水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

大菱鲆胆汁和血浆中的胆汁酸轮廓分析

  • 姚婷 ,
  • 韦娴 ,
  • 薛敏 ,
  • 孙宝忠 ,
  • 谷旭 ,
  • 张松山
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  • 1. 中国农业科学院北京畜牧兽医研究所, 北京 100032;
    2. 北京市饲料监察所, 北京 100061;
    3. 中国农业科学院饲料研究所, 农业农村部动物产品质量安全饲料源性因子风险评估实验室, 北京 100081
姚婷(1990-),女,山西洪洞人,中级畜牧师,博士研究生,从事农产品质量安全研究。E-mail:yaoting0515@126.com

收稿日期: 2020-05-27

  网络出版日期: 2020-12-07

基金资助

蓝色粮仓科技创新重点专项(2018YFD0900400,2019YFD090020)

Contour Identification and Analysis of Bile Acids in Bile and Plasma of Scophthalmus maximus L.

  • YAO Ting ,
  • WEI Xian ,
  • XUE Min ,
  • SUN Baozhong ,
  • GU Xu ,
  • ZHANG Songshan
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  • 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100032, China;
    2. Beijing Institute of Feed Control, Beijing 100061, China;
    3. Laboratory of Quality & Safety Risk Assessment for Animal Products of China Ministry of Agriculture and Rural Affairs, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2020-05-27

  Online published: 2020-12-07

摘要

本试验旨在解析大菱鲆胆汁和血浆中的胆汁酸谱,明确2种组织中胆汁酸种类和含量,为鱼类胆汁酸相关研究提供技术支撑和数据依据。研究利用超高效液相色谱-串联质谱技术,配合内标校正,建立了鱼胆汁和血浆中30种胆汁酸的定性定量方法,并对大菱鲆胆汁和血浆进行了胆汁酸谱分析。该方法灵敏度、准确度和精密度均能够满足生物样品中胆汁酸的分析要求。结果表明:在大菱鲆胆汁中检测到6种初级胆汁酸和4种次级胆汁酸,其中初级胆汁酸种类按其含量高低排列分别为牛磺胆酸(TCA)、牛磺鹅去氧胆酸(TCDCA)、胆酸(CA)、甘氨鹅脱氧胆酸(GCDCA)、甘氨胆酸(GCA)和鹅去氧胆酸(CDCA),次级胆汁酸按其含量高低排列分别为牛磺β鼠胆酸(TβMCA)、牛磺ω鼠胆酸(TωMCA)、葡糖石胆酸(GLCA)和甘氨鹅胆酸(GHCA)。在大菱鲆血浆中检测到较低含量的5种初级胆汁酸和3种次级胆汁酸。其中初级胆汁酸的种类有CA、CDCA、GCDCA、TCA、TCDCA,次级胆汁酸为鼠胆酸(ωMCA)和TωMCA。本研究首次在鱼体内发现了ωMCA的存在,并为其他鱼类胆汁酸轮廓鉴定分析提供了参考方法,丰富了鱼类胆汁酸谱库,为后续利用胆汁酸进行相关研究提供数据依据。

本文引用格式

姚婷 , 韦娴 , 薛敏 , 孙宝忠 , 谷旭 , 张松山 . 大菱鲆胆汁和血浆中的胆汁酸轮廓分析[J]. 动物营养学报, 2020 , 32(12) : 5816 -5826 . DOI: 10.3969/j.issn.1006-267x.2020.12.035

Abstract

In this study, the bile acids (BAs) in bile and plasma of Scophthalmus maximus L.,were analyzed which focused on the species and contents of BAs, in order to support technical support and data basis for relevant studies of BAs in fish. In this study, ultra-liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was utilized for qualitative and quantitative analysis of 30 kinds of BAs in bile and plasma with the assistant of internal standard. The method could match the requirements of BAs analysis in biological samples with good sensitivity, accuracy and precision. The results showed as follows: 6 kinds of primary BAs and 4 kinds of secondary BAs were detected in bile, among which the primary BAs were taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), cholic acid (CA), glycochenodeoxycholic acid (GCDCA), glycocholic acid (GCA) and chenodeoxycholic acid (CDCA), arranged in order respectively. The secondary BAs were arranged in order of content level, namely, tauro-beta-muricholic acid (TβMCA), tauro-omega-muricholic acid (TωMCA), glycolithocholic acid (GLCA) and glycohyocholic acid (GHCA). In plasma, low contents of 5 primary BAs and 3 secondary BAs were discovered. The primary BAs were CA, CDCA, GCDCA, TCA and TCDCA, and the secondary BAs were murine cholic acids (ωMCA) and TωMCA. In this study, murine cholic acids are found in fish for the first time. This study, which can enrich the fish BAs spectrum library, can be a reference method for the contour identification and analysis of other fish BAs, and provide the data basis for subsequent studies on BAs.

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