猪营养与饲料 SWINE NUTRITION AND FEED

钙、磷对免疫抑制仔猪生长性能、血液生理生化指标及矿物元素代谢的影响

  • 李净 ,
  • 倪姮佳 ,
  • 黄勇刚 ,
  • 李梦 ,
  • 谭碧娥
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  • 1. 湖南农业大学动物科学技术学院, 动物营养基因组与种质创新研究中心, 长沙 410128;
    2. 中国科学院亚热带农业生态研究所, 动物营养生理与代谢过程湖南省重点实验室, 长沙 410125
李净(1997-),女,湖南醴陵人,硕士研究生,从事动物营养与饲料资源开发与利用研究。E-mail:1543826756@qq.com

收稿日期: 2020-09-18

  网络出版日期: 2021-04-15

基金资助

湖南省重点实验室开放研究基金项目(ISA2020211);广东省基础与应用基础研究基金项目(2019B1515210022)

Effects of Calcium and Phosphorus on Growth Performance, Blood Physiological-Biochemical Indices and Mineral Element Metabolism of Immunosuppressed Piglets

  • LI Jing ,
  • NI Hengjia ,
  • HUANG Yonggang ,
  • LI Meng ,
  • TAN Bi'e
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  • 1. Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received date: 2020-09-18

  Online published: 2021-04-15

Supported by

 

摘要

本试验旨在研究钙、磷对免疫抑制仔猪生长性能、血液生理生化指标及矿物元素代谢的影响。选取18头22日龄"杜长大"三元杂交断奶仔猪(公母各占1/2),随机分为3组,每组6个重复,每个重复1头猪。其中,对照组和模型组饲喂基础饲粮,试验组饲喂含1.5倍NRC(2012)钙、磷标准的饲粮。试验第23天,模型组和试验组仔猪腹腔注射18 mg/kg BW伊立替康(CPT),连续注射4 d,用于建立免疫抑制仔猪模型,对照组注射等量生理盐水。试验期为28 d。结果表明:1)与对照组相比,腹腔注射CPT显著降低了仔猪的平均日增重(P<0.05),显著提高了料重比和腹泻指数(P<0.05),饲粮补充钙、磷可显著缓解免疫抑制仔猪的腹泻(P<0.05),有助于改善仔猪的生长性能。2)与对照组相比,免疫抑制仔猪血液中白细胞、中性粒细胞和单核细胞等免疫细胞的数量显著降低(P<0.05),饲粮补充钙、磷可缓解免疫抑制对血液中单核细胞数量的影响。3)与对照组相比,免疫抑制仔猪血清中铜的含量显著升高(P<0.05),结肠食糜中铜的含量显著降低(P<0.05),提示机体可能通过提高铜利用率实现免疫功能的修复,是对免疫抑制的一种反馈调节。饲粮补充钙、磷对免疫抑制仔猪的微量元素代谢无显著影响(P>0.05)。综上所述,饲粮补充1.5倍NRC(2012)标准的钙、磷可有效缓解CPT所致免疫抑制仔猪的腹泻症状,改善仔猪的生长性能,促进免疫功能的恢复。

本文引用格式

李净 , 倪姮佳 , 黄勇刚 , 李梦 , 谭碧娥 . 钙、磷对免疫抑制仔猪生长性能、血液生理生化指标及矿物元素代谢的影响[J]. 动物营养学报, 2021 , 33(4) : 1954 -1963 . DOI: 10.3969/j.issn.1006-267x.2021.04.016

Abstract

The aim of this study was to investigate the effects of calcium and phosphorus on growth performance, blood physiological-biochemical indices and mineral element metabolism of immunosuppressed piglets. Eighteen Duroc×Landrace×Yorkshire weaned piglets at 22 days of age were selected and randomly divided into three groups, each group consisting of six replicates with one piglet per pen. The control group and model group were fed a basal diet, while the experimental group was fed a diet containing a higher level of calcium and phosphorus[1.5 times NRC (2012) standard]. On the 23rd day, piglets in the model group and the experimental group were injected intraperitoneally with 18 mg/kg BW irinotecan (CPT) for 4 days, which was used to establish the immunosuppressed piglet model. While piglets in the control group was injected with the same amount of normal saline. The experiment lasted for 28 days. The results showed as follows:1) compared with the control group, the intraperitoneal injection of CPT significantly reduced the average daily weight gain of piglets (P<0.05), and significantly increased the feed to gain ratio and the diarrhea index (P<0.05); dietary calcium and phosphorus significantly relieved the diarrhea in immunosuppressed piglets (P<0.05), which was helpful in the improvement of the growth performance. 2) The immune cells such as leukocytes, neutrophils and monocytes in the blood of immunosuppressed piglets were significantly reduced compared with the control group (P<0.05), dietary calcium and phosphorus relieve the impact on the monocytes. 3) Compared with the control group, the copper content in serum increased significantly (P<0.05), and the copper content in colonic digesta decreased significantly in immunosuppressed piglets (P<0.05). It suggested that the copper utilization improvement might facilitate the repair of immune function, which was a positive feedback on the regulation of immunosuppression. Dietary calcium and phosphorus had no significant effect on the metabolism of metal trace elements in immunosuppressed piglets (P>0.05). In conclusion, dietary 1.5 times NRC (2012) calcium and phosphorus can effectively relieve diarrhea symptoms, improve the growth performance, and promote the recovery of immune function in immunosuppressed piglets caused by CPT.

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