实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

添加菌酶复合制剂发酵玉米秸秆的筛选试验

  • 吴立坡 ,
  • 姜宁 ,
  • 张爱忠
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  • 1. 黑龙江八一农垦大学动物科技学院, 黑龙江省寒区饲料资源高效利用与营养调控重点实验室, 大庆 163319;
    2. 畜禽养殖污染控制与资源化技术国家工程重点实验室, 长沙 410125
吴立坡(1994-),男,河北石家庄人,硕士研究生,从事动物营养与饲料科学研究。E-mail:321897719@qq.com

收稿日期: 2020-01-31

  网络出版日期: 2020-07-15

基金资助

国家重点研发计划项目(2018YFD0502100);黑龙江八一农垦大学平台支持计划(PTJH201903);黑龙江八一农垦大学研究生创新科研项目(YJSCX2019-Y20)

Screening Experiment of Fermented Corn Stalk with Fungi and Enzyme Mixed Preparation

  • WU Lipo ,
  • JIANG Ning ,
  • ZHANG Aizhong
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  • 1. Key Laboratory of High Efficiency Utilization and Nutrition Manipulation of Feed Resources in Cold Areas of Heilongjiang Province, College of Animal Science and Technology, Heilongjiang Bayi Agricultural Reclamation University, Daqing 163319, China;
    2. National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Changsha 410125, China

Received date: 2020-01-31

  Online published: 2020-07-15

摘要

本试验旨在探究菌酶复合制剂发酵玉米秸秆的最佳发酵条件及发酵效果。运用均匀设计试验,以中性洗涤纤维降解率、酸性洗涤纤维降解率为目的指标,对黄孢原毛平革菌(Phanerochaete chrysosporium)接种量、纤维素酶和木聚糖酶添加量及发酵天数4个因素进行筛选,并对发酵效果进行评价和比较分析。结果表明:菌酶复合制剂发酵玉米秸秆的干物质、粗蛋白质含量显著高于未发酵秸秆(P<0.05),酸性洗涤木质素、中性洗涤纤维、酸性洗涤纤维含量显著低于未发酵秸秆(P<0.05)。本研究所获得的最佳发酵条件为:纤维素酶添加量0.2 g/kg,木聚糖酶添加量0.1 g/kg,黄孢原毛平革菌接种量20%,发酵天数19 d;在此条件下,玉米秸秆的中性洗涤纤维降解率和酸性洗涤纤维降解率分别达到了23.47%和20.13%。

本文引用格式

吴立坡 , 姜宁 , 张爱忠 . 添加菌酶复合制剂发酵玉米秸秆的筛选试验[J]. 动物营养学报, 2020 , 32(7) : 3358 -3364 . DOI: 10.3969/j.issn.1006-267x.2020.07.045

Abstract

The purpose of this experiment was to explore the optimum fermentation condition and effects of corn stalk fermented with fungi and enzyme mixed preparation. The degradable rate of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were used as the evaluative indices in the uniform design, the fermentation days and the supplemented proportion of Phanerochaete chrysosporium, cellulase, xylanase were evaluated and screened. The results showed that the contents of dry matter, crude protein in fermented stalk were significantly higher than those in the unfermented stalk (P<0.05) and the contents of acid detergent lignine, NDF and ADF in fermented stalk were significantly lower than those in unfermented stalk (P<0.05). The optimum ferment condition:cellulase is 0.2 g/kg, xylanase is 0.1 g/kg, Phanerochaete chrysosporium is 20% and fermentation day is 19 days. The degradable rates of NDF and ADF are 23.47% and 20.13%, respectively.

