动物营养学报 >

2020 , Vol. 32 >Issue 12: 5884 - 5892

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.041

特种经济动物营养与饲料 SPECIAL ECONOMIC ANIMAL NUTRITION AND FEED

不同铜源对冬毛期乌苏里貉生产性能、血清生化指标及肝脏相关基因表达的影响

  • 张新宇 ,
  • 张海华 ,
  • 刘晗璐 ,
  • 钟伟 ,
  • 韩菲菲 ,
  • 王静 ,
  • 穆琳琳 ,
  • 陈双双 ,
  • 李光玉
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  • 1. 中国农业科学院特产研究所, 特种经济动物分子生物学重点实验室, 长春 130112;
    2. 河北科技师范学院动物科技学院, 河北省特色动物种质资源挖掘与创新重点实验室, 秦皇岛 066004
张新宇(1995-),男,内蒙古赤峰人,硕士研究生,动物营养与饲料科学专业。E-mail:zhangxinyu951105@163.com

收稿日期: 2020-05-07

  网络出版日期: 2020-12-07

基金资助

中国农业科学院创新工程项目（CAAS-ASTIP-2019-ISAPS）；河北省青年拔尖人才项目（180443）；河北省高校百名优秀创新人才项目（SLRC2019048）；河北省自然科学基金面上项目（C2019407111）

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Effects of Different Copper Sources on Performance, Serum Biochemical Indexes and Liver Related Gene Expression of Raccoon Dogs in Winter Fur-Growing Period

  • ZHANG Xinyu ,
  • ZHANG Haihua ,
  • LIU Hanlu ,
  • ZHONG Wei ,
  • HAN Feifei ,
  • WANG Jing ,
  • MU Linlin ,
  • CHEN Shuangshuang ,
  • LI Guangyu
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  • 1. State Key Laboratory of Molecular Biology of Special Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
    2. Hebei Key Laboratory of Specialty Animal Germplasm Resources Innovation and Utilization, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China

Received date: 2020-05-07

  Online published: 2020-12-07

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摘要

本试验旨在研究不同铜源对冬毛期乌苏里貉生产性能、脏器指数、血清生化指标及肝脏相关基因表达的影响。随机选取（135±5）日龄、健康的雄性乌苏里貉60只，随机分为4组，每组15个重复，每个重复1只。在基础饲粮中分别添加硫酸铜（Ⅰ组）、碱式氯化铜（Ⅱ组）、混合铜（Ⅲ组，碱式氯化铜和蛋氨酸铜，添加比例为1：1，以铜元素计）和蛋氨酸铜（Ⅳ组），添加水平均为60 mg/kg。每组选取8只动物进行采血与屠宰试验。预试期7 d，正试期60 d。结果表明：1）Ⅰ组末重显著低于其余各组（P<0.05），Ⅳ组针毛长显著高于Ⅰ、Ⅱ组（P<0.05）。2）不同铜源对脏器指数没有显著影响（P>0.05）。3）Ⅰ、Ⅱ组血清葡萄糖（GLU）含量显著低于Ⅲ、Ⅵ组（P<0.05），Ⅱ、Ⅲ、Ⅵ组血清铜蓝蛋白（CER）活性显著高于Ⅰ组（P<0.05），Ⅲ、Ⅵ组血清总超氧化物歧化酶（T-SOD）活性显著高于Ⅰ组（P<0.05），Ⅵ组血清铜锌超氧化物歧化酶（Cu-Zn SOD）活性显著高于Ⅰ组（P<0.05）。4）Ⅳ组肝脏CER基因相对表达量显著高于Ⅰ组（P<0.05）。Ⅲ、Ⅳ组肝脏生长激素受体（GHR）基因相对表达量显著高于Ⅰ组（P<0.05）。Ⅲ、Ⅳ组肝脏胰岛素生长因子-Ⅰ（IGF-Ⅰ）基因相对表达量显著高于Ⅰ组（P<0.05）。Ⅲ、Ⅳ组肝脏Cu-Zn SOD基因相对表达量显著高于Ⅰ、Ⅱ组（P<0.05）。在本试验条件下，饲粮中添加蛋氨酸铜和混合铜可以显著提升冬毛期乌苏里貉的体重、血清CER与Cu-Zn SOD活性及肝脏GHR、IGF-Ⅰ、CER与Cu-Zn SOD基因相对表达量；结合实际生产，建议生产中有机铜与无机铜混合使用效果更好。

关键词： 铜; 乌苏里貉; 超氧化物歧化酶; 铜蓝蛋白

本文引用格式

张新宇 , 张海华 , 刘晗璐 , 钟伟 , 韩菲菲 , 王静 , 穆琳琳 , 陈双双 , 李光玉 . 不同铜源对冬毛期乌苏里貉生产性能、血清生化指标及肝脏相关基因表达的影响[J]. 动物营养学报, 2020 , 32(12) : 5884 -5892 . DOI: 10.3969/j.issn.1006-267x.2020.12.041

Abstract

This experiment was conducted to study the effects of different copper sources on performance, organ indexes, serum biochemical indexes and liver related genes expression of raccoon dogs in winter fur-growing period. Sixty healthy male raccoon dogs at the age of (135±5) days with the similar body weight were randomly divided into 4 groups with 15 replicates each and 1 raccoon dog per replicate. The raccoon dogs in the 4 groups were fed the basal diet supplemented with 60 mg/kg copper from copper sulfate (group Ⅰ), basic copper chloride (group Ⅱ) and mixed copper (group Ⅲ, the ratio of basic copper chloride to copper methionine was 1:1) and copper methionine (group Ⅳ). The pre-test period lasted for 7 days and the trial period lasted for 60 days.Eight animals in each group were selected for blood collection and slaughter test. The results showed as follows: 1) the body weight in group Ⅰ was significantly lower than that in other groups (P<0.05), the guard hair long in the group Ⅳ was significantly longer than that in the groups Ⅰ and Ⅱ (P<0.05). 2) There was no significant difference in organ indexes among groups (P>0.05). 3) The serum glucose (GLU) content in groups Ⅲ and Ⅳ was significantly higher than that in the groups Ⅰ and Ⅱ (P<0.05). The serum ceruloplasmin (CER) content in the groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that in the group Ⅰ (P<0.05). The serum total superoxide dismutase (T-SOD) activity in groups Ⅲ and Ⅳ was significantly higher than that in the group Ⅰ (P<0.05). The serum copper and zinc superoxide dismutase (Cu-Zn SOD) activity in group Ⅳ was significantly higher than that in the group Ⅰ (P<0.05) 4) The relative expression level of CER gene in group Ⅳ was significantly higher than that in group Ⅰ (P<0.05). The relative expression level of growth hormone receptor (GHR) gene in groups Ⅲ and Ⅳ was significantly higher than that in group Ⅰ (P<0.05). The relative expression level of insulin-like growth factor-Ⅰ (IGF-Ⅰ) gene in groups Ⅲ and Ⅳ was significantly higher than that in group Ⅰ (P<0.05). The relative expression level of Cu-Zn SOD gene in groups Ⅲ and Ⅳ was significantly higher than that in groups Ⅰ and Ⅱ (P<0.05). Under the condition of this experiment, copper methionine and mixed copper groups significantly increase the body weight, serum CER and Cu-Zn SOD activities, and significantly increase the relative expression levels of GHR, IGF-Ⅰ, CER and Cu-Zn SOD genes. Combined with actual production, it is suggested that organic copper and inorganic copper should be mixed in production.

Key words： copper; raccoon dog; SOD; CER

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