动物营养学报 >

2021 , Vol. 33 >Issue 4: 2044 - 2054

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.024

禽营养与饲料 POULTRY NUTRITION AND FEED

甜菜碱对脂多糖刺激仔鹅生长性能、器官指数、血清生化指标及脾脏炎性因子表达的影响

  • 阳金金 ,
  • 杨芷 ,
  • 杨雨 ,
  • 梁静茹 ,
  • 肖霞 ,
  • 杨海明 ,
  • 王志跃
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  • 1. 扬州大学动物科学与技术学院, 扬州 225009;
    2. 扬州大学农业科技发展研究院, 扬州 225009
阳金金(1994-),女,安徽安庆人,硕士研究生,从事家禽生产研究。E-mail:1509041578@qq.com

收稿日期: 2020-08-31

  网络出版日期: 2021-04-15

基金资助

国家现代农业产业技术体系（CARS-42-I1）；江苏省自然科学基金青年基金项目（BK20190897）；扬州市自然科学基金青年基金项目（YZ2019080）

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Effects of Betaine on Growth Performance, Organ Indices, Serum Biochemical Parameters and Spleen Inflammatory Factor mRNA Expression of Geese Challenged by Lipopolysaccharide

  • YANG Jinjin ,
  • YANG Zhi ,
  • YANG Yu ,
  • LING Jingru ,
  • XIAO Xia ,
  • YANG Haiming ,
  • WANG Zhiyue
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  • 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China

Received date: 2020-08-31

  Online published: 2021-04-15

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摘要

本试验旨在研究饲粮中添加甜菜碱（Bet）对脂多糖（LPS）刺激仔鹅生长性能、器官指数、血清生化指标及脾脏炎性因子表达的影响。试验采用2×2双因子随机设计，主因子分别为LPS（腹腔注射LPS或生理盐水）和甜菜碱（饲粮中不添加或添加0.06%甜菜碱）。试验选取15日龄体重相近且健康的江南白鹅公鹅168只，随机分为4个组：Ctrl组（对照组，不添加甜菜碱+腹腔注射1.5 mg/kg BW生理盐水）、Bet组（添加0.06%甜菜碱+腹腔注射1.5 mg/kg BW生理盐水）、LPS组（饲粮中不添加甜菜碱+腹腔注射1.5 mg/kg BW LPS）、Bet+LPS组（饲粮中添加0.06%甜菜碱+腹腔注射1.5 mg/kg BW LPS），每组6个重复，每个重复7只鹅。分别于16、18、20日龄早晨进行注射，21和28日龄称重、屠宰采样。试验分为应激期（16~21日龄）和恢复期（22~28日龄）。结果表明：1）在应激期，LPS刺激显著降低仔鹅21日龄体重、平均日采食量（ADFI）和平均日增重（ADG）（P≤0.05），显著提高料重比（F/G）（P≤0.05）；添加甜菜碱具有提高仔鹅ADFI（P=0.080）和21日龄体重（P=0.090）的趋势；LPS刺激和添加甜菜碱对仔鹅ADFI有显著互作效应（P≤0.05），对21日龄体重有互作效应趋势（P=0.075）。在恢复期，LPS刺激显著降低仔鹅28日龄体重、ADFI和F/G（P≤0.05），对ADG无显著影响（P>0.05）；添加甜菜碱对仔鹅生长性能无显著影响（P>0.05）。2）21日龄时，LPS刺激显著降低仔鹅胸腺指数（P≤0.05），而添加甜菜碱显著提高了仔鹅胸腺指数（P≤0.05）。3）21日龄时，LPS刺激显著降低仔鹅血清白蛋白含量和白蛋白/球蛋白（P≤0.05），显著提高血清球蛋白含量（P≤0.05）；LPS刺激和添加甜菜碱对仔鹅血清白蛋白含量有显著互作效应（P≤0.05）。4）21日龄时，LPS刺激显著提高仔鹅脾脏白细胞介素-1β（IL-1β）mRNA相对表达量（P≤0.05），而添加甜菜碱显著降低脾脏IL-1β mRNA相对表达量（P≤0.05）。28日龄时，LPS刺激和添加甜菜碱对仔鹅脾脏炎症因子mRNA相对表达量均无显著影响（P>0.05）。综上所述，LPS刺激降低仔鹅生长性能和免疫性能；而饲粮中添加0.06%甜菜碱可以改善仔鹅生长性能，提高胸腺指数，降低脾脏促炎因子IL-1β mRNA相对表达量，从而在一定程度上缓解LPS刺激对仔鹅的负面影响。甜菜碱对LPS刺激应激期的缓解作用比恢复期更为明显。

