综述 REVIEW

牛乳腺上皮细胞体外分离培养模式、方法及其应用进展

  • 母童 ,
  • 虎红红 ,
  • 顾亚玲 ,
  • 张娟
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  • 宁夏大学农学院, 银川 750105
母童(1995-),男,宁夏银川人,博士研究生,从事动物遗传育种与繁殖研究。E-mail:mtlv3h@163.com

收稿日期: 2020-06-16

  网络出版日期: 2021-01-18

基金资助

宁夏自治区农业育种专项优质高产奶牛选育资助项目(2019NYYZ05)

Isolation and Culture Mode, Method and Application of Bovine Mammary Epithelial Cells in Vitro

  • MU Tong ,
  • HU Honghong ,
  • GU Yaling ,
  • ZHANG Juan
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  • Agricultural College, Ningxia University, Yinchuan 750105, China

Received date: 2020-06-16

  Online published: 2021-01-18

摘要

乳腺由具有泌乳功能的腺泡组成,乳腺上皮细胞(MEC)以单层方式排列在腺泡外围,是乳腺对外界病原进行免疫保护的重要组分,负责将血液中的营养物质通过一系列复杂生化过程转化为乳汁。牛乳腺上皮细胞(BMECs)的体外分离培养在很大程度上解决了活体试验条件不可控、操作困难、成本高及个体差异大等诸多问题,还可以为体外研究乳腺组织生长发育规律、泌乳机制、乳房疾病等提供良好的细胞模型。本文总结了BMECs的分类和作用、培养方法、模式及其在基因表达机理研究和组学中的应用进展,以期为BMECs体外培养及相关研究提供有价值的参考和新思路。

关键词: ; BMECs; 体外培养; 模式; 应用

本文引用格式

母童 , 虎红红 , 顾亚玲 , 张娟 . 牛乳腺上皮细胞体外分离培养模式、方法及其应用进展[J]. 动物营养学报, 2021 , 33(1) : 56 -67 . DOI: 10.3969/j.issn.1006-267x.2021.01.006

Abstract

The mammary gland is composed of acini with lactation function. Mammary epithelial cells (MECs) are arranged in a single layer around the acinus, which is an important part of the innate immune barrier of the mammary gland. It is also responsible for transforming nutrients in the blood into milk through a series of complex biochemical processes. The separation and culture of bovine mammary epithelial cells (BMECs) in vitro can solve many problems, such as uncontrollable conditions, difficult operation, high cost and large individual differences in vivo test, and also provide a good cell model for in vitro study of the growth and development of mammary tissue, the mechanism of lactation and breast diseases. This paper mainly summarized the classification and function of BMECs, culture methods, mode and their application in gene expression mechanism and omics, in order to provide valuable reference and new ideas for the in vitro culture and related research of BMECs.

