禽营养与饲料 POULTRY NUTRITION AND FEED

苯甲酸对球虫攻毒肉鸡生长性能、免疫功能及血清抗氧化能力的影响

  • 黄灵杰 ,
  • 彭焕伟 ,
  • 张克英 ,
  • 白世平 ,
  • 王建萍 ,
  • 曾秋凤 ,
  • 丁雪梅
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  • 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 成都 611130
黄灵杰(1993-),女,四川通江人,硕士研究生,从事家禽营养研究。E-mail:13438286483@163.com

收稿日期: 2020-08-21

  网络出版日期: 2021-03-18

基金资助

"十三五"育种攻关计划项目(2016NYZ0052)

Effects of Benzoic Acid on Growth Performance, Immune Function and Serum Antioxidant Capacity of Broilers Challenged by Coccidia

  • HUANG Lingjie ,
  • PENG Huanwei ,
  • ZHANG Keying ,
  • BAI Shiping ,
  • WANG Jianping ,
  • ZENG Qiufeng ,
  • DING Xuemei
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  • Key Laboratory of Animal Disease Resistance and Nutrition, Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2020-08-21

  Online published: 2021-03-18

Supported by

 

摘要

本试验旨在研究苯甲酸对球虫攻毒肉鸡生长性能、免疫功能及血清抗氧化能力的影响。试验采用2×3双因子设计,选用540只1日龄健康爱拔益加(AA)白羽肉公鸡,2种攻毒处理(灌服无菌生理盐水或30倍球虫疫苗),3种苯甲酸源[饲粮中不添加苯甲酸(N-B)、添加0.05%肠溶型缓释苯甲酸(ES-B)或0.10%未包被苯甲酸(NC-B)],共计6个组,每组6个重复,每个重复15只鸡。试验期42 d。结果表明:1)饲粮中添加苯甲酸有提高肉鸡42日龄时体重(BW)和1~42日龄时体增重(BWG)的趋势(P=0.058,P=0.057);球虫攻毒显著降低BW(除14日龄时外)、BWG和平均采食量(AFI)(除1~14日龄时外)(P<0.05),显著提高15~21日龄时的料重比(F/G)(P<0.05),且N-B组的生长性能下降幅度大于ES-B和NC-B组。2)饲粮中添加苯甲酸显著提高肉鸡21日龄时的血清免疫球蛋白M(IgM)含量、胸腺指数和42日龄时的血清免疫球蛋白A(IgA)含量(P<0.05),有降低42日龄时血清一氧化氮(NO)含量的趋势(P=0.068);球虫攻毒显著提高42日龄时的血清白蛋白(ALB)含量,显著降低21日龄时的血清碱性磷酸酶(ALP)活性和42日龄时的血清IgM含量(P<0.05);饲粮中添加苯甲酸与球虫攻毒对21日龄时的血清IgM含量有显著交互作用(P<0.05),表现为在球虫攻毒条件下,饲粮中添加苯甲酸提高血清IgM含量,且以ES-B组效果最佳。3)饲粮中添加苯甲酸显著提高肉鸡21日龄时的血清谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05),显著降低血清丙二醛(MDA)含量(P<0.05);饲粮中添加苯甲酸与球虫攻毒对42日龄时的血清MDA含量和GSH-Px活性有显著交互作用(P<0.05),表现为在球虫攻毒条件下,饲粮中添加苯甲酸提高血清GSH-Px活性及降低血清MDA含量,且以NC-B组效果最佳。由此得出,饲粮中添加苯甲酸可提高肉鸡的生长性能;在球虫攻毒条件下,饲粮中添加苯甲酸可通过改善机体健康和免疫功能,从而缓解球虫攻毒造成的生长性能下降,且以添加0.05% ES-B的改善效果较好。

本文引用格式

黄灵杰 , 彭焕伟 , 张克英 , 白世平 , 王建萍 , 曾秋凤 , 丁雪梅 . 苯甲酸对球虫攻毒肉鸡生长性能、免疫功能及血清抗氧化能力的影响[J]. 动物营养学报, 2021 , 33(3) : 1396 -1407 . DOI: 10.3969/j.issn.1006-267x.2021.03.022

Abstract

This study was to investigate the effects of benzoic acid (BA) on growth performance, immune function and serum antioxidant capacity of broilers challenged by coccidia. A 2×3 double factors design was used to select 540 one-day-old healthy Arbor Acres (AA) white-feathered male broilers, 2 kinds of challenge treatments (administered sterile saline or 30-fold coccidiosis vaccine), 3 kinds of benzoic acid sources [no adding BA (N-B) in the diet, adding 0.05% enteric-coated sustained-release BA (ES-B) or 0.10% uncoated BA (NC-B)], a total of 6 groups with 6 replicates per group and 15 broilers per replicate. The trial lasted for 42 days. The results showed as follows: 1) dietary BA had a tendency to increase body weight (BW) at 42 days of age and body weight gain (BWG) from 1 to 42 days of age of broilers (P=0.058, P=0.057). Coccidia infection significantly decreased BW (except for that at 14 days of age), BWG and average feed intake (AFI) (except for those from 1 to 14 days of age) (P<0.05), and significantly increased the ratio of feed to gain (F/G) of broilers from 15 to 21 days of age (P<0.05), and decline range of growth performance in N-B group was greater than that in ES-B and NC-B groups. 2) Dietary BA significantly increased serum immunoglobulin M (IgM) content and thymus index at 21 days of age and serum immunoglobulin A (IgA) content at 42 days of age, and had a tendency to decrease serum nitric oxide (NO) content of broilers at 42 days of age (P=0.068). Coccidia infection significantly increased serum albumin (ALB) content at 42 days of age, and significantly decreased serum alkaline phosphatase (ALP) activity at 21 days of age and serum IgM content of broilers 42 days of age (P<0.05). Dietary BA and coccidia infection had significant interaction on serum IgM content of broilers at 21 days of age (P<0.05), it is showed as under the condition of coccidia infection, dietary BA increased serum IgM content, and ES-B group had the best effects. 3) Dietary BA significantly increased serum glutathione peroxidase (GSH-Px) activity and significantly decreased serum malondialdehyde (MDA) content of broilers at 21 days of age (P<0.05). Dietary BA and coccidia infection had significant interaction on serum MDA content and GSH-Px activity of broilers at 42 days of age (P<0.05), it is showed as under the conditions of coccidia infection, dietary BA increased serum GSH-Px activity and decreased MDA content (P<0.05), and NC-B group had the best effects. In conclusion, dietary BA can improve growth performance of broilers; under the conditions of coccidia infection, dietary BA can alleviate the degradation of growth performance caused by coccidia infection by improving health and immune function, and 0.05% ES-B has better improvement effects.

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