动物营养学报 >

2020 , Vol. 32 >Issue 9: 4376 - 4385

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.048

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

鸭油甘油二酯与维生素K1协同对葡聚糖硫酸钠诱导的小鼠溃疡性结肠炎损伤的修复作用

  • 丛红霞 ,
  • 王宝维 ,
  • 葛文华 ,
  • 张名爱 ,
  • 王茜 ,
  • 徐慧心 ,
  • 凡文磊 ,
  • 孔敏
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  • 1. 青岛农业大学食品科学与工程学院, 青岛 266109;
    2. 国家水禽产业技术体系营养与饲料功能研究室, 青岛 266109
丛红霞(1995-),女,山东潍坊人,硕士研究生,研究方向为营养与保健。E-mail:912737843@qq.com

收稿日期: 2020-03-14

  网络出版日期: 2020-09-17

基金资助

山东省2018年度农业重大应用技术创新项目；国家水禽产业技术体系专项基金（CARS-42-14）；国家重点研发计划"绿色水禽高效安全养殖技术应用与示范"（2018YFD0501501）

收起

Repairing Effects of Duck Oil Diglyceride Combined with Vitamin K1 on Dextran Sodium Sulfate-Induced Ulcerative Colitis Injury in Mice

  • CONG Hongxia ,
  • WANG Baowei ,
  • GE Wenhua ,
  • ZHANG Ming'ai ,
  • WANG Qian ,
  • XU Huixin ,
  • FAN Wenlei ,
  • KONG Min
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  • 1. Department of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China;
    2. National Waterfowl Industry Technical System Nutrition and Feed Function Laboratory, Qingdao 266109, China

Received date: 2020-03-14

  Online published: 2020-09-17

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摘要

本试验旨在研究鸭油甘油二酯（DDG）与维生素K1协同对葡聚糖硫酸钠（DSS）诱导的小鼠溃疡性结肠炎（UC）损伤的修复作用。试验分为2个阶段。造模阶段：将250只小鼠随机分为2个组，对照组每组5个重复，每个重复10只；炎症模型组每组20个重复，每个重复10只。对照组小鼠自由饮用蒸馏水，炎症模型组小鼠均自由饮用3% DSS水溶液诱导小鼠肠道炎症，连续7 d。修复干预阶段：将160只小鼠随机分成对照组（Ⅰ组）、炎症模型组（Ⅱ组）和修复组（Ⅲ~Ⅷ组），每组4个重复，每个重复5只。Ⅰ、Ⅱ组以蒸馏水灌胃（5.00 mL/kg BW），Ⅲ~Ⅷ组给予不同水平的DDG和维生素K1灌胃，DDG添加水平分别为2.50、3.75、5.00 mL/kg BW，维生素K1添加水平分别为1.00、2.00 mg/kg BW。试验期7 d。结果表明：1）炎症模型组小鼠的造模后体重和结肠长度显著低于对照组（P<0.05），血清肿瘤坏死因子-α（TNF-α）和白细胞介素（IL）-6含量显著高于对照组（P<0.05），表明DSS诱导的UC小鼠模型造模成功。2）Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ组修复后体重和结肠长度显著高于Ⅱ组（P<0.05），Ⅳ组血清髓过氧化物酶（MPO）活性显著低于Ⅱ、Ⅲ、Ⅶ、Ⅷ组（P<0.05）。DDG与维生素K1协同对UC小鼠的血清MPO活性的交互作用显著（P<0.05）。3）DDG与维生素K1协同对UC小鼠血清炎症细胞因子（IL-1β、IL-4、IL-6、IL-17、TNF-α、IL-10）含量的交互作用显著（P<0.05）。4）Ⅳ、Ⅵ组的结肠绒毛高度显著高于Ⅱ、Ⅲ、Ⅶ、Ⅷ组（P<0.05），Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ组的结肠隐窝深度显著低于Ⅱ组（P<0.05），Ⅵ组的结肠绒毛高度/隐窝深度显著高于Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅶ、Ⅷ组（P<0.05）。DDG与维生素K1协同对UC小鼠结肠绒毛高度和绒毛高度/隐窝深度的交互作用显著（P<0.05）。5）Ⅷ组的肝脏维生素K1含量组显著高于Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ组（P<0.05）。DDG与维生素K1协同对UC小鼠肝脏维生素K1含量的交互作用不显著（P>0.05）。由此可见，DDG与维生素K1协同可有效减少UC小鼠炎症细胞因子的释放，促进肠道组织的修复发育；DDG能促进UC小鼠肝脏中维生素K1的吸收，加快对肠炎的止血修复。小鼠按3.75 mL/kg BW的DDG和1.00 mg/kg BW的维生素K1灌胃对UC损伤的修复效果最佳。

