反刍与草食动物营养与饲料 RUMINANT AND HERBIVORE NUTRITION AND FEED

饲粮中添加不同比例全株桑枝叶对育肥湖羊生长性能、养分表观消化率、血清抗氧化指标和瘤胃发酵参数的影响

  • 寇宇斐 ,
  • 朱文斌 ,
  • 李飞 ,
  • 李发弟 ,
  • 翁秀秀 ,
  • 唐德富 ,
  • 郝生燕 ,
  • 袁律峰
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  • 1. 兰州大学草地农业科技学院, 草地农业生态系统国家重点实验室, 农业农村部草牧业创新重点实验室, 兰州 730020;
    2. 甘肃省肉羊繁育生物技术工程实验室, 民勤 733300;
    3. 甘肃农业大学动物科学技术学院, 兰州 730070;
    4. 甘肃省农业科学院畜草与绿色农业研究所, 兰州 730070
寇宇斐(1990-),男,河南南阳人,硕士研究生,从事反刍动物营养研究。E-mail:1669764065@qq.com

收稿日期: 2020-10-30

  网络出版日期: 2021-05-14

基金资助

公益性行业(农业)科研专项"北方农作物秸秆饲用化利用技术研究与示范"(201503134);甘肃省重大科技计划"优质胴体生产和羊肉品质提升技术研究及饲料产品开发"(1602NKDH020-04)

Effects of Diets with Different Proportions of Whole Mulberry on Growth Performance, Nutrient Apparent Digestibility, Serum Antioxidant Indexes and Rumen Fermentation Parameters of Fattening Hu Sheep

  • KOU Yufei ,
  • ZHU Wenbin ,
  • LI Fei ,
  • LI Fadi ,
  • WENG Xiuxiu ,
  • TANG Defu ,
  • HAO Shengyan ,
  • YUAN Lyufeng
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  • 1. State Key Laboratory of Grassland Agro-Ecosystem, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
    2. Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China;
    3. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    4. Institute of Animal Husbandry, Pasture and Green Agriculture, Gansu Academy of Agricultural Science, Lanzhou 730070, China

Received date: 2020-10-30

  Online published: 2021-05-14

Supported by

 

摘要

本试验旨在研究饲粮中添加不同比例全株桑枝叶对育肥湖羊生长性能、养分表观消化率、血清抗氧化指标及瘤胃发酵参数的影响。选取54只健康状况良好的3月龄湖羊公羔[初始体重为(25.02±1.81)kg],随机分为3个组,每个组设18个重复,每个重复1只湖羊公羔。3组试验羊分别饲喂全株桑枝叶添加比例为0(CON组)、4%(MUL4组)和8%(MUL8组)的试验饲粮。整个试验期包括7 d的预试期和63 d的正试期。结果表明:1)各组之间终末体重、干物质采食量(DMI)和料重比(F/G)均无显著差异(P>0.05)。随着饲粮中全株桑枝叶添加比例的增加,育肥湖羊的平均日增重呈一次线性增加(P<0.05),MUL8组的平均日增重显著高于CON组(P<0.05)。2)饲粮中全株桑枝叶添加比例对育肥湖羊的干物质(DM)、有机物(OM)、粗蛋白质(CP)、粗脂肪(EE)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)表观消化率均无显著影响(P>0.05)。3)随着饲粮中全株桑枝叶添加比例的增加,育肥湖羊血清谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)均呈一次线性增加(P<0.05),其中MUL8组血清T-AOC显著高于CON组(P<0.05),MUL4组、MUL8组血清GSH-Px活性显著高于CON组(P<0.05)。育肥湖羊血清丙二醛(MDA)含量、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性各组之间差异不显著(P>0.05)。4)随着饲粮中全株桑枝叶添加比例的增加,育肥湖羊瘤胃液氨态氮(NH3-N)浓度呈一次线性增加(P<0.05),其中MUL4组和MUL8组瘤胃液NH3-N浓度显著高于CON组(P<0.05)。育肥湖羊瘤胃液总挥发性脂肪酸(TVFA)、乙酸、丙酸、异丁酸、丁酸、异戊酸、戊酸浓度以及乙丙比、pH各组之间均无显著差异(P>0.05)。综上所述,在本试验条件下,饲粮中添加4%或8%的全株桑枝叶均未对育肥湖羊的养分表观消化率和瘤胃液挥发性脂肪酸浓度产生显著影响,但添加8%的全株桑枝叶可以提高育肥湖羊的平均日增重和抗氧化能力。

