动物营养学报 >

2021 , Vol. 33 >Issue 5: 2555 - 2567

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.05.016

猪营养与饲料 SWINE NUTRITION AND FEED

壳寡糖对生长育肥猪生长性能、肉品质、抗氧化功能以及免疫功能的影响

  • 苏维发 ,
  • 周洪彬 ,
  • 蒋登湖 ,
  • 刘鑫 ,
  • 汪以真 ,
  • 王新霞 ,
  • 路则庆
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  • 1. 浙江大学动物科学学院, 生物饲料安全与污染防控国家工程试验室, 农业部华东动物营养与饲料重点试验室, 浙江省饲料与动物营养重点试验室, 杭州 310058;
    2. 大连成三畜牧业有限公司, 大连 116000
苏维发(1993-),男,福建屏南人,博士研究生,研究方向为动物营养与饲料科学。E-mail:508364867@qq.com

收稿日期: 2020-10-22

  网络出版日期: 2021-05-14

基金资助

国家生猪产业体系岗位科学家项目（CARS-35）

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Effects of Chitosan Oligosaccharide on Growth Performance, Meat Quality, Antioxidant Capacity and Immune Function of Growing-Finishing Pigs

  • SU Weifa ,
  • ZHOU Hongbin ,
  • JIANG Denghu ,
  • LIU Xin ,
  • WANG Yizhe ,
  • WANG Xinxia ,
  • LU Zeqing
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  • 1. Key Laboratory of Animal Nutrition and Feed Science of Zhejiang Province, Key Laboratory of Animal Nutrition and Feed Science in Eastern China, Ministry of Agriculture, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China;
    2. Dalian Chengsan Animal Husbandry Co., Ltd., Dalian 116000, China

Received date: 2020-10-22

  Online published: 2021-05-14

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摘要

本试验旨在研究壳寡糖（COS）对生长育肥猪生长性能、肉品质、抗氧化功能以及免疫功能的影响。试验选取体重[（37.00±1.55）kg]相近的"杜×长×大"生长猪225头，随机分成3组，每组3个重复，每个重复25头。对照组饲喂基础饲粮，COS组饲喂添加30 mg/kg COS的基础饲粮，低能低蛋白质+COS组饲喂添加30 mg/kg COS的低能低蛋白质饲粮（粗蛋白质含量降低1个百分点，消化能降低0.21 MJ/kg）。试验期98 d。结果表明：1）与对照组相比，COS组生长育肥猪料重比显著降低（P<0.05）。2）与对照组相比，COS组生长育肥猪背最长肌中肌苷酸和部分鲜味氨基酸（谷氨酸、苯丙氨酸和丙氨酸）含量显著提高（P<0.05），低能低蛋白质+COS组生长育肥猪背最长肌中肌苷酸含量同样显著提高（P<0.05）。3）与对照组相比，COS组生长育肥猪血清总抗氧化能力显著提高（P<0.05），血清丙二醛含量显著降低（P<0.05）；同时，COS组生长育肥猪肝脏中相关抗氧化基因——谷胱甘肽过氧化物酶1（GPx1）、谷胱甘肽过氧化物酶4（GPx4）和p53的mRNA相对表达量显著提高（P<0.05）。4）与对照组相比，COS组生长育肥猪血清免疫球蛋白G和免疫球蛋白M含量显著提高（P<0.05），低能低蛋白质+COS组生长育肥猪血清免疫球蛋白M含量也显著提高（P<0.05）；同时，COS组和低能低蛋白质+COS组生长育肥猪血清补体4含量均显著提高（P<0.05）。5）与对照组相比，COS组生长育肥猪十二指肠绒毛高度与隐窝深度的比值显著提高（P<0.05）。综上所述，基础饲粮添加30 mg/kg COS可通过提高机体的免疫功能、抗氧化功能及改善肠道黏膜形态，进而提高生长育肥猪的生长性能，并一定程度上改善猪肉品质。

关键词： 壳寡糖; 生长育肥猪; 生长性能; 肉品质; 抗氧化功能; 免疫功能

本文引用格式

苏维发 , 周洪彬 , 蒋登湖 , 刘鑫 , 汪以真 , 王新霞 , 路则庆 . 壳寡糖对生长育肥猪生长性能、肉品质、抗氧化功能以及免疫功能的影响[J]. 动物营养学报, 2021 , 33(5) : 2555 -2567 . DOI: 10.3969/j.issn.1006-267x.2021.05.016

Abstract

This study was conducted to investigate the effects of chitosan oligosaccharide (COS) on growth performance, meat quality, antioxidant capacity and immune function of growing-finishing pigs. Two hundred and twenty-five Duroc×Landrace×Yorkshire growing pigs with similar body weight of (37.00±1.55) kg were randomly divided into 3 groups with 3 replicates per group and 25 pigs per replicate. Pigs in the control group were fed a basal diet, those in the COS group were fed the basal diet supplemented with 30 mg/kg COS, and those in the low energy and low protein+COS group were fed a low level of dietary energy and protein diet (adjusting the feed formulation to make the basal diet minus 1% crude protein and 0.21 MJ/kg digestible energy) supplemented with 30 mg/kg COS. The trial lasted for 98 days. The results showed as follows:1) compared with the control group, the ratio of feed to gain of growing-finishing pigs in the COS group was significantly reduced (P<0.05). 2) Compared with the control group, the contents of inosinic acid and some umami amino acids (glutamate, phenylalanine and alanine) in longissimus dorsi of growing-finishing pigs in the COS group were significantly increased (P<0.05). 3) Compared with the control group, the serum total antioxidant capacity of growing-finishing pigs in the COS group was significantly increased (P<0.05), and the serum malondialdehyde content was significantly decreased (P<0.05). Meanwhile, the mRNA relative expression levels of antioxidant genes, including glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4) and p53 in liver of growing-finishing pigs in the COS group were significantly increased (P<0.05). 4) Compared with the control group, serum contents of immunoglobulin G and immunoglobulin M of growing-finishing pigs in the COS group were significantly increased (P<0.05), and the serum immunoglobulin M content of growing-finishing pigs in the low energy and low protein+COS group was also significantly increased (P<0.05). Meanwhile, the serum complement 4 content of growing-finishing pigs in the COS group and the low energy and low protein+COS group was significantly increased (P<0.05). 5) Compared with the control group, the villus height to crypt depth ratio in duodenum of growing-finishing pigs in the COS group was significantly increased (P<0.05). In conclusion, the supplementation of 30 mg/kg COS in the basal diet can improve the growth performance and meat quality in a certain extent of growing-finishing pigs by enhancing the immune function, the antioxidant capacity and the intestinal mucosa morphology.

Key words： chitosan oligosaccharide; growing-finishing pigs; growth performance; meat quality; antioxidant capacity; immune function

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