水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

添加牛磺酸和硒酵母对改善棉籽浓缩蛋白替代条纹锯饲料鱼粉的作用

  • 张静雅 ,
  • 任幸 ,
  • 李伟业 ,
  • 王力 ,
  • 王岩
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  • 1. 浙江大学海洋学院, 舟山 316021;
    2. 舟山市水产研究所, 舟山 316000
张静雅(1995-),女,浙江绍兴人,硕士研究生,研究方向为鱼类营养。E-mail:2417782858@qq.com

收稿日期: 2020-01-20

  网络出版日期: 2020-07-15

基金资助

国家自然科学基金(31772868)

Influence of Taurine and Selenium Yeast Supplementation on Replacing Fish Meal with Cottonseed Protein Concentrate in Black Sea Bass (Centropristis striata) Diet

  • ZHANG Jingya ,
  • REN Xing ,
  • LI Weiye ,
  • WANG Li ,
  • WANG Yan
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  • 1. Ocean College, Zhejiang University, Zhoushan 316021, China;
    2. Zhoushan Fisheries Research Institute, Zhoushan 316000, China

Received date: 2020-01-20

  Online published: 2020-07-15

摘要

本试验旨在研究添加牛磺酸和硒酵母对改善棉籽浓缩蛋白替代条纹锯(Centropristis striata)饲料鱼粉的作用。采用双因素试验设计,设2个饲料鱼粉替代水平和2个牛磺酸与硒酵母混合物的添加水平。对照(PC)饲料鱼粉水平为35%,利用棉籽浓缩蛋白分别替代PC饲料中鱼粉的60%(H)和80%(L);在相同鱼粉替代水平下,分别添加(ST)或不添加(N)牛磺酸与硒酵母的混合物。5种试验饲料分别为PC、HST、LST、HN和LN饲料。选择初始体重为(29.5±0.5)g的条纹锯360尾,分为5组(每组3个重复,每个重复24尾鱼),分别投喂5种试验饲料。试验期10周。结果表明:摄食不同饲料对鱼摄食率、生长、饲料利用效率、鱼体组成(粗灰分和磷含量除外)以及养殖废物排放量均未产生显著影响(P>0.05),但显著影响成活率(P<0.05)。摄食HST和LST饲料的鱼成活率显著高于摄食PC和LN饲料的鱼(P<0.05)。摄食PC、HST和LST饲料的鱼粗灰分和磷含量显著低于摄食HN和LN饲料的鱼(P<0.05)。摄食PC饲料与摄食LST饲料时养殖效益接近,与摄食HST、HN和LN饲料时的养殖效益存在明显差异。摄食PC饲料的鱼血浆谷胱甘肽过氧化物酶(GPx)活性显著高于摄食LN饲料的鱼(P<0.05),摄食PC和LST饲料的鱼肝脏丙二醛(MDA)含量显著低于摄食LN饲料的鱼(P<0.05)。由此可见,添加牛磺酸和硒酵母未明显提高利用棉籽浓缩蛋白替代条纹锯饲料中鱼粉的水平,但在一定程度上有益于改善摄食低鱼粉水平饲料时鱼的成活率和养殖效益。

本文引用格式

张静雅 , 任幸 , 李伟业 , 王力 , 王岩 . 添加牛磺酸和硒酵母对改善棉籽浓缩蛋白替代条纹锯饲料鱼粉的作用[J]. 动物营养学报, 2020 , 32(7) : 3291 -3302 . DOI: 10.3969/j.issn.1006-267x.2020.07.039

Abstract

This trial was conducted to evaluate the influence of taurine and selenium yeast supplementation on replacing fish meal with cottonseed protein concentrate (CPC) in black sea bass (Centropristis striata) diet. A two factor trial design was adopted, including two dietary fish meal replacement levels and two taurine and selenium yeast supplementation levels. The control (PC) diet contained 35% fish meal, and CPC was used as a fish meal substitute to replace 60% (H) and 80% (L) of the fish meal in PC diet. At the same fish meal replacement level, a taurine and selenium yeast mix was added (ST) or not added (N). The five test diets were abbreviated as PC, HST, LST, HN, LN. A total of 360 Centropristis striata with initial body weight of (29.5±0.5) g were divided into 5 groups (each group contained 3 replicates and each replicate contained 24 fish) which were fed with the test diets. The feeding trial lasted for 10 weeks. The results showed that no significant differences were found in feed intake, growth, feed conversion ratio, body composition (except ash and phosphorus contents) and waste outputs among fish fed with different diets (P>0.05). The survival rate of fish fed HST and LST diets was significantly higher than that of fish fed PC and LN diets (P<0.05). The ash and phosphorus contents of fish fed PC, HST and LST diets were significantly lower than those of fish fed HN and LN diets (P<0.05). The farming benefit of the fish fed PC diet was close to that of fish fed LST diet, but was obviously different from that of fish fed HST, HN and LN diets. The plasma glutathione peroxidase (GPx) activity of fish fed PC diet was significantly higher than that of fish fed LN diet (P<0.05), and the liver malondialdehyde (MDA) content of fish fed PC and LST diets was significantly lower than that of fish fed LN diet (P<0.05). In conclusion, the taurine and selenium yeast supplementation cannot significantly elevate fish meal replacement level by CPC in Centropristis striata diet, but to a certain extent, can improve the survival rate and farming benefits of fish fed low fish meal diets.

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