动物营养学报 >

2020 , Vol. 32 >Issue 12: 5925 - 5931

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.12.046

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

双连续型党参多糖纳米乳对免疫抑制小鼠的免疫调节作用

  • 时菲菲 ,
  • 王妲妲 ,
  • 曹金花 ,
  • 于生兰
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  • 江苏农牧科技职业学院, 动物药学院, 泰州 225300
时菲菲(1985-),女,内蒙古赤峰人,讲师,硕士,从事药物制剂与药物分析研究。E-mail:faye.shi@qq.com

收稿日期: 2020-05-20

  网络出版日期: 2020-12-07

基金资助

江苏省高等学校自然科学面上研究项目（19KJD230002）；江苏农牧科技职业学院科研项目（NSF201805）

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Immunomodulatory Effects of W/O/W Codonopsis pillosula Polysaccharide Nanoemulsion on Immunosuppressed Mice

  • SHI Feifei ,
  • WANG Dada ,
  • CAO Jinhua ,
  • YU Shenglan
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  • College of Veterinary Medicine, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China

Received date: 2020-05-20

  Online published: 2020-12-07

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摘要

本试验旨在研究双连续型（W/O/W）党参多糖纳米乳（CPPS-NE）对免疫抑制小鼠的免疫调节作用。将320只小鼠分成4个单元，每个单元80只。将每个单元的80只小鼠随机分为空白对照组、模型对照组、CPPS-NE高、中、低剂量组和党参多糖水溶液（CPPS）高、中、低剂量组，每组10只（雌雄各占1/2）。空白对照组和模型对照组以0.2 mL生理盐水灌胃；CPPS-NE高、中、低剂量组分别以0.3、0.2、0.1 mL CPPS-NE灌胃，剂量分别为900、600、300 mg/kg BW，CPPS高、中、低剂量组分别以0.3、0.2、0.1 mL CPPS灌胃，剂量分别为900、600、300 mg/kg BW。连续灌胃14 d，1次/d，除空白对照组外，其余小鼠皮下注射氢化可的松建立免疫抑制模型。第1单元测定碳粒廓清指数；第2单元测定小鼠胸腺和脾脏指数；第3单元测定血清溶血素含量；第4单元测定血清细胞因子含量，包括肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、白细胞介素-12（IL-12）含量，分析CPPS-NE对非特异性免疫、体液免疫和细胞免疫的免疫调节作用。结果表明：与模型对照组对比，CPPS-NE可显著提高免疫器官指数（P<0.05），显著增强单核巨噬细胞吞噬能力（P<0.05），有效促进溶血素合成（P<0.05），显著提高血清中的TNF-α、IFN-γ、IL-2、IL-6、IL-10、IL-12细胞因子含量（P<0.05），呈明显量效依赖关系，且CPPS-NE作用效果明显强于同剂量CPPS。综上所述，党参多糖对免疫抑制小鼠的非特异性免疫、体液免疫和细胞免疫具有免疫调节作用，将党参多糖制备成纳米乳制剂可显著增强作用效果。

关键词： 党参多糖; 纳米乳; 免疫抑制; 免疫调节

本文引用格式

时菲菲 , 王妲妲 , 曹金花 , 于生兰 . 双连续型党参多糖纳米乳对免疫抑制小鼠的免疫调节作用[J]. 动物营养学报, 2020 , 32(12) : 5925 -5931 . DOI: 10.3969/j.issn.1006-267x.2020.12.046

Abstract

This experiment was conducted to investigate the immunoregulation effects of W/O/W Codonopsis pillosula polysaccharide nanoemulsion (CPPS-NE) on immunosuppressed mice. A total of 320 healthy mice were dividied into 4 units, and each unit had 80 mice. The 80 mice in each unit were randomly divided into a blank control group, a model control group, high-, medium- and low-dose CPPS-NE groups, high-, medium- and low-dose Codonopsis pillosula polysaccharide solution(CPPS) groups, and each group had 10 mice (half male and half femal). The blank control group and the model control group were given 0.2 mL normal saline by intragastric administration. The high-, medium- and low-dose CPPS-NE groups were gavaged 0.3, 0.2 and 0.1 mL of 900, 600 and 300 mg/kg BW CPPS-NE, respectively, while the high-, medium- and low-dose CPPS groups were gavaged 0.3, 0.2 and 0.1 mL of 900, 600 and 300 mg/kg BW CPPS, respectively. All mice were given with normal saline, CPPS-NE or CPPS by intragastric administration for 14 days, once a day. In addition to the blank control group, other mice were subcutaneously injected with hydrocortisone to obtain immunosuppressive model. The unit 1 detected carbon clearance index, the unit 2 deteced thymus and spleen indexes, the unit 3 detected serum hemolysin content, and the unit 4 detected serum cytokines contents, including tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-10 (IL-10) and interleukin-12 (IL-12) in mice. The results showed that, compared with the model control group, CPPS-NE could significantly improve the immune organ index (P<0.05), phagocytic ability of monocyte macrophages (P<0.05), effectively promote hemolysin synthesis (P<0.05), and significantly increase the contents of TNF-α, IFN-γ, IL-2, IL-6, IL-10 and IL-12 in serum (P<0.05), which was dose-dependent. Moreover, the effect of CPPS-NE was significantly stronger than that of CPPS in the same dose group. The results show that Codonopsis pillosula polysaccharide has immunomodulatory effects on nonspecific immunity, humoral immunity and cellular immunity of immunosuppressed mice, and CPPS-NE can significantly enhance the effect.

Key words： Codonopsis pillosula polysaccharide; nanoemulsion; immunosuppression; immunoregulation

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