参考文献

[1] NAYLOR M J,OAKES S R,GARDINER-GARDEN M,et al.Transcriptional changes underlying the secretory activation phase of mammary gland development[J].Molecular Endocrinology,2005,19(7):1868-1883.  
[2] 林杰,王旭荣,李建喜,等.奶牛乳腺上皮细胞原代培养、纯化及鉴定技术研究进展[J].中国畜牧兽医,2015,42(8):2109-2115. LIN J,WANG X R,LI J X,et al.Research progress on primary culture,purification and identification technique of bovine mammary epithelial cell[J].China Animal Husbandry & Veterinary Medicine,2015,42(8):2109-2115.(in Chinese)
[3] HARVATINE K J,BOISCLAIR Y R,BAUMAN D E.Liver x receptors stimulate lipogenesis in bovine mammary epithelial cell culture but do not appear to be involved in diet-induced milk fat depression in cows[J].Physiological Reports,2014,2(3):e00266.
[4] KAUSHIK R,SINGH K P,KUMARI A,et al.Construction of a recombinant human insulin expression vector for mammary gland-specific expression in buffalo (Bubalus bubalis) mammary epithelial cell line[J].Molecular Biology Reports,2014,41(9):5891-5902.  
[5] SUN Y L,LIN C S,CHOU Y C.Gene transfection and expression in a primary culture of mammary epithelial cells isolated from lactating sows[J].Cell Biology International,2013,29(7):576-582.
[6] MEYER M J,CAPUCO A V,ROSS D A,et al.Developmental and nutritional regulation of the prepubertal bovine mammary gland:Ⅱ.Epithelial cell proliferation,parenchymal accretion rate,and allometric growth[J].Journal of Dairy Science,2006,89(11):4298-4304.  
[7] CAPUCO A V,ELLIS S E,HALE S A,et al.Lactation persistency:insights from mammary cell proliferation studies[J].Journal of Animal Science,2003,15(Suppl.3):13-15.
[8] DONTU G,AL-HAJJ M,ABDALLAH W M,et al.Stem cells in normal breast development and breast cancer[J].Breast Cancer Research,2003,36(Suppl.1):59-72.
[9] HENNIGHAUSEN L,ROBINSON G W.Signaling pathways in mammary gland development[J].Developmental Cell,2001,1(4):467-475.  
[10] MARTIGNANI E,ACCORNERO P,MIRETTI S,et al.Bovine mammary organoids:a model to study epithelial mammary cells[M]//BARATTA M.Epithelial cell culture.New York:Humana Press,2018,1817:137.
[11] CUI Y J,LIU Z Y,SUN X,et al.Thyroid hormone responsive protein spot 14 enhances lipogenesis in bovine mammary epithelial cells[J].In Vitro Cellular & Developmental Biology-Animal,2015,51(6):586-594.  
[12] GREEN K A,STREULI C H.Apoptosis regulation in the mammary gland[J].Cellular and Molecular Life Sciences,2004,61(15):1867-1883.  
[13] BARNES D,SATO G.Serum-free cell culture:a unifying approach[J].Cell,1980,22(3):649-655.  
[14] MARTIGNANI E,CRAVERO D,MIRETTI S,et al.Clonogenic assay allows for selection of a primitive mammary epithelial cell population in bovine[J].Experimental Cell Research,2015,338(2):245-250.  
[15] CAPUCO A V,ELLIS S,WOOD D L,et al.Postnatal mammary ductal growth:three-dimensional imaging of cell proliferation,effects of estrogen treatment,and expression of steroid receptors in prepubertal calves[J].Tissue and Cell,2002,34(3):143-154.  
[16] JAMIESON P R,DEKKERS J F,RIOS A C,et al.Derivation of a robust mouse mammary organoid system for studying tissue dynamics[J].Development,2016,144(6):1065-1071.
[17] RUDLAND P S,BARRACLOUGH R,FERNIG D G,et al.Growth and differentiation of the normal mammary gland and its tumours[J].Biochemical Society Symposium,1998,63:1-20.
[18] MIN K H,BYUN J H,LIM J S,et al.Chondroid syringoma on face[J].Archives of Craniofacial Surgery,2016,17(3):173-175.  
