动物营养学报 >

2021 , Vol. 33 >Issue 1: 436 - 447

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.01.044

水产动物营养与饲料 AQUATIC ANIMAL NUTRITION AND FEED

氨氮胁迫下饥饿与复投喂对黄颡鱼肝脏中脂质代谢相关酶活性及相关基因表达的影响

  • 刘嘉欣 ,
  • 张木子 ,
  • 黎明 ,
  • 谢雨欣 ,
  • 钱云霞 ,
  • 王日昕
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  • 宁波大学海洋学院, 宁波 315211
刘嘉欣(1995-),男,山东济南人,硕士研究生,从事水生动物营养与免疫研究。E-mail:1048880723@qq.com

收稿日期: 2020-06-16

  网络出版日期: 2021-01-18

基金资助

国家自然科学基金项目（31872541，31872222）；浙江省省属高校基本科研业务费专项资金（SJLY2020009）

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Effects of Starvation and Re-Feeding on Enzyme Activities and Gene Expression Involved in Liver Lipid Metabolism of Yellow Catfish (Pelteobagrus fulvidraco) under Ammonia Nitrogen Stress

  • LIU Jiaxin ,
  • ZHANG Muzi ,
  • LI Ming ,
  • XIE Yuxin ,
  • QIAN Yunxia ,
  • WANG Rixin
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  • School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2020-06-16

  Online published: 2021-01-18

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摘要

为了研究氨氮胁迫下饥饿与复投喂对黄颡鱼脂质代谢的影响，将360尾黄颡鱼幼鱼[初始体重为（14.95±0.03）g]随机分配到12个养殖桶中，每桶30尾。对照组人工饱食投喂42 d，试验组饥饿14 d后恢复投喂28 d。对照组和试验组分别被暴露到总氨氮浓度为0（低氨氮处理，正常养殖环境）和5.70 mg/L（高氨氮处理，氨氮胁迫）的水体中，每组分配3桶试验鱼。结果显示：饥饿14 d后，高氨氮处理黄颡鱼肝脏中6-磷酸葡萄糖酸脱氢酶（6PGD）、脂肪酸合酶（FAS）活性以及6PGD、葡萄糖-6-磷酸脱氢酶（G6PD）、FAS、胆固醇调节元件结合蛋白-1（SREBP-1）、过氧化物酶体增殖物激活受体α（PPARα）和过氧化物酶体增殖物激活受体γ（PPARγ）基因的mRNA相对表达量显著低于低氨氮处理（P<0.05），而肝脏中肉碱棕榈酰转移酶（CPT）、脂蛋白脂酶（LPL）活性以及肉碱棕榈酰转移酶1（CPT1）和LPL基因的mRNA相对表达量则显著高于低氨氮处理（P<0.05）；在氨氮胁迫或正常养殖环境下，饥饿14 d后，试验组黄颡鱼肝脏中6PGD、FAS活性以及6PGD、G6PD、FAS、SREBP-1和PPARγ基因的mRNA相对表达量显著低于对照组（P<0.05），而CPT、LPL活性以及PPARα、CPT1和LPL基因的mRNA相对表达量显著高于对照组（P<0.05）；恢复投喂28 d后，高氨氮处理黄颡鱼肝脏中6PGD、FAS活性以及6PGD、G6PD、FAS和PPARα基因的mRNA相对表达量显著低于低氨氮处理（P<0.05），而肝脏中CPT、LPL活性以及CPT1和LPL基因的mRNA相对表达量则显著高于低氨氮组（P<0.05）；在氨氮胁迫或正常养殖环境下，恢复投喂28 d后，试验组黄颡鱼肝脏中6PGD活性以及6PGD和G6PD基因的mRNA相对表达量显著高于对照组（P<0.05），而肝脏中LPL活性显著低于对照组（P<0.05）；此外，在氨氮胁迫下，试验组黄颡鱼肝脏中FAS、SREBP-1和PPARγ基因的mRNA相对表达量显著高于对照组（P<0.05），而LPL基因的mRNA相对表达量则显著低于对照组（P<0.05）。综上可知，氨氮胁迫和饥饿胁迫均会促进黄颡鱼的脂质分解代谢，并抑制脂质合成代谢；氨氮胁迫下饥饿后复投喂能够恢复黄颡鱼的脂质代谢稳态。