关键词： 甜菜碱; 仔鹅; 脂多糖; 生长性能; 炎性因子

本文引用格式

阳金金 , 杨芷 , 杨雨 , 梁静茹 , 肖霞 , 杨海明 , 王志跃 . 甜菜碱对脂多糖刺激仔鹅生长性能、器官指数、血清生化指标及脾脏炎性因子表达的影响[J]. 动物营养学报, 2021 , 33(4) : 2044 -2054 . DOI: 10.3969/j.issn.1006-267x.2021.04.024

Abstract

The present study aimed to investigate the effects of dietary betaine (Bet) on growth performance, organ indices, serum biochemical parameters and spleen inflammatory factor expression of geese challenged by lipopolysaccharide (LPS). The experiment was designed as a 2×2 double-factor trial design and the principal factors were LPS (intraperitoneal injection of LPS or physiological saline) and Bet (dietary no added or added 0.06% Bet). A total of 168 healthy fifteen-day-old Jiangnan white male geese with similar body weight were selected and randomly divided into 4 groups:Ctrl group (control group, no added Bet+intraperitoneal injection 1.5 mg/kg BW physiological saline), Bet group (added 0.06% Bet+intraperitoneal injection 1.5 mg/kg BW physiological saline), LPS group (no added Bet+intraperitoneal injection 1.5 mg/kg BW LPS) and Bet+LPS group (added 0.06% Bet+intraperitoneal injection 1.5 mg/kg BW LPS), with 6 replicates per group and 7 geese per replicate. Geese were injected in the morning at 16, 18 and 20 days of age,respectively. At 21 and 28 days of age, geese weighed and slaughtered. The experiment were divided into stress period (16 to 21 days of age) and recovery period (22 to 28 days of age). The results showed as follows:1) during the stress period, LPS challenge significantly decreased the body weigh at 21 days of age, average daily feed intake (ADFI) and average daily gain (ADG) of geese (P≤0.05), and significantly increased the ratio of feed to gain (F/G) (P≤0.05); adding betaine had a tendency to increase the ADFI (P=0.080) and body weigh at 21 days of age of geese (P=0.090); LPS challenge and adding betaine had significant interaction on ADFI of geese (P≤0.05), and had a tendency of significant interaction on the body weigh at 21 days of age (P=0.075). During the recovery period, LPS challenge significantly decreased the body weigh at 28 days of age, ADFI and F/G of geese (P≤0.05), and had no significant effect on ADG (P>0.05); adding betaine had no significant effect on the growth performance of geese (P>0.05). 2) At 21 days of age, LPS challenge significantly decrease the thymus index of geese (P≤0.05), while adding betaine significantly increased the thymus index of geese (P≤0.05). 3) At 21 days of age, LPS challenge significantly decrease the serum albumin content and albumin/globulin (P≤0.05), and significantly increased the serum globulin content of geese(P≤0.05); LPS challenge and adding betaine had significant interaction on serum albumin content of geese (P≤0.05). 4) At 21 days of age, LPS challenge significantly increased the mRNA relative expression of interleukin-1β (IL-1β) in spleen of geese (P≤0.05), and adding betaine significantly decrease the mRNA relative expression of IL-1β in spleen of geese (P≤0.05). At 28 days of age, LPS challenge and adding betaine had no significant effects on mRNA relative expression of inflammatory factors in spleen of geese (P>0.05). In conclusion, LPS challenge reduce the growth performance and immune performance of geese; and dietary added 0.06% betaine can improve the growth performance of geese, increase the thymus index and reduce the mRNA relative expression of inflammatory factor IL-1β in spleen, and partly effectively alleviate negative impact of geese challenged by LPS. Betaine can alleviate LPS stress during the stress period more obviously than recovery period.

Key words： betaine; geese; lipopolysaccharide; growth performance; inflammatory factors

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