[19] CARTER E P,GOPSILL J A,GOMM J J,et al.A 3D in vitro model of the human breast duct:a method to unravel myoepithelial-luminal interactions in the progression of breast cancer[J].Breast Cancer Research,2017,19(1):50.
[20] URANO M,NAKAGURO M,YAMAMOTO Y,et al.Diagnostic significance of HRAS mutations in epithelial-myoepithelial carcinomas exhibiting a broad histopathologic spectrum[J].American Journal of Surgical Pathology,2019,43(7):984-994.  
[21] 宫平,刘凤华.奶牛乳腺上皮细胞的原代培养[J].北京农学院学报,2017,32(1):48-50. GONG P,LIU F H.Primary culture of bovine mammary epithelial cells[J].Journal of Beijing University of Agriculture,2017,32(1):48-50.(in Chinese)
[22] HOU X M,LI Q Z,HUANG T Y.Microarray analysis of gene expression profiles in the bovine mammary gland during lactation[J].Science China Life Sciences,2010,53(2):248-256.  
[23] SORG D,DANOWSKI K,KORENKOVA V,et al.Microfluidic high-throughput RT-qPCR measurements of the immune response of primary bovine mammary epithelial cells cultured from milk to mastitis pathogens[J].Animal,2013,7(5):799-805.  
[24] AHN J Y,AOKI N,ADACHI T,et al.Isolation and culture of bovine mammary epithelial cells and establishment of gene transfection conditions in the cells[J].Bioscience,Biotechnology,and Biochemistry,2014,59(1):59-64.
[25] XU W W,CHEN Q M,JIA Y H,et al.Isolation,characterization,and SREBP1 functional analysis of mammary epithelial cell in buffalo[J].Journal of Food Biochemistry,2019,43(11):e12997.
[26] TONG H L,LI Q Z,GAO X J,et al.Establishment and characterization of a lactating dairy goat mammary gland epithelial cell line[J].In Vitro Cellular & Developmental Biology-Animal,2012,48(3):149-155.  
[27] SHI H P,SHI H B,LUO J,et al.Establishment and characterization of a dairy goat mammary epithelial cell line with human telomerase (hT-MECs)[J].Animal Science Journal,2014,85(7):735-743.  
[28] 林杰,王旭荣,王磊,等.荷斯坦奶牛乳腺组织冻存及乳腺上皮细胞原代培养技术改进[J].畜牧兽医学报,2016,47(5):1067-1074. LIN J,WANG X R,WANG L,et al.Cryopreservation of the mammary gland and improvement on the culture of the primary epithelial cells in holstein dairy cows[J].Acta Veterinaria et Zootechnica Sinica,2016,47(5):1067-1074.(in Chinese)
[29] JEDRZEJCZAK M,SZATKOWSKA I.Bovine mammary epithelial cell cultures for the study of mammary gland functions[J].In Vitro Cellular & Developmental Biology-Animal,2014,50(5):389-398.  
[30] LU C Y,YANG R J,LIU B Y,et al.Establishment of two types of mammary epithelial cell lines from Chinese Holstein dairy cow[J].Journal of Animal and Veterinary Advances,2012,11(8):1166-1172.  
[31] 林桂娟,王恬,陈才勇.机械破碎法分离奶牛乳腺上皮细胞的体外培养研究[J].畜牧与兽医,2004,36(7):4-6. LIN G J,WANG T,CHEN C Y.A study on primary culture of cow mammary epithelial cell separated directly[J].Animal Husbandry and Veterinary Medicine,2004,36(7):4-6.(in Chinese)
[32] GERMAN T,BARASH I.Characterization of an epithelial cell line from bovine mammary gland[J].In Vitro Cellular & Developmental Biology-Animal,2002,38(5):282-292.  
[33] ANAND V,DOGRA N,SINGH S,et al.Establishment and characterization of a buffalo (Bubalus bubalis) mammary epithelial cell line[J].PLoS One,2012,7(7):e40469.
[34] CHEN Q M,WU Y J,ZHANG M Y,et al.Milk fat globule is an alternative to mammary epithelial cells for gene expression analysis in buffalo[J].Journal of Dairy Research,2016,83(2):202-208.  
