动物营养学报 >

2020 , Vol. 32 >Issue 9: 4205 - 4211

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.030

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

布拉迪酵母壁多糖对早期断奶羔羊腹泻及肠道黏膜免疫的影响

  • 刘孟健 ,
  • 张文举 ,
  • 姚峻
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  • 石河子大学动物科技学院, 石河子 832000
刘孟健(1988-),男,新疆伊宁人,博士研究生,研究方向为动物营养与饲料科学。E-mail:liumengjian1988@163.com

收稿日期: 2020-03-14

  网络出版日期: 2020-09-17

基金资助

国家重点研发计划项目（2018YFD0502100）

收起

Effects of Saccharomyces boulardii Cell Wall Polysaccharides on Diarrhea and Intestinal Mucosal Immunity of Early-Weaned Lambs

  • LIU Mengjian ,
  • ZHANG Wenju ,
  • YAO Jun
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  • College of Animal Science and Technology, Shihezi University, Shihezi 832000, China

Received date: 2020-03-14

  Online published: 2020-09-17

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摘要

本研究旨在探讨饲粮中添加布拉迪酵母壁多糖对早期断奶羔羊粪样指数、腹泻频率及肠道黏膜免疫的影响。采用单因素试验设计，选取60只15~17日龄的健康羔羊，按同质原则分为5组，每组3个重复，每个重复4只羔羊。5组试验羔羊分别饲喂代乳料（Ⅰ组）、代乳料+0.005%庆大霉素（Ⅱ组）、代乳料+0.1%布拉迪酵母壁多糖（Ⅲ组）、代乳料+0.3%布拉迪酵母壁多糖（Ⅳ组）、代乳料+0.5%布拉迪酵母壁多糖（Ⅴ组）。试验期共44 d，其中过渡期8 d，预试期7 d，正试期29 d。结果显示：1）Ⅳ组和Ⅴ组的粪样指数和腹泻频率较Ⅰ组分别显著降低了20.73%、15.85%和31.34%、23.75%（P<0.05）。2）Ⅳ组和Ⅴ组结肠黏膜中分泌型免疫球蛋白A（sIgA）含量较Ⅰ组分别显著提高了14.49%和17.77%（P<0.05）；Ⅲ组结肠黏膜中免疫球蛋白A（IgA）含量较Ⅰ组显著提高了5.81%（P<0.05）；Ⅳ组和Ⅴ组结肠黏膜中白细胞介素-1（IL-1）含量较Ⅰ组分别显著降低了7.93%和10.58%（P<0.05）；Ⅳ组结肠黏膜中白细胞介素-10（IL-10）含量较Ⅰ组显著提高8.05%（P<0.05）；此外，Ⅳ组和Ⅴ组结肠黏膜中免疫球蛋白G（IgG）含量有所降低且肿瘤坏死因子-α（TNF-α）、γ-干扰素（IFN-γ）含量有所提高，但与Ⅰ组的差异未达显著水平（P>0.05）。由此可见，代乳料中添加0.3%~0.5%的布拉迪酵母壁多糖能在一定程度上降低早期断奶羔羊的腹泻频率，提高其肠道黏膜免疫水平。

关键词： 布拉迪酵母壁多糖; 腹泻频率; 肠道免疫; 早期断奶羔羊

本文引用格式

刘孟健 , 张文举 , 姚峻 . 布拉迪酵母壁多糖对早期断奶羔羊腹泻及肠道黏膜免疫的影响[J]. 动物营养学报, 2020 , 32(9) : 4205 -4211 . DOI: 10.3969/j.issn.1006-267x.2020.09.030

Abstract

This experiment was conducted to determine the effects of Saccharomyces boulardii cell wall polysaccharides on diarrhea and intestinal mucosal immunity of early-weaned lambs. A single factor random experiment design was used and 60 lambs with similar health condition, parity and body weight which weaned at 15 to 17 days of age were randomly allotted to 5 groups with 3 replicates in each group and 4 lambs in each replicate. Lambs in the 4 groups were fed milk replacer (group Ⅰ), milk replacer+0.005% gentamicin (group Ⅱ), milk replacer+0.1% Saccharomyces boulardii cell wall polysaccharide (group Ⅲ), milk replacer+0.3% Saccharomyces boulardii cell wall polysaccharide (group Ⅳ) and milk replacer+0.5% Saccharomyces boulardii cell wall polysaccharide (group Ⅴ), respectively. The experiment lasted for 44 days, including transition period, preliminary period and test period, and they were designed to 8, 7 and 29 days, respectively. The results showed as follows:1) the fecal index of groups Ⅳ and Ⅴ was significantly lower than that of group Ⅰ by 20.73% and 15.85% (P<0.05), respectively; the diarrhea rate of groups Ⅳ and Ⅴ was significantly lower than that of group Ⅰ by 31.34% and 23.75% (P<0.05). 2) The secreted immunoglobulin A (sIgA) content in colonic mucosa of groups Ⅳ and Ⅴ was significantly higher than that of group Ⅰ by 14.49% and 17.77% (P<0.05), respectively; the immunoglobulin A (IgA) content in colonic mucosa of group Ⅲ was significantly higher than that of group I by 5.81% (P<0.05); the interleukin-1 (IL-1) content in colonic mucosa of groups Ⅳ and Ⅴ was significantly lower than that of group I by 7.93% and 10.58% (P<0.05), respectively; the interleukin-10 (IL-10) content in colonic mucosa of group Ⅳ was significantly higher than that of group Ⅰ by 8.05% (P<0.05). Moreover, the immunoglobulin G (IgG) content increased and the tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) decreased in colonic mucosa of groups Ⅳ and Ⅴ compared with group Ⅰ, but the differences were not significant (P>0.05). The above results indicate that adding 0.3% to 0.5% Saccharomyces boulardii cell wall polysaccharide in the milk replacer can decrease the diarrhea rate of early-weaned lambs in a certain degree, and enhance the intestinal mucosal immune level.

Key words： Saccharomyces boulardii cell wall polysaccharide; diarrhea rate; intestinal immunity; early-weaned lambs

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