动物营养学报 >

2020 , Vol. 32 >Issue 9: 4366 - 4375

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.047

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

蒸汽调质制粒的采样过程对植酸酶活性残留率测定的影响

  • 吴宇轩 ,
  • 赵峰 ,
  • 苏艳芳 ,
  • 张虎 ,
  • 李浙烽 ,
  • 张倩云 ,
  • 赵威 ,
  • 贺喜
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  • 1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    2. 湖南农业大学动物科技学院, 长沙 410128;
    3. 杭州康德权饲料有限公司, 包膜饲料添加剂省级重点农业企业研究院, 杭州 311107
吴宇轩(1997-),男,湖南株洲人,硕士研究生,从事饲料养分生物学效价评定的研究。E-mail:914575943@qq.com

收稿日期: 2020-03-27

  网络出版日期: 2020-09-17

基金资助

中国农业科学院科技创新工程（ASTIP-IAS07）；杭州康德权饲料有限公司与中国农业科学院北京畜牧兽医研究所合作项目（2017-YF-05）

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Effects of Sampling Procedure on Determination of Residual Rate of Phytase Activity in Steam Conditioning-Pelleting Process

  • WU Yuxuan ,
  • ZHAO Feng ,
  • SU Yanfang ,
  • ZHANG Hu ,
  • LI Zhefeng ,
  • ZHANG Qianyun ,
  • ZHAO Wei ,
  • HE Xi
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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science, Hunan Agricultural University, Changsha 410128, China;
    3. Provincial Key Agricultural Enterprise Research Institute of Encapsulated Feed Additive, King Techina Technology Co., Ltd., Hangzhou 311107, China

Received date: 2020-03-27

  Online published: 2020-09-17

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摘要

本试验旨在考察蒸汽调质制粒过程中不同采样时间区域间样品中植酸酶活性的变异对残留率测定的影响。采用单因素完全随机设计，待蒸汽调质制粒稳定后在调质前、调质后、制粒后、冷却后4个位点每2 min采集1 min样品。每个采样位点采集30个样品。以20 min内10个样品为1个采样时间区域，共计21个时间区域（如0~18 min、2~20 min）。测定调质制粒过程中各位点样品的植酸酶活性，计算各采样时间区域内植酸酶活性的残留率，比较代表性采样区间内植酸酶活性平均值与10个样品合并后植酸酶活性测定值的差异。结果表明：在调质温度达75℃稳定后的0~58 min采样区间，沿着调质-制粒-冷却工艺过程，饲粮中植酸酶活性依次从39.8、28.3、20.1 U/g降低到14.7 U/g，调质后和冷却后饲粮中植酸酶活性波动较大（变异系数≥ 21.9%）。在0~18 min、20~38 min和40~58 min 3个无重叠样品组间，调质后0~18 min植酸酶的平均活性显著低于20~38 min的样品平均值（P<0.05）。调质-制粒-冷却后0~18 min和20~38 min植酸酶的平均活性均显著高于40~58 min的样品平均值（P<0.05）。在采样区间的12~30 min、14~32 min、16~34 min和18~36 min饲粮中植酸酶活性平均值、植酸酶活性的残留率均与全程平均值最为接近。在采样区间的14~32 min、16~34 min和18~36 min中，每个区域每个采样位点10个饲粮样品植酸酶活性的平均值与该区域10个样品合并后实测值部分存在显著性差异（P<0.05），但相对偏差都在8%以内。综上所述，在调质制粒稳定后的12~18 min开始采样，每2 min采集1 min样品，共采集10个样品的植酸酶活性平均值可以代表全程取样样品的平均值。

关键词： 调质制粒; 采样; 植酸酶; 残留率

本文引用格式

吴宇轩 , 赵峰 , 苏艳芳 , 张虎 , 李浙烽 , 张倩云 , 赵威 , 贺喜 . 蒸汽调质制粒的采样过程对植酸酶活性残留率测定的影响[J]. 动物营养学报, 2020 , 32(9) : 4366 -4375 . DOI: 10.3969/j.issn.1006-267x.2020.09.047

Abstract

The objective of this study was to investigate the effects of variation in phytase activity of samples collected from different time zones in steam conditioning-pelleting on the determination of residual rate of phytase. A complete random design was adopted. When the steam conditioning-pelleting was stable, samples were collected for 1 min every 2 min at 4 sites of anterior conditioning, posterior conditioning, pelleting or cooling. Thirty samples were collected at each sampling site. A sampling time zone was defined with 10 samples continuously collected within 20 min, thus there were 21 sampling time zones (such as 0 to 18 min, 2 to 20 min). The phytase activity was determined for each sample collected from each site in steam conditioning-pelleting. The residual rates of phytase activity were calculated for each sampling time zone. The average phytase activity in the representative sampling time zones was compared with determined phytase activity of the pooled 10 samples. The results showed that in the sampling time zone of 0 to 58 min after the conditioning temperature was stable at 75℃, the phytase activity of diets decreased from 39.8, 28.3, 20.1 to 14.7 U/g in sequence along the conditioning-pelleting-cooling process. The phytase activity of diets fluctuated greatly after conditioning and cooling (CV ≥ 21.9%). In 3 non-overlapping sample time zones of 0 to 18 min, 20 to 38 min and 40 to 58 min, the average phytase activity of samples collected from 0 to 18 min was significantly lower than that of samples collected from 20 to 38 min after conditioning (P<0.05). The average phytase activities of samples collected from 0 to 18 min or 20 to 38 min after conditioning-pelleting-cooling were significantly greater than that of samples collected from 40 to 58 min (P<0.05). In the sampling time zones of 12 to 30 min, 14 to 32 min, 16 to 34 min, and 18 to 36 min, the average phytase activity and residual rate of phytase activity were close to the average value of full samples. In each sampling time zones of 14 to 32 min, 16 to 34 min and 18 to 36 min, significant differences were observed between average phytase activity in 10 samples and determined values of that in pooled 10 samples from some collection sites (P<0.05), but the relative differences were less than 8%. In conclusion, after the conditioning temperature is stable for 12 to 18 min, the average phytase activity of 10 samples collected for 1 min every 2 min can represent the mean value of full diet samples in the steam condition-pelleting process.

Key words： conditioning-pelleting; sampling; phytase; residual rate

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