[35] ROSE M T,ASO H,YONEKURA S,et al.In vitro differentiation of a cloned bovine mammary epithelial cell[J].Journal of Dairy Research,2002,69(3):345-355.  
[36] HAN H,WANG J Q,BU D P,et al.In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow[J].PLoS One,2009,4(11):e7636.
[37] HÉBERT A,SAYASITH K,SÉNÉCHAL S,et al.Demonstration of intracellular Staphylococcus aureus in bovine mastitis alveolar cells and macrophages isolated from naturally infected cow milk[J].FEMS Microbiology Letters,2001,193(1):57-62.
[38] BHAT S A,AHMAD S M,IBEAGHA-AWEMU E M,et al.Comparative transcriptome analysis of mammary epithelial cells at different stages of lactation reveals wide differences in gene expression and pathways regulating milk synthesis between Jersey and Kashmiri cattle[J].PLoS One,2019,14(2):e0211773.
[39] 李艳,徐凯,赵国琦.奶牛乳腺上皮细胞的培养与鉴定[J].上海畜牧兽医通讯,2014(1):8-11. LI Y,XU K,ZHAO G Q.Culture and identification of cow mammary epithelial cells[J].Shanghai Animal Husbandry and Veterinary Journal,2014(1):8-11.(in Chinese)
[40] 张燕,杜卫华,郝海生,等.哺乳动物乳腺上皮细胞体外培养研究进展[J].中国畜牧兽医,2011,38(10):34-38. ZHANG Y,DU W H,HAO H S,et al.Progress of mammary epithelial cells culture in vitro[J].China Animal Husbandry and Veterinary Medicine,2011,38(10):34-38.(in Chinese)
[41] SUN Y L,LIN C S,CHOU Y C.Establishment and characterization of a spontaneously immortalized porcine mammary epithelial cell line[J].Cell Biology International,2006,30(12):970-976.  
[42] CHOUDHARY R K,CAPUCO A V.In vitro expansion of the mammary stem/progenitor cell population by xanthosine treatment[J].BMC Cell Biology,2012,13:14.
[43] 丁旭娜,王凤龙,丁玉林,等.奶牛乳腺上皮细胞和成纤维细胞的体外培养与鉴定[J].黑龙江畜牧兽医,2012(3):1-5. DING X N,WANG F L,DING Y L,et al.Identification and in vitro culture of bovine mammary epithelial cells and fibroblast[J].Heilongjiang Animal Science and Veterinary Medicine,2012(3):1-5.(in Chinese)
[44] 李震,王英,刘惠莉,等.牛乳腺上皮细胞的分离培养[J].上海农业学报,2000,16(3):25-28. LI Z,WANG Y,LIU H L,et al.Study on isolation and culture of bovine mammary epithelial cells[J].Acta Agriculturae Shanghai,2000,16(3):25-28.(in Chinese)
[45] ZAVIZION B,VAN DUFFELEN M,SCHAEFFER W,et al.Establishment and characterization of a bovine mammary epithelial cell line with unique properties[J].In Vitro Cellular & Developmental Biology-Animal,1996,32(3):138-148.  
[46] BUEHRING G C.Culture of human mammary epithelial cells:keeping abreast with a new method[J].Journal of the National Cancer Institute,1972,49(5):1433-1434.
[47] RAUNER G,BARASH I.Xanthosine administration does not affect the proportion of epithelial stem cells in bovine mammary tissue,but has a latent negative effect on cell proliferation[J].Experimental Cell Research,2014,328(1):186-196.  
[48] HAN Z Y,MU T,YANG Z.Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells[J].Cell Stress and Chaperones,2015,20(1):109-120.  
[49] KAUSHIK R,SINGH K P,KUMARI A,et al.Isolation,characterization,and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line[J].In Vitro Cellular & Developmental Biology-Animal,2013,49(1):1-7.
[50] FU M,CHEN Y B,XIONG X R,et al.Establishment of mammary gland model in vitro:culture and evaluation of a yak mammary epithelial cell line[J].PLoS One,2014,9(12):e113669.
[51] TONG J J,LI Y,LIU R,et al.Effect of Semen vaccariae and Taraxacu mogono on cell adhesion of bovine mammary epithelial cells[J].Journal of Integrative Agriculture,2012,11(12):2043-2050.  
