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小肽对大黄鱼仔鱼生长和小肠发育的影响及其在低温胁迫下的抗氧化应激效应

  • 李文成 ,
  • 赵旭民 ,
  • 张延军 ,
  • 宋炜 ,
  • 曾霖 ,
  • 张惠 ,
  • 赵向炯
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  • 1. 浙江海洋大学国家海洋设施养殖工程技术研究中心, 舟山 316000;
    2. 中国水产科学研究院东海水产研究所, 上海 200090;
    3. 浙江华太生物科技有限公司, 义乌 322005;
    4. 青岛海洋科学与技术试点国家实验室, 青岛 266237
李文成(1995-),男,山东济南人,硕士研究生,从事水产养殖和营养研究。E-mail:Litian731418@163.com

收稿日期: 2020-06-22

  网络出版日期: 2021-01-18

基金资助

科技部国家重点研发计划——蓝色粮仓科技创新专项(2019YFD0900904); 山东省支持青岛海洋科学与技术试点国家实验室重大科技专项(2018SDKJ0303-4);浙江省自然科学基金项目(LY18D060008);企业合作项目"基于氨基酸蛋白营养液的水产肥水培藻关键技术研发"(22248002219)

Effects of Small Peptides on Growth and Intestine Development of Larval Large Yellow Croaker (Larimichthys crocea) and Its Anti-Oxidative Stress Effect under Low-Temperature Stress

  • LI Wencheng ,
  • ZHAO Xumin ,
  • ZHANG Yanjun ,
  • SONG Wei ,
  • ZENG Lin ,
  • ZHANG Hui ,
  • ZHAO Xiangjiong
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  • 1. National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316000, China;
    2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
    3. Zhejiang Huatai Biological Technology Co., Ltd., Yiwu 322005, China;
    4. Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China

Received date: 2020-06-22

  Online published: 2021-01-18

摘要

本试验分为生长试验和低温胁迫试验2部分。先分别投喂大黄鱼(Larimichthys crocea)仔鱼经不同浓度(0、0.5、1.0、2.0和3.0 mL/m3)小肽营养强化后的轮虫和卤虫12 d,以探讨小肽对大黄鱼仔鱼生长和小肠发育的影响;再将大黄鱼仔鱼暴露在温度为12℃的水体中24 h,以探讨小肽对低温胁迫下大黄鱼仔鱼抗氧化能力和非特异性免疫力的影响。结果显示:不同浓度小肽均显著增加了轮虫和卤虫的必需氨基酸和总氨基酸含量(P<0.05),显著提高了大黄鱼仔鱼的体长及小肠绒毛高度、数量和组织面积(P<0.05)。小肽显著提高低温胁迫下大黄鱼仔鱼的谷胱甘肽(GSH)含量以及肝脏抗氧化酶[铜锌超氧化物歧化酶(Cu/Zn-SOD)、锰超氧化物歧化酶(Mn-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶-1a(GPx-1a)、谷胱甘肽过氧化物酶-1b(GPx-1b)和谷胱甘肽还原酶(GR)]基因和非特异性免疫酶[c型溶菌酶(c-type LZM)、g型溶菌酶(g-type LZM)和碱性磷酸酶(AKP)]基因表达水平,显著降低活性氧(ROS)含量(P<0.05)。核转录因子NF-E2相关因子2(Nrf2)和核转录因子-κB(NF-κB)基因表达水平均与抗氧化酶基因和非特异性免疫酶基因表达水平呈显著正相关(P<0.05),并均与ROS含量呈显著负相关(P<0.05)。综上所述,小肽能够提高轮虫和卤虫的营养价值,从而改善大黄鱼仔鱼的生长和小肠发育;小肽通过诱导Nrf2和NF-κB的表达来增加抗氧化酶基因和非特异性免疫酶基因表达水平,从而缓解低温胁迫诱导的氧化损伤。

本文引用格式

李文成 , 赵旭民 , 张延军 , 宋炜 , 曾霖 , 张惠 , 赵向炯 . 小肽对大黄鱼仔鱼生长和小肠发育的影响及其在低温胁迫下的抗氧化应激效应[J]. 动物营养学报, 2021 , 33(1) : 572 -583 . DOI: 10.3969/j.issn.1006-267x.2021.01.058

