动物营养学报 >

2021 , Vol. 33 >Issue 1: 359 - 369

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.036

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

不同锌源对母羊和羔羊体液免疫及羔羊肠道黏膜组织形态和免疫的影响

  • 田丽娜 ,
  • 颜琼娴 ,
  • 郑梦莉 ,
  • 周传社 ,
  • 谭支良
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  • 1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 畜禽养殖污染控制与资源化技术国家工程实验室, 湖南省畜禽健康养殖工程技术研究中心, 动物营养生理与代谢过程湖南省重点实验室, 长沙 410125;
    2. 中国科学院大学, 北京 100049;
    3. 湖南畜禽安全生产协同创新中心, 长沙 410128
田丽娜(1993-),女,辽宁朝阳人,硕士研究生,研究方向为反刍动物营养。E-mail:783625064@qq.com

收稿日期: 2020-05-27

  网络出版日期: 2021-01-18

基金资助

中国科学院亚热带农业生态研究所青年创新团队（2017QNCXTD_ZCS）；国家重点研发计划（2018YFD0501900）；湖南创新型省份建设（2019RS3021）

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Effects of Different Zinc Sources on Humoral Immunity of Goats and Kid Goats and Intestinal Mucosal Morphology, Immune of Kid Goats

  • TIAN Lina ,
  • YAN Qiongxian ,
  • ZHENG Mengli ,
  • ZHOU Chuanshe ,
  • TAN Zhiliang
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  • 1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Hunan Provincial Key Laboratory of Nutritional Physiology and Metabolic Process, Changsha 410125, China;
    2. University of Chinese Academy of Sciences, Beijing 100094, China;
    3. Hunan Co-Innovation Center for Production Safety, Changsha 410128, China

Received date: 2020-05-27

  Online published: 2021-01-18

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摘要

本试验旨在研究妊娠母羊饲粮中添加不同锌源对母羊和羔羊体液免疫及羔羊肠道组织形态、黏膜免疫功能的影响。选取体重（38.1±9.7）kg、胎次（第2~3胎）相近的怀双羔湘东黑山羊21只，随机分为3组，每组7个重复，每个重复1只羊。各组分别在基础饲粮中添加60 mg/kg的硫酸锌、蛋氨酸螯合锌和甘氨酸螯合锌。基础饲粮中锌含量为22 mg/kg，各试验饲粮中锌含量均为82 mg/kg。预试期7 d，正试期45 d。分别于母羊产前第10天及羔羊出生后的第30、60和100天采集颈静脉血，测定妊娠母羊和羔羊血浆免疫球蛋白（Ig）和细胞因子含量；羔羊在出生后的第100天进行屠宰并采集肠道组织，测定羔羊肠道组织形态及肠道黏膜Ig、细胞因子含量。结果表明：1）母羊产前第10天，甘氨酸螯合锌组血浆白细胞介素（IL）-6和IL-8含量显著高于硫酸锌组和蛋氨酸螯合锌组（P<0.05）。2）羔羊出生后第30天，蛋氨酸螯合锌组血浆IgG含量显著高于甘氨酸螯合锌组（P<0.05）。羔羊出生后第60天，硫酸锌组和蛋氨酸螯合锌组血浆IL-4含量显著高于甘氨酸螯合锌组（P<0.05），蛋氨酸螯合锌组血浆IL-6、IL-22含量显著高于硫酸锌组和甘氨酸螯合锌组（P<0.05），甘氨酸螯合锌组血浆IgG含量显著高于蛋氨酸螯合锌组和硫酸锌组（P<0.05）。羔羊出生后第100天，各组之间血浆细胞因子和Ig含量均差异不显著（P>0.05）。3）各组之间空肠和回肠的绒毛宽度、绒毛高度、隐窝深度和绒毛高度/隐窝深度无显著差异（P>0.05）。4）蛋氨酸螯合锌组和甘氨酸螯合锌组空肠黏膜IL-6、IL-22、IL-23和IgG含量显著高于硫酸锌组（P<0.05），甘氨酸螯合锌组空肠黏膜IL-4含量显著高于硫酸锌组和蛋氨酸螯合锌组（P<0.05），蛋氨酸螯合锌组空肠黏膜分泌型免疫球蛋白A（sIgA）含量显著高于硫酸锌组和甘氨酸螯合锌组（P<0.05）。蛋氨酸螯合锌组和甘氨酸螯合锌组回肠黏膜IL-4、IL-6、IL-23、IgM和IgG含量显著高于硫酸锌组（P<0.05），蛋氨酸螯合锌组回肠黏膜sIgA含量显著高于硫酸锌组和甘氨酸螯合锌组（P<0.05），且蛋氨酸螯合锌组回肠黏膜IgM和sIgA含量显著高于甘氨酸螯合锌组（P<0.05）。由此可见，妊娠母羊饲粮中添加蛋氨酸螯合锌和甘氨酸螯合锌能够改善羔羊体液免疫和肠道黏膜免疫功能，且蛋氨酸螯合锌优于甘氨酸螯合锌。

