禽营养与饲料 POULTRY NUTRITION AND FEED

饲养密度和硫辛酸对肉鸡血清指标、行为及骨骼发育的影响

  • 李文嘉 ,
  • 徐彬 ,
  • 马慧慧 ,
  • 魏凤仙 ,
  • 王琳燚 ,
  • 付趁 ,
  • 王改利 ,
  • 孙全友 ,
  • 李绍钰
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  • 河南省农业科学院畜牧兽医研究所, 郑州 450002
李文嘉(1987-),男,湖北襄阳人,助理研究员,博士,从事动物营养与饲料科学研究。E-mail:liwenjia_2008@163.com

收稿日期: 2020-06-18

  网络出版日期: 2021-01-18

基金资助

国家重点研发计划项目(2016YFD0500509);河南省农业科学院优秀青年科技基金项目(2020YQ27);现代农业产业技术体系建设专项(CARS-41);河南省农业科学院自主创新项目(2020ZC39)

Effects of Stocking Density and Alpha-Lipoic Acid on Serum Indexes, Behavior and Bone Development of Broilers

  • LI Wenjia ,
  • XU Bin ,
  • MA Huihui ,
  • WEI Fengxian ,
  • WANG Linyi ,
  • FU Chen ,
  • WANG Gaili ,
  • SUN Quanyou ,
  • LI Shaoyu
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  • Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

Received date: 2020-06-18

  Online published: 2021-01-18

摘要

本试验旨在研究高饲养密度条件下饲粮中添加硫辛酸(LA)对肉鸡血清指标、行为及骨骼发育的影响。试验选取健康状况良好、体重相近的1 530只21日龄爱拔益加(AA)肉鸡,随机分成3组,即常规饲养密度组(ND组,15只/m2,饲喂基础饲粮)、高饲养密度组(HD组,18只/m2,饲喂基础饲粮)和高饲养密度+LA组(HD+LA组,18只/m2,在基础饲粮在添加300 mg/kg LA),每组6个重复。试验期为3周。结果表明:1)与ND组相比,HD组肉鸡血清钙含量和碱性磷酸酶(ALP)活性显著下降(P<0.05),血清皮质酮(CORT)含量以及还原型辅酶Ⅱ氧化酶(NOX)和髓过氧化物酶(MPO)活性显著上升(P<0.05)。2)与ND组相比,HD组肉鸡行走、梳翅、采食频次与时间占比显著降低(P<0.05),而饮水频次以及饮水和趴卧时间占比显著升高(P<0.05)。3)与ND组相比,高饲养密度显著降低肉鸡胫骨与股骨直径、重量、密度、强度以及钙、磷、灰分和矿物盐含量(P<0.05)。4)与HD组相比,HD+LA组肉鸡血清钙含量和ALP活性显著升高(P<0.05),血清CORT含量以及NOX和MPO活性显著下降(P<0.05);采食频次和时间占比显著增加(P<0.05),饮水频次和趴卧时间占比显著降低(P<0.05);胫骨质量以及钙、磷含量与股骨密度、强度和钙含量显著上升(P<0.05)。综上所述,高饲养密度致使肉鸡发生氧化应激,改变了肉鸡行为,并使肉鸡骨骼质量下降,而饲粮中添加LA能在一定程度上缓解高饲养密度对肉鸡造成的损伤。

本文引用格式

李文嘉 , 徐彬 , 马慧慧 , 魏凤仙 , 王琳燚 , 付趁 , 王改利 , 孙全友 , 李绍钰 . 饲养密度和硫辛酸对肉鸡血清指标、行为及骨骼发育的影响[J]. 动物营养学报, 2021 , 33(1) : 236 -247 . DOI: 10.3969/j.issn.1006-267x.2021.01.025

