猪营养与饲料 SWINE NUTRITION AND FEED

柑橘提取物对肥育猪肠道抗氧化指标、消化酶活性、氨态氮含量以及粪便氮、磷、臭气含量的影响

  • 崔艺燕 ,
  • 田志梅 ,
  • 邓盾 ,
  • 鲁慧杰 ,
  • 刘志昌 ,
  • 王刚 ,
  • 马现永
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  • 1. 广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业农村部华南动物营养与饲料重点实验室, 广东省畜禽育种与营养研究重点实验室, 广东畜禽肉品质量安全控制与评定工程技术研究中心, 广州 510640;
    2. 岭南现代农业科学与技术广东省实验室茂名分中心, 茂名 525000
崔艺燕(1987-),女,广东佛山人,助理研究员,硕士,从事动物营养与饲料科学、生态养殖与环境控制研究。E-mail:958117076@qq.com

收稿日期: 2020-10-17

  网络出版日期: 2021-05-14

基金资助

饲料产业技术体系创新团队建设项目(2020KJ115);广东省现代农业技术研究体系饲料创新团队(2019KJ115);动物健康养殖国际科技合作示范基地(2019A050505007);清远市启航计划(2018002);广东省农业科学院人才项目(201803)

Effects of Citrus Extract on Intestinal Antioxidant Indices, Digestive Enzyme Activities, Ammonia Nitrogen Contents and Fecal Nitrogen, Phosphorus and Odor Contents of Finishing Pigs

  • CUI Yiyan ,
  • TIAN Zhimei ,
  • DENG Dun ,
  • LU Huijie ,
  • LIU Zhichang ,
  • WANG Gang ,
  • MA Xianyong
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  • 1. Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, The Key Laboratory of Animal Nutrition and Feed Science(South China) of Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming 525000, China

Received date: 2020-10-17

  Online published: 2021-05-14

Supported by

 

摘要

本试验旨在研究柑橘提取物对肥育猪肠道抗氧化指标、消化酶活性、氨态氮含量以及粪便氮、磷、臭气含量的影响。选取108头56日龄、体重(19.73±0.39)kg的"杜×长×大"肥育猪,随机分为3个组,每组6个重复,每个重复6头猪。对照组饲喂基础饲粮,抗生素组在基础饲粮中添加75 g/t金霉素,柑橘提取物组在基础饲粮中添加柑橘提取物(56~112日龄250 mL/t,113~194日龄200 mL/t)。试验期138 d。结果表明:1)与抗生素组相比,柑橘提取物组十二指肠丙二醛含量呈降低趋势(0.05≤P<0.10),空肠丙二醛含量显著降低(P<0.05)。2)与对照组相比,柑橘提取物组十二指肠胃蛋白酶和脂肪酶活性显著提高(P<0.05),淀粉酶活性显著降低(P<0.05)。与对照组和抗生素组相比,柑橘提取物组空肠胃蛋白酶活性显著提高(P<0.05),淀粉酶活性显著降低(P<0.05);与对照组相比,柑橘提取物组空肠胰蛋白酶活性显著提高(P<0.05)。与对照组相比,柑橘提取物组回肠淀粉酶活性显著降低(P<0.05)。3)各组之间肠道氨态氮含量和粪便氮、磷含量无显著差异(P>0.05)。4)各组之间粪便氨气和硫化氢含量无显著差异(P>0.05)。由此可见,柑橘提取物提高了肥育猪肠道蛋白酶和脂肪酶活性,降低了肠道淀粉酶活性,对肠道抗氧化能力及粪便氮、磷和臭气排放无负面影响。

本文引用格式

崔艺燕 , 田志梅 , 邓盾 , 鲁慧杰 , 刘志昌 , 王刚 , 马现永 . 柑橘提取物对肥育猪肠道抗氧化指标、消化酶活性、氨态氮含量以及粪便氮、磷、臭气含量的影响[J]. 动物营养学报, 2021 , 33(5) : 2585 -2594 . DOI: 10.3969/j.issn.1006-267x.2021.05.019

Abstract

The purpose of this experiment was to study the effects of citrus extract on intestinal antioxidant indices, digestive enzyme activities, ammonia nitrogen contents and fecal nitrogen, phosphorus and odor contents of finishing pigs. One hundred and eight 56-day-old "Duroc×Landrace×Large White" finishing pigs with body weight of (19.73±0.39) kg were randomly divided into 3 groups with 6 replicates per group and 6 pigs per replicate. Pigs in the control group were fed a basal diet, pigs in antibiotic group were fed the basal diet supplemented with 75 g/t chlortetracycline, and pigs in citrus extract group were fed the basal diet supplemented with citrus extract (250 mL/t during 56 to 112 days of age and 200 mL/t during 113 to 194 days of age). The experiment lasted for 138 days. The results showed as follows. 1) compared with the antibiotic group, the content of malondialdehyde in duodenum of citrus extract group showed a decreasing tendency (0.05≤P<0.10), and the content of malondialdehyde in jejunum was significantly decreased (P<0.05). 2) Compared with the control group, the activities of pepsin and lipase in duodenum of citrus extract group were significantly increased (P<0.05), and the amylase activity was significantly decreased (P<0.05). Compared with the control group and antibiotic group, the activity of pepsin in jejunum of citrus extract group was significantly increased (P<0.05), and the amylase activity was significantly decreased (P<0.05); compared with the control group, the activity of trypsin in jejunum of citrus extract group was significantly increased (P<0.05). Compared with the control group, the activity of amylase in ileum of citrus extract group was significantly decreased (P<0.05). 3) There were no significant differences in intestinal ammonia nitrogen content and fecal nitrogen and phosphorus contents among all groups (P>0.05). 4) There were no significant differences in fecal ammonia and hydrogen sulfide contents among all groups (P>0.05). In conclusion, citrus extract can improve the intestinal protease and lipase activities of finishing pigs, decrease the intestinal amylase activity, and has no negative effect on intestinal antioxidant capacity, fecal nitrogen, phosphorus and odor emission.

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