动物营养学报 >

2021 , Vol. 33 >Issue 3: 1708 - 1715

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.03.051

实验方法与实验动物 EXPERIMENTAL METHOD AND ANIMAL

响应面法优化菜籽粕的酶解条件

  • 朱晓峰 ,
  • 张桢 ,
  • 丁立人 ,
  • 杭苏琴
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  • 1. 南京农业大学动物科技学院, 国家动物消化道国际联合中心, 南京 210095;
    2. 南京农业大学动物科技学院, 动物科学类国家级试验教学示范中心, 南京 210095
朱晓峰(1994-),男,贵州黎平人,硕士研究生,从事发酵饲料研究。E-mail:2017105008@njau.edu.cn

收稿日期: 2020-08-14

  网络出版日期: 2021-03-18

基金资助

农业部公益性行业专项（201403047）

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Optimization of Enzymatic Hydrolysis Conditions of Rapeseed Meal by Response Surface Method

  • ZHU Xiaofeng ,
  • ZHANG Zhen ,
  • DING Liren ,
  • HANG Suqin
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  • 1. National Joint Research Center for Animal Digestive Tract Nutrition, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2020-08-14

  Online published: 2021-03-18

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摘要

菜籽粕因粗蛋白质含量（32%~40%）较高可作为动物潜在饲料来源，但由于纤维含量较高，限制了其在猪和禽饲粮中的应用。本试验采用响应面法对复合纤维降解酶（纤维素酶+果胶酶）酶解菜籽粕的条件进行优化，以期提高菜籽粕的还原糖产量、降低其纤维含量。方法：1）通过响应面试验优化酶解条件——pH（3.2~4.8）、温度（45~55℃）、酶解时间（18~30 h）和底物浓度（即菜籽粕浓度，100~200 g/L）；2）以优化前的酶解条件作为对照（未优化组），在最优酶解条件下（优化组）酶解菜籽粕，探究优化的效果。结果：1）响应面试验结果表明，pH、温度和酶解时间对还原糖转化率有极显著影响（P<0.01），pH与温度、温度与时间对还原糖转化率的影响存在显著的交互作用（P<0.05）；2）与对照组（pH 4.8、温度50℃、酶解时间24 h、底物浓度100 g/L）相比，优化组（pH 4.0、温度50℃、酶解时间28 h、底物浓度200 g/L）的还原糖转化率显著升高（P<0.05）；此外，优化组的中性洗涤纤维和酸性洗涤纤维含量显著低于对照组（P<0.05）。结论：1）利用响应面法优化酶解条件后可有效提高菜籽粕产还原糖的效率，降低其纤维含量。2）优化后的酶解条件为pH 4.0、温度50℃、酶解时间28 h、底物浓度200 g/L。

关键词： 响应面法; 纤维素酶; 果胶酶; 酶解条件; 菜籽粕; 还原糖; 纤维含量

本文引用格式

朱晓峰 , 张桢 , 丁立人 , 杭苏琴 . 响应面法优化菜籽粕的酶解条件[J]. 动物营养学报, 2021 , 33(3) : 1708 -1715 . DOI: 10.3969/j.issn.1006-267x.2021.03.051

Abstract

Rapeseed meal (RSM) is a potential source for animal feed due to its relatively high crude protein content (32% to 40%). However, the high fiber content in RSM has limited its application in pig and poultry diets. The aim of this study was to optimized the conditions for enzymatic hydrolysis of RSM by complex fiber-degrading enzyme (cellulase+pectinase) through the response surface method to obtain a higher yield of reducing sugar and lower fiber content. Method: 1) the enzymatic hydrolysis conditions of pH (3.2 to 4.8), temperature (45 to 55 ℃), enzymatic time (18 to 24 h) and substrate concentration (RSM concentration, 100 to 200 g/L) were optimized by response surface experiment; 2) RSM was enzymatic hydrolyzed under the optimal conditions (optimum group), and the enzymatic hydrolysis conditions before optimization were used as a control (control group) to investigate the optimization effect. Results: 1) response surface experiment results showed that pH, temperature and enzymatic time had extremely significant effects on reducing sugar conversion ratio (P<0.01). The pH×temperature and temperature×enzymatic time had significant interactions on reducing sugar conversion ratio (P<0.05). 2) Compared with the control group (4.8 for pH, 50 ℃ for temperature, 24 h for enzymatic time, and 100 g/L for substrate concentration), the reducing sugar conversion ratio in the optimum group (4.0 for pH, 50 ℃ for temperature, 28 h for enzymatic time, and 200 g/L for substrate concentration) was significantly increased (P<0.05), and the contents of neutral detergent fiber and acid detergent fiber were significantly decreased (P<0.05). Conclusion: 1) the enzymatic hydrolysis conditions optimized by response surface method can effectively increase the reducing sugar production and reduce the fiber content of RSM; 2) the optimized conditions are 4.0 for pH, 50 ℃ for temperature, 28 h for enzymatic time, and 200 g/L for substrate concentration.

Key words： response surface method; cellulase; pectinase; enzymatic hydrolysis conditions; rapeseed meal; reducing sugar; fiber content

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