本文引用格式

寇宇斐 , 朱文斌 , 李飞 , 李发弟 , 翁秀秀 , 唐德富 , 郝生燕 , 袁律峰 . 饲粮中添加不同比例全株桑枝叶对育肥湖羊生长性能、养分表观消化率、血清抗氧化指标和瘤胃发酵参数的影响[J]. 动物营养学报, 2021 , 33(5) : 2776 -2785 . DOI: 10.3969/j.issn.1006-267x.2021.05.036

Abstract

The purpose of this experiment was to study the effects of diets with different proportions of whole mulberry on growth performance, nutrient apparent digestibility, serum antioxidant indexes and rumen fermentation parameters of fattening Hu sheep. Fifty-four 3-month-old healthy male Hu sheep with the initial body weight of (25.02±1.81) kg were randomly divided into 3 groups with 18 repetitions each group and 1 Hu sheep each repetition. Hu sheep in the 3 groups were diets with the adding proportions of 0 (CON group), 4% (MUL4 group) and 8% whole mulberry (MUL8 group), respectively. The whole experimental period included the pre-feeding period of 7 days and the formal period of 63 days. The results showed as follows:1) There were no significant differences in the final body weight, dry matter intake (DMI), feed/gain (F/G) of fattening Hu sheep among groups (P>0.05). With the dietary whole mulberry proportion increasing, the average daily gain (ADG) of fattening Hu sheep was increased linearly (P<0.05), and the ADG in MUL8 group was significantly higher than that in CON group (P<0.05). 2) Dietary whole mulberry proportion had no significant differences in the apparent digestibility of dry matter (DM), organic matter (OM), ether extract (EE), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of fattening Hu sheep (P>0.05). 3) With dietary whole mulberry proportion increasing, the serum glutathion peroxidase (GSH-Px) activity and total antioxidant capacity (T-AOC) of fattening Hu sheep were increased linearly (P<0.05), the serum T-AOC in MUL8 group was significantly higher than that in CON group (P<0.05), and the serum GSH-Px activity in MUL4 group and MUL8 group was significantly higher than that in CON group (P<0.05). There were no significant differences in the serum malondialdehyde (MDA) content, catalase (CAT) and superoxide dismutase (SOD) activities of fattening Hu sheep among groups (P>0.05). 4) With dietary whole mulberry proportion increasing, the concentration of NH3-N in rumen fluid was increased linearly (P<0.05), and the rumen fluid NH3-N concentration in MUL4 group and MUL8 group was significantly higher than that in CON group (P<0.05). There were no significant differences in the concentration of total volatile fatty acids, acetate, propionate, isobutyrate, butyrate, isovalerate, valerate and acetate/propionate in rumen fluid among groups (P>0.05). The results indicate that under the conditions of this experiment, adding 4% or 8% whole mulberry in diets do not significantly affect the nutrient apparent digestibility and volatile fatty acids in rumen fluid of fattening Hu sheep, but 8% whole mulberry can improve the average daily gain and antioxidant capacity of fattening Hu sheep.