Abstract

This experiment was conducted to investigate the effects of dietary supplemented with alpha-lipoic acid (LA) on the serum indexes, behavior and bone development of broilers under high stocking density condition. In the experiment, a total of 1 530 Arbor Acres AA ()broilers at the age of 21 days with good health and similar body weight were selected and randomly divided into three groups, they were normal stocking density group (ND group, 15 birds/m2, basal diet), high stocking density group (18 bird/m2, basal diet) and high stocking density+LA group (HD+LA group, 18 birds/m2, basal diet+300 mg/kg LA), and each group had 6 replicates. The experimental period was 3 weeks. The results showed as follows: 1) the serum calcium content and alkaline phosphatase (ALP) activity of broilers were significantly decreased (P<0.05), and the corticosterone (CORT) content, NADPH-oxidase (NOX) and myeloperoxidase (MPO) activities were significantly increased (P<0.05) in HD group compared with the ND group. 2) Compared with the ND group, the walking, combing and feeding frequencies and time proportions of broiler's walking, feeding and combing were significantly decreased (P<0.05), and the frequency and time proportion of drinking were significantly increased in HD group (P<0.05). 3) High stocking density significantly lowered the diameter, weight, density, strength and the contents of calcium, phosphorus, ash and mineral of tibia and thighbone compared with the ND group (P<0.05). 4) Compared with the HD group, the serum calcium content and ALP activity of broilers were significantly increased (P<0.05), and the content of CORT and the activities of NOX and MPO were significantly decreased in the HD+LA group (P<0.05); the feeding frequency and time proportion of broilers were significantly increased (P<0.05), and the drinking frequency and lying time proportion were significantly decreased in the HD+LA group (P<0.05); the tibia weight and Ca and P content and the thighbone density, strength and calcium content of broilers were significantly increased in the HD+LA group (P<0.05). In conclusion, high stocking density leads to oxidative stress, modifies the behavior and lowers the bone quality of broilers. Dietary addition of alpha-lipoic acid can alleviate the damage to broilers caused by high stocking density to a certain extent.