关键词： 黄颡鱼; 氨氮胁迫; 饥饿胁迫; 复投喂; 脂质代谢; 酶活性; 基因表达

本文引用格式

刘嘉欣 , 张木子 , 黎明 , 谢雨欣 , 钱云霞 , 王日昕 . 氨氮胁迫下饥饿与复投喂对黄颡鱼肝脏中脂质代谢相关酶活性及相关基因表达的影响[J]. 动物营养学报, 2021 , 33(1) : 436 -447 . DOI: 10.3969/j.issn.1006-267x.2021.01.044

Abstract

This experiment was conducted to study the effects of starvation and re-feeding on lipid metabolism of yellow catfish (Pelteobagrus fulvidraco) under ammonia nitrogen stress. Three hundred and thirty-six yellow catfish [initial body weight: (14.95±0.03) g] were randomly distributed into 12 culture buckets with 30 fish per bucket. The fish in the control group were fed on satiation for 42 days, and the fish in the trial group were starved for 14 days, then re-feeding to satiation for 28 days. The control group and trial group were exposed to 0 (low ammonia nitrogen treatment, normal culture environment) and 5.70 mg/L ammonia nitrogen (high ammonia nitrogen treatment, ammonia nitrogen stress), respectively, and each group had 3 buckets of fish. The results showed as follows: after 14 days of starvation, the activities of 6-phosphogluconate dehydrogenase (6PGD) and fatty acid synthase (FAS), and the mRNA relative expression levels of 6PGD, glucose 6-phosphate dehydrogenase (G6PD), FAS, sterol-regulatory element binding protein-1 (SREBP-1), peroxisome proliferator-activated receptor α (PPARα) and peroxisome proliferator-activated receptor γ (PPARγ) genes in the liver of fish in the high ammonia nitrogen treatment were significantly lower than those of fish in the low ammonia nitrogen treatment, but the activities of carnitine palmitoyltransferase (CPT) and lipoprotein lipase (LPL), and the mRNA relative expression levels of carnitine palmitoyltransferase 1 (CPT1) and LPL genes were significantly higher than those of fish in the low ammonia nitrogen treatment (P<0.05). Under the ammonia nitrogen stress or normal culture environment, after 28 days of starvation, the activities of 6PGD and FAS, and the mRNA relative expression levels of 6PGD, G6PD, FAS, SREBP-1 and PPARγ genes in the liver of fish in the trial group were significantly lower than those of fish in the control group (P<0.05), but the activities of CPT and LPL, and the mRNA relative expression levels of PPARα, CPT1 and LPL genes were significantly higher than those of fish in the control group (P<0.05). After 28 days of re-feeding, the activities of 6PGD and FAS, and the mRNA relative expression levels of 6PGD, G6PD, FAS and PPARα genes in the liver of fish in the high ammonia nitrogen treatment were significantly lower than those of fish in the low ammonia nitrogen treatment (P<0.05), but the activities of CPT and LPL, and the mRNA relative expression levels of CPT1 and LPL genes were significantly higher than those of fish in low ammonia nitrogen treatment (P<0.05). Under the ammonia nitrogen stress or normal culture environment, after 28 days of re-feeding, the activity of 6PGD and the mRNA relative expression levels of 6PGD and G6PD genes in the liver of fish in the trial group were significantly higher than those of fish in the control group (P<0.05), but the activity of LPL was significantly lower than that of fish in the control group (P<0.05). Moreover, under the ammonia nitrogen stress, the mRNA relative expression levels of FAS, SREBP-1 and PPARγ genes in the liver of fish in the trial group were significantly higher than those of fish in the control group (P<0.05), but the mRNA relative expression level of LPL gene was significantly lower than that of fish in the control group (P<0.05). The results indicate that both ammonia nitrogen stress and starvation stress can promote the lipid catabolism and inhibit the lipid anabolism of yellow catfish, and re-feeding after starvation can restore the lipid metabolism homeostasis of yellow catfish under ammonia nitrogen stress.

Key words： yellow catfish (Pelteobagrus fulvidraco); ammonia nitrogen stress; starvation stress; re-feeding; lipid metabolism; enzyme activity; gene expression

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