参考文献

[1] HE L W,ZHOU W,WANG C,et al.Effect of cellulase and Lactobacillus casei on ensiling characteristics,chemical composition,antioxidant activity,and digestibility of mulberry leaf silage[J].Journal of Dairy Science,2019,102(11):9919-9931.  
[2] 姚芳,倪吾钟,杨肖娥.桑树的种质资源、生态适应性及其应用前景[J].科技通报,2004,20(4):289-292,297. YAO F,NI W Z,YANG X E.Genotype resources and ecological adaptation of mulberry plants (Morus indica L.) and their application foreground[J].Bulletin of Science and Technology,2004(4):289-292,297.(in Chinese)
[3] SÁNCHEZ-SALCEDO E M,AMOROS A,HERNANDEZ F,et al.Phytochemical properties of white (Morus alba) and black (Morus nigra) mulberry leaves,a new food supplement[J].Journal of Food and Nutrition Research,2017,5(4):253-261.
[4] THAIPITAKWONG T,NUMHOM S,ARAMWIT P.Mulberry leaves and their potential effects against cardiometabolic risks:a review of chemical compositions,biological properties and clinical efficacy[J].Pharmaceutical Biology,2018,56(1):109-118.  
[5] 汪文忠.饲料用桑的营养特性及其开发利用现状[J].饲料广角,2017(12):42-43. WANG W Z.Nutrient characteristics and development and utilization status of mulberry for feed[J].Feed China,2017(12):42-43.(in Chinese)
[6] OUYANG J L,WANG M Z,HOU Q R,et al.Effects of dietary mulberry leaf powder in concentrate on the rumen fermentation and ruminal epithelium in fattening Hu sheep[J].Animals,2019,9(5):218.
[7] 王永昌,翟双双,李孟孟,等.不同产地桑枝茎叶营养成分分析及四川白鹅对其养分利用率的测定[J].中国饲料,2016(16):18-22,27. WANG Y C,ZHAI S S,LI M M,et al.Analysis of nutritional components of mulberry twig leaves from different producing areas and the nutrient utilization efficiency in Sichuan white geese[J].China Feed,2016,(16):18-22,27.(in Chinese)
[8] RODRIGUES E L,MARCELINO G,SILVA G T,et al.Nutraceutical and medicinal potential of the Morus species in metabolic dysfunctions[J].International Journal of Molecular Sciences,2019,20(2):301.
[9] 胡玥婵,袁娅利,郭丽彬,等.桑叶总黄酮提取工艺优化及体外抗氧化研究[J].现代园艺,2019(12):4-7. HU Y C,YUAN Y L,GUO L B,et al.Optimization of extraction technology of total flavonoids from Mulberry leaves and study on antioxidant activity in vitro[J].Xiandai Horticulture,2019(12):4-7.(in Chinese)
[10] HE X Y,CHEN X,OU X Q,et al.Evaluation of flavonoid and polyphenol constituents in mulberry leaves using HPLC fingerprint analysis[J].International Journal of Food Science and Technology,2020,55(2):526-533.  
[11] CUI H,LU T H,WANG M X,et al.Flavonoids from Morus alba L. leaves:optimization of extraction by response surface methodology and comprehensive evaluation of their antioxidant,antimicrobial,and inhibition of α-amylase activities through analytical hierarchy process[J].Molecules,2019,24(13):2398.
[12] 李建茂,黄僚,朱剑楠,等.广西全年条桑收获机械化模式初探[J].广西农业机械化,2018(6):30-32. LI J M,HUANG L,ZHU J N,et al.A preliminary study on the mechanization mode of harvest of annual striped mulberry in Guangxi[J].Guangxi Agricultural Mechanization,2018(6):30-32.(in Chinese)
[13] DENG H F,LIU H L,WU B X,et al.Preliminary study on production performance of different varieties of forage mulberry[J].Animal Husbandry and Feed Science,2016,8(4):230-233.
