍生物排出量无显著影响(P>0.05)。4)与对照组相比,饲粮中添加15 g/d的NCG显著提高奶公牛氮沉积、净蛋白质利用率与蛋白质生物学价值(P<0.05)。由此可见,在本试验条件下,饲粮中添加15 g/d NCG时,荷斯坦奶公牛氮沉积、净蛋白质利用率和蛋白质的生物学价值较为理想。

本文引用格式

孙雪丽 , 李秋凤 , 曹玉凤 , 李树静 , 张秀江 , 沈宜钊 , 李建国 , 高艳霞 , 王美美 . N-氨甲酰谷氨酸对荷斯坦奶公牛氮代谢的影响[J]. 动物营养学报, 2020 , 32(7) : 3224 -3233 . DOI: 10.3969/j.issn.1006-267x.2020.07.032

Abstract

This experiment was conducted to investigate the effects of dietary supplementation of N-carbamylglutamate (NCG) in different levels on nitrogen metabolism of Holstein bulls. Forty-five healthy Holstein bulls[initial body weight of (478.00±13.66) kg, 12 to 13 months of age] were randomly assigned into three groups with 15 replicates in each group, and were fed diets supplemented with 0 (control), 15 and 25 g/d of NCG, respectively. Adaptation period was 7 days and test period was 90 days. The results showed as follows:1) compared with control group, adding 15, 25 g/d NCG significantly increased the plasma content of arginine by 14.05% and 8.30% (P<0.05). 2) Adding 15 g/d NCG significantly decreased the content of serum urea nitrogen compared with control group (P<0.05). 3) Adding NCG had no significant effect on digestible organic matter intake, the contents of allantoin, uric acid and purine derivatives excreted in urine (P>0.05). 4) Compared with control group, adding 15 g/d NCG significantly increased nitrogen retention, nitrogen protein utilization and protein biological value (P<0.05). It is concluded that under the conditions of this experiment, when the NCG supplemental level is 15 g/d, the nitrogen retention, nitrogen protein utilization and protein biological value of the Holstein bulls are optimal.

