动物营养学报 >

2021 , Vol. 33 >Issue 1: 297 - 307

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.030

禽营养与饲料 POULTRY NUTRITION AND FEED

橙皮苷对肉仔鸡生长性能、肌肉品质及肠道形态结构的影响

  • 杨季 ,
  • 王思博 ,
  • 鲁茗源 ,
  • 朱允慧 ,
  • 许丽
展开
  • 东北农业大学动物科学技术学院, 哈尔滨 150030
杨季(1997-),女,河北沧州人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:yangji209@163.com

收稿日期: 2020-06-27

  网络出版日期: 2021-01-18

基金资助

国家自然科学基金项目（31872377）

收起

Effects of Hesperidin on Growth Performance, Muscle Quality and Intestinal Morphology of Broilers

  • YANG Ji ,
  • WANG Sibo ,
  • LU Mingyuan ,
  • ZHU Yunhui ,
  • XU Li
Expand
  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2020-06-27

  Online published: 2021-01-18

Fold

摘要

本试验旨在研究在肉仔鸡饲粮中添加不同水平的橙皮苷（HSD）对其生长性能、肌肉品质及肠道形态结构的影响。采用单因子完全随机试验设计，选取健康的、体重相近的1日龄爱拔益加（AA）肉鸡公雏288只，随机分为4组，每组6个重复，每个重复12只。饲粮中HSD的添加水平分别为0（对照组）、50（HSD 50组）、100（HSD 100组）和150 mg/kg（HSD 150组）。试验期42 d。结果表明：1）在1~21日龄时，HSD添加组与对照组的体重（BW）、平均日采食量（ADFI）、平均日增重（ADG）和料重比（F/G）均无显著差异（P>0.05）。在22~42日龄时，各HSD添加组的BW和ADG均显著高于对照组（P<0.05），F/G显著低于对照组（P<0.05），且随着HSD添加水平的增加均呈线性和二次曲线变化（P<0.05）。在1~42日龄时，各HSD添加组与对照组的ADFI无显著差异（P>0.05），ADG显著高于对照组（P<0.05），F/G显著低于对照组（P<0.05），且ADG和F/G均与HSD的添加水平存在线性和二次曲线关系（P<0.05）。2）腿肌的剪切力随HSD添加水平的增加呈线性和二次曲线变化（P<0.05），其中HSD 50组及HSD 100组剪切力显著低于对照组（P<0.05）。与对照组相比，各HSD添加组屠宰24 h后肌肉的亮度（L*）值显著降低（P<0.05），且与HSD的添加水平存在线性和二次曲线关系（P<0.05）；腿肌的红度（a*）值与HSD添加水平存在线性和二次曲线关系（P<0.05），其中HSD 50组的a*值显著高于对照组（P<0.05）。各添加HSD组肌肉的吸附水含量显著高于对照组（P<0.01），胸肌粗脂肪含量显著低于对照组（P<0.05），且均与HSD的添加水平呈线性和二次曲线变化（P<0.05）；腿肌HSD 150组吸附水含量显著高于对照组（P<0.05）。3）胸肌中总抗氧化能力（T-AOC）随HSD添加水平的提高呈线性和二次曲线变化（P<0.05），其中HSD 50组的T-AOC显著高于对照组（P<0.05）；HSD 100组和HSD 150组的丙二醛（MDA）含量显著低于对照组（P<0.05），且随HSD添加水平的提高有降低趋势（P=0.098）。腿肌中HSD 50组和HSD 150组的MDA含量显著低于对照组（P<0.05），且随HSD添加水平的提高有降低趋势（P=0.087）。4）在21日龄时，与对照组相比，各添加HSD组空肠的隐窝深度显著降低（P<0.05），且随HSD添加水平的提高呈线性和二次曲线变化（P<0.05）。在42日龄时，空肠绒毛高度随HSD添加水平的提高呈二次曲线变化（P<0.05），其中HSD 150组的绒毛高度显著高于对照组（P<0.05）。综上所述，在肉仔鸡饲粮中添加50 mg/kg HSD可以有效改善其肉质，提高肌肉抗氧化能力并促进肠道发育，进而提高肉仔鸡的生长性能。

