动物营养学报 >

2021 , Vol. 33 >Issue 4: 2311 - 2320

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.04.049

研究简报 SHORT COMMUNICATION

复方银杏叶对免疫应激仔猪生长性能和血清代谢组学特征的影响

  • 李焰 ,
  • 岳稳 ,
  • 李婷 ,
  • 潘佳佳 ,
  • 邱龙新 ,
  • 黄杰 ,
  • 李状伟
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  • 1. 龙岩学院生命科学学院, 龙岩 364000;
    2. 福建省生猪疫病防控工程技术研究中心, 龙岩 364000;
    3. 福建省家畜传染病防治与生物技术重点实验室, 龙岩 364000;
    4. 福建农林大学动物科学学院(蜂学学院), 福州 350000
李焰(1968-),女,四川安县人,教授,硕士,主要从事畜禽营养与免疫研究。E-mail:529783204@qq.com

收稿日期: 2020-05-15

  网络出版日期: 2021-04-15

基金资助

国家自然科学基金面上项目（31470129）；中央引导地方科技发展专项（2019L3011）；龙岩市科技局农业类重点项目（2017LY27）

收起

Effect of Ginkgo biloba Compound on Growth Performance and Serum Metabolomics Characteristics of Immune Stressed Piglets

  • LI Yan ,
  • YUE Wen ,
  • LI Ting ,
  • PAN Jiajia ,
  • QIU Longxin ,
  • HUANG Jie ,
  • LI Zhuangwei
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  • 1. College of Life Science, Longyan University, Longyan 364000, China;
    2. Fujian Provincial Engineering & Technology Research Center for the Prevention and Control of Swine Diseases, Longyan 364000, China;
    3. Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364000, China;
    4. College of Animal Sciences(College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350000, China

Received date: 2020-05-15

  Online published: 2021-04-15

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摘要

本试验旨在研究复方银杏叶对脂多糖（LPS）诱导的免疫应激仔猪生长性能及血清生化指标的影响，并采用代谢组学技术研究免疫应激仔猪的血清代谢组学特征。选择"杜×长×大"杂交仔猪36头，随机分为对照组（每天灌服110 mL生理盐水）、LPS应激组（每天灌服110 mL生理盐水）和LPS+复方银杏叶组（每天灌服110 mL复方银杏叶制剂），每组4个重复，每个重复3头，3个组均饲喂同一基础饲粮，试验期27 d。试验第23天，LPS应激组和LPS+复方银杏叶组仔猪腹膜注射LPS，建立免疫应激模型；对照组仔猪腹膜注射等量的生理盐水。结果显示：1）在试验第24~27天，LPS+复方银杏叶组的料重比与LPS应激组相比降低了35.77%（P<0.05），与对照组相比差异不显著（P>0.05）；2）与LPS应激组相比，复方银杏叶显著缓解了LPS刺激引起的尿素氮、肌酐含量和γ-谷氨酰转移酶活性的升高（P<0.05），以及LPS刺激引起的胰高血糖素样肽-2含量的降低（P<0.05）；3）差异代谢物分析显示，与LPS应激组相比，LPS+复方银杏叶组血清N6-甲基-L-赖氨酸和L-谷氨酸含量显著上升（P<0.05），而血清谷氨酰胺、羟（基）脯氨酸和L-焦谷氨酸含量显著下降（P<0.05）。由此可见，复方银杏叶可显著缓解LPS应激导致的仔猪血清生化指标的改变，减轻LPS应激导致的肝脏损伤，在一定程度上改善仔猪对营养物质的消化和吸收，提高机体对含氮物质的利用效率以及肠黏膜屏障功能，从而保证仔猪的正常生长发育；复方银杏叶可能通过调控D-谷氨酰胺和D-谷氨酸代谢以及丙氨酸、天冬氨酸和谷氨酸代谢通路，进而改善LPS诱导的仔猪免疫应激。

关键词： 复方银杏叶; 仔猪; 免疫应激; 脂多糖; 代谢组学

本文引用格式

李焰 , 岳稳 , 李婷 , 潘佳佳 , 邱龙新 , 黄杰 , 李状伟 . 复方银杏叶对免疫应激仔猪生长性能和血清代谢组学特征的影响[J]. 动物营养学报, 2021 , 33(4) : 2311 -2320 . DOI: 10.3969/j.issn.1006-267x.2021.04.049

Abstract

The aim of this experiment was conducted to study the effects of Ginkgo biloba compound on growth performance and serum biochemical indexes of immune stressed piglets induced by lipopolysaccharide (LPS), and to investigate the serum metabolomics characteristics of immune stressed piglets via metabolomics technology. Thirty-six "Duroc×Landrace×Large White" piglets were randomly allocated into control group (gavaged with 110 mL normal saline per day), LPS-stressed group (gavaged with 110 mL normal saline per day) and LPS+Ginkgo biloba compound group (gavaged with 110 mL Ginkgo biloba compound per day). Each group had 4 replicates and each replicate had 3 piglets, all groups were fed the same basal diet. The experiment lasted for 27 days. At day 23 of experiment, LPS was injected into the peritoneum of piglets in LPS-stressed group and LPS+Ginkgo biloba compound group to establish the immune stress model; the piglets in the control group were injected with the same dose of normal saline. The results showed as follows:1) during day 24 to 27 of experiment, the feed/gain (F/G) of LPS+Ginkgo biloba compound group was 35.77% lower than that of LPS-stressed group (P<0.05), and the difference was not significant when compared with the control group (P>0.05); 2) compared with the LPS-stressed group, Ginkgo biloba compound significantly alleviated the LPS-induced increase of the contents of urea nitrogen (UN), creatinine (CREA) and the activity of γ-glutamyltransferase (γ-GGT) (P<0.05), as well as the decrease of the content of glucagon-like peptide 2 (GLP2) (P<0.05); 3) through differential metabolite analysis, compared with the LPS-stressed group, the serum contents of N6-methyl-L-lysine and L-glutamate in the LPS+Ginkgo biloba compound group were significantly increased (P<0.05), while the contents of L-glutamine, hydroxyproline and L-pyroglutamic acid were significantly decreased (P<0.05). In conclusion, Ginkgo biloba compound can significantly alter the changes of serum biochemical parameters of piglets caused by LPS, alleviate the LPS-induced liver damage to a certain extent, and improve the digestion and absorption of nutrients and the use efficiency of nitrogenous substance in piglets, as well as improve the intestinal mucosal barrier function, thus ensure the requirement for normal growth and development of piglets. Ginkgo biloba compound may improve the immune stress of LPS-challenged piglets by regulating D-glutamine and D-glutamate metabolism pathway as well as alanine, aspartic acid and glutamate metabolism pathway.

Key words： Ginkgo biloba compound; piglets; immune stress; lipopolysaccharide; metabolomics

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