动物营养学报 >

2021 , Vol. 33 >Issue 4: 2158 - 2167

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.035

反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

亚急性瘤胃酸中毒对绵羊瘤胃异常代谢产物、瘤胃菌群和血液相关指标的影响

  • 韩郭皓 ,
  • 高晓莎 ,
  • 段晋伟 ,
  • 张慧琴 ,
  • 郑岩 ,
  • 马林峰 ,
  • 何金鑫 ,
  • 霍乃蕊 ,
  • 裴彩霞 ,
  • 古少鹏
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  • 1. 山西农业大学动物医学学院, 太谷 030801;
    2. 山西农业大学动物科学学院, 太谷 030801
韩郭皓(1997-),男,山西太原人,硕士研究生,从事兽医公共卫生与动物疾病防治研究。E-mail:hanguohao5@163.com

收稿日期: 2020-08-01

  网络出版日期: 2021-04-15

基金资助

山西省重点研发计划项目（201803D221026-3）；山西省"1331工程"重点创新团队建设计划；山西省现代农业产业体系羊体系

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Effects of Subacute Ruminal Acidosis on Rumen Abnormal Metabolites, Rumen Bacterial Flora and Blood Related Indexes of Sheep

  • HAN Guohao ,
  • GAO Xiaosha ,
  • DUAN Jinwei ,
  • ZHANG Huiqin ,
  • ZHENG Yan ,
  • MA Linfeng ,
  • HE Jinxing ,
  • HUO Nairui ,
  • PEI Caixia ,
  • GU Shaopeng
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  • 1. College of Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
    2. College of Animal Science, Shanxi Agricultural University, Taigu 030801, China

Received date: 2020-08-01

  Online published: 2021-04-15

Supported by

 

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摘要

本试验通过逐步递增饲粮精粗比诱导绵羊发生亚急性瘤胃酸中毒（SARA），探究其对瘤胃异常代谢产物、瘤胃菌群、血气和血清生化指标的影响，为防治绵羊SARA提供理论依据。选取50只2月龄同期断奶健康的杜泊羊（♂）×湖羊（♀）F1代，随机分为2组。对照组（CK组，n=15）饲喂精粗比为50：50的基础饲粮；SARA诱导组通过饲粮精粗比递增诱导绵羊发生SARA，饲粮精粗比依次为50：50、70：30、80：20、90：10和100：0，各精粗比饲粮均饲喂7 d，瘤胃液pH在5.2~5.6持续3 h以上的绵羊构成SARA模型组。分别于各精粗比饲粮第7天晨饲后1、2、3、4、5、6和8 h采集瘤胃液，测定瘤胃异常代谢产物含量和瘤胃菌群结构。SARA诱导成功后，采集CK组和SARA模型组颈静脉血液，测定血气和血清生化指标。结果显示：1）在饲粮精粗比为100：0时，SARA诱导组中有27只绵羊瘤胃液pH在5.2~5.6持续3 h以上，部分羊腹泻，瘤胃液偏黄恶臭黏稠，符合SARA标准，构成SARA模型组。2）SARA模型组Chao1指数显著低于CK组（P<0.05），瘤胃菌群结构发生变化，乳酸产生菌相对丰度增加，瘤胃菌群碳水化合物代谢相关基因富集程度增加。3）SARA模型组瘤胃液和血液中乳酸、组胺和内毒素含量均显著高于CK组（P<0.05）。4）SARA模型组血液二氧化碳总量、实际碳酸氢盐和全血碱剩余含量显著低于CK组（P<0.05）。5）SARA模型组血清谷丙转氨酶活性显著低于CK组（P<0.05），血清生化指标处于正常范围。综上所述，绵羊SARA发生过程中，瘤胃菌群丰富度降低，菌群结构改变，乳酸产生菌相对丰度增加，血液和瘤胃液乳酸、内毒素和组胺含量升高，机体发生代谢性酸中毒。

关键词： 亚急性瘤胃酸中毒; 绵羊; 瘤胃菌群; 代谢性酸中毒; 炎症反应

本文引用格式

韩郭皓 , 高晓莎 , 段晋伟 , 张慧琴 , 郑岩 , 马林峰 , 何金鑫 , 霍乃蕊 , 裴彩霞 , 古少鹏 . 亚急性瘤胃酸中毒对绵羊瘤胃异常代谢产物、瘤胃菌群和血液相关指标的影响[J]. 动物营养学报, 2021 , 33(4) : 2158 -2167 . DOI: 10.3969/j.issn.1006-267x.2021.04.035

Abstract

In order to provide a theoretical basis for prevention and cure of sheep subacute ruminal acidosis (SARA), the effects of rumen abnormal metabolites, rumen bacterial flora, blood gas and serum biochemical indexes were explored after sheep SARA-induced by gradually increasing the concentrate to forage ratio. Fifty 2-month-old weaned Dorper sheep (♂)×Hu sheep (♀) F1 generations were randomly divided into two groups. The control group (CK group, n=15) was fed a basal diet with concentrate to forage ratio of 50:50, while the SARA induction groups (n=35) were fed diets with gradually increased the concentrate to forage ratios of 50:50, 70:30, 80:20, 90:10 and 100:0 for 7 d at each ratio. Sheep in the SARA induction groups whose rumen fluid pH was between 5.2 and 5.6 for more than 3 h constituted the SARA model group. The rumen fluid was collected at 1, 2, 3, 4, 5, 6 and 8 h after morning feeding on the 7th day of each ratio, and the rumen abnormal metabolites contents and bacterial flora structure were measured. Then, the jugular vein blood was collected from the CK group and the SARA model group, and the blood gas and serum biochemical indexes were measured. The results showed as follows:1) when the concentrate to forage ratio was 100:0, rumen pH of 27 sheep in SARA induction group was 5.2 to 5.6 and lasted for more than 3 h, which were made into the SARA model group. Furthermore, some sheep had diarrhoea, and the rumen fluid was yellowish, smelly and sticky. 2) The Chao1 index of SARA model group was significantly lower than that of CK group (P<0.05), the rumen bacterial flora structure changed, the relative abundance of lactic acid-producing bacteria increased, and the genes related to carbohydrate metabolism in the rumen bacterial flora increased. 3) The contents of lactic acid, histamine and endotoxin in the rumen fluid and blood of SARA model group were significantly higher than those of CK group (P<0.05). 4) The contents of total amount of carbon dioxide, actual bicarbonate and total blood alkali in blood of SARA model group were significantly lower than those of CK group (P<0.05). 5) The serum alanine aminotransferase activity of SARA model group was significantly lower than that of CK group (P<0.05), and both serum biochemical indexes were in the normal range. In summary, the richness of rumen bacterial flora decreases, the bacterial flora structure changes and the lactic acid-producing bacteria relative abundance increases in SARA sheep, the contents of lactic acid, endotoxin and histamine in blood and rumen fluid increase, and metabolic acidosis occurs.

Key words： subacute ruminal acidosis; sheep; rumen bacterial flora; metabolic acidosis; inflammatory response

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