关键词： 橙皮苷; 肉仔鸡; 生长性能; 肉质; 抗氧化

本文引用格式

杨季 , 王思博 , 鲁茗源 , 朱允慧 , 许丽 . 橙皮苷对肉仔鸡生长性能、肌肉品质及肠道形态结构的影响[J]. 动物营养学报, 2021 , 33(1) : 297 -307 . DOI: 10.3969/j.issn.1006-267x.2021.01.030

Abstract

The purpose of this study was to investigate the effects of different supplemental levels of hesperidin (HSD) on the growth performance, muscle quality and intestinal morphology of broilers. A total of 288 healthy 1-day-old Arbor Acres (AA) male broilers were selected in a single-factor completely randomized trial and were randomly divided into 4 groups, with 6 replicates in each group. The repeat was 12 broilers. The supplement levels of HSD in the diet were 0 (control group), 50 (HSD 50 group), 100 (HSD 100 group) and 150 mg/kg (HSD 150 group). The test period was 42 d. The results showed as follows: 1) there were no significant differences in average body weight (BW), average daily feed intake (ADFI), average daily gain (ADG) and feed/gain (F/G) between the HSD supplementation groups and the control group at the age of 1 to 21 days (P>0.05). At the age of 22 to 42 days, the BW and ADG in each HSD supplementation group were significantly higher than those in the control group (P<0.05), the F/G was significantly lower than that in the control group (P<0.05), and with the increase of HSD supplement levels, the indexes shown linear and quadratic curve changes (P<0.05). At the age of 1 to 42 days, there was no significant difference in ADFI between the HSD supplementation groups and the control group (P>0.05), ADG was significantly higher than that in the control group (P<0.05), and F/G was significantly lower than that in the control group (P<0.05). ADG and F/G had linear and quadratic relationship with the increase of HSD supplement levels (P<0.05). 2) The shear force of leg muscles showed linear and quadratic changes with the increase of HSD supplement levels (P<0.05), and the shear force in HSD 50 group and HSD 100 group was significantly lower than that in the control group (P<0.05). Compared with the control group, the lightness (L*) value of muscles after 24 h slaughter in each HSD supplementation group was significantly reduced (P<0.05), and there was a linear and quadratic relationship with the increase of HSD supplement levels (P<0.05); there was a linear and quadratic relationship with the increase of HSD supplement levels (P<0.05), and the redness (a*) value in the HSD 50 group was significantly higher than that in the control group (P<0.05). The adsorbed water content of the muscle in the HSD supplementation group was significantly higher than that in the control group (P<0.05), and the crude fat content of breast muscle was significantly lower than that in the control group (P<0.05), and both showed linear and quadratic changes with the increase of HSD supplement levels (P<0.05); the adsorbed water content of the leg muscle in HSD 150 group was significantly higher than that in the control group (P<0.05). 3) Total antioxidative capacity (T-AOC) in the pectoral muscle showed linear and quadratic changes with the increase of HSD supplement levels (P<0.05), and the T-AOC in the HSD 50 group was significantly higher than that in the control group (P<0.05). The malondialdehyde (MDA) content in the HSD 100 group and HSD 150 group was significantly lower than that in the control group (P<0.05), and there was a decreasing trend as the supplement levels of HSD increased (P=0.098). The MDA content of leg muscles in the HSD 50 group and HSD 150 group was significantly lower than that in the control group (P<0.05), and there was a decreasing trend as the supplement levels of HSD increased (P=0.087). 4) At the age of 21 days, the crypt depth of the jejunum could be significantly reduced in each HSD supplementation group (P<0.05), and it changed linearly and quadraticly with the increase of HSD supplement levels (P<0.05). At the age of 42 days, the height of jejunum villi showed a quadratic curve with the increase of HSD supplement levels (P<0.05), and the villus height in HSD 150 group was significantly higher than that in control group. In summary, adding 50 mg/kg HSD to broiler diets can effectively improve meat quality, increase muscle antioxidant capacity, and promote intestinal development, thereby improving broiler's growth performance.

