[1] DATE Y,KOJIMA M,HOSODA H,et al.Ghrelin,a novel growth hormone-releasing acylated peptide,is synthesized in a distinct endocrine cell type in the gastrointestinal tracts of rats and humans[J].Endocrinology,2000,141(11):4255-4261.

[2] KHATIB N,GAIDHANE S,GAIDHANE A M,et al.Ghrelin:ghrelin as a regulatory peptide in growth hormone secretion[J].Journal of Clinical and Diagnostic Research:JCDR,2014,8(8):MC13-MC17.
[3] TAKIGUCHI S,MURAKAMI K,YANAGIMOTO Y,et al.Clinical application of ghrelin in the field of surgery[J].Surgery Today,2015,45(7):801-807.

[4] FAIM F,PASSAGLIA P,BATALHAO M,et al.Role of ghrelin on growth hormone/insulin-like growth factor-1 axis during endotoxemia[J].Growth Hormone & IGF Research,2019,48-49:36-44.
[5] HUANG H H,CHEN C Y.Alpha-melanocyte stimulating hormone in ghrelin-elicited feeding and gut motility[J].Journal of the Chinese Medical Association,2019,82(2):87-91.

[6] THEANDER-CARRILLO C,WIEDMER P,CETTOUR-ROSE P,et al.Ghrelin action in the brain controls adipocyte metabolism[J].Journal of Clinical Investigation,2006,116(7):1983-1993.

[7] LV Y,LIANG T T,WANG G X,et al.Ghrelin,a gastrointestinal hormone,regulates energy balance and lipid metabolism[J].Bioscience Reports,2018,38(5):BSR20181061.
[8] 马兴彬,许伟华.Ghrelin与胃肠疾病关系的研究进展[J].世界华人消化杂志,2013,21(3):239-243. MA X B,XU W H.Relationship between Ghrelin and gastrointestinal diseases[J].World Chinese Journal of Digestology,2013,21(3):239-243.(in Chinese)
[9] LI Z R,MULHOLLAND M,ZHANG W Z.Ghrelin O-acyltransferase (GOAT) and energy metabolism[J].Science China Life Sciences,2016,59(3):281-291.

[10] AL MASSADI O,NOGUEIRAS R,DIEGUEZ C,et al.Ghrelin and food reward[J].Neuropharmacology,2019,148:131-138.
[11] SEIM I,LUBIK A A,LEHMAN M L,et al.Cloning of a novel insulin-regulated ghrelin transcript in prostate cancer[J].Journal of Molecular Endocrinology,2013,50(2):179-191.
[12] SEIM I,COLLET C,HERINGTON A C,et al.Revised genomic structure of the human ghrelin gene and identification of novel exons,alternative splice variants and natural antisense transcripts[J].BMC Genomics,2007,8:298.
[13] KOJIMA M,KANGAWA K.Ghrelin:from gene to physiological function[J].Results and Problems in Cell Differentiation,2010,50:185-205.
[14] KANAMOTO N,AKAMIZU T,TAGAMI T,et al.Genomic structure and characterization of the 5'-flanking region of the human ghrelin gene[J].Endocrinology,2004,145(9):4144-4153.

[15] SATO T,NAKAMURA Y,SHⅡMURA Y,et al.Structure,regulation and function of ghrelin[J].The Journal of Biochemistry,2012,151(2):119-128.

[16] HILL J T,CHAO C S,ANDERSON K R,et al.Nkx2.2 activates the ghrelin promoter in pancreatic islet cells[J].Molecular Endocrinology,2010,24(2):381-390.

[17] SHⅡMURA Y,OHGUSU H,SATO T,et al.Regulation of the human ghrelin promoter activity by transcription factors,NF-κB and Nkx2.2[J].International Journal of Endocrinology,2015,2015:580908.
[18] ZHU X R,CAO Y,VOODG K,et al.On the processing of proghrelin to ghrelin[J].Journal of Biological Chemistry,2006,281(50):38867-38870.