关键词： 氨基酸螯合锌; 羔羊; 免疫球蛋白; 细胞因子

本文引用格式

田丽娜 , 颜琼娴 , 郑梦莉 , 周传社 , 谭支良 . 不同锌源对母羊和羔羊体液免疫及羔羊肠道黏膜组织形态和免疫的影响[J]. 动物营养学报, 2021 , 33(1) : 359 -369 . DOI: 10.3969/j.issn.1006-267x.2021.01.036

Abstract

This experiment was conducted to study the effects of dietary different zinc sources of pregnant goats on humoral immunity of goats and kid goats and intestinal morphology, mucosal immune function of kid goats. Twenty-one Xiangdong black goats with twin pregnancies, similar parity (the 2 to 3 fetus) and body weight [(38.1±9.7) kg] were randomly divided into 3 groups with 7 replicates per group and 1 goat per replicate. Goats in 3 groups were fed basal diets supplemented with 60 mg/kg zinc sulfate, methionine chelating zinc (Zn-Met) and glycine chelating zinc (Zn-Gly), respectively. The zinc content of the basal diet was 22 mg/kg, and the zinc content of each experimental diet was 82 mg/kg. The pre-experimental period lasted for 7 days, and the experimental period lasted for 45 days. The jugular veinal blood of pregnant goats at day 10 before birth, and newborn goats at days 30, 60 and 90 was collected, and measured the contents of immunoglobulins (Ig) and cytokines in plasma of goats and kid goats. The kid goats were slaughtered at day 100 and the intestinal tissue were collected to determine the intestinal morphology and contents of Ig and cytokines in intestinal mucosa. The results showed as follows: 1) on day 10 of goats before delivery, the contents of interleukin (IL)-6 and IL-8 in plasma of Zn-Gly group were significantly higher than those of zinc sulfate group and Zn-Met group (P<0.05). 2) On day 30 after kid goats' birth, the plasma IgG content of Zn-Met group was significantly higher than that of Zn-Gly group (P<0.05). On day 60 after kid goats birth, the plasma IL-4 content of zinc sulfate group and Zn-Met group was significantly higher than that of Zn-Gly group (P<0.05), the contents of IL-6 and IL-22 in plasma of Zn-Met group were significantly higher than those of zinc sulfate group and Zn-Gly group (P<0.05), and the plasma IgG content of Zn-Gly group was significantly higher than that of sulfate group and Zn-Met group (P<0.05). On day 100 after kid goats birth, there were no significant differences on contents of cytokines and Ig in plasma among all groups (P>0.05). 3) There were no significant differences on villus width, villus height, crypt depth and villus height/crypt depth in jejunum and ileum among all groups (P>0.05). 4) The contents of IL-6, IL-22, IL-23 and IgG in jejunal mucosa of Zn-Met group and Zn-Gly group were significantly higher than those of zinc sulfate group (P<0.05), the jejunal mucosa IL-4 content of Zn-Gly group was significantly higher than that of zinc sulfate group and Zn-Met group (P<0.05), and the jejunal mucosa secretory immunoglobulin A (sIgA) content of Zn-Met group was significantly higher than that of zinc sulfate group and Zn-Gly group (P<0.05). The contents of IL-4, IL-6, IL-23, IgM and IgG in ileal mucosa of Zn-Met group and Zn-Gly group were significantly higher than those of zinc sulfate group (P<0.05), the ileal mucosa sIgA content of Zn-Met group was significantly higher than that of zinc sulfate group and Zn-Gly group (P<0.05), and the contents of IgM and sIgA in ileal mucosa of Zn-Met group were significantly higher than those of Zn-Gly group (P<0.05). In conclusion, dietary supplementation of Zn-Met and Zn-Gly for pregnant goats can improve the humoral immunity and intestinal mucosal immune function of kid goats, and the Zn-Met is better than Zn-Gly.

Key words： amino acid chelating zinc; kid goats; immunoglobulins; cytokines

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