参考文献

[1] DOZIER Ⅲ W A,THAXTON J P,BRANTON S L,et al.Stocking density effects on growth performance and processing yields of heavy broilers[J].Poultry Science,2005,84(8):1332-1338.  
[2] DOZIER Ⅲ W A,THAXTON J P,PURSWELL J L,et al.Stocking density effects on male broilers grown to 1.8 kilograms of body weight[J].Poultry Science,2006,85(2):344-351.  
[3] ERICSSON M,FALLAHSHAROUDI A,BERGQUIST J,et al.Domestication effects on behavioural and hormonal responses to acute stress in chickens[J].Physiology & Behavior,2014,133:161-169.
[4] LI W J,WEI F X,XU B,et al.Effect of stocking density and alpha-lipoic acid on the growth performance,physiological and oxidative stress and immune response of broilers[J].Asian-Australasian Journal of Animal Sciences,2019,32(12):1914-1922.  
[5] 田允波.减缓肉鸡热应激的措施[J].中国家禽,2000,22(6):33-34. TIAN Y B.Measures to reduce the heat stress of broilers[J].China Poultry,2000,22(6):33-34.(in Chinese)
[6] 刘文波.饲养密度和季节对肉仔鸡猝死综合症发生的影响[J].中国家禽,2002,24(5):17. LIU W B.Effects of feeding density and season on sudden death syndrome in broilers[J].China Poultry,2002,24(5):17.(in Chinese)
[7] VAN REENEN C G,O'CONNELL N E,VAN DER WERF J T N,et al.Responses of calves to acute stress:individual consistency and relations between behavioral and physiological measures[J].Physiology & Behavior,2005,85(5):557-570.  
[8] PACKER L,WITT E H,TRITSCHLER H J.Alpha-lipoic acid as a biological antioxidant[J].Free Radical Biology and Medicine,1995,19(2):227-250.  
[9] BIEWENGA G P,HAENEN G R M M,BAST A.The pharmacology of the antioxidant lipoic acid[J].General Pharmacology:The Vascular System,1997,29(3):315-331.  
[10] BILSKA A,W?ODEK L.Lipoic acid-the drug of the future[J].Pharmacological Reports,2005,57(5):570-577.
[11] EKLUND B,JENSEN P.Domestication effects on behavioural synchronization and individual distances in chickens (Gallus gallus)[J].Behavioural Processes,2010,86(2):250-256.
[12] 厉秀梅,高杰,冯京海,等.相对湿度对间歇性偏热环境下肉鸡生长性能、行为及空肠形态结构的影响[J].动物营养学报,2017,29(10):3506-3513. LI X M,GAO J,FENG J H,et al.Effects of relative humidity at intermittent moderate temperature on growth performance,behavior and jejunum morphological structure of broilers[J].Chinese Journal of Animal Nutrition,2017,29(10):3506-3513.(in Chinese)
[13] 朱晓彤,刘连燕,王丽娜,等.不同降温措施和不同日龄放养模式对黄羽肉鸡行为的影响[J].家畜生态学报,2014,35(6):31-37. ZHU X D,LIU L Y,WANG L N,et al.Effects of different cooling methods and outdoor stocking age on the behaviors of yellow feather broilers[J].Journal of Domestic Animal Ecology,2014,35(6):31-37.(in Chinese)
[14] DAWKINS M S,DONNELLY C A,JONES T A.Chicken welfare is influenced more by housing conditions than by stocking density[J].Nature,2004,427(6972):342-344.  
[15] 孙庆雨,杨海明,肖宇航,等.不同饲养密度对鹌鹑生产性能、羽毛评分及血清免疫和生化指标的影响[J].动物营养学报,2020,32(5):2192-2197. SUN Q Y,YANG H M,XIAO Y H,et al.Effects of different stocking densities on performance,feather score and serum immune and biochemical indicators of quail[J].Chinese Journal of Animal Nutrition,2020,32(5):2192-2197.(in Chinese)
[16] ÖZBEY O,ESEN F.The effects of breeding systems and stocking density on some blood parameters of rock partridges (Alectoris graeca)[J].Poultry Science,2007,86(2):420-422.  
[17] 姜金庆,郭泽坤,杨雪峰,等.电解质平衡值对热应激时肉鸡生产性能和血液生化指标的影响[J].安徽农业科学,2006,34(10):2144-2146. JIANG J Q,GUO Z K,YANG X F,et al.Effect of dietary electrolyte balance on growth performance and biochemical indexes of broilers in heat stress[J].Journal of Anhui Agricultural Sciences,2006,34(10):2144-2146.(in Chinese)
[18] VAN GOOR A,ASHWELL C M,PERSIA M E,et al.Quantitative trait loci identified for blood chemistry components of an advanced intercross line of chickens under heat stress[J].BMC Genomics,2016,17:287.
