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妊娠中后期饲粮添加氮氨甲酰谷氨酸对新生子代滩羊生长发育、脂肪沉积相关基因表达以及母羊血清生化指标的影响

  • 钟锐 ,
  • 李荫柱 ,
  • 刘博 ,
  • 牛文智 ,
  • 张桂杰
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  • 1. 宁夏大学动物科学系, 银川 750021;
    2. 吴忠市红寺堡区天源良种羊繁育养殖有限公司, 吴忠 751900
钟锐(1995-),男,陕西榆林人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:962267798@qq.com

收稿日期: 2020-09-02

  网络出版日期: 2021-04-15

基金资助

国家自然科学基金——妊娠中后期氮氨甲酰谷氨酸对新生子代滩羊脂肪沉积的作用及营养调控(31702134);教育部霍英东青年教师基金——妊娠中后期氮氨甲酰谷氨酸对子代滩羊脂肪分配的程序化作用及营养调控(161106);宁夏高等学校一流学科建设(草学学科)资助项目(NXYLXK2017A01);农牧交错带牛羊牧繁农育关键技术集成示范项目(16200158-08)

Effects of Dietary N-Carbamyl Glutamate in Middle and Late Gestation Period on Growth and Development, Expression of Fat Deposition Related Genes of Newborn Offspring Tan Sheep and Serum Biochemical Parameters of Ewes

  • ZHONG Rui ,
  • LI Yinzhu ,
  • LIU Bo ,
  • NIU Wenzhi ,
  • ZHANG Guijie
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  • 1. Department of Animal Science, Ningxia University, Yinchuan 750021, China;
    2. Hongsipu District Tianyuan Well-Bred Sheep Breeding Co., Ltd., Wuzhong 751900, China

Received date: 2020-09-02

  Online published: 2021-04-15

Supported by

 

摘要

本试验旨在探讨妊娠中后期饲粮添加氮氨甲酰谷氨酸(NCG)对新生子代滩羊生长发育、脂肪沉积相关基因表达以及母羊血清生化指标的影响。选用胎次(第2胎次)、年龄(3岁龄)和体重[(47.75±1.20)kg]相近的滩羊母羊80只,同期发情后人工授精,成功受孕63只,选择60只妊娠母羊进行试验。妊娠70 d后将母羊按体重随机分为2组,每组5个重复,每个重复6只羊。对照组母羊饲喂基础饲粮,试验组在基础饲粮中添加0.10% NCG。试验直至分娩结束,各组母羊均产羔羊1只。结果表明:1)与对照组相比,试验组新生子代滩羊初生重显著上升了6.1%(P<0.05),体长和体高有上升趋势(0.05≤P<0.10)。2)与对照组相比,试验组新生子代滩羊肾周脂肪中脂蛋白脂酶(LPL)mRNA相对表达水平显著提高了18.6%(P<0.05),而肾周脂肪中葡萄糖转运蛋白4(GLUT4)mRNA相对表达水平显著下降了13.3%(P<0.05);新生子代滩羊肠脂、肾脂和尾脂中硬脂酰辅酶A去饱和酶(SCD)mRNA相对表达水平有上升趋势(0.05≤P<0.10)。3)与对照组相比,试验组母羊血清总蛋白和球蛋白含量有上升趋势(0.05≤P<0.10),血清胆固醇、葡萄糖和尿素氮含量有下降趋势(0.05≤P<0.10)。综上所述,妊娠中后期滩羊饲粮中添加0.10% NCG能显著提高新生子代滩羊初生重,同时改善新生子代滩羊脂肪沉积相关基因LPLSCDGLUT4的表达。

本文引用格式

钟锐 , 李荫柱 , 刘博 , 牛文智 , 张桂杰 . 妊娠中后期饲粮添加氮氨甲酰谷氨酸对新生子代滩羊生长发育、脂肪沉积相关基因表达以及母羊血清生化指标的影响[J]. 动物营养学报, 2021 , 33(4) : 2355 -2364 . DOI: 10.3969/j.issn.1006-267x.2021.04.053

Abstract

The aim of this study was to investigate the effects of dietary N-carbamyl glutamate (NCG) in middle and late gestation period on growth and development, expression of fat deposition related genes of newborn offspring Tan sheep and serum biochemical parameters of ewes. Eighty Tan sheep ewes with similar parity (the second parity), age (three years old) and body weight[(47.75±1.20) kg] were selected for artificial insemination after synchronous estrus. Sixty-three ewes were successfully conceived, and sixty of them were selected for the experiment. After seventy days of gestation, the ewes were randomly divided into two groups according to their body weight, with five replicates in each group and six ewes in each replicate. Ewes in the control group were fed a basic diet, and others in the experimental group were fed the basic diet supplemented with 0.10% NCG. Until the end of parturition, all ewes in each group produced 1 lamb. The results showed as follows:1) compared with the control group, the birth weight of newborn offspring Tan sheep in the experimental group increased significantly by 6.1% (P<0.05), and the body length and body height had an upward tendency (0.05≤P<0.10). 2) Compared with the control group, the mRNA relative expression levels of lipoprotein lipase (LPL) in perirenal fat of newborn offspring Tan sheep in the experimental group was significantly increased by 18.6% (P<0.05), while the mRNA relative expression level of glucose transporter 4 (GLUT4) in perirenal fat was significantly decreased by 13.3% (P<0.05); and the mRNA relative expression level of stearoyl coenzyme A desaturase (SCD) in enterolactone, pararenal fat and tail fat of newborn offspring Tan sheep had an upward tendency (0.05≤P<0.10). 3) Compared with the control group, the contents of total protein and globulin in serum of ewes had an upward tendency (0.05≤P<0.10), and the contents of cholesterol, glucose and urea nitrogen in serum had an downward tendency (0.05≤P<0.10). In conclusion, dietary 0.10% NCG of Tan sheep in middle and late gestation period can increase the birth weight of newborn offspring Tan sheep, and improve the expression of fat deposition related genes in newborn offspring Tan sheep, such as LPL, SCD and GLUT4.

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