[52] NEVILLE M C,MCFADDEN T B,FORSYTH I.Hormonal regulation of mammary differentiation and milk secretion[J].Journal of Mammary Gland Biology and Neoplasia,2002,7(1):49-66.  
[53] 段安琴,庞春英,朱鹏,等.水牛乳汁中乳腺上皮细胞的分离培养及鉴定[J].中国畜牧兽医,2017,44(11):3243-3249. DUAN A Q,PANG C Y,ZHU P,et al.Isolation,culture and identification of buffalo mammary epithelial cells from milk[J].China Animal Husbandry and Veterinary Medicine,2017,44(11):3243-3249.(in Chinese)
[54] AKERS R M.Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows[J].Journal of Dairy Science,2006,89(4):1222-1234.  
[55] WU W,CHEN Y H,UEDA E,et al.Different forms of prolactin have opposing effects on the expression of cell cycle regulatory proteins in differentiated mammary epithelial cells[J].Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics,2006,16(2):75-84.  
[56] 郝明超,刘犇,樊江峰,等.乳腺上皮细胞体外培养的研究进展[J].中国奶牛,2011(14):39-43. HAO M C,LIU B,FAN J F,et al.Research progress on culture of mammary gland epithelial cells in vitro[J].China Dairy Cattle,2011(14):39-43.(in Chinese)
[57] FEUERMANN Y,MABJEESH S J,SHAMAY A.Leptin affects prolactin action on milk protein and fat synthesis in the bovine mammary gland[J].Journal of Dairy Science,2004,87(9):2941-2946.  
[58] ACCORNERO P,LUVARÀ S,FAVOLE A,et al.Biological role of the HGF/MET ligand/receptor couple in bovine mammary epithelial cells[J].Veterinary Research Communications,2007,31(Suppl.1):161-164.
[59] CAO L,TANG J Y,LI Q,et al.Expression of selenoprotein genes is affected by heat stress in IPEC-J2 cells[J].Biological Trace Element Research,2016,172(2):354-360.  
[60] ZWOLAK I,ZAPOROWSKA H.Selenium interactions and toxicity:a review[J].Cell Biology and Toxicology,2012,28(1):31-46.  
[61] MIRANDA S G,PURDIE N G,OSBORNE V R,et al.Selenomethionine increases proliferation and reduces apoptosis in bovine mammary epithelial cells under oxidative stress[J].Journal of Dairy Science,2011,94(1):165-173.  
[62] ZOU Y X,SHAO J J,LI Y X,et al.Protective effects of inorganic and organic selenium on heat stress in bovine mammary epithelial cells[J].Oxidative Medicine and Cellular Longevity,2019,2019:1-10.
[63] LIN Y,SUN X X,HOU X M,et al.Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow[J].BMC Veterinary Research,2016,12:81.
[64] 王秀美,考桂兰,高爱武,等.奶牛乳腺上皮细胞三维培养的形态观察[J].内蒙古农业大学学报(自然科学版),2009,30(4):8-14. WANG X M,KAO G L,GAO A W,et al.The three-dimensional cultures of bovine mammary epithelial cells[J].Journal of Inner Mongolia Agricultural University (Natural Science Edition),2009,30(4):8-14.(in Chinese)
[65] XU K,BUCHSBAUM R J.Isolation of mammary epithelial cells from three-dimensional mixed-cell spheroid co-culture[J].Journal of Visualized Experiments,2012(62):3760.
[66] 徐丹丹,孙志鹏,张旭,等.胶原酶Ⅰ消化法体外分离培养奶牛乳腺上皮细胞[J].中国细胞生物学学报,2016,38(9):1060-1065. XU D D,SUN Z P,ZHANG X,et al.Isolation and culture of bovine mammary epithelial cells by collagenase I digestion in vitro[J].Chinese Journal of Cell Biology,2016,38(9):1060-1065.(in Chinese)
[67] 欧阳五庆,钱菊汾.山羊乳腺上皮细胞培养体系的建立[J].西北农林科技大学学报(自然科学版),2003,31(3):31-34. OU YANG W Q,QIAN J F.Establishment of culture system in goat mammary epithelial cell[J].Journal of Northwest A&F University (Natural Science Edition),2003,31(3):31-34.(in Chinese)