[19] 李香.急性偏热环境对肉鸡行为、生理生化指标及avUCP mRNA表达影响[D].硕士学位论文.邯郸:河北工程大学,2017. LI X.Effect of acute moderate temperatures on behavior,physiology and biochemical,avUCP mRNA expression of broilers[D].Master's Thesis.Handan:Hebei University of Engineering,2017.(in Chinese)
[20] HOWLETT C R,CAVÉ J,WILLIAMSON M,et al.Mineralization in in vitro cultures of rabbit marrow stromal cell[J].Clinical Orthopaedics and Related Research,1986(213):251-263.
[21] 冯永淼.过量维生素A对肉仔鸡骨骼钙磷代谢影响及机理的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2007. FENG Y M.Effects of excessive vitamin a on calcium and phosphorous metabolism in broilers and underlying mechanisms[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University,2007.(in Chinese)
[22] 袁建敏.肉禽饲养密度应激及营养调控研究进展[J].中国家禽,2017,39(17):1-5. YUAN J M.Research progress on density stress and nutrition regulation of meat poultry[J].China Poultry,2017,39(17):1-5.(in Chinese)
[23] 秦鑫,卢营杰,苗志强,等.饲养方式和密度对爱拔益加肉鸡生产性能、肉品质及应激的影响[J].中国农业大学学报,2018,23(12):66-74. QING X,LU Y J,MIAO Z Q,et al.Effects of rearing system and stocking density on the growth performance,meat quality and stress response of Arbor Acres broilers[J].Journal of China Agricultural University,2018,23(12):66-74.(in Chinese)
[24] KOTHARI N,KESHARI R S,BOGRA J,et al.Increased myeloperoxidase enzyme activity in plasma is an indicator of inflammation and onset of sepsis[J].Journal of Critical Care,2011,26(4):431.e1-435.e7.
[25] SU H S,NAHRENDORF M,PANIZZI P,et al.Vasculitis:molecular imaging by targeting the inflammatory enzyme myeloperoxidase[J].Radiology,2012,262(1):181-190.  
[26] 郭志有.α-硫辛酸对肉鸡抗氧化的影响及其作用机制研究[D].博士学位论文.南京:南京农业大学,2014. GUO Z Y.Study on the anti-oxidant capacity of alpha-lipoic acid and the antioxidant mechanism in broilers[D].Ph.D.Thesis.Nanjing:Nanjing Agricultural University,2014.(in Chinese)
[27] NEWBERR R C.Environmental enrichment:increasing the biological relevance of captive environments[J].Applied Animal Behaviour Science,1995,44(2/3/4):229-243.
[28] 范石军,韩友文,李德发,等.热应激对产蛋鸡自身及其后代机体组织的过氧化损伤以及抗氧化微营养素的调控效应[J].中国兽医学报,2001,21(2):195-199. FAN S J,HAN Y W,LI D F,et al.Regulative effect of adding anti-oxidative micro-nutrients to diets on the peroxidative damages of layers and their offsprings under acute ambient temperature[J].Chinese Journal of Veterinary Science,2001,21(2):195-199.(in Chinese)
[29] IMAEDA N.Influence of the stocking density and rearing season on incidence of sudden death syndrome in broiler chickens[J].Poultry Science,2000,79(2):201-204.  
[30] SHEPHERDSON D J.Tracing the path of environmental enrichment in zoos[M]//SHEPHERDSON D J,MELLEN J D,HUTCHINS M.Environmental enrichment for captive animals.London,UK:Smithsonian Institution Press,1998:1-12.
[31] 张增玉,顾宪红,赵恒寿,等.现代肉鸡生产中的福利问题[J].家畜生态学报,2006,27(2):5-12. ZHANG Z Y,GU X H,ZHAO H S,et al.Welfare of broiler chicken in modern poultry industry[J].Journal of Domestic Animal Ecology,2006,27(2):5-12.(in Chinese)
[32] BUIJS S,VAN POUCKE E,VAN DONGEN S,et al.The influence of stocking density on broiler chicken bone quality and fluctuating asymmetry[J].Poultry Science,2012,91(8):1759-1767.  
[33] 饶盛达.不同维生素组合和饲养密度对肉鸡生产性能、健康和肉品质的影响研究[D].硕士学位论文.雅安:四川农业大学,2015. RAO S D.Effects of different vitamin premixes and stocking density on performance,health,and meat quality of broilers[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2015.(in Chinese)
[34] VENTURA B A,SIEWERDT F,ESTEVEZ I.Access to barrier perches improves behavior repertoire in broilers[J].PLoS One,2012,7(1):e29826,doi:10.1371/journal.pone.0029826.
[35] SIMITZIS P E,KALOGERAKI E,GOLIOMYTIS M,et al.Impact of stocking density on broiler growth performance,meat characteristics,behavioural components and indicators of physiological and oxidative stress[J].British Poultry Science,2012,53(6):721-730.  