Key words： hesperidin; broiler; growth performance; meat quality; antioxidant

参考文献

[1] NORTH M K,ZOTTE A D,HOFFMAN L C,et al.The use of dietary flavonoids in meat production:a review[J].Animal Feed Science and Technology,2019,257:114291.
[2] UMENO A,HORIE M,MUROTOMI K,et al.Antioxidative and antidiabetic effects of natural polyphenols and isoflavones[J].Molecules,2016,21(6):708.
[3] LEE N,KWAK H S,JOO J Y,et al.Effects of partial replacement of pork meat with chicken or duck meat on the texture,flavor,and consumer acceptance of sausage[J].Journal of Food Quality,2018,2018:6972848.
[4] 晁杰.大豆异黄酮对鸡小肠黏膜上皮细胞的影响[D].硕士学位论文.福州:福建农林大学,2016. CHAO J.Effects of soybean isoflavones on intestinal mucosal epithelial cells in chickens[D].Master's Thesis.Fuzhou:Fujian Agriculture and Forestry University,2016.(in Chinese)
[5] STANGER B Z,DATAR R,MURTAUGH L C,et al.Direct regulation of intestinal fate by Notch[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(35):12443-12448.  
[6] CHEN J,WANG Z Z,KONG L L,et al.Hesperidin[M]//DU G H.Natural small molecule drugs from plants.Singapore:Springer,2018:81-86.
[7] 张明智,隋海娟,张玲玲,等.橙皮苷对功能性消化不良大鼠胃肠运动及Ghrelin表达的影响[J].中药药理与临床,2014,30(2):30-34. ZHANG M Z,SUI H J,ZHANG L L,et al.Effects of hesperidin on functional dyspepsia gastrointestinal motility of rats and the expression of Ghrelin[J].Pharmacology and Clinics of Chinese Materia Medica,2014,30(2):30-34.(in Chinese)
[8] 钟超,祁春节.投入品价格波动对中国柑橘种植面积影响研究[J].价格月刊,2017(2):13-18. ZHONG C,QI C J.Research on the impact of input price fluctuation on orange planting area of China[J].Prices Monthly,2017(2):13-18.(in Chinese)
[9] SIMITZIS P E,SYMEON G K,CHARISMIADOU M A,et al.The effects of dietary hesperidin supplementation on broiler performance and chicken meat characteristics[J].Canadian Journal of Animal Science,2011,91(2):275-282.  
[10] 王海良,崔虎,吕慧源,等.橙皮苷对肉仔鸡生产性能、屠宰性能及免疫机能的影响[J].中国畜牧杂志,2013,49(20):57-60. WANG H L,CUI H,LYU H Y,et al.Performance of hesperidin in production and slaughter of broilers and immune function[J].Chinese Journal of Animal Science,2013,49(20):57-60.(in Chinese)
[11] INSERRA L,PRIOLO A,BIONDI L,et al.Dietary citrus pulp reduces lipid oxidation in lamb meat[J].Meat Science,2014,96(4):1489-1493.  
[12] 赖星.日粮添加橙皮苷和绿原酸对断奶仔猪生长性能与肠道健康的影响[D].硕士学位论文.重庆:西南大学,2016. LAI X.Study on the effects of dietary hesperidin and chlorogenic acid on growth performance and intestinal health in weaned piglets[D].Master's Thesis.Chongqing:Southwest University,2016.(in Chinese)
[13] 刘英.橙皮苷和绿原酸的抗氧化效果以及对仔猪生产性能的影响[D].硕士学位论文.成都:四川农业大学,2008. LIU Y.Study on the antioxidance effeet of hesperidin and chlorogenic acid and on performance of piglets[D].Master's Thesis.Chengdu:Sichuan Agricultural University,2008.(in Chinese)
[14] NAZOK A,REZAEI M,SAYYAHZADEH H.Effect of different levels of dried citrus pulp on performance,egg quality,and blood parameters of laying hens in early phase of production[J].Tropical Animal Health and Production,2010,42(4):737-742.  
