反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

饲粮中添加不同比例黄芪副产物对绵羊脾脏中Toll样受体2和白细胞介素-1受体相关激酶4表达的影响

  • 王霞 ,
  • 陈智丽 ,
  • 马焕交 ,
  • 李讨讨 ,
  • 刘婷 ,
  • 马友记
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  • 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 新疆哈密市伊吾县林业和草原局, 哈密 839300;
    3. 甘肃省肉羊繁育生物技术工程实验室, 民勤 733300
王霞(1993-),女,甘肃会宁人,硕士研究生,动物遗传育种与繁殖专业。E-mail:wangxiaandzisu@163.com

收稿日期: 2020-06-27

  网络出版日期: 2021-01-18

基金资助

国家重点研发计划课题(2018YFD0502100)

Effects of Dietary Different Proportions of Astragalus By-Products on Expression of Toll-Like Receptor 2 and Interleukin-1 Receptor Associated Kinase 4 in Spleen of Sheep

  • WANG Xia ,
  • CHEN Zhili ,
  • MA Huanjiao ,
  • LI Taotao ,
  • LIU Ting ,
  • MA Youji
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  • 1. College of Animal Science & Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Forestry and Grassland Bureau of Yiwu County, Hami City, Xinjiang, Hami 839300, China;
    3. Gansu Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology, Minqin 733300, China

Received date: 2020-06-27

  Online published: 2021-01-18

摘要

本试验旨在研究基础饲粮中添加不同比例黄芪副产物对绵羊脾脏中Toll样受体2(TLR2)和白细胞介素-1受体相关激酶4(IRAK4)表达的影响。选取健康、体重[(27.43±3.61)kg]接近的3月龄澳洲白与湖羊杂交F1公羔68只,随机分为4组,每组17只羊。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加2%(2%组)、4%(4%组)、6%(6%组)的黄芪副产物。预试期10 d,正试期60 d。结果表明:1)各组绵羊的终末体重、平均日增重和平均干物质采食量差异不显著(P>0.05)。2%组羔羊的脾脏指数显著高于对照组、4%组和6%组(P<0.05),而4%组、6%组与对照组之间差异不显著(P>0.05)。2)2%组绵羊脾脏中TLR2和IRAK4的mRNA和蛋白相对表达量显著高于对照组、4%组和6%组(P<0.05)。3)免疫组化染色发现TLR2蛋白主要定位在饲喂不同比例黄芪副产物绵羊脾脏红髓的脾窦中,IRAK4蛋白主要定位在饲喂不同比例黄芪副产物绵羊脾脏红髓的脾索中。由此可见,基础饲粮中添加2%的黄芪副产物不仅可以增加绵羊的脾脏指数,还可以上调脾脏中TLR2和IRAK4基因的表达,从而在机体的免疫调节中发挥作用。

本文引用格式

王霞 , 陈智丽 , 马焕交 , 李讨讨 , 刘婷 , 马友记 . 饲粮中添加不同比例黄芪副产物对绵羊脾脏中Toll样受体2和白细胞介素-1受体相关激酶4表达的影响[J]. 动物营养学报, 2021 , 33(1) : 379 -389 . DOI: 10.3969/j.issn.1006-267x.2021.01.038

Abstract

This experiment was conducted to study the effects of dietary different proportions of astragalus by-products on expression of Toll-like receptor 2 (TLR2) and interleukin-1 receptor associated kinase 4 (IRAK4) in spleen of sheep. Sixty-eight healthy Australian white×Hu sheep crossbred F1 male lambs with similar body weight of (27.43±3.61) kg were randomly divided into 4 groups with 17 lambs per group.Lambs in the control group were fed a basal diet, and the others experimental groups were fed the basal diets supplemented with 2% (2% group), 4% (4% group) and 6% (6% group) Astragalus by-products, respectively. The pre-experimental period lasted for 10 days, and the experimental period lasted for 60 days. The results were as follows: 1) there were no significantly differences in the final body weight, average daily gain and average dry matter intake of sheep among all groups (P>0.05). The spleen index of sheep of 2% group was significantly higher than that of control group, 4% group and 6% group (P<0.05), while there was no significant difference among 4% group, 6% group and control group (P>0.05). 2) The mRNA and protein relative expression levels of TLR2 and IRAK4 in spleen of 2% group were significantly higher than those of control group, 4% group and 6% group (P<0.05). 3) Immunohistochemical staining showed that the TLR2 protein was mainly located in the splenic sinus of splenic red pulp of sheep fed with different proportions of Astragalus by-products, and the IRAK4 protein was mainly located in the splenic cord of splenic red pulp of sheep fed with different proportions of Astragalus by-products. In conclusion, the basic diets supplemented with 2% Astragalus by-products can not only increase the spleen index, but also up-regulate the expression levels of TLR2 and IRAK4 genes in spleen, thereby playing a role in immune regulation of the body.

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