[36] MACK L A,FELVER-GANT J N,DENNIS R L,et al.Genetic variations alter production and behavioral responses following heat stress in 2 strains of laying hens[J].Poultry Science,2013,92(2):285-294.  
[37] 胡春红.环境温度对肉鸡生理和行为调节的影响[D].硕士学位论文.北京:中国农业科学院,2015. HU C H.The influence of environment temperature on physiological and behavioral regulation in broilers[J].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2015.(in Chinese)
[38] 白兆鹏.硫辛酸对热应激下产蛋鸡生产性能及抗氧化能力的研究[D].硕士学位论文.哈尔滨:东北农业大学,2004. BAI Z P.Study of lipoic acid on layers performance and antioxidant function under heat-stress[D].Master's Thesis.Harbin:Northeast Agricultural University,2004.(in Chinese)
[39] 倪可德.动物微量元素营养(一)[J].粮食与饲料工业,1996(2):28-32. NI K D.Animal micronutrient nutrition (1)[J].Cereal and Feed Industry,1996(2):28-32.(in Chinese)
[40] KESTIN S C,KNOWLES T G,TINCH A E,et al.Prevalence of leg weakness in broiler chickens and its relationship with genotype[J].Veterinary Record,1992,131(9):190-194.  
[41] SANOTRA G S,LAWSON L G,VESTERGAARD K S,et al.Influence of stocking density on tonic immobility,lameness,and tibial dyschondroplasia in broilers[J].Journal of Applied Animal Welfare Science,2001,4(1):71-87.  
[42] SUN Z W,YAN L,G Y Y,et al.Increasing dietary vitamin D3 improves the walking ability and welfare status of broiler chickens reared at high stocking densities[J].Poultry Science,2013,92(12):3071-3079.  
[43] 张璐爽,张亚茹,刘洋,等.网上饲养密度对北京鸭免疫功能和氧化应激水平的影响[J].中国家禽,2015,37(12):31-34. ZHANG L S,ZHANG Y G,LIU Y,et al.Effects of stocking density on immune function and oxidative stress level of pekin ducks reared on plastic wire-floor[J].China Poultry,2015,37(12):31-34.(in Chinese)
[44] HALL A L.The effect of stocking density on the welfare and behaviour of broiler chickens reared commercially[J].Animal Welfare,2001,10(1):23-40.
[45] VESTERGAARD S,SANOTRA G S.Relationships between leg disorders and changes in the behaviour of broiler chickens[J].Veterinary Record,1999,144(8):205-209.  
[46] ESTEVEZ I,NEWBERRY R C,DE RAYNA L A.Broiler chickens:a tolerant social system?[J].Etología,1997,5(2):19-29.  
[47] NEWBERRY R C,HUNT J R,GARDINER E E.Influence of light intensity on behavior and performance of broiler chickens[J].Poultry Science,1988,67(7):1020-1025.  
[48] ŠKRBI? Z,PAVLOVSKI Z,LUKI? M.Stocking density:factor of production performance,quality and broiler welfare[J].Biotechnology in Animal Husbandry,2009,25(5/6):359-372.
[49] MODY N,PARHAMI F,SARAFIAN T A,et al.Oxidative stress modulates osteoblastic differentiation of vascular and bone cells[J].Free Radical Biology and Medicine,2001,31(4):509-519
[50] BLAIR H C,SCHLESINGER P H,ROSS F P,et al.Recent advances toward understanding osteoclast physiology[J].Clinical Orthopaedics and Related Research,1993(294):7-22.
[51] LEAN J M,JAGGER C J,KIRSTEIN B,et al.Hydrogen peroxide is essential for estrogen-deficiency bone loss and osteoclast formation[J].Endocrinology,2005,146(2):728-735.  
[52] 白水莉.饲养密度和环境富集材料对肉鸡福利状况、生产性能和肉品质的影响[D].硕士学位论文.扬州:扬州大学,2009. BAI S L.Effect of Stocking density and environmental enrichment materials on the welfare level,production performance and meat quality in broiler[D].Master's Thesis.Yangzhou:Yangzhou University,2009.(in Chinese)
[53] 蔡吓明.氧化应激在糖尿病鼠高血糖所致骨质疏松发病中的作用[D].硕士学位论文.福州:福建医科大学,2010. CAI X M.The effect of oxidative stress in causing the osteoporosis of the mouses with diabetes[D].Master's Thesis.Fuzhou:Fujian Medical University,2010.(in Chinese)
[54] 朱琳玲.骨胶原肽对高脂膳食小鼠抗氧化能力及皮肤和骨骼胶原代谢的影响[D].硕士学位论文.无锡:江南大学,2012. ZHU L L.The effects of ossein peptide on antioxidant capacity and collagen metabolism of skin and bone in high-fat diet km mice[D].Master's Thesis.Wuxi:Jiangnan University,2012.(in Chinese)
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