[15] 敖·德古里那,周波,肖芳,等柑橘皮渣对断奶羔羊生长性能和血清生化指标的影响及经济效益分析[J].中国饲料,2018(5):60-65. AO·D G L N,ZHOU B,XIAO F,et al.Effects of citrus pulp on growth performance, serum biochemical index of weaned lambs and economic benefit analysis[J].China Feed,2018(5):60-65.(in Chinese)
[16] IRANSHAHI M,REZAEE R,PARHIZ H,et al.Protective effects of flavonoids against microbes and toxins:the cases of hesperidin and hesperetin[J].Life Sciences,2015,137:125-132.
[17] 何雪萍,陈静文,王润莲,等.不同分子质量壳聚糖对黄羽肉鸡生长性能、脂质代谢、屠宰性能及肉品质的影响[J].动物营养学报,2020,32(7):3154-3161. HE X P,CHEN J W,WANG R L,et al.Effects of different molecular weights of chitosan on growth performance,lipid metabolism,slaughter performance and meat quality of yellow-feather broilers[J].Chinese Journal of Animal Nutrition,2020,32(7):3154-3161.(in Chinese)
[18] 曹蓉,侯德兴,宋泽和,等.厚朴总酚对黄羽肉鸡生长性能、肉品质和抗氧化功能的影响[J].动物营养学报,2019,31(12):5696-5706. CAO R,HOU D X,SONG Z H,et al.Effects of total phenols from magnolia officinalis on growth performance,meat quality and antioxidant function of yellow-feather broilers[J].Chinese Journal of Animal Nutrition,2019,31(12):5696-5706.(in Chinese)
[19] 李光全,邓位喜,李佳剑,等.务川黑牛不同杂交组合屠宰性能及肉质研究[J].中国畜牧杂志,2019,55(7):63-66. LI G Q,DENG W X,LI J J,et al.Study on meat yield and meat quality of different hybrid combinations of Wuchuan black cattle[J].Chinese Journal of Animal Science,2019,55(7):63-66.(in Chinese)
[20] GOLIOMYTIS M,KARTSONAS N,CHARISMIADOU M A,et al.The Influence of naringin or hesperidin dietary supplementation on broiler meat quality and oxidative stability[J].PLoS One,2015,10(10):e0141652.
[21] LEE S H,JOO S,RYU Y C.Skeletal muscle fiber type and myofibrillar proteins in relation to meat quality[J].Meat Science,2010,86(1):166-170.  
[22] 许铭洙,张勇,严鸿林,等.大豆异黄酮对骨骼肌发育的影响及其对动物肉品质的调控作用[J].中国畜牧杂志,2019,55(6):15-19,163. XU M Z,ZHANG Y,YAN H L,et al.Effects of soybean isoflavones on skeletal muscle development and its regulation of meat quality in animals[J].Chinese Journal of Animal Science,2019,55(6):15-19,163.(in Chinese)
[23] YOUNG J F,STAGSTED J,JENSEN S K,et al.Ascorbic acid,alpha-tocopherol,and oregano supplements reduce stress-induced deterioration of chicken meat quality[J].Poultry Science,2003,82(8):1343-1351.  
[24] KAMBOH A A,ZHU W Y.Individual and combined effects of genistein and hesperidin supplementation on meat quality in meat-type broiler chickens[J].Journal of the Science of Food and Agriculture,2013,93(13):3362-3367.  
[25] 杨文军,牟春堂,王鹏举,等.饲粮中添加葡萄籽原花青素对羔羊生长性能、屠宰性能、肉品质及血清抗氧化指标的影响[J].动物营养学报,2020,32(6):2755-2764. YANG W J,MOU C T,WANG P J,et al.Effects of dietary supplementation of grape seed proanthocyanidins on growth performance,slaughter performance,meat quality and serum antioxidant indexes of lambs[J].Chinese Journal of Animal Nutrition,2020,32(6):2755-2764.(in Chinese)
[26] 李贞明,余苗,崔艺燕,等.柑橘提取物对黄羽肉鸡器官指数、屠宰性能、肉品质和肌肉抗氧化指标的影响[J].动物营养学报,2019,31(8):3794-3800. LI Z M,YU M,CUI Y Y,et al.Effects of citrus extract on organ indices, slaughter performance, meat quality and muscle antioxidant indices of yellow-feather broilers[J].Chinese Journal of Animal Nutrition,2019,31(8):3794-3800.(in Chinese)