[19] VERHULST P J,DEPOORTERE I.Ghrelin's second life:from appetite stimulator to glucose regulator[J].World Journal of Gastroenterology,2012,18(25):3183-3195.
[20] DEPOORTERE I.GI functions of GPR39:novel biology[J].Current Opinion in Pharmacology,2012,12(6):647-652.

[21] MURTUZA M I,ISOKAWA M.Endogenous ghrelin-O-acyltransferase (GOAT) acylates local ghrelin in the hippocampus[J].Journal of Neurochemistry,2018,144(1):58-67.

[22] LEMARIÉ F,BEAUCHAMP E,LEGRAND P,et al.Revisiting the metabolism and physiological functions of caprylic acid (C8:0) with special focus on ghrelin octanoylation[J].Biochimie,2015,120:40-48.
[23] YANG J,BROWN M S,LIANG G S,et al.Identification of the acyltransferase that octanoylates ghrelin,an appetite-stimulating peptide hormone[J].Cell,2008,132(3):387-396.

[24] LIM C T,KOLA B,GROSSMAN A,et al.The expression of ghrelin O-acyltransferase (GOAT) in human tissues[J].Endocrine Journal,2011,58(8):707-710.

[25] YONEYAMA-HIROZANE M,DEGUCHI K,HIRAKAWA T,et al.Identification and characterization of a new series of ghrelin O-acyl transferase inhibitors[J].SLAS Discovery:Advancing Life Sciences R & D,2017,23(2):154-163.
[26] DELPORTE C.Structure and physiological actions of ghrelin[J].Scientifica,2013,2013:518909.
[27] DARLING J E,ZHAO F,LOFTUS R J,et al.Structure-activity analysis of human ghrelin o-acyltransferase reveals chemical determinants of ghrelin selectivity and acyl group recognition[J].Biochemistry,2015,54(4):11-19.
[28] HOUGLAND J L.Ghrelin octanoylation by ghrelin
O-acyltransferase:unique protein biochemistry underlying metabolic signaling[J].Biochemical Society Transactions,2019,47(1):169-178.

[29] MOHAN H,GASNER M,RAMESH N,et al.Ghrelin,ghrelin-O-acyl transferase,nucleobindin-2/nesfatin-1 and prohormone convertases in the pancreatic islets of Sprague Dawley rats during development[J].Journal of Molecular Histology,2016,47(3):325-336.

[30] WALIA P,ASADI A,KIEFFER T J,et al.Ontogeny of ghrelin,obestatin,preproghrelin,and prohormone convertases in rat pancreas and stomach[J].Pediatric Research,2008,65(1):39-44.
[31] SEIM I,JEFFERY P L,DE AMORIM L,et al.Ghrelin O-acyltransferase (GOAT) is expressed in prostate cancer tissues and cell lines and expression is differentially regulated
in vitro by ghrelin[J].Reproductive Biology and Endocrinology,2013,11:70.
[32] NAKAJIMA K,MAEDA N,OISO S,et al.Decreased plasma octanoylated ghrelin levels in mice by oleanolic acid[J].Journal of Oleo Science,2019,68(1):103-109.

[33] PATTERSON M,MURPHY K G,LE ROUX C W,et al.Characterization of ghrelin-like immunoreactivity in human plasma[J].Journal of Clinical Endocrinology and Metabolism,2005,90(4):2205-2211.

[34] HA Z Y,MATHEW S,YEONG K Y.Butyrylcholinesterase:a multifaceted pharmacological target and tool[J].Current Protein & Peptide Science,2020,21(1):99-109.