[14] 王红林,左艳春,周晓康,等.高密度草本化栽培对饲料桑全株产量及品质的影响[J].草业科学,2020,37(5):952-962. WANG H L,ZUO C Y,ZHOU K X,et al.Influence of high planting density herbal cultivating on yield and quality of whole-plant mulberry (Morus alba)[J].Pratacultural Science,2020,37(5):952-962.(in Chinese)
[15] 王加启,于建国.饲料分析与检验[M].北京:中国计量出版社,2004. WANG J Q,YU J G.Feed analysis and inspection[M].Beijing:China Metrology Press,2004.(in Chinese)
[16] 王世琴.补饲料NDF水平对哺乳羔羊消化道发育的影响[D].硕士学位论文.兰州:甘肃农业大学,2013. WANG S Q.Effect of NDF levels of supplementing feed on digestive tract development of lambs[D].Master's Thesis.Lanzhou:Gansu Agricultural University,2013.(in Chinese)
[17] 吕佳颖.粗饲料来源对育肥羔羊生产性能和瘤胃微生物区系的影响[D].硕士学位论文.兰州:兰州大学,2017. LYU J Y.Effects of roughage sources on production performance and ruminal microflora of finishing lamb[D].Master's Thesis.Lanzhou:Lanzhou University,2017.(in Chinese)
[18] 吴浩.桑叶和DDGS在反刍动物饲养中的应用研究[D].博士学位论文.北京:中国农业大学,2015. WU H.Application of mulberry (Morus alba) leaves and dried distillers grains with soluble on ruminant feeding[D].Ph.D.Thesis.Beijing:China Agricultural University,2015.(in Chinese)
[19] 蔡明.桑叶作为动物饲料的安全性及饲用价值评价研究[D].博士学位论文.兰州:兰州大学,2019. CAI M.Study on evaluation of safety and feeding value of mulberry (Morus alba) leaves as animal feed[D].Ph.D.Thesis.Lanzhou:Lanzhou University,2017.(in Chinese)
[20] DONG Z H,WANG S R,ZHAO J,et al.Effects of additives on the fermentation quality,in vitro digestibility and aerobic stability of mulberry (Morus alba L.) leaves silage[J].Asian-Australasian Journal of Animal Sciences,2020,33(8):1292-1300.  
[21] 杨静,李同洲,曹洪战,等.不同水平饲用桑粉对育肥猪生长性能和肉质的影响[J].中国畜牧杂志,2014,50(7):52-56. YANG J,LI T Z,CAO H Z,et al.Effects of dietary forage mulberry on growth performance and meat quality of finishing pigs[J].Chinese Journal of Animal Science,2014,50(7):52-56.(in Chinese)
[22] ZHOU Z M,ZHOU B,REN L P,et al.Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on finishing steer growth performance,blood biochemical parameters,and carcass characteristics[J].PLoS One,2014,9(1):e85406..
[23] 李伟玲.桑叶对肉羊生产性能、血液生化指标、免疫抗氧化功能和肉品质的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2012. LI W L.Effect of mulberry leaves on performance,serum biochemical parameters,immune function,antioxidation and meat quality in meat sheep[D].Master's Thesis.Hohhot:Inner Mongolia Agricultural University,2012.(in Chinese)
[24] 冯丹,欧阳佳良,王梦芝,等.饲粮中不同比例桑叶粉对湖羊瘤胃上皮组织结构的影响[J].动物营养学报,2017,29(6):2196-2203. FENG D,OU YANG J L,WANG M Z,et al.Effects of different proportions of mulberry leaf powder in diet on rumen epithelium structure of Hu sheep[J].Chinese Journal of Animal Nutrition,2017,29(6):2196-2203.(in Chinese)
[25] WANG B,YANG C T,DIAO Q Y,et al.The influence of mulberry leaf flavonoids and Candida tropicalis on antioxidant function and gastrointestinal development of preweaning calves challenged with Escherichia coli O141:K99[J].Journal of Dairy Science,2018,101(7):6098-6108.  
[26] 范京辉,张永华,楼立峰,等.桑叶及其提取物对AA肉鸡生长、养分消化与胴体品质的影响[J].杭州农业与科技,2012(5):29-32. FAN J H,ZHANG Y H,LOU L F,et al.Effects of mulberry leaf and its extracts on growth,nutrient digestion and carcass quality of AA broiler chickens[J].Hangzhou Agricultural Science and Technology,2012(5):29-32.(in Chinese)