参考文献

[1] NOFTSGER S,ST-PIERRE N R.Supplementation of methionine and selection of highly digestible rumen undegradable protein to improve nitrogen efficiency for milk production[J].Journal of Dairy Science,2003,86(3):958-969.  
[2] WANG C, LIU H Y, WANG Y M, et al.Effects of dietary supplementation of methionine and lysine on milk production and nitrogen utilization in dairy cows[J].Journal of Dairy Science,2010,93(8):3661-3670.  
[3] YIN J D,PIAO X S,PIAO X S,et al.Regulation of insulin-like growth factor-1 and growth hormone receptor gene expression in weaned pigs fed graded levels of dietary tryptophan[J].Journal of Animal & Veterinary Advances,2004,3(8):490-496.
[4] 王星凌,陶海英,游伟,等.日粮蛋白质和赖氨酸水平对奶牛产奶性能、氮代谢和血液指标的影响[J].中国奶牛,2015(13):10-14.
[5] EFRON D,BARBUL A.Role of arginine in immunonutrition[J].Journal of Gastroenterology,2000,35 Suppl 12:20-23.
[6] MEIJER A J,LOF C,RAMOS I C,et al.Control of ureogenesis[J].European Journal of Biochemistry,1985,148(1):189-196.  
[7] 万春孟.水貂育成期及冬毛期饲粮适宜精氨酸水平研究[D].硕士学位论文.北京:中国农业科学院,2016.
[8] 杨荣,王华朗,宋增廷,等.精氨酸在畜禽营养生理中的应用进展[J].广东饲料,2019,28(7):36-39.
[9] DAVENPORT G M,BOLING J A,SCHILLO K K.Nitrogen metabolism and somatotropin secretion in beef heifers receiving abomasal arginine infusions[J].Journal of Animal Science,1990,68(6):1683-1692.  
[10] CHACHER B,WANG D M,LIU H Y,et al.Degradation of L-arginine and N-carbamoyl glutamate and their effect on rumen fermentation in vitro[J].Italian Journal of Animal Science,2012,11(4):e68.
[11] 李新花,赖玉娇,王春平.N-氨甲酰谷氨酸在反刍动物生产中应用研究进展[J].中国奶牛,2016(2):8-11.
[12] 刘星达,彭瑛,吴信,等.精氨酸和精氨酸生素对母猪泌乳性能及哺乳仔猪生长性能的影响[J].饲料工业,2011,32(8):14-16.
[13] CHACHER B.N-甲酰谷氨酸作为精氨酸增强剂的评定及其对高产奶牛瘤胃发酵、泌乳性能和氮利用的影响[D].博士学位论文.杭州:浙江大学,2013.
[14] 中华人民共和国农业部.NY/T815-2004肉牛饲养标准[S].北京:中国农业出版社,2004.
[15] 中华人民共和国国家质量监督检验检疫总局.GB/T 6432-1994饲料中粗蛋白测定方法[S].北京:中国标准出版社,1994.
[16] 张丽英.饲料分析及饲料质量检测技术[M].第3版.北京:中国农业大学出版社,2007.
[17] GRAY N,ZIA R,KING A,et al.High-speed quantitative UPLC-MS analysis of multiple amines in human plasma and serum via precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate:application to acetaminophen-induced liver failure[J].Journal of Proteome Research,2017,89(4):2478-2487.
[18] 王建华,王洪荣,张海鹰,等.瘤胃保护性氨基酸对内蒙古白绒山羊氮消化代谢的影响[J].畜牧兽医学报,2006,37(1):38-43.
[19] 钱鑫,李雪艳,姜淑贞,等.氨基酸水平对莱芜黑仔猪生产性能和血清生化指标及游离氨基酸浓度的影响[J].中国畜牧杂志,2016,52(13):55-59.
[20] WU G Y,BAZER F W,DAVIS T A,et al.Arginine metabolism and nutrition in growth,health and disease[J].Amino Acids,2009,37(1):153-168.  
[21] MEIJER A J,DUBBELHUIS P F.Amino acid signalling and the integration of metabolism[J].Biochemical and Biophysical Research Communications,2004,313(2):397-403.  
[22] FRANK J W,ESCOBAR J,NGUYEN H V,et al.Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets[J].The Journal of Nutrition,2007,137(2):315-319.  
[23] WU G Y,JAEGER L A,BAZER F W,et al.Arginine deficiency in preterm infants:biochemical mechanisms and nutritional implications[J].Journal of Nutritional Biochemistry,2004,15(8):442-451.  