[35] CLEVERDON E R,MCGOVERN-GOOCH K R,HOUGLAND J L.The octanoylated energy regulating hormone ghrelin:an expanded view of ghrelin's biological interactions and avenues for controlling ghrelin signaling[J].Molecular Membrane Biology,2017,33(6/7/8):111-124.
[36] BRIMIJOIN S,CHEN V P,PANG Y P,et al.Physiological roles for butyrylcholinesterase:a BChE-ghrelin axis[J].Chemico-Biological Interactions,2016,259:271-275.
[37] CHEN V P,GAO Y,GENG L Y,et al.Plasma butyrylcholinesterase regulates ghrelin to control aggression[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(12):2251-2256.
[38] CHEN N P,GAO Y,GENG L,et al.Butyrylcholinesterase regulates central ghrelin signaling and has an impact on food intake and glucose homeostasis[J].International Journal of Obesity,2017,41(9):1413-1419.

[39] CUMMINGS D E,PURNELL J Q,FRAYO R S,et al.A preprandial rise in plasma ghrelin levels suggests a role in meal initiation in humans[J].Diabetes,2001,50(8):1714-1719.

[40] TONG J,PRIGEON R L,DAVIS H W,et al.Ghrelin suppresses glucose-stimulated insulin secretion and deteriorates glucose tolerance in healthy humans[J].Diabetes,2010,59(9):2145-2151.

[41] SAKATA I,PARK W M,WALKER A K,et al.Glucose-mediated control of ghrelin release from primary cultures of gastric mucosal cells[J].American Journal of Physiology:Endocrinology and Metabolism,2012,302(10):E1300-E1310.
[42] OYA M,KITAGUCHI T,HARADA K,et al.Low glucose-induced ghrelin secretion is mediated by an ATP-sensitive potassium channel[J].Journal of Endocrinology,2015,226(1):25-34.

[43] DEZAKI K.Ghrelin function in insulin release and glucose metabolism[J].Endocrine development,2013,25:135-43.
[44] ALAMRI B N,SHIN K,CHAPPE V,et al.The role of ghrelin in the regulation of glucose homeostasis[J].Hormone Molecular Biology and Clinical Investigation,2016,26(1):3-11.
[45] AUCLAIR N,PATEY N,MELBOUCI L,et al.Acylated ghrelin and the regulation of lipid metabolism in the intestine[J].Scientific Reports,2019,9:17975.
[46] NISHI Y,MIFUNE H,KOJIMA M.Ghrelin acylation by ingestion of medium-chain fatty acids[J].Methods in Enzymology,2012,514:303-315.
[47] LU X P,ZHAO X L,FENG J Y,et al.Postprandial inhibition of gastric ghrelin secretion by long-chain fatty acid through GPR120 in isolated gastric ghrelin cells and mice[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,2012,303(3):G367-G376.
[48] BANDO M,IWAKURA H,KOYAMA H,et al.High incorporation of long-chain fatty acids contributes to the efficient production of acylated ghrelin in ghrelin-producing cells[J].FEBS Letters,2016,590(7):992-1001.

[49] GONG Z,YOSHIMURA M,AIZAWA S,et al.G protein-coupled receptor 120 signaling regulates ghrelin secretion
in vivo and
in vitro[J].American Journal of Physiology Endocrinology and Metabolism,2014,306(1):E28-E35.
[50] SUGINO T,KAWAKITA Y,FUKUMORI R,et al.Effects of glucose and amino acids on ghrelin secretion in sheep[J].Animal Science Journal,2010,81(2):199-204.

[51] FUKUMORI R,YOKOTANI A,SUGINO T,et al.Effects of amino acids infused into the vein on ghrelin-induced GH,insulin and glucagon secretion in lactating cows[J].Animal Science Journal,2011,82(2):267-273.

[52] ELSABAGH M,ISHIKAKE M,SAKAMOTO Y,et al.Postruminal supply of amino acids enhances ghrelin secretion and lipid metabolism in feed-deprived sheep[J].Animal Science Journal,2018,89(12):1663-1672.

[53] VANCLEEF L,VAN DEN BROECK T,THIJS T,et al.Chemosensory signalling pathways involved in sensing of amino acids by the ghrelin cell[J].Scientific Reports,2015,5:15725.
[54] ZHANG B K,GUO Y M.The growth-promoting effect of tetrabasic zinc chloride is associated with elevated concentration of growth hormone and Ghrelin[J].Asian-Australasian Journal of Animal Sciences,2008,21(10):1473-1478.

