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荞麦秸秆饲粮条件下甘露寡糖对滩羊瘤胃菌群结构的影响

  • 马秀花 ,
  • 桂瑞麒 ,
  • 焦娜 ,
  • 杨万宗 ,
  • 李庆敏 ,
  • 周玉香
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  • 宁夏大学农学院, 银川 750021
马秀花(1995-),女,回族,宁夏吴忠人,硕士研究生,研究方向为反刍动物营养。E-mail:2095602677@qq.com

收稿日期: 2020-09-14

  网络出版日期: 2021-04-15

基金资助

农业部公益性行业(农业)项目(201503134)

Effect of Mannan Oligosaccharides on Rumen Bacterial Flora Structure of Tan Sheep under Buckwheat Straw Diet Condition

  • MA Xiuhua ,
  • GUI Ruiqi ,
  • JIAO Na ,
  • YANG Wanzong ,
  • LI Qingmin ,
  • ZHOU Yuxiang
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  • Agricultural College of Ningxia University, Yinchuan 750021, China

Received date: 2020-09-14

  Online published: 2021-04-15

Supported by

 

摘要

本试验旨在利用16S rDNA高通量测序技术探究荞麦秸秆饲粮条件下添加不同水平甘露寡糖(MOS)对滩羊瘤胃菌群结构的影响。选取健康、体重在30.5 kg左右的宁夏断奶滩羊羯羊20只,随机分为4组,每组5只。其中,CK-3组为对照组,饲喂基础饲粮;Y1-3组、Y2-3组、Y3-3组为试验组,分别饲喂在基础饲粮基础上添加1%、2%、3% MOS的试验饲粮。试验期为75 d,其中预试期15 d,正试期60 d。结果显示:1)除Y1-3组、Y2-3组瘤胃液乙酸含量显著高于Y3-3组(P<0.05)外,各瘤胃发酵参数在各组间无显著差异(P>0.05)。2)Alpha多样性分析显示,Y1-3组、Y2-3组、Y3-3组较CK-3组瘤胃菌群多样性增加。聚类分析显示,与CK-3组瘤胃菌群构成相比差异由大到小的试验组依次为Y2-3组、Y1-3组、Y3-3组。3)在门水平上,Y2-3组较其他组极显著提高了厚壁菌门、拟杆菌门的相对丰度(P<0.01),而极显著降低了变形菌门的相对丰度(P<0.01),且浮霉菌门、Kiritimatiellaeota和黏胶球形菌门相对丰度较其他组高。4)在属水平上,相比其他组,添加2% MOS对滩羊瘤胃菌群结构的影响最大,包括赖氨酸芽孢杆菌属、普雷沃氏菌属1、醋杆菌属3种优势菌属在内的9种菌属的相对丰度极显著大于其他组(P<0.01)。总体而言,荞麦秸秆饲粮中添加MOS可以增加滩羊瘤胃菌群多样性,其中2% MOS对滩羊瘤胃菌群结构的影响最大,其有利于增强滩羊对饲粮纤维物质的降解能力。

本文引用格式

马秀花 , 桂瑞麒 , 焦娜 , 杨万宗 , 李庆敏 , 周玉香 . 荞麦秸秆饲粮条件下甘露寡糖对滩羊瘤胃菌群结构的影响[J]. 动物营养学报, 2021 , 33(4) : 2365 -2377 . DOI: 10.3969/j.issn.1006-267x.2021.04.054

Abstract

The purpose of this experiment was to explore the effects of adding different levels of mannose oligosaccharides (MOS) on the rumen bacterial flora structure of Tan sheep under the condition of buckwheat straw diet using 16S rDNA sequencing technology. Twenty Ningxia weaned Tan wethers, healthy and weighing about 30.5 kg, were randomly divided into 4 groups and each group had 5 sheep. The sheep CK-3 group (control group) was fed a basal diet, and the sheep in test groups were fed the basal diet supplemented with was fed with 1% (Y1-3 group), 2% (Y2-3 group) and 3% (Y3-3 group) MOS, respectively. The test period was 75 days, of which the pre-feeding period was 15 days and the normal feeding period was 60 days. The results showed as follows:1) only the acetate content in rumen fluid in Y1-3 group and Y2-3 group was significantly higher than that of Y3-3 group (P<0.05), adding different levels of MOS had no significant effects on the other rumen fermentation parameters (P>0.05). 2) Alpha diversity analysis showed that the diversity of rumen bacterial flora in Y1-3 group, Y2-3 group and Y3-3 group was increased compared with CK-3 group. Cluster analysis showed that, Compared with CK-3 group, the differences in the composition of the rumen bacterial flora in test groups from large to small were Y2-3 group, Y1-3 group, and Y3-3 group. 3) At the phylum level, the Y2-3 group significantly increased the relative abundances of Firmicutes and Bacteroides (P<0.01) and significantly decreased the relative abundance of Proteobacteria compared with other groups (P<0.01), and the relative abundances of Planctomycete, Spirophyta and Glucosphere were also higher than those in other groups. 4) At the genus level, compared with other groups, adding 2% MOS had the greatest impact on the structure of rumen bacterial flora of Tan sheep, the relative abundances of 9 bacteria genera including three dominant bacteria genera of Lysinibacillus, Prevotella_1 and Acetobacter were significantly higher than those in other groups (P<0.01). In general, adding MOS to the buckwheat straw diet can increase the diversity of rumen bacterial flora of Tan sheep. Among them, addition of 2% MOS has the greatest impact on the structure of the rumen bacterial flora of Tan sheep, and it is beneficial to improve the ability of Tan sheep to degrade dietary fiber substances.

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