参考文献

[1] 张克威,赵和平,薛艳林,等.乳酸菌和纤维素酶对玉米秸秆黄贮饲料品质的影响[J].畜牧与饲料科学,2017,38(4):20-24.
[2] 马广英,张文举,徐清华,等.秸秆黄贮优化方案及其对小麦、玉米、油菜秸秆处理的影响[J].中国草食动物科学,2014,34(2):24-27.
[3] 冯冲凌.黄孢原毛平革菌及其关键功能酶对木质纤维素降解转化特性的研究[D].博士学位论文.长沙:湖南大学,2011.
[4] ZHI Z L,WANG H.White-rot fungal pretreatment of wheat straw with Phanerochaete chrysosporium for biohydrogen production:simultaneous saccharification and fermentation[J].Bioprocess and Biosystems Engineering,2014,37(7):1447-1458.  
[5] CHEN Y,WANG Y Y,XU Z,et al.Enhanced humification of maize straw and canola residue during composting by inoculating Phanerochaete chrysosporium in the cooling period[J].Bioresource Technology,2019,293:122075.
[6] VA RSHNEY A K,MONAN M K,VIDYA RTHI A S,et al.Effect of nitrogen sources and C:N ratio on production of manganese peroxidase using Phanero-chaete chrysosporium NCIM 1197[J].Journal of Pure and Applied Microbiology,2014,8(1):481-487.
[7] 李红亚,李文,李术娜,等.解淀粉芽孢杆菌复合菌剂对玉米秸秆的降解作用及表征[J].草业学报,2017,26(6):153-167.
[8] 方开泰.均匀设计及其应用(Ⅳ)[J].数理统计与管理,1994(4):54-56.
[9] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.  
[10] 杨胜.饲料分析及饲料质量检测技术[M].北京:北京农业大学出版社,1993.
[11] 农业部畜牧兽医司.青贮饲料质量评定标准(试行)[J].中国饲料,1996(21):5-7.
[12] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(6/7):37.
[13] CAO Y,TAKAHASHI T,HORIGUCHI K I,et al.Effect of adding lactic acid bacteria and molasses on fermentation quality and in vitro ruminal digestion of total mixed ration silage prepared with whole crop rice[J].Grassland Science,2010,56(1):19-25.  
[14] SONG H T,GAO Y,YANG Y M,et al.Synergistic effect of cellulase and xylanase during hydrolysis of natural lignocellulosic substrates[J].Bioresource Technology,2016,219:710-715.
[15] 毛建红.酶-菌制剂发酵玉米秸秆对其瘤胃降解及微观结构的影响[D].硕士学位论文.阿拉尔:塔里木大学,2018.
[16] 张仲卿,张爱忠,姜宁.混合真菌发酵对玉米秸秆纤维素与木质素降解率的影响[J].动物营养学报,2019,31(3):1385-1395.
[17] GUTIERREZ-CORREA M,PORTAL L,MORENO P,et al.Mixed culture solid substrate fermentation of Trichoderma reesei with Aspergillus niger on sugar cane bagasse[J].Bioresource Technology,1999,68(2):173-178.  
[18] 薛艳林,孙林,殷国梅,等.生物添加剂和填装密度对菊芋青贮饲料品质的影响[J].畜牧与饲料科学,2017,38(1):39-43.
[19] 郭萌萌.复合益生菌发酵全株玉米、玉米秸秆及豆粕的效果研究[D].硕士学位论文.杨凌:西北农林科技大学,2019.
[20] 李茂,字学娟,吕仁龙,等.添加乳酸菌和纤维素酶对王草青贮品质和瘤胃降解率的影响[J/OL].中国畜牧杂志,2020:1-9.[2020-02-06].https://doi.org/10.19556/j.0258-7033.20190813-02.
[21] LI F H,DING Z T,KE W C,et al.Ferulic acid esterase-producing lactic acid bacteria and cellulase pretreatments of corn stalk silage at two different temperatures:ensiling characteristics,carbohydrates composition and enzymatic saccharification[J].Bioresource Technology,2019,282:211-221.
[22] 杨世帆,田鑫淼,张梦瑶,等.添加饲用复合菌剂黄贮玉米秸秆品质的综合评定[J].饲料研究,2019,42(5):82-86.
[23] 贾晶霞,梁宝忠,王艳红,等.不同汽爆预处理对干玉米秸秆青贮效果的影响[J].农业工程学报,2013,29(20):192-198.
[24] 李川东,李建农,沈益新.收获时间对饲用高粱和野生大豆单、混青贮品质的影响[J].中国草地学报,2008,30(5):75-79.
[25] 岳丽,王卉,山其米克,等.复合菌种固态发酵法提高甜高粱秸秆饲料品质的研究[J].饲料工业,2019,40(5):34-39.
[26] MARQUES N P,DE CASSIA PEREIRA J,GOMES E,et al.Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse[J].Industrial Crops and Products,2018,122:66-75.
[27] 张吉鹍,卢德勋.试述反刍动物日粮中的纤维问题[J].中国乳业,2003(7):21-24.
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