[68] 刘畅,赵锋,李庆章.不同泌乳相关激素和生长因子对奶牛乳腺上皮细胞增殖的影响[J].中国畜牧兽医,2012,39(5):102-106. LIU C,ZHAO F,LI Q Z.Influence of different hormone and cytokine related lactation on the proliferation of mammary epithelial cells of dairy cow[J].China Animal Husbandry and Veterinary Medicine,2012,39(5):102-106.(in Chinese)
[69] OHTANI Y,YONEZAWA T,SONG S H,et al.Gene expression and hormonal regulation of adiponectin and its receptors in bovine mammary gland and mammary epithelial cells[J].Animal Science Journal,2011,82(1):99-106.  
[70] YU C,SHI Z R,CHU C Y,et al.Expression of bovine granulocyte chemotactic protein-2(GCP-2) in neutrophils and a mammary epithelial cell line (MAC-T) in response to various bacterial cell wall components[J].The Veterinary Journal,2010,186(1):89-95.  
[71] REZAMAND P,MCGUIRE M A.Short communication:effects of trans fatty acids on markers of inflammation in bovine mammary epithelial cells[J].Journal of Dairy Science,2011,94(1):316-320.  
[72] YAMADA K M,CUKIERMAN E.Modeling tissue morphogenesis and cancer in 3D[J].Cell,2007,130(4):601-610.  
[73] DEBNATH J,MUTHUSWAMY S K,BRUGGE J S.Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures[J].Methods,2003,30(3):256-268.  
[74] HSIAO A Y,TUNG Y C,KUO C H,et al.Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates[J].Biomedical Microdevices,2012,14(2):313-323.  
[75] ZHAN K,LIN M,LIU M M,et al.Three-dimensional culture system can induce expression of casein in immortalized bovine mammary epithelial cells[J].Animal Science Journal,2017,88(5):817-825.  
[76] LEE G Y,KENNY P A,LEE E H,et al.Three-dimensional culture models of normal and malignant breast epithelial cells[J].Nature Methods,2007,4(4):359-365.  
[77] MROUE R,BISSELL M J.Three-dimensional cultures of mouse mammary epithelial cells[M]//RANDELL S,FULCHER M.Epithelial cell culture protocols.Totowa,NJ:Humana Press,2012,945:221-250.
[78] HILLREINER M,MÜLLER N I,KOCH H M,et al.Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk[J].In Vitro Cellular & Developmental Biology-Animal,2017,53(8):706-720.  
[79] 邢媛媛,李大彪,李子南,等.不同浓度亚油酸对二维和三维培养模式下奶牛乳腺上皮细胞形态及乳蛋白合成相关基因表达的影响[J].动物营养学报,2019,31(10):4667-4674. XING Y Y,LI D B,LI Z N,et al.Effects of different concentrations of linoleic acid on expression of milk protein synthesis related gene in two-dimension and three-dimension cultured bovine mammary epithelial cells[J].Chinese Journal of Animal Nutrition,2019,31(10):4667-4674.(in Chinese)
[80] LARSEN M C,BRAKE P B,POLLENZ R S,et al.Linked expression of ah receptor,ARNT,CYP1A1,and CYP1B1 in rat mammary epithelia,in vitro,is each substantially elevated by specific extracellular matrix interactions that precede branching morphogenesis[J].Toxicological Sciences,2004,82(1):46-61.  
[81] OGAWA R,OKI K,HYAKUSOKU H.Vascular tissue engineering and vascularized 3D tissue regeneration[J].Regenerative Medicine,2007,2(5):831-837.  
[82] SODUNKE T R,TURNER K K,CALDWELL S A,et al.Micropatterns of matrigel for three-dimensional epithelial cultures[J].Biomaterials,2007,28(27):4006-4016.  
[83] 李喜艳,王加启,魏宏阳,等.奶牛乳腺上皮细胞的体外培养方法及其应用概述[J].华北农学报,2010,25(增刊2):31-35. LI X Y,WANG J Q,WEI H Y,et al.Bovine mammary epithelial cells culture methods in vitro and its application[J].Acta Agriculturae Boreali-Sinica,2010,25(Suppl.2):31-35.(in Chinese)
[84] 韩立强,孙宇,付彤,等.奶牛乳腺上皮细胞中SCAP和SREBP1蛋白调控SCD基因表达的作用研究[J].畜牧兽医学报,2018,49(3):652-658. HAN L Q,SUN Y,FU T,et al.Effect of SCAP and SREBP1 proteins on regulation of SCD gene expression in dairy mammary epithelial cells[J].Journal of Animal Husbandry and Veterinary Science,2018,49(3):652-658.(in Chinese)
