动物营养学报 >

2020 , Vol. 32 >Issue 9: 4408 - 4417

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.09.052

研究简报 SHORT COMMUNICATION

核苷酸对凡纳滨对虾低蛋白质水平饲料中营养物质表观消化率的影响

  • 陈晓瑛 ,
  • 赵红霞 ,
  • 王国霞 ,
  • 曹俊明 ,
  • 孙育平
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  • 1. 广东省农业科学院动物科学研究所, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640;
    2. 广州飞禧特生物科技有限公司, 广州 510640
陈晓瑛(1987-),女,福建漳平人,助理研究员,硕士,主要从事水产动物营养与饲料学研究。E-mail:524852502@qq.com

收稿日期: 2020-03-27

  网络出版日期: 2020-09-17

基金资助

广东省促进经济发展专项资金海洋经济发展项目（GD0E[2019]A22）；科技创新战略专项资金（高水平农科院建设）（201614TD）；广东省农业科学院院长基金项目（201806）

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Effects of Nucleotides on Nutrient Apparent Digestibility in Low Protein Level Diet of Litopenaeus vannamei

  • CHEN Xiaoying ,
  • ZHAO Hongxia ,
  • WANG Guoxia ,
  • CAO Junming ,
  • SUN Yuping
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  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    2. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China

Received date: 2020-03-27

  Online published: 2020-09-17

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摘要

本试验旨在研究核苷酸对凡纳滨对虾饲料中干物质、粗蛋白质、粗脂肪、粗灰分、总磷、氨基酸和脂肪酸表观消化率的影响。选取初始体质量为（2.00±0.01）g的凡纳滨对虾480尾，随机分成3组，每组4个重复，每个重复40尾。设置2个对照组，即低蛋白质水平对照组（L0组，饲料粗蛋白质水平为37.92%）和高蛋白质水平对照组（H0组，饲料粗蛋白质水平为46.45%），并在L0组基础上添加400 mg/kg的核苷酸混合物（LN组）。自正式投喂试验饲料后的第10天开始，采用虹吸法连续收集粪便30 d。结果显示：1）LN组的干物质、粗蛋白质、粗脂肪、粗灰分和总磷表观消化率最高。LN组的粗脂肪和总磷表观消化率显著高于H0组（P<0.05），但与L0组无显著差异（P>0.05）。2）各组之间的各氨基酸、非必需氨基酸和必需氨基酸表观消化率均无显著差异（P>0.05）。3）LN组的饱和脂肪酸、C17：0、C20：0、C22：1、C18：3、二十二碳六烯酸（DHA）表观消化率显著高于L0组和H0组（P<0.05）。LN组的C14：0、C16：1表观消化率显著高于H0组（P<0.05），但与L0组无显著差异（P>0.05）。各组之间的单不饱和脂肪酸、多不饱和脂肪酸和总脂肪酸表观消化率均无显著差异（P>0.05）。由此可见，在低蛋白质水平饲料中添加核苷酸具有改善凡纳滨对虾对饲料营养物质表观消化率的效果，特别是对粗脂肪、总磷和脂肪酸。

关键词： 核苷酸; 凡纳滨对虾; 低蛋白质水平饲料; 营养物质表观消化率

本文引用格式

陈晓瑛 , 赵红霞 , 王国霞 , 曹俊明 , 孙育平 . 核苷酸对凡纳滨对虾低蛋白质水平饲料中营养物质表观消化率的影响[J]. 动物营养学报, 2020 , 32(9) : 4408 -4417 . DOI: 10.3969/j.issn.1006-267x.2020.09.052

Abstract

This experiment was aiming to study the effects of nucleotides on apparent digestibilities of dry matter, crude protein, ether extract, phosphorus, amino acids and fatty acids of Litopenaeus vannamei. A total of 480 Litopenaeus vannamei with initial weight of (2.00±0.01) g were randomly divided into 3 groups with 4 replicates per group and 40 fish per replicate. Two control groups were set, which were low protein control group (L0 group, dietary crude protein level was 37.92%) and high protein control group (H0 group, dietary crude protein level was 46.45%), and supplemented with 400 mg/kg nucleotides based on L0 group (LN group). From the 10th day after feeding the formal experimental diets, and the feces were collected continuously by siphon method for 30 days. The results showed as follows:1) the apparent digestibilities of dry matter, crude protein, ether extract, ash and total phosphorus of LN group were the highest. The apparent digestibilities of ether extract and total phosphorus of LN group were significantly higher than those of the H0 group (P<0.05), but there were no significant differences with the L0 group (P>0.05). 2) There were no significant differences on apparent digestibilities of each amino acids, nonessential amino acids and essential amino acids among all groups (P>0.05). 3) The apparent digestibilities of saturated fatty acids C17:0, C20:0, C22:1, C18:3 and docosahexaenoic acid (DHA) of LN group were significantly higher than those of L0 group and H0 group (P<0.05). The apparent digestibilities of C14:0 and C16:1 of LN group were significantly higher than those of the H0 group (P<0.05), but there were no significant differences with the L0 group (P>0.05). There were no significant differences on apparent digestibilities of monounsaturated fatty acids, polyunsaturated fatty acids and total fatty acids among all groups (P>0.05). In conclusion, adding nucleotides in low protein level diets can improve the nutrient apparent digestibilities of diets of Litopenaeus vannamei, especially for the ether extract, total phosphorus and fatty acids.

Key words： nucleotides; Litopenaeus vannamei; low protein level diets; nutrient apparent digestibilities

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