[27] 张俊英,王德宝.自然放牧条件下不同肋骨数乌珠穆沁肉羊屠宰性能与肉品质分析[J].黑龙江畜牧兽医,2019(16):52-55. ZHANG J Y,WANG D B.Slaughter performance and meat quality analysis of Wuzhu muqin mutton sheep with different rib number under natural grazing conditions[J].Heilongjiang Animal Science and Veterinary Medicine,2019(16):52-55.(in Chinese)
[28] 郭照宙.SODm对肉仔鸡生产性能、抗氧化功能和肉质的影响[D].硕士学位论文.哈尔滨:东北农业大学,2018. GUO Z Z.Effects of superoxide dismutase mimetic(SODm)on performance, antioxidant function and meat quality of broilers[D].Master's Thesis.Harbin:Northeast Agricultural University,2018.(in Chinese)
[29] PARHIZ H,ROOHBAKHSH A,SOLTANI F,et al.Antioxidant and anti-inflammatory properties of the citrus flavonoids hesperidin and hesperetin:an updated review of their molecular mechanisms and experimental models[J].Phytotherapy Research,2015,29(3):323-331.  
[30] 赵艳飞,赵新波,徐娟,等.复合型植物提取物对三黄鸡肉品质的影响[J].中国畜牧杂志,2018,54(4):103-108. ZHAO Y F,ZHAO X B,XU J,et al.Influence of compound plant extract on meat quality of yellow-feathered broilers[J].Chinese Journal of Animal Science,2018,54(4):103-108.(in Chinese)
[31] MA W Q,CHENG H Z,ZHAO D H,et al.Effects of dietary Enteromorpha powder supplementation on productive performance,egg quality,and antioxidant performance during the late laying period in Zi geese[J].Poultry Science,2019,99(2):1062-1068.
[32] KAMBOH A A,HANG S Q,KHAN M A,et al.In vivo immunomodulatory effects of plant flavonoids in lipopolysaccharide-challenged broilers[J].Animal,2016,10(10):1619-1625.  
[33] GUO K,REN J N,GU G S,et al.Hesperidin protects against intestinal inflammation by restoring intestinal barrier function and up-regulating treg cells[J].Molecular Nutrition & Food Research,2019,63(11):1800975.
[34] HIRATA A,MURAKAMI Y,SHOJI M,et al.Kinetics of radical-scavenging activity of hesperetin and hesperidin and their inhibitory activity on COX-2 expression[J].Anticancer Research,2005,25(5):3367-3374.
[35] 周建民,马友彪,张海军,等.白酒糟酵母培养物对肉仔鸡生长性能、血清抗氧化指标和肠道形态结构的影响[J].动物营养学报,2019,31(5):2357-2366. ZHOU J M,MA Y B,ZHANG H J,et al.Effects of yeast culture using distiller's grains on growth performance,serum antioxidant indices and intestinal morphology of broilers[J].Chinese Journal of Animal Nutrition,2019,31(5):2357-2366.(in Chinese)
[36] 林成招,马海田,邹思湘,等.大豆异黄酮对大鼠小肠上皮细胞生长及葡萄糖和氨基酸吸收的影响[J].南京农业大学学报,2005,28(1):71-75. LIN C Z,MA H T,ZOU S X,et al.Effects of soybean isoflavone on the growth of the rat epithelial cell and absorption of glucose and amino acids of small intestine[J].Journal of Nanjing Agricultural University,2005,28(1):71-75.(in Chinese)
[37] 从光雷,王强,肖蕴祺,等.饲粮添加橡椀单宁对肉鸡生长性能、屠宰性能、肉品质、抗氧化功能和肠道发育的影响[J/OL].动物营养学报:1-10[2020-10-09].http://kns.cnki.net/kcms/detail/11.5461.S.20200814.1732.010.html. CONG G L,WANG Q,XIAO Y Q,et al.Effects of dietary quercus acutissima carruth tannin on growth performance,slaughter performance,meat quality,antioxidant function and intestinal development of broilers[J/OL].Chinese Journal of Animal Nutrition:1-10[2020-10-09].http://kns.cnki.net/kcms/detail/11.5461.S.20200814.1732.010.html.(in Chinese)
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2