[85] WEN G,PACHNER L I,GESSNER D K,et al.Sterol regulatory element-binding proteins are regulators of the sodium/iodide symporter in mammary epithelial cells[J].Journal of Dairy Science,2016,99(11):9211-9226.  
[86] LI P,YU M M,ZHOU C J,et al.FABP5 is a critical regulator of methionine- and estrogen-induced SREBP-1c gene expression in bovine mammary epithelial cells[J].Journal of Cellular Physiology,2019,234(1):537-549.  
[87] KADEGOWDA A K G,BIONAZ M,PIPEROVA L S,et al.Peroxisome proliferator-activated receptor-γ activation and long-chain fatty acids alter lipogenic gene networks in bovine mammary epithelial cells to various extents[J].Journal of Dairy Science,2009,92(9):4276-4289.  
[88] CHEN S,HU Z G,HE H,et al.Fatty acid elongase7 is regulated via SP1 and is involved in lipid accumulation in bovine mammary epithelial cells[J].Journal of Cellular Physiology,2017,233(6):4715-4725.
[89] LU C Y,YANG R J,SHEN B L,et al.RNA interference-mediated knockdown of DGAT1 decreases triglyceride content of bovine mammary epithelial cell line[J].Gene Expression,2013,15(5/6):199-206.
[90] WANG F G,ZHAO Y,CHEN S X,et al.Astragaloside Ⅳ alleviates ammonia-induced apoptosis and oxidative stress in bovine mammary epithelial cells[J].International Journal of Molecular Sciences,2019,20(3):600.
[91] CHEN Q,YANG W,WANG X X,et al.TGF-β1 induces EMT in bovine mammary epithelial cells through the TGFβ1/smad signaling pathway[J].Cellular Physiology and Biochemistry,2017,43(1):82-93.  
[92] ZHANG W Y,HAO W,QI S P,et al.CYP1A1 relieves lipopolysaccharide-induced inflammatory responses in bovine mammary epithelial cells[J].Mediators of Inflammation,2018,2018:4093285.
[93] MUROYA S,HAGI T,KIMURA A,et al.Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture[J].Journal of Animal Science and Biotechnology,2016,7:8.
[94] SHEN B L,ZHANG L Y,LIAN C J,et al.Deep sequencing and screening of differentially expressed microRNAs related to milk fat metabolism in bovine primary mammary epithelial cells[J].International Journal of Molecular Sciences,2016,17(2):200.
[95] WANG H,WANG X X,LI X R,et al.A novel long non-coding RNA regulates the immune response in MAC-T cells and contributes to bovine mastitis[J].The FEBS Journal,2019,286(9):1780-1795.  
[96] MA M R,PEI Y F,WANG X X,et al.LncRNA XIST mediates bovine mammary epithelial cell inflammatory response via NF-κB/NLRP3 inflammasome pathway[J].Cell Proliferation,2018,52(1):e12525.
[97] XU T,DONG Z J,WANG X X,et al.IL-1β induces increased tight junction permeability in bovine mammary epithelial cells via the IL-1β-ERK1/2-MLCK axis upon blood-milk barrier damage[J].Journal of Cellular Biochemistry,2018,119(11):9028-9041.  
[98] LI L,WANG Y R,LI C M,et al.Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells[J].Animal Science Journal,2017,88(12):2090-2099.  
[99] CAI X Y,LIU Q Y,ZHANG X X,et al.Identification and analysis of the expression of microRNA from lactating and nonlactating mammary glands of the Chinese swamp buffalo[J].Journal of Dairy Science,2017,100(3):1971-1986.  
[100] GOLAN-GERST R,SHIFF Y E,MOSHAYOFF V,et al.Characterization and biological function of milk-derived miRNAs[J].Molecular Nutrition & Food Research,2017,61(10):1700009.
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