动物营养学报 >

2020 , Vol. 32 >Issue 9: 4251 - 4259

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.035

水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

饲料中添加γ-氨基丁酸对凡纳滨对虾生长性能、血清生化指标和抗亚硝酸氮应激能力的影响

  • 赵红霞 ,
  • 陈晓瑛 ,
  • 王国霞 ,
  • 彭凯 ,
  • 黄燕华 ,
  • 陈冰 ,
  • 曹俊明
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  • 1. 广东省农业科学院动物科学研究所, 广州 510640;
    2. 农业农村部华南动物营养与饲料重点实验室, 广州 510640;
    3. 广东省畜禽育种与营养研究重点实验室, 广州 510640
赵红霞(1976-),女,内蒙古乌海人,研究员,博士,从事水产动物营养与饲料的研究。E-mail:zhaohongxia8866@163.com

收稿日期: 2020-03-25

  网络出版日期: 2020-09-17

基金资助

国家自然科学基金项目（31402307）；广东省现代农业产业技术体系建设项目（2019KJ115）；科技创新战略专项资金（高水平农科院建设）（201614TD）

收起

Effects of Dietary γ-Aminobutyric Acid Supplemental Level on Growth Performance, Serum Biochemical Indices and Anti-Nitrite-Nitrogen Stress Ability of Litopenaeus vannamei

  • ZHAO Hongxia ,
  • CHEN Xiaoying ,
  • WANG Guoxia ,
  • PENG Kai ,
  • HUANG Yanhua ,
  • CHEN Bing ,
  • CAO Junming
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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangzhou 510640, China;
    3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2020-03-25

  Online published: 2020-09-17

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摘要

本试验旨在探讨饲料中添加GABA对凡纳滨对虾（Litopenaeus vannamei）生长性能、血清生化指标和抗亚硝酸氮应激能力的影响。将906尾初始均重为（0.68±0.02）g的凡纳滨对虾幼虾随机分为6组，每组4个重复，每个重复放养40尾虾。6组对虾分别投喂添加0、50、100、150、200和250 mg/kg γ-氨基丁酸（GABA）的等氮等脂试验饲料56 d。饲养试验结束后进行120 h的亚硝酸氮应激。结果显示：1）与未添加组相比，饲料中添加50~250 mg/kg GABA显著提高了凡纳滨对虾的摄食率和增重率（P<0.05）。2）与未添加组相比，饲料中添加100和150 mg/kg GABA显著提高了凡纳滨对虾血清总蛋白、胆固醇、甘油三酯含量（P<0.05），显著降低了血清谷丙转氨酶、谷草转氨酶活性（P<0.05）。3）100和150 mg/kg GABA组凡纳滨对虾血清溶菌酶、超氧化物歧化酶、过氧化氢酶活性和总抗氧化能力显著高于未添加组（P<0.05）。4）与未添加组相比，饲料中添加100~250 mg/kg GABA显著降低了凡纳滨对虾亚硝酸氮应激120 h时的累积死亡率（P<0.05），其中100 mg/kg GABA组凡纳滨对虾累积死亡率达到最低。综上可知，饲料中添加GABA能够提高凡纳滨对虾的摄食率、增重率和抗亚硝酸氮应激能力。以增重率为评价指标，通过二次回归分析得出凡纳滨对虾饲料中GABA的适宜添加量为154.79 mg/kg。

关键词： &gamma; -氨基丁酸; 凡纳滨对虾; 生长性能; 血清生化指标; 亚硝酸氮应激

本文引用格式

赵红霞 , 陈晓瑛 , 王国霞 , 彭凯 , 黄燕华 , 陈冰 , 曹俊明 . 饲料中添加γ-氨基丁酸对凡纳滨对虾生长性能、血清生化指标和抗亚硝酸氮应激能力的影响[J]. 动物营养学报, 2020 , 32(9) : 4251 -4259 . DOI: 10.3969/j.issn.1006-267x.2020.09.035

Abstract

This experiment was conducted to investigate the effects of dietary γ-aminobutyric acid (GABA) supplemental level on growth performance, serum biochemical indices and anti-nitrite-nitrogen stress ability of Litopenaeus vannamei. Six isonitrogenous and isolipidic diets were formulated to contain graded levels of GABA (0, 50, 100, 150, 200 and 250 mg/kg of diet), by adding GABA. Nine hundred and sixty Litopenaeus vannamei shrimp with an initial average body weight of (0.68±0.02) g were randomly divided into 6 groups with 4 replicates of 40 shrimp each and each group was fed with one of the diets. After 56 d feeding, shrimp were exposed to nitrite-nitrogen for 120 h. The results showed as follows:1) the weight gain rate (WGR) and feed intake (FI) of shrimp fed diet with 50 to 250 mg/kg GABA were significantly higher than those of shrimp fed diet without GABA (P<0.05). 2) Compared with the no-adding group, diet with 100 and 150 mg/kg GABA significantly increased the contents of serum total protein (TP), triglyceride (TG) and cholesterol (CHO) (P<0.05), and significantly decreased the activities of serum glutamic oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) (P<0.05). 3) The serum lysozyme (LYZ), superoxide dismutase (SOD), catalase (CAT) activities and total antioxidant capacity (T-AOC) in 100 and 150 mg/kg GABA groups were significantly higher than those in no-adding group (P<0.05). 4) After 120 h challenge to nitrite-nitrogen stress, the cumulative mortality rate in 100, 150, 200 and 250 mg/kg GABA groups was significantly lower than that in no-adding group (P<0.05). In conclusion, dietary supplemented with GABA can improve the FI, WGR and anti-nitrite-nitrogen stress ability of Litopenaeus vannamei. Using WGR as the evaluation index, the optimal dietary GABA supplemental level is estimated to be 154.79 mg/kg of diet by quadratic regression analysis.

Key words： γ-aminobutyric acid; Litopenaeus vannamei; growth performance; serum biochemical indices; nitrite-nitrogen stress

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