[55] YIN J D,LI X L,LI D F,et al.Dietary supplementation with zinc oxide stimulates ghrelin secretion from the stomach of young pigs[J].Journal of Nutritional Biochemistry,2009,20(10):783-790.

[56] QIAO J W,ZHAO H S,ZHANG Y,et al.GPR39 is region-specifically expressed in mouse oviduct correlating with the Zn
2+ distribution[J].Theriogenology,2016,88:98-105.
[57] POPOVICS P,STEWART A J.GPR39:a Zn
2+-activated G protein-coupled receptor that regulates pancreatic,gastrointestinal and neuronal functions[J].Cellular and Molecular Life Sciences,2011,68(1):85-95.

[58] YANG W Y,WANG J G,ZHU X Y,et al.High lever dietary copper promote ghrelin gene expression in the fundic gland of growing pigs[J].Biological Trace Element Research,2012,150(1/2/3):154-157.
[59] 黄元龙.日粮铜水平对绵羊生长激素释放调节多肽的影响[D].硕士学位论文.长春:吉林农业大学,2018. HUANG Y L.Effect of dietary copper level on growth hormone releasing regulatory polypeptide in sheep[D].Master's Thesis.Changchun:Jilin Agricultural University,2018.(in Chinese)
[60] LUNG S,LI H H,BONDY S C,et al.Low concentrations of copper in drinking water increase AP-1 binding in the brain[J].Toxicology and Industrial Health,2013,31(12):1178-1184.
[61] MATTIE M D,MCELWEE M K,FREEDMAN J H.Mechanism of copper-activated transcription:activation of AP-1,and the JNK/SAPK and p38 signal transduction pathways[J].Journal of Molecular Biology,2008,383(5):1008-1018.

[62] REYNAUD Y,FAKHRY J,FOTHERGILL L,et al.The chemical coding of 5-hydroxytryptamine containing enteroendocrine cells in the mouse gastrointestinal tract[J].Cell and Tissue Research,2016,364(3):489-497.

[63] HUNNE B,STEBBING M J,MCQUADE R M,et al.Distributions and relationships of chemically defined enteroendocrine cells in the rat gastric mucosa[J].Cell and Tissue Research,2019,378(1):33-48.

[64] FUKUHARA S,SUZUKI H,MASAOKA T,et al.Enhanced ghrelin secretion in rats with cysteamine-induced duodenal ulcers[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2005,289(1):G138-G145.
[65] SILVA A P,BETHMANN K,RAULF F,et al.Regulation of ghrelin secretion by somatostatin analogs in rats[J].European Journal of Endocrinology,2005,152(6):887-894.

[66] STENGEL A,GOEBEL M,WANG L X,et al.Abdominal surgery inhibits circulating Acyl ghrelin and ghrelin-O-acyltransferase levels in rats:role of the somatostatin receptor 2[J].Gastroenterology,2011,140(5 suppl. 1):S-808.
[67] 杜改梅,吴结革,胡志华,等.断奶前后小鼠胃
GOAT基因表达及其与胃酸分泌、胃泌素和生长抑素的关系[J].江苏农业学报,2016,32(3):626-630. DU G M,WU J G,HU Z H.Expression of gastric GOAT mRNA and the relationship with gastric acid secretion,gastric gastrin and SS in pre- and post-weaning mice[J].Jiangsu Journal of Agricultural Sciences,2016,32(3):626-630.(in Chinese)
[68] GAHETE M D,CÓRDOBA-CHACÓN J,SALVATORI R,et al.Metabolic regulation of ghrelin O-acyl transferase (
GOAT) expression in the mouse hypothalamus,pituitary,and stomach[J].Molecular and Cellular Endocrinology,2010,317(1/2):154-160.
[69] KLOK M D,JAKOBSDOTTIR S,DRENT M L.The role of leptin and ghrelin in the regulation of food intake and body weight in humans:a review[J].Obesity Reviews,2007,8(1):21-34.