[24] 徐运杰.母仔猪精氨酸的生理代谢与营养作用[J].猪业科学,2020,37(1):84-89.
[25] 查伟.饲粮添加脯氨酸对环江香猪繁殖性能及其后代生长发育的影响[D].硕士学位论文.南昌:江西农业大学,2015.
[26] 王琤.精氨酸对仔猪宫内发育及N-氨甲酰谷氨酸对断奶仔猪生长的影响[D].博士学位论文.南昌:江西农业大学,2012.
[27] 王天辉.L-精氨酸和支链氨基酸对大鼠心肌相对缺血/再灌注损伤协同保护作用的研究[D].硕士学位论文.北京:中国人民解放军军事医学科学院,2005.
[28] 李金茂,赖大年.复方支链氨基酸注射液对贲门癌根治术后患者蛋白质代谢的影响[J].中华胃肠外科杂志,2005,8(2):137-140.
[29] HAN J M,JEONG S J,PARK M C,et al.Leucyl-tRNA synthetase is an intracellular leucine sensor for the mTORC1-signaling pathway[J].Cell,2012,149(2):410-424.  
[30] 李颖,项爱丽,郑百芹,等.氨基酸对奶牛乳腺蛋白质合成调控及其分子机制的研究进展[J].中国畜牧兽医,2016,43(7):1774-1779.
[31] 周笑犁,汤文杰,孔祥峰,等.精氨酸对环江香猪营养物质消化率和血液指标的影响[J].华北农学报,2012,27(4):234-238.
[32] 汪益峰,周维仁,章世元,等.氨基酸平衡和外源酶对异育银鲫的生长、氮代谢及血液生化指标的影响[J]. 江苏农业学报, 2010(1):136-141.
[33] 邓新为,裘博文,郝柳柳,等.日粮添加发酵巨菌草对香猪生长期血液生化指标的影响[J].饲料工业,2020,41(6):14-17.
[34] 宾石玉,印遇龙,黄瑞林,等.日粮淀粉来源对断奶仔猪内脏器官蛋白质合成率的影响[J].核农学报,2006,20(6):535-538.
[35] MCALLAN A B,SMITH R H.Degradation of nucleic acid derivatives by rumen bacteria in vitro[J].British Journal of Nutrition,1973,29(3):467-474.  
[36] MCALLAN A B.The fate of nucleic acids in ruminants[J].Proceedings of the Nutrition Society,1982,41(3):309-316.  
[37] 张艳梅,师周戈,焦光月.拉沙里菌素对西门塔尔牛瘤胃发酵和尿中嘌呤衍生物含量的影响[J].中国奶牛,2015(23):7-10.
[38] BALCELLS J,GUADA J A,CASTRILLO C,et al.Urinary excretion of allantoin and allantoin precursors by sheep after different rates of purine infusion into the duodenum[J].The Journal of Agricultural Science,1991,116(2):309-317.  
[39] 钟伟,龙瑞军,LIANG J B,等.不同比例香根草日粮对沼泽性水牛尿嘌呤衍生物排出量的影响[J].甘肃农业大学学报,2007,42(1):25-29.
[40] CHEN X B,HOVELL F D,ØRSKOV E R,et al.Excretion of purine derivatives by ruminants:effect of exogenous nucleic acid supply on purine derivative excretion by sheep[J].British Journal of Nutrition,1990,63(1):131-142.  
[41] 阳伏林,王虎成,郭旭生,等.用尿中嘌呤衍生物估测瘤胃微生物蛋白产量的研究进展[J].草业学报,2008,17(1):121-129.
[42] 王永新,莫放,董宽虎,等.肉牛混合日粮不同进食水平对尿中嘌呤衍生物排出量的影响[J].中国草食动物,2008,24(12):11-15.
[43] 黄鸿威,莫放,周汉林.不同营养水平对4-6月龄荷斯坦犊牛尿中嘌呤衍生物排出量的影响[J].中国畜牧杂志,2005,41(6):15-18.
[44] 李丽莉,梁坤,韦升菊,等.不同日粮采食水平对青年母水牛尿中嘌呤衍生物排出规律的影响研究[J].黑龙江畜牧兽医,2009(23):11-14.
[45] 刘浩,周建伟,张瑛,等.燕麦干草对藏羊尿中嘌呤衍生物、肌酐及马尿酸排出量的影响[J].家畜生态学报,2014,35(9):38-44.
[46] VAN HORN H H,NEWTON G L,KUNKLE W E.Ruminant nutrition from an environmental perspective:factors affecting whole-farm nutrient balance[J].Journal of Animal Science,1996,74(12):3082-3102.  
[47] BARBUL A.Arginine:biochemistry,physiology,and therapeutic implications[J].Journal of Parenteral and Enteral Nutrition,1986,10(2):227-238.  
[48] NICHOLSON B,MANNER C K,KLEEMAN J,et al.Sustained nitric oxide production in macrophages requires the arginine transporter CAT2[J].Journal of Biological Chemistry,2001,276(19):15881-15885.  
[49] 王琤,王仁华.N-氨甲酰谷氨酸在仔猪生产中的研究应用进展[J].粮食与饲料工业,2015(9):49-50,58.
[50] 方桂友,周万胜,邱华玲,等.夏季高温时蛋白质和赖氨酸水平对泌乳母猪生产性能及粪氮排泄量的影响[J].福建畜牧兽医,2018,40(2):3-7.
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