关键词： 鸭油甘油二酯; 维生素K1; 溃疡性结肠炎; 修复

本文引用格式

丛红霞 , 王宝维 , 葛文华 , 张名爱 , 王茜 , 徐慧心 , 凡文磊 , 孔敏 . 鸭油甘油二酯与维生素K1协同对葡聚糖硫酸钠诱导的小鼠溃疡性结肠炎损伤的修复作用[J]. 动物营养学报, 2020 , 32(9) : 4376 -4385 . DOI: 10.3969/j.issn.1006-267x.2020.09.048

Abstract

This experiment was conducted to study the repairing effects of duck oil diglyceride (DDG) combined with vitamin K1 on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) injury in mice. The experiment was divided into two phases. Modeling phase:a total of 250 mice were randomly divided into 2 groups, the control group contained 5 replicates per group and 10 mice per replicate, and the inflammatory model group contained 20 replicates per group and 10 mice per replicate. Mice in the control group were free to drink distilled water, and the others in inflammatory model group were free to drink 3% DSS aqueous solution to induce the intestinal inflammation of mice, which lasted for 7 days. Repair intervention phase:a total of 160 mice were randomly divided into control group (group Ⅰ), inflammatory model group (group Ⅱ) and repair groups (groups Ⅲ to Ⅷ), and each group contained 4 replicates and 5 mice per replicate. Mice in groups Ⅰ and Ⅱ were gavage with distilled water (5.00 mL/kg BW); mice in groups Ⅲ to Ⅷ were gavage with different levels of DDG and vitamin K1, and the DDG supplemental levels were 52.50, 3.75 and 5.00 mL/kg BW, respectively; and the vitamin K1 supplemental levels were 1.00 and 2.00 mg/kg BW, respectively. The experiment lasted for 7 days. The results showed as follows:1) the body weight after modeling and colonic length of mice of inflammatory model group were significantly lower than those of the control group (P<0.05), and the contents of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in serum were significantly higher than those of the control group (P<0.05), it showed that the ulcerative colitis mice model successful build. 2) The body weight after repairing and colonic length of groups Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅶ and Ⅷ were significantly higher than those of group Ⅱ (P<0.05), the serum myeloperoxidase (MPO) activity of group Ⅳ was gnificantly lower than that of groups Ⅱ, Ⅲ, Ⅶ and Ⅷ (P<0.05). DDG combined with vitamin K1 had a significant interaction on serum MPO activity of UC mice (P<0.05). 3) DDG combined with vitamin K1 had significant interaction on serum inflammatory cytokine (IL-1β, IL-4, IL-6, IL-17, TNF-α and IL-10) contents of UC mice (P<0.05). 4) The colonic villus height of groups Ⅳ and Ⅵ was gnificantly higher than that of groups Ⅱ, Ⅲ, Ⅶ and Ⅷ (P<0.05), the colonic crypt depth of groups Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅶ and Ⅷ was gnificantly lower than that of group Ⅱ (P<0.05), and the colonic villus height/crypt depth of group Ⅵ was gnificantly higher than that of groups Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅶ and Ⅷ (P<0.05). DDG combined with vitamin K1 had significant interaction on colonic villus height and villus height/crypt depth of UC mice (P<0.05). 5) The liver vitamin K1 content of group Ⅷ was gnificantly higher than that of groups Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ (P<0.05). DDG combined with vitamin K1 had significant interaction on liver vitamin K1 content of UC mice (P<0.05). In conclusion, DDG combined with vitamin K1 can effectively reduced the release of inflammatory cytokines, promote the repair and development of intestinal tissues; DDG can promote the absorption of vitamin K1 in liver of UC mice, accelerate the hemostasis and repair of enteritis. The mice gavage with 3.75 mL/kg BW DDG and 1.00 mg/kg BW vitamin K1 have the best repair effect on UC injury.

Key words： duck oil diacylglycerol; vitamin K1; ulcerative colitis; repair

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