[70] KIYICI S,BASARAN N F,CAVUN S,et al.Central injection of CDP-choline suppresses serum ghrelin levels while increasing serum leptin levels in rats[J].European Journal of Pharmacology,2015,764:264-270.
[71] ZHAO Z,SAKATA I,OKUBO Y,et al.Gastric leptin,but not estrogen and somatostatin,contributes to the elevation of ghrelin mRNA expression level in fasted rats[J].Journal of Endocrinology,2008,196(3):529-538.
[72] PINKNEY J.The role of ghrelin in metabolic regulation[J].Current Opinion in Clinical Nutrition and Metabolic Care,2014,17(6):497-502.

[73] NOUR H A,EL SAWAF A L,ELEWA S M,et al.Strength and independence of associations between ghrelin,leptin,adiponectin and insulin in stimulating basic functions to energy metabolism[J].Alexandria Journal of Medicine,2014,50(1):49-59.

[74] 孙艺璇.脑肠肽与胰岛素分泌[J].同济大学学报(医学版),2014,35(3):133-136. SUN Y X.Braingutpeptides in regulation of insulin secretion[J].Journal of Tongji University(Medical Science),2014,35(3):133-136.(in Chinese)
[75] VAN NAME M,GIANNINI C,SANTORO N,et al.Blunted suppression of acyl-ghrelin in response to fructose ingestion in obese adolescents:the role of insulin resistance[J].Obesity,2015,23(3):653-661.

[76] BLIJDORP K,VAN DER LELY A J,VAN DEN HEUVEL-EIBRINK M M,et al.Desacyl ghrelin is influenced by changes in insulin concentration during an insulin tolerance test[J].Growth Hormone & IGF Research,2013,23(5):193-195.

[77] SONG X X,JIAO H C,ZHAO J P,et al.Dexamethasone and insulin stimulate ghrelin secretion of broilers in a different way[J].General and Comparative Endocrinology,2018,268:14-21.
[78] UENO M,CARVALHEIRA J B C,OLIVEIRA R L G S,et al.Expression of concern:circulating ghrelin concentrations are lowered by intracerebroventricular insulin[J].Diabetologia,2017,doi:10.1007/s00125-017-4357-5.
[79] PAGE L C,GASTALDELLI A,GRAY S M,et al.Interaction of GLP-1 and Ghrelin on Glucose Tolerance in Healthy Humans[J].Diabetes,2018,67(10):1976-1985.

[80] HAGEMANN D,HOLST J J,GETHMANN A,et al.Glucagon-like peptide 1(GLP-1) suppresses ghrelin levels in humans via increased insulin secretion[J].Regulatory Peptides,2007,143(1/2/3):64-68.
[81] RONVEAUX C C,TOMÉ D,RAYBOULD H E.Glucagon-like peptide 1 interacts with ghrelin and leptin to regulate glucose metabolism and food intake through vagal afferent neuron signaling[J].The Journal of Nutrition,2015,145(4):672-680.

[82] HONG X S,ZHANG H,LIANG H B,et al.Exendin-4 decreases ghrelin levels through mTOR signaling[J].Molecular and Cellular Endocrinology,2016,437:201-212.
[83] HOSODA H,KANGAWA K.The autonomic nervous system regulates gastric ghrelin secretion in rats[J].Regulatory Peptides,2008,146(1/2/3):12-18.
[84] MUNDINGER T O,CUMMINGS D E,TABORSKY G J,Jr.Direct stimulation of ghrelin secretion by sympathetic nerves[J].Endocrinology,2006,147(6):2893-2901.

[85] ZHAO T J,SAKATA I,LI R L,et al.Ghrelin secretion stimulated by β1-adrenergic receptors in cultured ghrelinoma cells and in fasted mice[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(36):15868-15873.

[86] MANI B K,CHUANG J C,KJALARSDOTTIR L,et al.Role of calcium and EPAC in norepinephrine-induced ghrelin secretion[J].Endocrinology,2014,155(1):98-107.