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不同精粗比饲粮条件下复合益生菌对绵羊瘤胃微生物种群的影响

  • 谢明欣 ,
  • 李常瑞 ,
  • 杨晓东 ,
  • 石彩霞 ,
  • 王海荣
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  • 内蒙古农业大学动物科学学院, 内蒙古自治区高校动物营养与饲料科学重点实验室, 呼和浩特 010018
谢明欣(1992-),女,辽宁鞍山人,博士研究生,研究方向为动物营养与饲料科学。E-mail:xiemingxin1112@163.com

收稿日期: 2020-03-19

  网络出版日期: 2020-09-17

基金资助

国家自然科学基金项目(31860658);内蒙古科技计划项目(KCBJ2018009)

Effects of Compound Probiotics on Rumen Microbial Population in Sheep Fed Different Concentrate to Roughage Ratio Diets

  • XIE Mingxin ,
  • LI Changrui ,
  • YANG Xiaodong ,
  • SHI Caixia ,
  • WANG Hairong
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  • Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2020-03-19

  Online published: 2020-09-17

摘要

本试验旨在研究在不同精粗比饲粮条件下复合益生菌对绵羊典型瘤胃微生物种群的影响。试验选取6只装有瘤胃瘘管的成年蒙古绵羊,按体重差异不显著(P>0.05)原则分为2组,采用交叉试验方法设计,试验分为2期进行:第1期,2组绵羊分别饲喂精粗比为3:7和7:3的饲粮,同时投喂复合益生菌(酿酒酵母1号、克鲁斯假丝酵母、东方伊萨酵母、酿酒酵母3号、热带假丝酵母2号、枯草芽孢杆菌);第2期,2组绵羊饲粮互换,饲喂方式相同。每期试验预试期15 d,正试期25 d,2期间隔21 d。结果表明:与饲喂复合益生菌1 d相比,在低精料饲粮水平条件下,饲喂复合益生菌10 d可以显著提高瘤胃溶纤维丁酸弧菌、黄色瘤胃球菌数量(P<0.05),饲喂复合益生菌20 d可以显著提高反刍兽新月形单胞菌、溶纤维丁酸弧菌、黄色瘤胃球菌、白色瘤胃球菌和总产甲烷菌数量(P<0.05);在高精料饲粮水平条件下,饲喂复合益生菌10 d可以显著降低反刍兽新月形单胞菌、白色瘤胃球菌和总产甲烷菌数量(P<0.05),饲喂复合益生菌20 d显著降低白色瘤胃球菌数量(P<0.05),但显著高于饲喂复合益生菌10 d时的数量(P<0.05)。在本试验条件下,不同精粗比饲粮中添加相同的复合益生菌对有益菌群的影响趋势不同;从作用效果看,该复合益生菌在精粗比为3:7的饲粮中起到的益生作用较好。

本文引用格式

谢明欣 , 李常瑞 , 杨晓东 , 石彩霞 , 王海荣 . 不同精粗比饲粮条件下复合益生菌对绵羊瘤胃微生物种群的影响[J]. 动物营养学报, 2020 , 32(9) : 4418 -4428 . DOI: 10.3969/j.issn.1006-267x.2020.09.053

Abstract

The objective of this study was to investigate the effects of compound probiotics on rumen typical microbial populations of sheep fed with different concentrate to roughage ratio diets. Six adult Mongolian sheep with rumen fistulas were randomly divided into two groups. Cross-over experiment design was used. The experiment contained two periods. In the period 1, 3:7 or 7:3 concentrate to roughage ratio of diets which both supplemented with compound probiotics were fed two groups, respectively. In period 2, the two groups exchanged the diets. Each period lasted for 40 days, including a 15-day adaptation and a 25-day experimentation. The interval between the two periods was 21 days. The results showed as follows:compared with sheep fed compound compound probiotics for 1 d, under low-concentration diet condition, the number of Butyrivibrio fibrisolvens and Ruminococcus flavefaciens significantly increased by feeding probiotics for 10 days (P<0.05), the number of Selenomonas ruminantium, Butyrivibrio fibrisolvens, Ruminococcus flavefaciens, Ruminococcus albus and total methanogens significantly increased by feeding compound probiotics for 20 days (P<0.05); under high-concentrate diet condition, the number of Selenomonas ruminantium, Ruminococcus albus and total methanogens significantly decreased by feeding compound probiotics for 10 days (P<0.05). Moreover, the number of Ruminococcus albus significantly decreased by feeding compound probiotics for 20 days (P<0.05) but was higher than feeding that for 10 days (P<0.05). It is concluded that different concentrate to roughage ratio diets supplemented with the same compound probiotics has different effects on the beneficial flora; and the compound probiotic has a batter probiotic impact when supplied to 3:7 diets.

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