Abstract

This experiment included two parts: growth trial and low-temperature stress trial. Larval large yellow croakers (Larimichthys crocea) were fed rotifers and Artemia nauplii fortified with different concentrations (0, 0.5, 1.0, 2.0 and 3.0 mL/m3) of small peptides for 12 d, which aimed to evaluate the effects of small peptides on growth and intestine development of larval large yellow croaker. Then, larval large yellow croakers were exposed to low-temperature environment (at 12 ℃) for 24 h, which aimed to evaluate the antioxidant ability and non-specific immunity of larvae large yellow croaker under low-temperature stress. The results showed that different concentrations of small peptides significantly increased the contents of essential amino acids and total amino acids of rotifers and Artemia nauplii. Fish fed rotifers and Artemia nauplii fortified with small peptides significantly increased body length, villi height, villi number and tissue area (P<0.05). Small peptides significantly reduced oxygen species (ROS) content (P<0.05), and significantly increased GSH content and the expression levels of liver antioxidant-related genes [copper-zinc superoxide dismutase (Cu/Zn-SOD and manganese superoxide dismutase (Mn-SOD), catalase (CAT), glutathione peroxidase-1a (GPx-1a), glutathione peroxidase-1b (GPx-1b) and glutathione reductase (GR)] and non-specific immune-related genes [c-type lysozyme (c-type LZM), g-type lysozyme (g-type LZM) and alkaline phophatase (AKP)] when fish exposed to low temperature (P<0.05). Nuclear transcription factors NF-E2-related nuclear factor 2 (Nrf2) and nuclear transcription factor-κB (NF-κB)] gene expression levels were significantly positively correlated with the expression levels of antioxidant enzyme genes and immune enzyme genes (P<0.05), and both of them were significantly negatively correlated with ROS content (P<0.05). In conclusion, small peptides can increase the nutritional value of rotifers and Artemia nauplii, and improve the growth and intestine development of larval large yellow croaker; small peptides can increase the expression levels of antioxidant enzyme genes and immune enzyme genes by inducing the expression of Nrf2 and NF-κB genes, and ameliorate the oxidative damage of large yellow croaker larvae induced by low-temperature stress.

