本试验旨在探索应用聚酯网袋测定饲料中中性洗涤纤维（NDF）和酸性洗涤纤维（ADF）含量的可行性。本试验采用国标法、聚酯网袋法、Ankom纤维分析仪法和聚酯网袋仪器法4种方法，分别测定了17种饲料中NDF和ADF的含量，比较不同方法的测定结果。结果表明：1）4种方法对饲料中NDF和ADF含量的测定结果基本一致，两两配对分析差异不显著（P>0.05）。2）聚酯网袋法与国标法对NDF含量的测定结果基本一致，2种方法的测定结果呈强正相关：y=0.998 3x+0.130 5（x为国标法测定的饲料中NDF含量，y为聚酯网袋法测定的饲料中NDF含量），n=17，r=0.999 7，P<0.01。3）聚酯网袋法与国标法对ADF含量的测定结果基本一致，2种方法测定结果呈强正相关：y=0.999 0x-0.034 0（x为国标法测定的饲料中ADF含量，y为聚酯网袋法测定的饲料中ADF含量），n=17，r=0.999 9，P<0.01。综上可知，聚酯网袋法测定饲料中NDF和ADF含量的方法具有测定时间短、效率高、成本低、精密度高等特点，可以替代国标法。聚酯网袋可以替代Ankom F57滤袋使用Ankom纤维分析仪测定饲料中NDF和ADF含量。

The objective of this study was to evaluate the feasibility of the determination of neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents in feed using polyester mesh bag. Four different methods, including national standard method, polyester mesh bag method, Ankom fiber analyzer method and polyester mesh bag instrument method, were utilized to determine the contents of NDF and ADF contents from 17 different feeds, respectively, and a comprehensive comparison was conducted to evaluate the differentiation of the results among the different methods. The results showed as follows: 1) there were no significant differences in the contents of ADF and NDF in feeds that determined by the four methods (P>0.05). 2) The content of NDF determined by the polyester mesh bag method was similar to that determined by the national standard method, and the determination results of the two methods exhibited a strong positive correlation: y=0.998 3x+0.130 5(x was the content of NDF determined by the national standard method, and y was the content of NDF determined by the polyester mesh bag method), n=17, r=0.999 7, P<0.01. 3) The content of ADF determined by the polyester mesh bag method was similar to that determined by national standard method, and the determination results of the two methods presented a strong positive correlation: y=0.999 0x-0.034 0 (x was the content of ADF determined by the national standard method, and y was the content of ADF determined by the polyester mesh bag method), n=17, r=0.999 9, P<0.01. In conclusion, the polyester mesh bag method for determining the contents of NDF and ADF contents in feed has some advantages such as shorter analysis time, higher efficiency, lower cost, and improved precision, thus it can replace the national standard method. In addition, the Ankom F57 filter-bag can be replaced by polyester mesh bag when using the Ankom fiber analyzer to determine the contents of NDF and ADF contents in feed.