[27] SALINAS-CHAVIRA J,CASTILLO-MARTÍNEZ O,RAMIREZ-BRIBIESCA J E,et al.Effect of increasing levels of white mulberry leaves (Morus alba) on ruminal dry matter degradability in lambs[J].Tropical Animal Health and Production,2011,43(5):995-999.  
[28] 许倩倩.桑叶与异戊酸对晋南牛瘤胃发酵及养分消化利用的影响[D].硕士学位论文.太谷:山西农业大学,2016. XU Q Q.Effect of the mulberry leaves and isovaleric acid on rumen fermentation and nutrient digestion and utilization in Jinnan cattle[D].Master's Thesis.Taigu:Shanxi Agricultural University,2016.(in Chinese)
[29] 吕冠霖.利用甘蔗叶和蛋白桑树配制生长肉牛全混合型压块日粮研究[D].硕士学位论文.南宁:广西大学,2015. LYU G S.Study on confecting thee full mixed type briquetting daily ration feeding growing bulls using the sugarcane leaves & protein mulberry[D].Master's Thesis.Nanning:Guangxi University,2015.(in Chinese)
[30] 王润娟.玉米秸秆保质贮存及纤维素高效水解方法的研究[D].硕士学位论文.天津:天津大学,2012. WANG R J.Research on quality storage of corn stalk and efficient hydrolysis of cellulose[D].Master's Thesis.Tianjin:Tianjin University,2012.(in Chinese)
[31] YANG W Z,BEAUCHEMIN K A.Physically effective fiber:method of determination and effects on chewing,ruminal acidosis,and digestion by dairy cows[J].Journal of Dairy Science,2006,89(7):2618-2633.  
[32] ŚPITALNIAK-BAJERSKA K,SZUMNY A,POGODA-SEWERNIAK K,et al.Effects of n-3 fatty acids on growth,antioxidant status,and immunity of preweaned dairy calves[J].Journal of Dairy Science,2020,103(3):2864-2876.  
[33] 罗阳,李昊帮,肖建中,等.发酵桑叶对湘西黄牛×利木赞杂交F1代育肥牛屠宰性能、肉品质及肌肉中氨基酸、脂肪酸含量的影响[J].动物营养学报,2020,32(1):244-252. LUO Y,LI H B,XIAO J Z,et al.Effects of fermented mulberry leaves on serum biochemical,antioxidant and immune indexes of Xiangxi yellow cattle×Limousin hybrid F1 fattening bulls[J].Chinese Journal of Animal Nutrition,2020,32(1):244-252.(in Chinese)
[34] JIA Z S,TANG M C,WU J M.The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals[J].Food Chemistry,1999,64(4):555-559.  
[35] WEN L R,SHI D D,ZHOU T,et al.Identification of two novel prenylated flavonoids in mulberry leaf and their bioactivities[J].Food Chemistry,2020,315:126236.
[36] KUMAR J P,MANDAL B B.Antioxidant potential of mulberry and non-mulberry silk sericin and its implications in biomedicine[J].Free Radical Biology and Medicine,2017,108:803-818.
[37] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004. FENG Y L.Ruminant nutrition[M].Beijing:Science Press,2004.(in Chinese)
[38] THAO N T,WANAPAT M,CHERDTHONG A,et al.Effects of eucalyptus crude oils supplementation on rumen fermentation, microorganism and nutrient digestibility in swamp buffaloes[J].Asian-Australasian Journal of Animal Sciences,2014,27(1):46-54.  
[39] WANG C,LIU Q,GUO G,et al.Effects of rumen-protected folic acid and branched-chain volatile fatty acids supplementation on lactation performance,ruminal fermentation,nutrient digestion and blood metabolites in dairy cows[J].Animal Feed Science and Technology,2019,247:157-165.
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