参考文献

[1] AO J Q,MU Y N,XIANG L X,et al.Genome sequencing of the perciform fish Larimichthys crocea provides insights into molecular and genetic mechanisms of stress adaptation[J].PLoS Genetics,2015,11(4):e1005118.
[2] 刘家富.大黄鱼养殖与生物学[M].厦门:厦门大学出版社,2013:1-6. LIU J F.Culture and biology of large yellow croaker[M].Xiamen:Xiamen University Press,2013:1-6.(in Chinese)
[3] LIU C,SHEN W L,HOU C C,et al.Low temperature-induced variation in plasma biochemical indices and aquaglyceroporin gene expression in the large yellow croaker Larimichthys crocea[J].Scientific Reports,2019,9(1):2717.
[4] YE C X,WAN F,SUN Z Z,et al.Effect of phosphorus supplementation on cell viability,anti-oxidative capacity and comparative proteomic profiles of puffer fish (Takifugu obscurus) under low temperature stress[J].Aquaculture,2016,452:200-208..
[5] LUSHCHAK V I.Environmentally induced oxidative stress in aquatic animals[J].Aquatic Toxicology,2011,101(1):13-30.  
[6] BANH S,WIENS L,SOTIRI E,et al.Mitochondrial reactive oxygen species production by fish muscle mitochondria:potential role in acute heat-induced oxidative stress[J].Comparative Biochemistry and Physiology,Part B:Biochemistry and Molecular Biology,2016,191:99-107.
[7] HALLIWELL B.Free radicals and antioxidants:updating a personal view[J].Nutrition Reviews,2012,70(5):257-265.  
[8] SRIKANTH K,PEREIRA E,DUARTE A C,et al.Glutathione and its dependent enzymes' modulatory responses to toxic metals and metalloids in fish-a review[J].Environmental Science and Pollution Research,2013,20(4):2133-2149.  
[9] SAREILA O,KELKKA T,PIZZOLLA A,et al.NOX2 complex-derived ROS as immune regulators[J].Antioxidants & Redox Signaling,2011,15(8):2197-2208.  
[10] JIA R,DU J L,CAO L P,et al.Antioxidative,inflammatory and immune responses in hydrogen peroxide-induced liver injury of tilapia (GIFT,Oreochromis niloticus)[J].Fish & Shellfish Immunology,2019,84:894-905.
[11] SANT K E,HANSEN J M,WILLIAMS L M,et al.The role of Nrf1 and Nrf2 in the regulation of glutathione and redox dynamics in the developing zebrafish embryo[J].Redox Biology,2017,13:207-218.
[12] NAPETSCHNIG J,WU H.Molecular basis of NF-κB signaling[J].Annual Review of Biophysics,2013,42:443-468.
[13] SHEN W L,MATSUI T.Intestinal absorption of small peptides:a review[J].International Journal of Food Science & Technology,2019,54(6):1942-1948.  
[14] LEÓN R,RUIZ M,VALERO Y,et al.Exploring small cationic peptides of different origin as potential antimicrobial agents in aquaculture[J].Fish & Shellfish Immunology,2020,98:720-727.
[15] WANG T,CHENG Y,CHEN X,et al.Effects of small peptides,probiotics,prebiotics,and synbiotics on growth performance,digestive enzymes,and oxidative stress in orange-spotted grouper,Epinephelus coioides,juveniles reared in artificial seawater[J].Chinese Journal of Oceanology and Limnology,2017,35(1):89-97.  
[16] 李日美,申光荣,黄放,等.小肽对凡纳滨对虾幼虾生长、体成分、非特异性免疫力及抗病力的影响[J].动物营养学报,2018,30(8):3082-3090. LI R M,SHEN G R,HUANG F,et al.Effects of small peptides on growth,body composition,non-specific immunity and disease resistance of juvenile Litopenaeus vannamei[J].Chinese Journal of Animal Nutrition,2018,30(8):3082-3090.(in Chinese)
[17] ZENG L,AI C X,ZHENG J L,et al.Cu pre-exposure alters antioxidant defense and energy metabolism in large yellow croaker Larimichthys crocea in response to severe hypoxia[J].Science of the Total Environment,2019,687:702-711.
[18] ZENG L,ZHENG J L,WANG Y H,et al.The role of Nrf2/Keap1 signaling in inorganic mercury induced oxidative stress in the liver of large yellow croaker Pseudosciaena crocea[J].Ecotoxicology and Environmental Safety,2016,132:345-352.
[19] ZENG L,AI C X,ZHANG J S,et al.Pre-hypoxia exposure inhibited copper toxicity by improving energy metabolism,antioxidant defence and mitophagy in the liver of the large yellow croaker Larimichthys crocea[J].Science of the Total Environment,2020,708:134961.
[20] WU C W,ZHANG D,KAN M Y,et al.The draft genome of the large yellow croaker reveals well-developed innate immunity[J].Nature Communications,2014,5:5227.
[21] REGOLI F,GIULIANI M E.Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organisms[J].Marine Environmental Research,2014,93:106-117.
[22] 李杰.日粮中大豆蛋白小肽添加水平对虹鳟生产性能、消化及免疫功能的影响[D].硕士学位论文.雅安:四川农业大学,2017:16-29. LI J.Effects of dietary small peptides levels on growth performance,muscle quality,physiological and biochemical indexes in juvenile starry flounder (Platichthys stellatus)[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2017:16-29. in Chinese)
[23] 王际英,姜柯君,夏斌,等.小肽对星斑川鲽幼鱼消化酶活性、抗氧化能力和生化组成的影响[J].中国水产科学,2014,21(6):1154-1164. WANG J Y,JIANG K J,XIA B,et al.Effects of small dietary peptides on digestive enzyme activity,antioxidative capability,and biochemical composition in tissues of juvenile starry flounder Platichthys stellatus[J].Journal of Fishery Sciences of China,2014,21(6):1154-1164.(in Chinese)
[24] UMENO A,BIJU V,YOSHIDA Y.In vivo ROS production and use of oxidative stress-derived biomarkers to detect the onset of diseases such as Alzheimer's disease,Parkinson's disease,and diabetes[J].Free Radical Research,2017,51(4):413-427.  
[25] ZENG L,AI C X,WANG Y H,et al.Abrupt salinity stress induces oxidative stress via the Nrf2-Keap1 signaling pathway in large yellow croaker Pseudosciaena crocea[J].Fish Physiology and Biochemistry,2017,43(4):955-964.  
[26] ZENG L,AI C X,ZHANG J S,et al.Toxicological effects of waterborne Zn on the proximal and distal intestines of large yellow croaker Larimichthys crocea[J].Ecotoxicology and Environmental Safety,2019,174:324-333.
[27] ZENG L,AI C X,ZHANG J S,et al.Essential element Cu and non-essential element Hg exposures have different toxicological effects in the liver of large yellow croaker[J].Marine Pollution Bulletin,2019,139:6-13.
[28] IGHODARO O M,AKINLOYE O A.First line defence antioxidants-superoxide dismutase (SOD),catalase (CAT) and glutathione peroxidase (GPX):their fundamental role in the entire antioxidant defence grid[J].Alexandria Journal of Medicine,2018,54(4):287-293.  
[29] MONTEIRO D A,RANTIN F T,KALININ A L.Inorganic mercury exposure:toxicological effects,oxidative stress biomarkers and bioaccumulation in the tropical freshwater fish matrinxã,Brycon amazonicus (Spix and Agassiz,1829)[J].Ecotoxicology,2010,19:105-123.
[30] TACCHI L,BICKERDIKE R,DOUGLAS A,et al.Transcriptomic responses to functional feeds in Atlantic salmon (Salmo salar)[J].Fish & Shellfish Immunology,2011,31(5):704-715.  
[31] OLSEN R E,MYKLEBUST R,KRYVI H,et al.Damaging effect of dietary inulin on intestinal enterocytes in Arctic charr (Salvelinus alpinus L.)[J].Aquaculture Research,2011,32(11):931-934.
[32] DODSON M,REDMANN M,RAJASEKARAN N S,et al.KEAP1-NRF2 signalling and autophagy in protection against oxidative and reductive proteotoxicity[J].Biochemical Journal,2015,469(3):347-355.  
[33] WANG Y T,MANDAL A K,SON Y O,et al.Roles of ROS,Nrf2,and autophagy in cadmium-carcinogenesis and its prevention by sulforaphane[J].Toxicology and Applied Pharmacology,2018,353:23-30.
[34] DOS-SANTOS-PEREIRA M,GUIMARÃES F S,DEL-BEL E,et al.Cannabidiol prevents LPS-induced microglial inflammation by inhibiting ROS/NF-κB-dependent signaling and glucose consumption[J].Glia,2020,68(3):561-573.  
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